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O55241 (OREX_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Orexin
Alternative name(s):
Hypocretin
Short name=Hcrt

Cleaved into the following 2 chains:

  1. Orexin-A
    Alternative name(s):
    Hypocretin-1
    Short name=Hcrt1
  2. Orexin-B
    Alternative name(s):
    Hypocretin-2
    Short name=Hcrt2
Gene names
Name:Hcrt
Synonyms:Ox, Ppox
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length130 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.

Subcellular location

Rough endoplasmic reticulum By similarity. Cytoplasmic vesicle By similarity. Cell junctionsynapse By similarity. Note: Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses By similarity.

Tissue specificity

Restricted to neuronal cell bodies of the dorsal and lateral hypothalamus.

Post-translational modification

Specific enzymatic cleavages at paired basic residues yield the different active peptides.

Sequence similarities

Belongs to the orexin family.

Ontologies

Keywords
   Cellular componentCell junction
Cytoplasmic vesicle
Endoplasmic reticulum
Synapse
   DomainSignal
   Molecular functionNeuropeptide
   PTMAmidation
Cleavage on pair of basic residues
Disulfide bond
Pyrrolidone carboxylic acid
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processeating behavior

Inferred from electronic annotation. Source: Ensembl

negative regulation of DNA replication

Inferred from electronic annotation. Source: Ensembl

negative regulation of potassium ion transport

Inferred from electronic annotation. Source: Ensembl

negative regulation of transmission of nerve impulse

Inferred from electronic annotation. Source: Ensembl

neuropeptide signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

phospholipase C-activating G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of calcium ion transport

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration

Inferred from electronic annotation. Source: Ensembl

positive regulation of transmission of nerve impulse

Inferred from electronic annotation. Source: Ensembl

protein kinase C-activating G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

regulation of excitatory postsynaptic membrane potential

Inferred from electronic annotation. Source: Ensembl

regulation of neurotransmitter secretion

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

perinuclear region of cytoplasm

Inferred from direct assay PubMed 11748295. Source: MGI

rough endoplasmic reticulum

Inferred from electronic annotation. Source: UniProtKB-SubCell

secretory granule

Inferred from electronic annotation. Source: Ensembl

synapse

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 By similarity
Peptide33 – 6533Orexin-A
PRO_0000020264
Peptide69 – 9628Orexin-B
PRO_0000020265
Propeptide97 – 13034
PRO_0000020266

Amino acid modifications

Modified residue331Pyrrolidone carboxylic acid By similarity
Modified residue651Leucine amide By similarity
Modified residue961Methionine amide By similarity
Disulfide bond38 ↔ 44 By similarity
Disulfide bond39 ↔ 46 By similarity

Sequences

Sequence LengthMass (Da)Tools
O55241 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: D3C223F9EE835F1C

FASTA13013,503
        10         20         30         40         50         60 
MNFPSTKVPW AAVTLLLLLL LPPALLSLGV DAQPLPDCCR QKTCSCRLYE LLHGAGNHAA 

        70         80         90        100        110        120 
GILTLGKRRP GPPGLQGRLQ RLLQANGNHA AGILTMGRRA GAELEPHPCS GRGCPTVTTT 

       130 
ALAPRGGSGV 

« Hide

References

[1]"Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior."
Sakurai T., Amemiya A., Ishii M., Matsuzaki I., Chemelli R.M., Tanaka H., Williams S.C., Richardson J.A., Kozlowski G.P., Wilson S., Arch J.R.S., Buckingham R.E., Haynes A.C., Carr S.A., Annan R.S., McNulty D.E., Liu W.-S., Terrett J.A. expand/collapse author list , Elshourbagy N.A., Bergsma D.J., Yanagisawa M.
Cell 92:573-585(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The hypocretins: hypothalamus-specific peptides with neuroexcitatory activity."
de Lecea L., Kilduff T.S., Peyron C., Gao X.-B., Foye P.E., Danielson P.E., Fukuhara C., Battenberg E.L.F., Gautvik V.T., Bartlett F.S. II, Frankel W.N., van den Pol A.N., Bloom F.E., Gautvik K.M., Sutcliffe J.G.
Proc. Natl. Acad. Sci. U.S.A. 95:322-327(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6J.
[3]"Hypocretin/orexin, sleep and narcolepsy."
Hungs M., Mignot E.
Bioessays 23:397-408(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[4]"To eat or to sleep? Orexin in the regulation of feeding and wakefulness."
Willie J.T., Chemelli R.M., Sinton C.M., Yanagisawa M.
Annu. Rev. Neurosci. 24:429-458(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Web resources

Protein Spotlight

Qui dort dine - Issue 15 of October 2001

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF041242 mRNA. Translation: AAC40040.1.
AF019566 mRNA. Translation: AAC02934.1.
CCDSCCDS25436.1.
RefSeqNP_034540.1. NM_010410.2.
UniGeneMm.10096.

3D structure databases

ProteinModelPortalO55241.
SMRO55241. Positions 33-65, 71-96.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000057578.

Proteomic databases

PRIDEO55241.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000055083; ENSMUSP00000057578; ENSMUSG00000045471.
GeneID15171.
KEGGmmu:15171.
UCSCuc007lmg.1. mouse.

Organism-specific databases

CTD3060.
MGIMGI:1202306. Hcrt.

Phylogenomic databases

eggNOGNOG70925.
HOGENOMHOG000230990.
HOVERGENHBG000256.
InParanoidO55241.
KOK05246.
OMAELEPHPC.
PhylomeDBO55241.
TreeFamTF330756.

Gene expression databases

BgeeO55241.
CleanExMM_HCRT.
MM_PPOX.
GenevestigatorO55241.

Family and domain databases

InterProIPR001704. Orexin.
[Graphical view]
PANTHERPTHR15173. PTHR15173. 1 hit.
PfamPF02072. Orexin. 1 hit.
[Graphical view]
PIRSFPIRSF037824. Orexin. 1 hit.
PRINTSPR01091. OREXINPP.
ProtoNetSearch...

Other

NextBio287686.
PROO55241.
SOURCESearch...

Entry information

Entry nameOREX_MOUSE
AccessionPrimary (citable) accession number: O55241
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot