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Protein

Thymidine kinase

Gene

TK

Organism
Vaccinia virus (strain Ankara) (VACV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates thymidine and thymidine analogs, such as azidothymidine (AZT). Part of the salvage pathway for pyrimidine deoxyribonucleotide synthesis.1 Publication

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei83Proton acceptorCombined sources1 Publication1
Binding sitei113Substrate; via amide nitrogenCombined sources1 Publication1
Metal bindingi138ZincCombined sources1 Publication1
Metal bindingi141ZincCombined sources1 Publication1
Metal bindingi170ZincCombined sources1 Publication1
Metal bindingi173ZincCombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 18ATPCombined sources1 Publication8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.21. 6591.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
Ordered Locus Names:MVA086R, ACAM3000_MVA_086
ORF Names:J2R
OrganismiVaccinia virus (strain Ankara) (VACV)
Taxonomic identifieri126794 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001749371 – 177Thymidine kinaseAdd BLAST177

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi170Interchain (with C-173)Combined sources1 Publication
Disulfide bondi173Interchain (with C-170)Combined sources1 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homotetramer. Two molecules of substrate bind to each enzyme tetramer.By similarity

Structurei

Secondary structure

1177
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Helixi17 – 29Combined sources13
Turni30 – 32Combined sources3
Beta strandi35 – 40Combined sources6
Beta strandi56 – 58Combined sources3
Helixi66 – 69Combined sources4
Turni70 – 72Combined sources3
Helixi73 – 75Combined sources3
Beta strandi77 – 82Combined sources6
Helixi84 – 86Combined sources3
Helixi90 – 99Combined sources10
Beta strandi103 – 107Combined sources5
Turni119 – 121Combined sources3
Helixi122 – 126Combined sources5
Beta strandi129 – 133Combined sources5
Turni139 – 141Combined sources3
Beta strandi143 – 145Combined sources3
Beta strandi147 – 153Combined sources7
Turni163 – 165Combined sources3
Beta strandi166 – 169Combined sources4
Helixi171 – 174Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J87X-ray3.10A/B/C/D1-177[»]
ProteinModelPortaliO57203.
SMRiO57203.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO57203.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni157 – 161Substrate bindingCombined sources1 Publication5

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O57203-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGGHIQLII GPMFSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL
60 70 80 90 100
WTHDKNNFEA LEATKLCDVL ESITDFSVIG IDEGQFFPDI VEFCERMANE
110 120 130 140 150
GKIVIVAALD GTFQRKPFNN ILNLIPLSEM VVKLTAVCMK CFKEASFSKR
160 170
LGEETEIEII GGNDMYQSVC RKCYVGS
Length:177
Mass (Da):20,028
Last modified:June 1, 1998 - v1
Checksum:i57E19BCEBE6F3C54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94848 Genomic DNA. Translation: AAB96503.1.
AY603355 Genomic DNA. Translation: AAT10484.1.
PIRiT37362.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94848 Genomic DNA. Translation: AAB96503.1.
AY603355 Genomic DNA. Translation: AAT10484.1.
PIRiT37362.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J87X-ray3.10A/B/C/D1-177[»]
ProteinModelPortaliO57203.
SMRiO57203.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.7.1.21. 6591.

Miscellaneous databases

EvolutionaryTraceiO57203.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKITH_VACCA
AccessioniPrimary (citable) accession number: O57203
Secondary accession number(s): Q6J3E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.