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O56263

- NEP_I97A1

UniProt

O56263 - NEP_I97A1

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Protein

Nuclear export protein

Gene

NS

Organism
Influenza A virus (strain A/Hong Kong/156/1997 H5N1 genotype Gs/Gd)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs to which the M1 protein is bound. Because the M1 protein is not available in large quantities until the later stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the nucleus before sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enters the cytoplasm only when they have associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced (By similarity).By similarity

GO - Biological processi

  1. RNA export from nucleus Source: InterPro
  2. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear export protein
Short name:
NEP
Alternative name(s):
Non-structural protein 2
Short name:
NS2
Gene namesi
Name:NS
OrganismiInfluenza A virus (strain A/Hong Kong/156/1997 H5N1 genotype Gs/Gd)
Taxonomic identifieri130763 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
Homo sapiens (Human) [TaxID: 9606]
Panthera pardus (Leopard) (Felis pardus) [TaxID: 9691]
Panthera tigris (Tiger) [TaxID: 9694]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008587: Genome

Subcellular locationi

Virion Curated. Host nucleus By similarity

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
  2. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 121121Nuclear export proteinPRO_0000078991Add
BLAST

Interactioni

Subunit structurei

Binds M1 protein. May interact with human nucleoporin RAB/HRB and exportin XPO1/CRM1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO56263.
SMRiO56263. Positions 63-116.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 2110Nuclear export signalBy similarity
Motifi85 – 9410Nuclear export signal

Sequence similaritiesi

Belongs to the influenza viruses NEP family.Curated

Family and domain databases

InterProiIPR000968. Flu_NS2.
[Graphical view]
PfamiPF00601. Flu_NS2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform NEP (identifier: O56263-1) [UniParc]FASTAAdd to Basket

Also known as: NS2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSNTVSSFQ DILKRMSKMQ LGSSSEDLNG MITQFESLKL YRDSLGEAVM
60 70 80 90 100
RMGDLHSLQN RNGKWREQLS QKFEEIRWLI EEVRHRLRIT ENSFEQITFM
110 120
QALQLLLEVE QEIRTFSFQL I
Length:121
Mass (Da):14,390
Last modified:January 24, 2006 - v2
Checksum:iA5381BD935178D31
GO
Isoform NS1 (identifier: O56264-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry O56264.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:230
Mass (Da):26,237
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF036360 Genomic RNA. Translation: AAC34269.2.
AF046091 Genomic RNA. Translation: AAC32093.1.
AJ404736 Genomic RNA. Translation: CAC04090.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF036360 Genomic RNA. Translation: AAC34269.2 .
AF046091 Genomic RNA. Translation: AAC32093.1 .
AJ404736 Genomic RNA. Translation: CAC04090.1 .

3D structure databases

ProteinModelPortali O56263.
SMRi O56263. Positions 63-116.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR000968. Flu_NS2.
[Graphical view ]
Pfami PF00601. Flu_NS2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness."
    Subbarao K., Klimov A., Katz J., Regnery H., Lim W., Hall H., Perdue M., Swayne D., Bender C., Huang J., Hemphill M., Rowe T., Shaw M., Xu X., Fukuda K., Cox N.
    Science 279:393-396(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Cited for: SEQUENCE REVISION.
  3. "Comparisons of highly virulent H5N1 influenza A viruses isolated from humans and chickens from Hong Kong."
    Suarez D.L., Perdue M.L., Cox N., Rowe T., Bender C., Huang J., Swayne D.E.
    J. Virol. 72:6678-6688(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  4. "Avian-to-human transmission of H9N2 subtype influenza A viruses: relationship between H9N2 and H5N1 human isolates."
    Lin Y.P., Shaw M., Gregory V., Cameron K., Lim W., Klimov A., Subbarao K., Guan Y., Krauss S., Shortridge K., Webster R., Cox N., Hay A.
    Proc. Natl. Acad. Sci. U.S.A. 97:9654-9658(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNEP_I97A1
AccessioniPrimary (citable) accession number: O56263
Secondary accession number(s): Q778E8, Q9YX77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: January 24, 2006
Last modified: October 29, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Average number present in a viral particle is estimated to be 130-200 molecules.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3