O55527 (C_SENDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein C'

Cleaved into the following 2 chains:

1. Protein C
2. Protein Y1

Gene names

Name:

P/V/C

Organism

Sendai virus (strain Ohita) (SeV) [Reference proteome]

Taxonomic identifier

302272 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Respirovirus › Sendai virus

Virus host

Cavia cutleri (Guinea pig) [TaxID: 10144]
Cricetidae sp. (Hamster) [TaxID: 36483]
Mus musculus (Mouse) [TaxID: 10090]
Rattus norvegicus (Rat) [TaxID: 10116]

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The different products prevent the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways. They inhibit IFN-alpha/beta induced tyrosine phosphorylation of STAT1 and STAT2. Blocking the IFN-alpha/beta pathway requires binding to STAT1 in the cytoplasm. They inhibit IFN-gamma induced serine phosphorylation of STAT1. Block the IFN-gamma pathway in a STAT1-binding independent mechanism, preventing the GAF (tyrosine-phosphorylated STAT1 dimer) to bind its promoter in the nucleus. May also have a role in preventing the cell to enter apoptosis. Modulate regulation of viral transcription and replication. Overexpression inhibits the viral RNA polymerase. The absence of all C', C and Y1 proteins leads to viral delayed growth. Plays an important role in virion particles release. Modulates virion shape By similarity.
Subunit structure	The different products bind the N-terminal part of unphosphorylated and phosphorylated human STAT1, preventing the formation of STAT1 homodimers and STAT1/STAT2 heterodimers By similarity. The binding of C protein to L protein has an inhibitory effect on viral transcription and replication By similarity.
Subcellular location	Host cytoplasm By similarity. Note: Protein C' seems to localize around the Golgi By similarity.
Post-translational modification	Protein Y1 is produced not only by alternative initiation, but also by preoteolytic cleavage of C'. Only alternative initiation is detected in vitro, whereas in vivo cleavage seems to be predominant By similarity.
Miscellaneous	The C protein is found in virion at a ratio of approximately 40 molecules per virion, presumably associated with the nucleocapsid By similarity. The P/V/C gene has two overlapping open reading frames. One encodes the P/V/W proteins and the other the C/Y proteins.
Sequence similarities	Belongs to the respirovirus protein C family.
Caution	The C' protein uses an unusual ACG start codon.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host STAT1 by virus Inhibition of host STAT2 by virus Inhibition of host innate immune response by virus Inhibition of host interferon signaling pathway by virus Interferon antiviral system evasion Viral immunoevasion
Cellular component	Host cytoplasm
Coding sequence diversity	Alternative initiation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	suppression by virus of host STAT1 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host STAT2 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative initiation. [Align] [Select]

Isoform C' (identifier: O55527-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: The initiator methionine is coded by an unusual start codon ACG.

Isoform C (identifier: O55527-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: Missing.

Note: Most abundant isoform in infected cells.

Isoform Y1 (identifier: O55527-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed; by host By similarity
	Chain	2 – 215	214	Protein C'		PRO_0000039408
Natural variations
	Alternative sequence	1 – 34	34	Missing in isoform Y1.		VSP_018943
	Alternative sequence	1 – 11	11	Missing in isoform C.		VSP_018942
	Natural variant	181	1	F → S in strain: Isolate MVC11; avirulent mutant.

Sequences

	Sequence		Length	Mass (Da)
	Isoform C' [UniParc]. Last modified January 23, 2007. Version 3. Checksum: A94696F9D9875685	FASTA	215	25,162
	10 20 30 40 50 60 MASATLPAWI KMPSFLKKIL KLRGRRQEDE SRSRMLSDSS TQSYQVNQLT SEGTEAGSTI 70 80 90 100 110 120 PSTPSKGQAL PTESKVRARE KSRHRRPKII DQVRRVESLG EQASQRQKHM LETLINKIYT 130 140 150 160 170 180 GPLGEELVQT LYLRIWAMEE TPESLKILQM REDIRDQVLK MKTERWLRTL IRGEKTKLKD 190 200 210 FQKRYEEVHP YLMKEKVEQI IMEEAWSLAA HIVQE
	Isoform C [UniParc]. Checksum: 950DD3DA387229C9	FASTA	204	23,992
	10 20 30 40 50 60 MPSFLKKILK LRGRRQEDES RSRMLSDSST QSYQVNQLTS EGTEAGSTIP STPSKGQALP 70 80 90 100 110 120 TESKVRAREK SRHRRPKIID QVRRVESLGE QASQRQKHML ETLINKIYTG PLGEELVQTL 130 140 150 160 170 180 YLRIWAMEET PESLKILQMR EDIRDQVLKM KTERWLRTLI RGEKTKLKDF QKRYEEVHPY 190 200 LMKEKVEQII MEEAWSLAAH IVQE
	Isoform Y1 [UniParc]. Checksum: A250A361AF313179	FASTA	181	21,178
	10 20 30 40 50 60 MLSDSSTQSY QVNQLTSEGT EAGSTIPSTP SKGQALPTES KVRAREKSRH RRPKIIDQVR 70 80 90 100 110 120 RVESLGEQAS QRQKHMLETL INKIYTGPLG EELVQTLYLR IWAMEETPES LKILQMREDI 130 140 150 160 170 180 RDQVLKMKTE RWLRTLIRGE KTKLKDFQKR YEEVHPYLMK EKVEQIIMEE AWSLAAHIVQ E

References

[1]

"Isolation of an avirulent mutant of Sendai virus with two amino acid mutations from a highly virulent field strain through adaptation to LLC-MK2 cells." Itoh M., Isegawa Y., Hotta H., Homma M. J. Gen. Virol. 78:3207-3215(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate MVC11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB005795 Genomic RNA. Translation: BAA24385.1. AB005795 Genomic RNA. Translation: BAA24387.1. AB005795 Genomic RNA. Translation: BAA24388.1. AB005796 Genomic RNA. Translation: BAA24394.1. AB005796 Genomic RNA. Translation: BAA24396.1. AB005796 Genomic RNA. Translation: BAA24397.1.
RefSeq	NP_056872.1. NC_001552.1. NP_056874.1. NC_001552.1. NP_056875.1. NC_001552.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1489773. 1489774. 1489781.
Family and domain databases
InterPro	IPR002608. Paramyxo_C. [Graphical view]
Pfam	PF01692. Paramyxo_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C_SENDO

Accession

Primary (citable) accession number: O55527
Secondary accession number(s): O55529 , O92615, Q9WME0, Q9WME1, Q9WME2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 48 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program