O57203 (KITH_VACCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	TK
Ordered Locus Names:	MVA086R, ACAM3000_MVA_086
ORF Names:	J2R

Organism

Vaccinia virus (strain Ankara) (VACV)

Taxonomic identifier

126794 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphorylates thymidine and thymidine analogs, such as azidothymidine (AZT). Part of the salvage pathway for pyrimidine deoxyribonucleotide synthesis. Ref.3
Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. Ref.3
Subunit structure	Homotetramer By similarity. Two molecules of substrate bind to each enzyme tetramer By similarity. Ref.3
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 177

177

Thymidine kinase

PRO_0000174937

Regions

Nucleotide binding

11 – 18

8

ATP Probable

Region

157 – 161

5

Substrate binding

Sites

Active site

83

1

Proton acceptor Potential

Metal binding

138

1

Zinc

Metal binding

141

1

Zinc

Metal binding

170

1

Zinc

Metal binding

173

1

Zinc

Binding site

113

1

Substrate; via amide nitrogen

Secondary structure

1

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177

Helix Strand Turn

Details...

Beta strand

5 – 10

6

Helix

17 – 29

13

Turn

30 – 32

3

Beta strand

35 – 40

6

Helix

66 – 69

4

Turn

70 – 72

3

Helix

73 – 75

3

Beta strand

77 – 82

6

Helix

84 – 86

3

Helix

90 – 99

10

Beta strand

103 – 107

5

Turn

119 – 121

3

Helix

122 – 126

5

Beta strand

129 – 133

5

Turn

139 – 141

3

Beta strand

143 – 145

3

Beta strand

147 – 153

7

Turn

163 – 165

3

Beta strand

166 – 169

4

Helix

171 – 174

4

Sequences

	Sequence		Length	Mass (Da)
	O57203 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 57E19BCEBE6F3C54	FASTA	177	20,028
	10 20 30 40 50 60 MNGGHIQLII GPMFSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL WTHDKNNFEA 70 80 90 100 110 120 LEATKLCDVL ESITDFSVIG IDEGQFFPDI VEFCERMANE GKIVIVAALD GTFQRKPFNN 130 140 150 160 170 ILNLIPLSEM VVKLTAVCMK CFKEASFSKR LGEETEIEII GGNDMYQSVC RKCYVGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses." Antoine G., Scheiflinger F., Dorner F., Falkner F.G. Virology 244:365-396(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J., Khristova M., Wohlhueter R.M. Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Isolate Acambis 3000.
[3]	"Structure of vaccinia virus thymidine kinase in complex with dTTP: insights for drug design." El Omari K., Solaroli N., Karlsson A., Balzarini J., Stammers D.K. BMC Struct. Biol. 6:22-22(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) IN COMPLEX WITH ZINC IONS AND TTP, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U94848 Genomic DNA. Translation: AAB96503.1.
AY603355 Genomic DNA. Translation: AAT10484.1.

PIR

T37362.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2J87	X-ray	3.10	A/B/C/D	1-177	[»]

ProteinModelPortal

O57203.

SMR

O57203. Positions 4-176.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BRENDA

2.7.1.21. 6591.

Family and domain databases

InterPro

IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]

PANTHER

PTHR11441. PTHR11441. 1 hit.

Pfam

PF00265. TK. 1 hit.
[Graphical view]

PIRSF

PIRSF035805. TK_cell. 1 hit.

SUPFAM

SSF52540. SSF52540. 1 hit.

PROSITE

PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O57203.

Entry information

Entry name

KITH_VACCA

Accession

Primary (citable) accession number: O57203
Secondary accession number(s): Q6J3E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	June 1, 1998
Last modified:	May 29, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program