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Protein

Sigma non-opioid intracellular receptor 1

Gene

Sigmar1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane (PubMed:12730355). Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration (PubMed:11149946, PubMed:14622179, PubMed:15571673, PubMed:15777781, PubMed:23332758, PubMed:9425306, PubMed:9603192). Necessary for proper mitochondrial axonal transport in motor neurons, in particular the retrograde movement of mitochondria (PubMed:25678561).9 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei126Important for ligand bindingBy similarity1
Sitei172Important for ligand bindingBy similarity1

GO - Molecular functioni

  • opioid receptor activity Source: Ensembl
  • receptor activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Lipid transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Sigma non-opioid intracellular receptor 1
Alternative name(s):
Sigma 1-type opioid receptor
Short name:
Sigma1-receptor
Short name:
Sigma1R
Gene namesi
Name:Sigmar1
Synonyms:Oprs1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1195268. Sigmar1.

Subcellular locationi

  • Nucleus inner membrane By similarity
  • Nucleus outer membrane By similarity
  • Nucleus envelope 1 Publication
  • Cytoplasmic vesicle By similarity
  • Endoplasmic reticulum membrane 1 Publication
  • Membrane By similarity; Single-pass membrane protein By similarity
  • Lipid droplet 1 Publication
  • Cell junction By similarity
  • Cell membrane By similarity
  • Cell projectiongrowth cone By similarity
  • Cell junctionsynapsepostsynaptic cell membranepostsynaptic density 1 Publication

  • Note: During interphase, detected at the inner and outer nuclear membrane and the endoplasmic reticulum. Detected on cytoplasmic vesicles during mitosis (By similarity). Targeted to lipid droplets, cholesterol and galactosylceramide-enriched domains of the endoplasmic reticulum (PubMed:12730355). Enriched at cell-cell communication regions, growth cone and postsynaptic structures. Localization is modulated by ligand-binding. In motor neurons it is enriched at cholinergic postsynaptic densities (PubMed:20167253).By similarity2 Publications

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9LumenalBy similarity9
Transmembranei10 – 30HelicalBy similarityAdd BLAST21
Topological domaini31 – 223CytoplasmicBy similarityAdd BLAST193

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasmic vesicle, Endoplasmic reticulum, Lipid droplet, Membrane, Nucleus, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Mice display decreased hypermotility response induced by (+)SKF-10047 challenge and reduced formalin-induced pain (PubMed:14622179). Mice display motor coordination defects, muscle weakness, partial neuromuscular junction innervation, and motor neuron degeneration (PubMed:20167253, PubMed:25678561).3 Publications

Chemistry databases

ChEMBLiCHEMBL3465.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002686531 – 223Sigma non-opioid intracellular receptor 1Add BLAST223

Proteomic databases

MaxQBiO55242.
PaxDbiO55242.
PeptideAtlasiO55242.
PRIDEiO55242.

PTM databases

PhosphoSitePlusiO55242.

Expressioni

Tissue specificityi

Widely expressed with higher expression in liver, brain, kidney and thymus. Expressed throughout the brain with higher expression within cerebral cortex, hippocampus and cerebellum. Within the hippocampus expressed in cornu ammonis pyramidal neurons, the granular cells of the dentate gyrus as well as interneurons. Within the cerebellum, expressed in Purkinje cell bodies (PubMed:11207432, PubMed:11476895, PubMed:11687279, PubMed:9603192). Highly expressed in the brainstem and motor neurons of the spinal cord (PubMed:20167253). Expressed by neural retina, retinal pigment epithelial cells and lens (PubMed:11207432, PubMed:11476895, PubMed:11687279, PubMed:9603192).5 Publications

Gene expression databases

BgeeiENSMUSG00000036078.
CleanExiMM_OPRS1.
ExpressionAtlasiO55242. baseline and differential.
GenevisibleiO55242. MM.

Interactioni

Subunit structurei

Homotrimer (By similarity). Interacts with KCNA4 (By similarity). Interacts with KCNA2; cocaine consumption leads to increased interaction (PubMed:23332758). Forms a ternary complex with ANK2 and ITPR3. The complex is disrupted by agonists (PubMed:11149946).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
I79_019946G3I8R93EBI-1557700,EBI-988311From a different organism.
Kcna2P631413EBI-1557700,EBI-644033

Protein-protein interaction databases

IntActiO55242. 2 interactors.
STRINGi10090.ENSMUSP00000056027.

Chemistry databases

BindingDBiO55242.

Structurei

3D structure databases

ProteinModelPortaliO55242.
SMRiO55242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 8Targeting to endoplasmic reticulum-associated lipid droplets1 Publication7
Regioni99 – 106Important for ligand-bindingBy similarity8
Regioni177 – 223C-terminal hydrophobic regionCuratedAdd BLAST47

Domaini

The C-terminal helices form a flat, hydrophobic surface that is probably tightly associated with the cytosolic surface of the endoplasmic reticulum membrane.By similarity

Sequence similaritiesi

Belongs to the ERG2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4143. Eukaryota.
ENOG410XP0Z. LUCA.
GeneTreeiENSGT00390000012082.
HOGENOMiHOG000211245.
HOVERGENiHBG058220.
InParanoidiO55242.
KOiK20719.
OMAiLFYTLRA.
OrthoDBiEOG091G0KD7.
PhylomeDBiO55242.
TreeFamiTF300106.

