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O55647

- GP_TSWV

UniProt

O55647 - GP_TSWV

Protein

Envelope glycoprotein

Gene

GP

Organism
Tomato spotted wilt virus (TSWV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei484 – 4852Cleavage; by host signal peptidaseBy similarity

    GO - Biological processi

    1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
    2. modulation by virus of host morphology or physiology Source: InterPro
    3. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Envelope glycoprotein
    Short name:
    GP
    Alternative name(s):
    M polyprotein
    Cleaved into the following 2 chains:
    Gene namesi
    Name:GP
    OrganismiTomato spotted wilt virus (TSWV)
    Taxonomic identifieri11613 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeTospovirus
    Virus hostiFrankliniella occidentalis (Western flower thrips) [TaxID: 133901]
    Scirtothrips dorsalis [TaxID: 163899]
    Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
    Thrips tabaci [TaxID: 161014]

    Subcellular locationi

    Chain Glycoprotein G1 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated. Host endoplasmic reticulum membrane Curated; Single-pass type I membrane protein Curated
    Note: Glycoprotein G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs By similarity.By similarity
    Chain Glycoprotein G2 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated
    Note: Glycoprotein G2 is retained in the Golgi complex and probably contains a Golgi retention signal.By similarity

    GO - Cellular componenti

    1. host cell endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. host cell Golgi membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3535Sequence AnalysisAdd
    BLAST
    Chaini36 – 11351100Envelope glycoproteinPRO_0000036856Add
    BLAST
    Chaini36 – 484449Glycoprotein G1Sequence AnalysisPRO_0000036857Add
    BLAST
    Chaini485 – 1135651Glycoprotein G2Sequence AnalysisPRO_0000036858Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi116 – 1161N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi210 – 2101N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi340 – 3401N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi588 – 5881N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi605 – 6051N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi980 – 9801N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2.By similarity
    Glycosylated. Glycosylation is essential for proper subcellular location By similarity.By similarity

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    G1 and G2 interacts with each other.By similarity

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini36 – 345310LumenalSequence AnalysisAdd
    BLAST
    Topological domaini367 – 484118CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini485 – 1067583LumenalSequence AnalysisAdd
    BLAST
    Topological domaini1089 – 113547CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei346 – 36621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1068 – 108821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi41 – 433Cell attachment siteSequence Analysis

    Domaini

    The cell attachment site present in these glycoproteins may help in the adhesion of virus to cells.

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR005167. Bunya_G1.
    IPR014414. M_poly_TospoV.
    [Graphical view]
    PfamiPF03557. Bunya_G1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003960. M_poly_TospoV. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O55647-1 [UniParc]FASTAAdd to Basket

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    MRILKLLELV VKVSLFTIAL SSVLLAFLIF RATDAKVEII RGDHPEVYDD     50
    SAENEVPTAA SIQRKAILET LTNLMLESQT PGTRQIREGE SIIPIFAESN 100
    TQKTISVSDL PNNCLNASSL KCEIKGVSTY NVYYQVENNG VIYSCVSDSA 150
    EGLEKCDNSL NLPKRFSKVP VIPITKLDNK RHFSVGTKFF ISESLTQDNY 200
    PITYNSYPTN GTVSLQTVKL SGDCKITKSN FANPYTVSIT SPEKIMGYLI 250
    KKPGENVEHK VISFSGSASI TFTEEMLDGE HNLLCGDKSA KIPKTNKRVR 300
    DCIIKYSKSI YKQTAGINFS WIRLILIALL IYFPIRWLVN KTTKPLFLWY 350
    DLIGLITYPI LLLINCLWKY FPFKCSNCGN LCIITHECTK ICICNKSKAS 400
    KEHSSECPIL SKETDHGYNK HKWTSMEWFH LIVNTKLSFS LLKFVTEILI 450
    GLIILSQMPM SMAQTTQCLS GCFYVPGCPF LVTSKFEKCP EKDQCYCNVK 500
    EDKIIESIFG TNIVIEGPND CIENQNCAAH PSIDNLIKCR LGCEYLDLFR 550
    NKPLYNGFSD YTGSSLGLTS VGLYEAKRLR NGIIDSYNRT DKISGMIAGD 600
    SLNKNETSIP ENILPRQSLI FDSVVDGKYR YMIEQSLLGG GGTIFMLNDK 650
    TSETAKKFVI YIKSVGIHYE VSEKYTTAPI QSTHTDFYST CTGNCDTCRK 700
    NQALTGFQDF CITPTSYWGC EEAWCFAINE GATCGFCRNI YDMDKSYRIY 750
    SVLKSTIVAD VCISGILGGQ CSKITEEVPY ENALFQADIQ ADLHNDGITI 800
    GELIAHGPDS HIYSGNIANL NDPVKMFGHP QLTHDGVPIF TKKTLEGDDM 850
    SWDCAAIGKK SITIKTCGYD TYRFRSGLEQ ISDIPVSFKD FSSFFLEKAF 900
    SLGKLKIVVD LPSDLFKVAP KRPSITSTSL NCNGCLLCGQ GLSCILEFFS 950
    DLTFSTAISI DACSLSTYQL AVKKGSNKYN ITMFCSANPD KKKMTLYPEG 1000
    NPDISVEVLV NNVIVEEPEN IIDQNDEYAH EEQQYNSDSS AWGFWDYIKS 1050
    PFNFIASYFG SFFDTIRVIL LIAFIFLVIY FCSILTTICK GYVKNESYKS 1100
    RSKIEDDDDS EIKAPMLMKD TMTRRRPPMD FSHLV 1135
    Length:1,135
    Mass (Da):127,333
    Last modified:June 1, 1998 - v1
    Checksum:iBD9977103BE957F2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB010996 Genomic RNA. Translation: BAA24894.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB010996 Genomic RNA. Translation: BAA24894.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR005167. Bunya_G1.
    IPR014414. M_poly_TospoV.
    [Graphical view ]
    Pfami PF03557. Bunya_G1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003960. M_poly_TospoV. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis for M RNA segment of tomato spotted wilt tospovirus isolated in Japan."
      Ohnishi J., Kirita M., Hosokawa D., Tsuda S.
      Nihon Shokubutsu Byori Gakkaiho 63:277-277(1997)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiGP_TSWV
    AccessioniPrimary (citable) accession number: O55647
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3