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Protein

Poly(A) polymerase catalytic subunit

Gene

PAPL

Organism
Vaccinia virus (strain Ankara) (VACV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's.

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei202 – 2021By similarity
Active sitei204 – 2041By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. polynucleotide adenylyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing, Transcription

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase catalytic subunit (EC:2.7.7.19)
Alternative name(s):
Poly(A) polymerase large subunit
Short name:
PAP-L
VP55
Gene namesi
Name:PAPL
Ordered Locus Names:MVA048L, ACAM3000_MVA_048
OrganismiVaccinia virus (strain Ankara) (VACV)
Taxonomic identifieri126794 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Poly(A) polymerase catalytic subunitPRO_0000099105Add
BLAST

Interactioni

Subunit structurei

Heterodimer of a large (catalytic) subunit and a small (regulatory) subunit.

Structurei

3D structure databases

ProteinModelPortaliO57184.
SMRiO57184. Positions 12-479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR024231. Pox_polyA_pol_nucTrfase.
IPR024397. Poxvirus_polyA_pol_cat_C.
IPR024398. Poxvirus_polyA_pol_cat_N.
[Graphical view]
PfamiPF03296. Pox_polyA_pol. 1 hit.
PF12629. Pox_polyA_pol_C. 1 hit.
PF12630. Pox_polyA_pol_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O57184-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRNPDQNTF PNITLKIIET YLGRVPSVNE YHMLKLQARN IQKITVFNKD
60 70 80 90 100
IFVSLVKKNK KRFFSDVDTS ASEIKDRILS YFSKQTQTYN IGKLFTIIEL
110 120 130 140 150
QSVLVTTYTD ILGVLTIKAP NVISSKISYN VTSMEELARD MLNSMNVAVI
160 170 180 190 200
DKAKVMGRHN VSSLVKNVNK LMEEYLRRHN KSCICYGSYS LYLINPNIRY
210 220 230 240 250
GDIDILQTNS RTFLIDLAFL IKFITGNNII LSKIPYLRNY MVIKDENDNH
260 270 280 290 300
IIDSFNIRQD TMNVVPKIFI DNIYIVDPTF QLLNMIKMFS QIDRLEDLSK
310 320 330 340 350
DPEKFNARMA TMLEYVRYTH GIVFDGKRNN MPMKCIIDEN NRIVTVTTKD
360 370 380 390 400
YFSFKKCLVY LDENVLSSDI LDLNADTSCD FESVTNSVYL IHDNIMYTYF
410 420 430 440 450
SNTILLSDKG KVHEISARGL CAHILLYQML TSGEYKQCLS DLLNSMMNRD
460 470
KIPIYSHTER DKKPGRHGFI NIEKDIIVF
Length:479
Mass (Da):55,566
Last modified:June 1, 1998 - v1
Checksum:iC90B9FE5EB62B6DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94848 Genomic DNA. Translation: AAB96426.1.
AY603355 Genomic DNA. Translation: AAT10446.1.
PIRiT30794.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94848 Genomic DNA. Translation: AAB96426.1.
AY603355 Genomic DNA. Translation: AAT10446.1.
PIRiT30794.

3D structure databases

ProteinModelPortaliO57184.
SMRiO57184. Positions 12-479.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR024231. Pox_polyA_pol_nucTrfase.
IPR024397. Poxvirus_polyA_pol_cat_C.
IPR024398. Poxvirus_polyA_pol_cat_N.
[Graphical view]
PfamiPF03296. Pox_polyA_pol. 1 hit.
PF12629. Pox_polyA_pol_C. 1 hit.
PF12630. Pox_polyA_pol_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses."
    Antoine G., Scheiflinger F., Dorner F., Falkner F.G.
    Virology 244:365-396(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J., Khristova M., Wohlhueter R.M.
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate Acambis 3000.

Entry informationi

Entry nameiPAP1_VACCA
AccessioniPrimary (citable) accession number: O57184
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: June 1, 1998
Last modified: April 1, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.