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O55716

- RIR1_IIV6

UniProt

O55716 - RIR1_IIV6

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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene
IIV6-085L
Organism
Invertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei68 – 681Substrate By similarity
Sitei84 – 841Important for hydrogen atom transfer By similarity
Sitei91 – 911Allosteric effector binding By similarity
Binding sitei112 – 1121Substrate; via amide nitrogen By similarity
Sitei120 – 1201Allosteric effector binding By similarity
Active sitei270 – 2701Proton acceptor By similarity
Active sitei611 – 6111Cysteine radical intermediate By similarity
Active sitei613 – 6131Proton acceptor By similarity
Sitei626 – 6261Important for hydrogen atom transfer By similarity
Sitei890 – 8901Important for electron transfer By similarity
Sitei891 – 8911Important for electron transfer By similarity
Sitei955 – 9551Interacts with thioredoxin/glutaredoxin By similarity
Sitei958 – 9581Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. endonuclease activity Source: InterPro
  3. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
  2. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Cleaved into the following chain:
Gene namesi
ORF Names:IIV6-085L
OrganismiInvertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus)
Taxonomic identifieri176652 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageIridoviridaeIridovirus
Virus hostiAcheta domesticus (House cricket) [TaxID: 6997]
Chilo suppressalis (striped riceborer) [TaxID: 168631]
Gryllus bimaculatus (Two-spotted cricket) [TaxID: 6999]
Gryllus campestris [TaxID: 58607]
Spodoptera frugiperda (Fall armyworm) [TaxID: 7108]
ProteomesiUP000001359: Genome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Ribonucleoside-diphosphate reductase large subunit, 1st partPRO_0000376951Add
BLAST
Chaini272 – 611340IIV-6 RIR1 intein Reviewed predictionPRO_0000376952Add
BLAST
Chaini612 – 959348Ribonucleoside-diphosphate reductase large subunit, 2nd partPRO_0000377536Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi84 ↔ 626Redox-active By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Protein splicing

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Structurei

3D structure databases

ProteinModelPortaliO55716.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini378 – 508131DOD-type homing endonucleaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni83 – 842Substrate binding By similarity
Regioni751 – 7555Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF14528. LAGLIDADG_3. 1 hit.
PF02867. Ribonuc_red_lgC. 3 hits.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF48168. SSF48168. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55716-1 [UniParc]FASTAAdd to Basket

« Hide

MSDIIIDLSR DSKFDELGLK RLRESYMMRE ETSPQERFAY VCKQVGTDRD    50
HSQRLYEYTS KHWLSLSTPI LSFGKANHGL PISCYLSWIE DTKEGLIDTL 100
SEVNQLSMLG GGVGVGVGIR TSDNKSTGVM SHLNTYDACS LAYKQDGVRR 150
GSYAMYLNNN HPDVLQFIEM RKPTGDHNIR CLNLHHGLNI SDEFMELIEK 200
CDGGGNIDDT WNLIDPHTKK ITTVGARDLW QRILETRMKT GEPYICFIDT 250
CNKHMYDFQK KKGLTIKQSN LCVAPETMIL TEDGQFPIKD LEGKIIKVWN 300
GNEFSSVTVV KTGTEKELLE VELSNGCTLS CTPEHKFIIV KSYTEAKKQK 350
TDDNAIANAE RVDAQDLKPR MKLIKFDLPT LFGNSEHDIK YPYTHGFFCG 400
DGTYTKYGKP QLSLYGDKKE LLTYLDVRTM TGLEDASGRL NTWLPLDLAP 450
KFDVPINSSL ECRMEWLAGY LDADGCVFRN GTNESIQVSC IHLDFLKRIQ 500
LLLIGMGVTS KITKLHDEKI TTMPDGKGGQ KPYSCKPIWR LFISSSGLYH 550
LSEQGFETRR LKWEPRQPQR NAERFVEVLK VNKTGRVDDT YCFTEPINHA 600
GVFNGILTGQ CSEIILPTDS TRTAVCCLSS LNLEYYDEWK DNDLFIKDVM 650
EMLDNALTIF IEKAPPTISR AVNSAKKERS IGIGVLGFHS FLQQKNISFE 700
SDEAAKLNID IFTKLRSKID TFNLVLGSLR GSPEDAEGTG RRFCCTMAVA 750
PTATSSIIMG NTSPSVEPFR ANAYRQDTLS GSFLNKNRYL SRILSQRLNV 800
KEINEVWSNI VSNGGSVQQL PNNLLSEQEK QVFKTAFEIN QKWVIKHAAD 850
RQKYIDQSQS INLFLKPDIH KRELHSLHLN AWKSGLKTLY YLRSEKIADA 900
DKISSNHMIN SINFTNIKES IKDSIKVSIL EVRNKEKNYE EKICKLTNGR 950
RLSGCFACE 959
Length:959
Mass (Da):108,770
Last modified:June 1, 1998 - v1
Checksum:iCA051A57E685F419
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF303741 Genomic DNA. Translation: AAB94427.1.
PIRiT03053.
RefSeqiNP_149548.1. NC_003038.1.

Genome annotation databases

GeneIDi1733256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF303741 Genomic DNA. Translation: AAB94427.1 .
PIRi T03053.
RefSeqi NP_149548.1. NC_003038.1.

3D structure databases

ProteinModelPortali O55716.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1733256.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

Gene3Di 2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
InterProi IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF14528. LAGLIDADG_3. 1 hit.
PF02867. Ribonuc_red_lgC. 3 hits.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF48168. SSF48168. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR01445. intein_Nterm. 1 hit.
PROSITEi PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the first complete DNA sequence of an invertebrate iridovirus: coding strategy of the genome of Chilo iridescent virus."
    Jakob N.J., Mueller K., Bahr U., Darai G.
    Virology 286:182-196(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Comparative genomic analysis of the family Iridoviridae: re-annotating and defining the core set of iridovirus genes."
    Eaton H.E., Metcalf J., Penny E., Tcherepanov V., Upton C., Brunetti C.R.
    Virol. J. 4:11-11(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.

Entry informationi

Entry nameiRIR1_IIV6
AccessioniPrimary (citable) accession number: O55716
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: June 1, 1998
Last modified: June 11, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3