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O55716

- RIR1_IIV6

UniProt

O55716 - RIR1_IIV6

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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

IIV6-085L

Organism
Invertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei68 – 681SubstrateBy similarity
Sitei84 – 841Important for hydrogen atom transferBy similarity
Sitei91 – 911Allosteric effector bindingBy similarity
Binding sitei112 – 1121Substrate; via amide nitrogenBy similarity
Sitei120 – 1201Allosteric effector bindingBy similarity
Active sitei270 – 2701Proton acceptorBy similarity
Active sitei611 – 6111Cysteine radical intermediateBy similarity
Active sitei613 – 6131Proton acceptorBy similarity
Sitei626 – 6261Important for hydrogen atom transferBy similarity
Sitei890 – 8901Important for electron transferBy similarity
Sitei891 – 8911Important for electron transferBy similarity
Sitei955 – 9551Interacts with thioredoxin/glutaredoxinBy similarity
Sitei958 – 9581Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. endonuclease activity Source: InterPro
  3. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
  2. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Cleaved into the following chain:
Gene namesi
ORF Names:IIV6-085L
OrganismiInvertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus)
Taxonomic identifieri176652 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageIridoviridaeIridovirus
Virus hostiAcheta domesticus (House cricket) [TaxID: 6997]
Chilo suppressalis (striped riceborer) [TaxID: 168631]
Gryllus bimaculatus (Two-spotted cricket) [TaxID: 6999]
Gryllus campestris [TaxID: 58607]
Spodoptera frugiperda (Fall armyworm) [TaxID: 7108]
ProteomesiUP000001359: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Ribonucleoside-diphosphate reductase large subunit, 1st partPRO_0000376951Add
BLAST
Chaini272 – 611340IIV-6 RIR1 inteinSequence AnalysisPRO_0000376952Add
BLAST
Chaini612 – 959348Ribonucleoside-diphosphate reductase large subunit, 2nd partPRO_0000377536Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi84 ↔ 626Redox-activeBy similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Protein splicing

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO55716.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini378 – 508131DOD-type homing endonucleasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni83 – 842Substrate bindingBy similarity
Regioni751 – 7555Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF14528. LAGLIDADG_3. 1 hit.
PF02867. Ribonuc_red_lgC. 3 hits.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF48168. SSF48168. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55716-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDIIIDLSR DSKFDELGLK RLRESYMMRE ETSPQERFAY VCKQVGTDRD
60 70 80 90 100
HSQRLYEYTS KHWLSLSTPI LSFGKANHGL PISCYLSWIE DTKEGLIDTL
110 120 130 140 150
SEVNQLSMLG GGVGVGVGIR TSDNKSTGVM SHLNTYDACS LAYKQDGVRR
160 170 180 190 200
GSYAMYLNNN HPDVLQFIEM RKPTGDHNIR CLNLHHGLNI SDEFMELIEK
210 220 230 240 250
CDGGGNIDDT WNLIDPHTKK ITTVGARDLW QRILETRMKT GEPYICFIDT
260 270 280 290 300
CNKHMYDFQK KKGLTIKQSN LCVAPETMIL TEDGQFPIKD LEGKIIKVWN
310 320 330 340 350
GNEFSSVTVV KTGTEKELLE VELSNGCTLS CTPEHKFIIV KSYTEAKKQK
360 370 380 390 400
TDDNAIANAE RVDAQDLKPR MKLIKFDLPT LFGNSEHDIK YPYTHGFFCG
410 420 430 440 450
DGTYTKYGKP QLSLYGDKKE LLTYLDVRTM TGLEDASGRL NTWLPLDLAP
460 470 480 490 500
KFDVPINSSL ECRMEWLAGY LDADGCVFRN GTNESIQVSC IHLDFLKRIQ
510 520 530 540 550
LLLIGMGVTS KITKLHDEKI TTMPDGKGGQ KPYSCKPIWR LFISSSGLYH
560 570 580 590 600
LSEQGFETRR LKWEPRQPQR NAERFVEVLK VNKTGRVDDT YCFTEPINHA
610 620 630 640 650
GVFNGILTGQ CSEIILPTDS TRTAVCCLSS LNLEYYDEWK DNDLFIKDVM
660 670 680 690 700
EMLDNALTIF IEKAPPTISR AVNSAKKERS IGIGVLGFHS FLQQKNISFE
710 720 730 740 750
SDEAAKLNID IFTKLRSKID TFNLVLGSLR GSPEDAEGTG RRFCCTMAVA
760 770 780 790 800
PTATSSIIMG NTSPSVEPFR ANAYRQDTLS GSFLNKNRYL SRILSQRLNV
810 820 830 840 850
KEINEVWSNI VSNGGSVQQL PNNLLSEQEK QVFKTAFEIN QKWVIKHAAD
860 870 880 890 900
RQKYIDQSQS INLFLKPDIH KRELHSLHLN AWKSGLKTLY YLRSEKIADA
910 920 930 940 950
DKISSNHMIN SINFTNIKES IKDSIKVSIL EVRNKEKNYE EKICKLTNGR

RLSGCFACE
Length:959
Mass (Da):108,770
Last modified:June 1, 1998 - v1
Checksum:iCA051A57E685F419
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF303741 Genomic DNA. Translation: AAB94427.1.
PIRiT03053.
RefSeqiNP_149548.1. NC_003038.1.

Genome annotation databases

GeneIDi1733256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF303741 Genomic DNA. Translation: AAB94427.1 .
PIRi T03053.
RefSeqi NP_149548.1. NC_003038.1.

3D structure databases

ProteinModelPortali O55716.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1733256.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

Gene3Di 2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
InterProi IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF14528. LAGLIDADG_3. 1 hit.
PF02867. Ribonuc_red_lgC. 3 hits.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF48168. SSF48168. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR01445. intein_Nterm. 1 hit.
PROSITEi PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the first complete DNA sequence of an invertebrate iridovirus: coding strategy of the genome of Chilo iridescent virus."
    Jakob N.J., Mueller K., Bahr U., Darai G.
    Virology 286:182-196(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Comparative genomic analysis of the family Iridoviridae: re-annotating and defining the core set of iridovirus genes."
    Eaton H.E., Metcalf J., Penny E., Tcherepanov V., Upton C., Brunetti C.R.
    Virol. J. 4:11-11(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.

Entry informationi

Entry nameiRIR1_IIV6
AccessioniPrimary (citable) accession number: O55716
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3