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O56263

- NEP_I97A1

UniProt

O56263 - NEP_I97A1

Protein

Nuclear export protein

Gene

NS

Organism
Influenza A virus (strain A/Hong Kong/156/1997 H5N1 genotype Gs/Gd)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
  1. Functioni

    Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs to which the M1 protein is bound. Because the M1 protein is not available in large quantities until the later stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the nucleus before sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enters the cytoplasm only when they have associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced By similarity.By similarity

    GO - Biological processi

    1. RNA export from nucleus Source: InterPro
    2. viral process Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear export protein
    Short name:
    NEP
    Alternative name(s):
    Non-structural protein 2
    Short name:
    NS2
    Gene namesi
    Name:NS
    OrganismiInfluenza A virus (strain A/Hong Kong/156/1997 H5N1 genotype Gs/Gd)
    Taxonomic identifieri130763 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
    Homo sapiens (Human) [TaxID: 9606]
    Panthera pardus (Leopard) (Felis pardus) [TaxID: 9691]
    Panthera tigris (Tiger) [TaxID: 9694]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000008587: Genome

    Subcellular locationi

    Virion Curated. Host nucleus By similarity

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell
    2. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 121121Nuclear export proteinPRO_0000078991Add
    BLAST

    Interactioni

    Subunit structurei

    Binds M1 protein. May interact with human nucleoporin RAB/HRB and exportin XPO1/CRM1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO56263.
    SMRiO56263. Positions 63-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi12 – 2110Nuclear export signalBy similarity
    Motifi85 – 9410Nuclear export signal

    Sequence similaritiesi

    Belongs to the influenza viruses NEP family.Curated

    Family and domain databases

    InterProiIPR000968. Flu_NS2.
    [Graphical view]
    PfamiPF00601. Flu_NS2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform NEP (identifier: O56263-1) [UniParc]FASTAAdd to Basket

    Also known as: NS2

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDSNTVSSFQ DILKRMSKMQ LGSSSEDLNG MITQFESLKL YRDSLGEAVM    50
    RMGDLHSLQN RNGKWREQLS QKFEEIRWLI EEVRHRLRIT ENSFEQITFM 100
    QALQLLLEVE QEIRTFSFQL I 121
    Length:121
    Mass (Da):14,390
    Last modified:January 24, 2006 - v2
    Checksum:iA5381BD935178D31
    GO
    Isoform NS1 (identifier: O56264-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry O56264.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:230
    Mass (Da):26,237
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF036360 Genomic RNA. Translation: AAC34269.2.
    AF046091 Genomic RNA. Translation: AAC32093.1.
    AJ404736 Genomic RNA. Translation: CAC04090.1.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF036360 Genomic RNA. Translation: AAC34269.2 .
    AF046091 Genomic RNA. Translation: AAC32093.1 .
    AJ404736 Genomic RNA. Translation: CAC04090.1 .

    3D structure databases

    ProteinModelPortali O56263.
    SMRi O56263. Positions 63-116.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR000968. Flu_NS2.
    [Graphical view ]
    Pfami PF00601. Flu_NS2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness."
      Subbarao K., Klimov A., Katz J., Regnery H., Lim W., Hall H., Perdue M., Swayne D., Bender C., Huang J., Hemphill M., Rowe T., Shaw M., Xu X., Fukuda K., Cox N.
      Science 279:393-396(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. Cited for: SEQUENCE REVISION.
    3. "Comparisons of highly virulent H5N1 influenza A viruses isolated from humans and chickens from Hong Kong."
      Suarez D.L., Perdue M.L., Cox N., Rowe T., Bender C., Huang J., Swayne D.E.
      J. Virol. 72:6678-6688(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    4. "Avian-to-human transmission of H9N2 subtype influenza A viruses: relationship between H9N2 and H5N1 human isolates."
      Lin Y.P., Shaw M., Gregory V., Cameron K., Lim W., Klimov A., Subbarao K., Guan Y., Krauss S., Shortridge K., Webster R., Cox N., Hay A.
      Proc. Natl. Acad. Sci. U.S.A. 97:9654-9658(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiNEP_I97A1
    AccessioniPrimary (citable) accession number: O56263
    Secondary accession number(s): Q778E8, Q9YX77
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Average number present in a viral particle is estimated to be 130-200 molecules.

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3