Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O57175

- RIR2_VACCA

UniProt

O57175 - RIR2_VACCA

Protein

Ribonucleoside-diphosphate reductase small chain

Gene

MVA032L

Organism
Vaccinia virus (strain Ankara) (VACV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi70 – 701Iron 1PROSITE-ProRule annotation
    Metal bindingi101 – 1011Iron 1PROSITE-ProRule annotation
    Metal bindingi101 – 1011Iron 2By similarity
    Metal bindingi104 – 1041Iron 1PROSITE-ProRule annotation
    Active sitei108 – 1081PROSITE-ProRule annotation
    Metal bindingi163 – 1631Iron 2By similarity
    Metal bindingi197 – 1971Iron 2By similarity
    Metal bindingi200 – 2001Iron 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
    2. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase small subunit
    Gene namesi
    Ordered Locus Names:MVA032L, ACAM3000_MVA_032
    OrganismiVaccinia virus (strain Ankara) (VACV)
    Taxonomic identifieri126794 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 319319Ribonucleoside-diphosphate reductase small chainPRO_0000190495Add
    BLAST

    Expressioni

    Inductioni

    Expressed early in the viral replicative cycle.

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO57175.
    SMRiO57175. Positions 2-282.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view]
    PANTHERiPTHR23409. PTHR23409. 1 hit.
    PfamiPF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view]
    SUPFAMiSSF47240. SSF47240. 1 hit.
    PROSITEiPS00368. RIBORED_SMALL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O57175-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPILAPNPN RFVIFPIQYH DIWNMYKKAE ASFWTVEEVD ISKDINDWNK    50
    LTPDEKYFIK HVLAFFAASD GIVNENLAER FCTEVQITEA RCFYGFQMAI 100
    ENIHSEMYSL LIDTYVKDSN EKNYLFNAIE TMPCVKKKAD WAQKWIHDSA 150
    GYGERLIAFA AVEGIFFSGS FASIFWLKKR GLMPGLTFSN ELISRDEGLH 200
    CDFACLMFKH LLHPPSEETV RSIITDAVSI EQEFLTAALP VKLIGMNCEM 250
    MKTYIEFVAD RLISELGFKK IYNVTNPFDF MENISLEGKT NFFEKRVGEY 300
    QKMGVMSQED NHFSLDVDF 319
    Length:319
    Mass (Da):36,947
    Last modified:June 1, 1998 - v1
    Checksum:i0444D6A80EA0AD51
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U94848 Genomic DNA. Translation: AAB96415.1.
    AY603355 Genomic DNA. Translation: AAT10430.1.
    PIRiT30782.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U94848 Genomic DNA. Translation: AAB96415.1 .
    AY603355 Genomic DNA. Translation: AAT10430.1 .
    PIRi T30782.

    3D structure databases

    ProteinModelPortali O57175.
    SMRi O57175. Positions 2-282.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view ]
    PANTHERi PTHR23409. PTHR23409. 1 hit.
    Pfami PF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47240. SSF47240. 1 hit.
    PROSITEi PS00368. RIBORED_SMALL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses."
      Antoine G., Scheiflinger F., Dorner F., Falkner F.G.
      Virology 244:365-396(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J., Khristova M., Wohlhueter R.M.
      Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Isolate Acambis 3000.

    Entry informationi

    Entry nameiRIR2_VACCA
    AccessioniPrimary (citable) accession number: O57175
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 7, 2004
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3