Reviewed, UniProtKB/Swiss-Prot O55716 (RIR1_IIV6)

Names and origin

Protein names

Recommended name:
    Ribonucleoside-diphosphate reductase large subunit
    EC=1.17.4.1
Alternative name(s):
    Ribonucleotide reductase large subunit
Cleaved into the following chain:
    1- Recommended name:
            IIV-6 RIR1 intein

Gene names

ORF Names:

IIV6-085L

Organism

Invertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus)

Taxonomic identifier

176652 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Iridoviridae › Iridovirus

Virus host

Acheta domesticus (House cricket) [TaxID: 6997]
Gryllus bimaculatus (Two-spotted cricket) [TaxID: 6999]
Spodoptera frugiperda (Fall armyworm) [TaxID: 7108]
Gryllus campestris [TaxID: 58607]
Chilo suppressalis (striped riceborer) [TaxID: 168631]

Protein attributes

Sequence length	959 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterodimer of a large and a small chain By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	DNA replication
Molecular function	Oxidoreductase
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Virus reference strain
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from electronic annotation. Source: InterPro ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Ribonucleoside-diphosphate reductase large subunit, 1st part		PRO_0000376951
	Chain	272 – 611	340	IIV-6 RIR1 intein Potential		PRO_0000376952
	Chain	612 – 959	348	Ribonucleoside-diphosphate reductase large subunit, 2nd part		PRO_0000377536
Regions
	Domain	378 – 508	131	DOD-type homing endonuclease
Sites
	Active site	84	1	Hydrogen atom transfer By similarity
	Active site	270	1	Proton acceptor By similarity
	Active site	611	1	Proton acceptor By similarity
	Active site	613	1	Proton acceptor By similarity
	Active site	626	1	Hydrogen atom transfer By similarity
	Active site	890	1	Electron transfer By similarity
	Active site	891	1	Electron transfer By similarity
	Site	91	1	Allosteric effector binding By similarity
	Site	120	1	Allosteric effector binding By similarity
	Site	955	1	Interacts with thioredoxin/glutaredoxin By similarity
	Site	958	1	Interacts with thioredoxin/glutaredoxin By similarity

Sequences

	Sequence		Length	Mass (Da)
	O55716-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: CA051A57E685F419	FASTA	959	108,770
	10 20 30 40 50 60 MSDIIIDLSR DSKFDELGLK RLRESYMMRE ETSPQERFAY VCKQVGTDRD HSQRLYEYTS 70 80 90 100 110 120 KHWLSLSTPI LSFGKANHGL PISCYLSWIE DTKEGLIDTL SEVNQLSMLG GGVGVGVGIR 130 140 150 160 170 180 TSDNKSTGVM SHLNTYDACS LAYKQDGVRR GSYAMYLNNN HPDVLQFIEM RKPTGDHNIR 190 200 210 220 230 240 CLNLHHGLNI SDEFMELIEK CDGGGNIDDT WNLIDPHTKK ITTVGARDLW QRILETRMKT 250 260 270 280 290 300 GEPYICFIDT CNKHMYDFQK KKGLTIKQSN LCVAPETMIL TEDGQFPIKD LEGKIIKVWN 310 320 330 340 350 360 GNEFSSVTVV KTGTEKELLE VELSNGCTLS CTPEHKFIIV KSYTEAKKQK TDDNAIANAE 370 380 390 400 410 420 RVDAQDLKPR MKLIKFDLPT LFGNSEHDIK YPYTHGFFCG DGTYTKYGKP QLSLYGDKKE 430 440 450 460 470 480 LLTYLDVRTM TGLEDASGRL NTWLPLDLAP KFDVPINSSL ECRMEWLAGY LDADGCVFRN 490 500 510 520 530 540 GTNESIQVSC IHLDFLKRIQ LLLIGMGVTS KITKLHDEKI TTMPDGKGGQ KPYSCKPIWR 550 560 570 580 590 600 LFISSSGLYH LSEQGFETRR LKWEPRQPQR NAERFVEVLK VNKTGRVDDT YCFTEPINHA 610 620 630 640 650 660 GVFNGILTGQ CSEIILPTDS TRTAVCCLSS LNLEYYDEWK DNDLFIKDVM EMLDNALTIF 670 680 690 700 710 720 IEKAPPTISR AVNSAKKERS IGIGVLGFHS FLQQKNISFE SDEAAKLNID IFTKLRSKID 730 740 750 760 770 780 TFNLVLGSLR GSPEDAEGTG RRFCCTMAVA PTATSSIIMG NTSPSVEPFR ANAYRQDTLS 790 800 810 820 830 840 GSFLNKNRYL SRILSQRLNV KEINEVWSNI VSNGGSVQQL PNNLLSEQEK QVFKTAFEIN 850 860 870 880 890 900 QKWVIKHAAD RQKYIDQSQS INLFLKPDIH KRELHSLHLN AWKSGLKTLY YLRSEKIADA 910 920 930 940 950 DKISSNHMIN SINFTNIKES IKDSIKVSIL EVRNKEKNYE EKICKLTNGR RLSGCFACE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the first complete DNA sequence of an invertebrate iridovirus: coding strategy of the genome of Chilo iridescent virus." Jakob N.J., Mueller K., Bahr U., Darai G. Virology 286:182-196(2001) [PubMed: 11448171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Comparative genomic analysis of the family Iridoviridae: re-annotating and defining the core set of iridovirus genes." Eaton H.E., Metcalf J., Penny E., Tcherepanov V., Upton C., Brunetti C.R. Virol. J. 4:11-11(2007) [PubMed: 17239238] [Abstract] Cited for: GENOME REANNOTATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF303741 Genomic DNA. Translation: AAB94427.1.
PIR	T03053.
RefSeq	NP_149548.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1733256.
Family and domain databases
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR013509. Ribncl_Rdtase_lsu_N. IPR000788. Ribncl_red_lg_C. [Graphical view]
PANTHER	PTHR11573. Ribncl_red_lg_C. 1 hit.
Pfam	PF02867. Ribonuc_red_lgC. 4 hits. PF00317. Ribonuc_red_lgN. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. False negative. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS00089. RIBORED_LARGE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR1_IIV6

Accession

Primary (citable) accession number: O55716

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	June 1, 1998
Last modified:	July 28, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)