Family and domain databases

InterProiIPR006716. ERG2_sigma1_rcpt-like.
[Graphical view]
PfamiPF04622. ERG2_Sigma1R. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O55242-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPWAAGRRWA WITLILTIIA VLIQAAWLWL GTQNFVFSRE EIAQLARQYA
60 70 80 90 100
GLDHELAFSR LIVELRRLHP GHVLPDEELQ WVFVNAGGWM GAMCILHASL
110 120 130 140 150
SEYVLLFGTA LGSHGHSGRY WAEISDTIIS GTFHQWKEGT TKSEVFYPGE
160 170 180 190 200
TVVHGPGEAT ALEWGPNTWM VEYGRGVIPS TLFFALADTF FSTQDYLTLF
210 220
YTLRAYARGL RLELTTYLFG QDS
Length:223
Mass (Da):25,250
Last modified:June 1, 1998 - v1
Checksum:i54BB2F14472E3512
GO
Isoform 2 (identifier: O55242-2) [UniParc]FASTAAdd to basket
Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     118-148: Missing.

Show »
Length:192
Mass (Da):21,633
Checksum:i6C75160BF446D3BC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti192S → G in AAF64281 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021987118 – 148Missing in isoform 2. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030198 mRNA. Translation: AAC39951.1.
AF030199 Genomic DNA. Translation: AAB97683.1.
AF004927 mRNA. Translation: AAC33306.1.
AF226605 mRNA. Translation: AAF64281.1.
AK154300 mRNA. Translation: BAE32499.1.
AK159886 mRNA. Translation: BAE35455.1.
BC002000 mRNA. Translation: AAH02000.1.
BC019930 mRNA. Translation: AAH19930.1.
CCDSiCCDS18070.1. [O55242-1]
CCDS71362.1. [O55242-2]
PIRiJC5815.
RefSeqiNP_001273467.1. NM_001286538.1.
NP_001273468.1. NM_001286539.1. [O55242-2]
NP_001273469.1. NM_001286540.1.
NP_001273470.1. NM_001286541.1.
NP_001273480.1. NM_001286551.1.
NP_035144.1. NM_011014.3. [O55242-1]
XP_017175516.1. XM_017320027.1. [O55242-1]
UniGeneiMm.425181.

Genome annotation databases

EnsembliENSMUST00000059354; ENSMUSP00000056027; ENSMUSG00000036078. [O55242-1]
ENSMUST00000071561; ENSMUSP00000071492; ENSMUSG00000036078. [O55242-2]
GeneIDi18391.
KEGGimmu:18391.
UCSCiuc008sjk.2. mouse. [O55242-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030198 mRNA. Translation: AAC39951.1.
AF030199 Genomic DNA. Translation: AAB97683.1.
AF004927 mRNA. Translation: AAC33306.1.
AF226605 mRNA. Translation: AAF64281.1.
AK154300 mRNA. Translation: BAE32499.1.
AK159886 mRNA. Translation: BAE35455.1.
BC002000 mRNA. Translation: AAH02000.1.
BC019930 mRNA. Translation: AAH19930.1.
CCDSiCCDS18070.1. [O55242-1]
CCDS71362.1. [O55242-2]
PIRiJC5815.
RefSeqiNP_001273467.1. NM_001286538.1.
NP_001273468.1. NM_001286539.1. [O55242-2]
NP_001273469.1. NM_001286540.1.
NP_001273470.1. NM_001286541.1.
NP_001273480.1. NM_001286551.1.
NP_035144.1. NM_011014.3. [O55242-1]
XP_017175516.1. XM_017320027.1. [O55242-1]
UniGeneiMm.425181.

3D structure databases

ProteinModelPortaliO55242.
SMRiO55242.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO55242. 2 interactors.
STRINGi10090.ENSMUSP00000056027.

Chemistry databases

BindingDBiO55242.
ChEMBLiCHEMBL3465.

PTM databases

PhosphoSitePlusiO55242.

Proteomic databases

MaxQBiO55242.
PaxDbiO55242.
PeptideAtlasiO55242.
PRIDEiO55242.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059354; ENSMUSP00000056027; ENSMUSG00000036078. [O55242-1]
ENSMUST00000071561; ENSMUSP00000071492; ENSMUSG00000036078. [O55242-2]
GeneIDi18391.
KEGGimmu:18391.
UCSCiuc008sjk.2. mouse. [O55242-1]

Organism-specific databases

CTDi10280.
MGIiMGI:1195268. Sigmar1.

Phylogenomic databases

eggNOGiKOG4143. Eukaryota.
ENOG410XP0Z. LUCA.
GeneTreeiENSGT00390000012082.
HOGENOMiHOG000211245.
HOVERGENiHBG058220.
InParanoidiO55242.
KOiK20719.
OMAiLFYTLRA.
OrthoDBiEOG091G0KD7.
PhylomeDBiO55242.
TreeFamiTF300106.

Miscellaneous databases

ChiTaRSiSigmar1. mouse.
PROiO55242.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036078.
CleanExiMM_OPRS1.
ExpressionAtlasiO55242. baseline and differential.
GenevisibleiO55242. MM.

Family and domain databases

InterProiIPR006716. ERG2_sigma1_rcpt-like.
[Graphical view]
PfamiPF04622. ERG2_Sigma1R. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSGMR1_MOUSE
AccessioniPrimary (citable) accession number: O55242
Secondary accession number(s): Q9JKU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Depletion by RNAi enhances kappa-type opioid receptor-mediated analgesia and prevents the memory-improving effects of (-)- and (+)-pentazocine.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.