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Protein

Pro-vaccinia growth factor

Gene

VGF

Organism
Vaccinia virus (strain Ankara) (VACV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Vaccinia growth factor stimulates cellular proliferation (hyperplasia) around infected cells. This effect is beneficial for virus replication in vivo, because poxviruses replicate possibly better in proliferating cells than in quiescent cells. Acts by binding host EGFR, inducing its dimerization, autophosphorylation and leading to activation of several cellular pathways regulating cell proliferation or cell survival. The activation by host EFGR of mitogen activated protein kinases (MAPK) and extracellular-signal regulated kinases (ERK) are essential for the positive effect of vaccinia growth factor on poxvirus virulence in vivo (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Growth factor

Keywords - Biological processi

Host-virus interaction

Names & Taxonomyi

Protein namesi
Recommended name:
Pro-vaccinia growth factor
Short name:
Pro-VGF
Cleaved into the following chain:
Vaccinia growth factor
Short name:
VGF
Alternative name(s):
Secreted epidermal growth factor-like
Gene namesi
Name:VGF
Ordered Locus Names:MVA005R, ACAM3000_MVA_005
OrganismiVaccinia virus (strain Ankara) (VACV)
Taxonomic identifieri126794 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

Pro-vaccinia growth factor :
  • Host membrane By similarity; Single-pass type I membrane protein By similarity
Vaccinia growth factor :
  • Secreted By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 100ExtracellularSequence analysisAdd BLAST82
Transmembranei101 – 121HelicalSequence analysisAdd BLAST21
Topological domaini122 – 140CytoplasmicSequence analysisAdd BLAST19

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000000759519 – 140Pro-vaccinia growth factorAdd BLAST122
ChainiPRO_000041291419 – 96Vaccinia growth factorSequence analysisAdd BLAST78

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi34N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi45 ↔ 58PROSITE-ProRule annotation
Disulfide bondi53 ↔ 69PROSITE-ProRule annotation
Disulfide bondi71 ↔ 80PROSITE-ProRule annotation
Glycosylationi95N-linked (GlcNAc...); by hostSequence analysis1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei96 – 97Cleavage (By host protease)Sequence analysis2

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Vaccinia growth factor interacts with host EGFR and promotes EGFR dimerization.By similarity

Structurei

3D structure databases

ProteinModelPortaliO57166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 81EGF-likePROSITE-ProRule annotationAdd BLAST41

Sequence similaritiesi

Contains 1 EGF-like domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR011170. GF_C11R.
[Graphical view]
PIRSFiPIRSF001779. GF_C11R. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O57166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLINYLMLLF AAMIIRSFAD SGNAIETTSP EITNATTDIP AIRLCGPEGD
60 70 80 90 100
GYCLHGDCIH ARDIDGMYCR CSHGYTGIRC QHVVLVDYQR SEKPNTTTSY
110 120 130 140
IPSPGIMLVL VGIIIITCCL LSVYRFTRRT KLPIQDMVVP
Length:140
Mass (Da):15,532
Last modified:June 1, 1998 - v1
Checksum:iE0834FA8D51BD8BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94848 Genomic DNA. Translation: AAB96482.1.
AY603355 Genomic DNA. Translation: AAT10402.1.
PIRiT30766.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94848 Genomic DNA. Translation: AAB96482.1.
AY603355 Genomic DNA. Translation: AAT10402.1.
PIRiT30766.

3D structure databases

ProteinModelPortaliO57166.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR011170. GF_C11R.
[Graphical view]
PIRSFiPIRSF001779. GF_C11R. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVGF_VACCA
AccessioniPrimary (citable) accession number: O57166
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: June 1, 1998
Last modified: October 5, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.