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O57203

- KITH_VACCA

UniProt

O57203 - KITH_VACCA

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Protein

Thymidine kinase

Gene

TK

Organism
Vaccinia virus (strain Ankara) (VACV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Phosphorylates thymidine and thymidine analogs, such as azidothymidine (AZT). Part of the salvage pathway for pyrimidine deoxyribonucleotide synthesis.1 Publication

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei83 – 831Proton acceptorSequence Analysis
Binding sitei113 – 1131Substrate; via amide nitrogen
Metal bindingi138 – 1381Zinc
Metal bindingi141 – 1411Zinc
Metal bindingi170 – 1701Zinc
Metal bindingi173 – 1731Zinc

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 188ATPCurated

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. thymidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.21. 6591.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
Ordered Locus Names:MVA086R, ACAM3000_MVA_086
ORF Names:J2R
OrganismiVaccinia virus (strain Ankara) (VACV)
Taxonomic identifieri126794 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 177177Thymidine kinasePRO_0000174937Add
BLAST

Interactioni

Subunit structurei

Homotetramer. Two molecules of substrate bind to each enzyme tetramer.By similarity

Structurei

Secondary structure

1
177
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 106Combined sources
Helixi17 – 2913Combined sources
Turni30 – 323Combined sources
Beta strandi35 – 406Combined sources
Helixi66 – 694Combined sources
Turni70 – 723Combined sources
Helixi73 – 753Combined sources
Beta strandi77 – 826Combined sources
Helixi84 – 863Combined sources
Helixi90 – 9910Combined sources
Beta strandi103 – 1075Combined sources
Turni119 – 1213Combined sources
Helixi122 – 1265Combined sources
Beta strandi129 – 1335Combined sources
Turni139 – 1413Combined sources
Beta strandi143 – 1453Combined sources
Beta strandi147 – 1537Combined sources
Turni163 – 1653Combined sources
Beta strandi166 – 1694Combined sources
Helixi171 – 1744Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2J87X-ray3.10A/B/C/D1-177[»]
ProteinModelPortaliO57203.
SMRiO57203. Positions 4-176.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO57203.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 1615Substrate binding

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O57203-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNGGHIQLII GPMFSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL
60 70 80 90 100
WTHDKNNFEA LEATKLCDVL ESITDFSVIG IDEGQFFPDI VEFCERMANE
110 120 130 140 150
GKIVIVAALD GTFQRKPFNN ILNLIPLSEM VVKLTAVCMK CFKEASFSKR
160 170
LGEETEIEII GGNDMYQSVC RKCYVGS
Length:177
Mass (Da):20,028
Last modified:June 1, 1998 - v1
Checksum:i57E19BCEBE6F3C54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94848 Genomic DNA. Translation: AAB96503.1.
AY603355 Genomic DNA. Translation: AAT10484.1.
PIRiT37362.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94848 Genomic DNA. Translation: AAB96503.1 .
AY603355 Genomic DNA. Translation: AAT10484.1 .
PIRi T37362.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2J87 X-ray 3.10 A/B/C/D 1-177 [» ]
ProteinModelPortali O57203.
SMRi O57203. Positions 4-176.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 2.7.1.21. 6591.

Miscellaneous databases

EvolutionaryTracei O57203.

Family and domain databases

InterProi IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view ]
PANTHERi PTHR11441. PTHR11441. 1 hit.
Pfami PF00265. TK. 1 hit.
[Graphical view ]
PIRSFi PIRSF035805. TK_cell. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses."
    Antoine G., Scheiflinger F., Dorner F., Falkner F.G.
    Virology 244:365-396(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J., Khristova M., Wohlhueter R.M.
    Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate Acambis 3000.
  3. "Structure of vaccinia virus thymidine kinase in complex with dTTP: insights for drug design."
    El Omari K., Solaroli N., Karlsson A., Balzarini J., Stammers D.K.
    BMC Struct. Biol. 6:22-22(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) IN COMPLEX WITH ZINC IONS AND TTP, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.

Entry informationi

Entry nameiKITH_VACCA
AccessioniPrimary (citable) accession number: O57203
Secondary accession number(s): Q6J3E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 1998
Last modified: November 26, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3