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O57203

- KITH_VACCA

UniProt

O57203 - KITH_VACCA

Protein

Thymidine kinase

Gene

TK

Organism
Vaccinia virus (strain Ankara) (VACV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Phosphorylates thymidine and thymidine analogs, such as azidothymidine (AZT). Part of the salvage pathway for pyrimidine deoxyribonucleotide synthesis.1 Publication

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei83 – 831Proton acceptorSequence Analysis
    Binding sitei113 – 1131Substrate; via amide nitrogen
    Metal bindingi138 – 1381Zinc
    Metal bindingi141 – 1411Zinc
    Metal bindingi170 – 1701Zinc
    Metal bindingi173 – 1731Zinc

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 188ATPCurated

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. thymidine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BRENDAi2.7.1.21. 6591.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase (EC:2.7.1.21)
    Gene namesi
    Name:TK
    Ordered Locus Names:MVA086R, ACAM3000_MVA_086
    ORF Names:J2R
    OrganismiVaccinia virus (strain Ankara) (VACV)
    Taxonomic identifieri126794 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 177177Thymidine kinasePRO_0000174937Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer. Two molecules of substrate bind to each enzyme tetramer.By similarity

    Structurei

    Secondary structure

    1
    177
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 106
    Helixi17 – 2913
    Turni30 – 323
    Beta strandi35 – 406
    Helixi66 – 694
    Turni70 – 723
    Helixi73 – 753
    Beta strandi77 – 826
    Helixi84 – 863
    Helixi90 – 9910
    Beta strandi103 – 1075
    Turni119 – 1213
    Helixi122 – 1265
    Beta strandi129 – 1335
    Turni139 – 1413
    Beta strandi143 – 1453
    Beta strandi147 – 1537
    Turni163 – 1653
    Beta strandi166 – 1694
    Helixi171 – 1744

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2J87X-ray3.10A/B/C/D1-177[»]
    ProteinModelPortaliO57203.
    SMRiO57203. Positions 4-176.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO57203.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni157 – 1615Substrate binding

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Family and domain databases

    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O57203-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNGGHIQLII GPMFSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL    50
    WTHDKNNFEA LEATKLCDVL ESITDFSVIG IDEGQFFPDI VEFCERMANE 100
    GKIVIVAALD GTFQRKPFNN ILNLIPLSEM VVKLTAVCMK CFKEASFSKR 150
    LGEETEIEII GGNDMYQSVC RKCYVGS 177
    Length:177
    Mass (Da):20,028
    Last modified:June 1, 1998 - v1
    Checksum:i57E19BCEBE6F3C54
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U94848 Genomic DNA. Translation: AAB96503.1.
    AY603355 Genomic DNA. Translation: AAT10484.1.
    PIRiT37362.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U94848 Genomic DNA. Translation: AAB96503.1 .
    AY603355 Genomic DNA. Translation: AAT10484.1 .
    PIRi T37362.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2J87 X-ray 3.10 A/B/C/D 1-177 [» ]
    ProteinModelPortali O57203.
    SMRi O57203. Positions 4-176.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 2.7.1.21. 6591.

    Miscellaneous databases

    EvolutionaryTracei O57203.

    Family and domain databases

    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses."
      Antoine G., Scheiflinger F., Dorner F., Falkner F.G.
      Virology 244:365-396(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J., Khristova M., Wohlhueter R.M.
      Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Isolate Acambis 3000.
    3. "Structure of vaccinia virus thymidine kinase in complex with dTTP: insights for drug design."
      El Omari K., Solaroli N., Karlsson A., Balzarini J., Stammers D.K.
      BMC Struct. Biol. 6:22-22(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) IN COMPLEX WITH ZINC IONS AND TTP, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.

    Entry informationi

    Entry nameiKITH_VACCA
    AccessioniPrimary (citable) accession number: O57203
    Secondary accession number(s): Q6J3E4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 2, 2002
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3