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O57175

- RIR2_VACCA

UniProt

O57175 - RIR2_VACCA

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Protein

Ribonucleoside-diphosphate reductase small chain

Gene

MVA032L

Organism
Vaccinia virus (strain Ankara) (VACV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Iron 1PROSITE-ProRule annotation
Metal bindingi101 – 1011Iron 1PROSITE-ProRule annotation
Metal bindingi101 – 1011Iron 2By similarity
Metal bindingi104 – 1041Iron 1PROSITE-ProRule annotation
Active sitei108 – 1081PROSITE-ProRule annotation
Metal bindingi163 – 1631Iron 2By similarity
Metal bindingi197 – 1971Iron 2By similarity
Metal bindingi200 – 2001Iron 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
Ordered Locus Names:MVA032L, ACAM3000_MVA_032
OrganismiVaccinia virus (strain Ankara) (VACV)
Taxonomic identifieri126794 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319Ribonucleoside-diphosphate reductase small chainPRO_0000190495Add
BLAST

Expressioni

Inductioni

Expressed early in the viral replicative cycle.

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO57175.
SMRiO57175. Positions 2-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O57175-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEPILAPNPN RFVIFPIQYH DIWNMYKKAE ASFWTVEEVD ISKDINDWNK
60 70 80 90 100
LTPDEKYFIK HVLAFFAASD GIVNENLAER FCTEVQITEA RCFYGFQMAI
110 120 130 140 150
ENIHSEMYSL LIDTYVKDSN EKNYLFNAIE TMPCVKKKAD WAQKWIHDSA
160 170 180 190 200
GYGERLIAFA AVEGIFFSGS FASIFWLKKR GLMPGLTFSN ELISRDEGLH
210 220 230 240 250
CDFACLMFKH LLHPPSEETV RSIITDAVSI EQEFLTAALP VKLIGMNCEM
260 270 280 290 300
MKTYIEFVAD RLISELGFKK IYNVTNPFDF MENISLEGKT NFFEKRVGEY
310
QKMGVMSQED NHFSLDVDF
Length:319
Mass (Da):36,947
Last modified:June 1, 1998 - v1
Checksum:i0444D6A80EA0AD51
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U94848 Genomic DNA. Translation: AAB96415.1.
AY603355 Genomic DNA. Translation: AAT10430.1.
PIRiT30782.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U94848 Genomic DNA. Translation: AAB96415.1 .
AY603355 Genomic DNA. Translation: AAT10430.1 .
PIRi T30782.

3D structure databases

ProteinModelPortali O57175.
SMRi O57175. Positions 2-282.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
PROSITEi PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses."
    Antoine G., Scheiflinger F., Dorner F., Falkner F.G.
    Virology 244:365-396(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J., Khristova M., Wohlhueter R.M.
    Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate Acambis 3000.

Entry informationi

Entry nameiRIR2_VACCA
AccessioniPrimary (citable) accession number: O57175
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: June 1, 1998
Last modified: October 1, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3