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O55240

- RDH1_MOUSE

UniProt

O55240 - RDH1_MOUSE

Protein

11-cis retinol dehydrogenase

Gene

Rdh5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Stereospecific 11-cis retinol dehydrogenase, which catalyzes the final step in the biosynthesis of 11-cis retinaldehyde, the universal chromophore of visual pigments. Also able to oxidize 9-cis-retinol and 13-cis-retinol, but not all-trans-retinol. Active in the presence of NAD as cofactor but not in the presence of NADP.2 Publications

    Catalytic activityi

    11-cis-retinol-[retinal-binding-protein] + NAD+ = 11-cis-retinal-[retinol-binding-protein] + NADH.2 Publications

    Enzyme regulationi

    Inhibited by 9-cis-, 13-cis- and all-trans-retinoic acids, with the most potent inhibitor being 13-cis-retinoic acid. Weakly inhibited by oleic acid.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei163 – 1631SubstrateBy similarity
    Active sitei175 – 1751Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi32 – 5625NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. oxidoreductase activity Source: UniProtKB-KW

    GO - Biological processi

    1. response to stimulus Source: UniProtKB-KW
    2. retinol metabolic process Source: UniProtKB-UniPathway
    3. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BRENDAi1.1.1.105. 244.
    ReactomeiREACT_188277. The canonical retinoid cycle in rods (twilight vision).
    UniPathwayiUPA00912.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    11-cis retinol dehydrogenaseImported (EC:1.1.1.315Imported2 Publications)
    Short name:
    11-cis RDHBy similarity
    Short name:
    11-cis RoDH1 Publication
    Alternative name(s):
    9-cis retinol dehydrogenaseImported
    Cis-retinol dehydrogenase1 Publication
    Retinol dehydrogenase 5Imported
    Gene namesi
    Name:Rdh5Imported
    Synonyms:cRDH1 Publication, rdhImported, RDH4Imported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:1201412. Rdh5.

    Subcellular locationi

    Membrane By similarity; Peripheral membrane protein By similarity. Endoplasmic reticulum lumen 1 Publication

    GO - Cellular componenti

    1. cell body Source: Ensembl
    2. endoplasmic reticulum lumen Source: MGI
    3. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mutant mice develop normally and are fertile. No abnormalities can be found in the retinal structure, rhodopsin content and fundus appearance of their eyes. Mice display a mild visual phenotype of impaired dark adaptation and accumulation of 11-cis- and 13-cis-retinols and 11-cis- and 13-cis-retinyl esters in the eyes.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 31829511-cis retinol dehydrogenaseSequence AnalysisPRO_0000419633Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi160 – 1601N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiO55240.

    Expressioni

    Tissue specificityi

    Expressed in eye, liver, kidney, brain, intestine, placenta, epididymus and submaxillary gland. In eye, strongly expressed in the retinal pigment epithelium, with lower expression levels detected in the inner segment of the photoreceptor cells and in the outer plexiform layer. In kidney, strong expression detected in the distal tubules and the transitional epithelium in the renal pelvis, with weaker expression detected in the epithelium of the outer stripe of the outer zone of the medulla. In liver, detected in hepatocytes in the centrilobular area. In lung, present in Clara cells in the epithelium of the bronchiole, in parenchyma and in cartilage surrounding the secondary bronchi. In skin, expressed in epidermis, hair follicles and mast cells in the dermis. Expressed in heart (PubMed:10588954 and PubMed:10739682). Not detected in heart (PubMed:9539749). Not detected in lung, spleen, skeletal muscle and testis.4 Publications

    Developmental stagei

    Abundantly expressed during embryonic development, especially in the developing central nervous system and sensory organs, cranial and spinal ganglia and endoderm of foregut and hindgut. At E10, detected along the entire neural tube, the mid- and hindbrain floor, the central canal of the brain vesicles, spinal cord, lung mesenchyme, the trabecular layer of the heart ventricles, endoderm and endodermally-derived structures such as tracheal epithelium and liver. At E11, expressed in the brain vesicles, along the spinal cord, myotome, migrating muscle progenitor cells in the body wall, cells of the genital ridge, spinal ganglion, liver, cerebellar primordium, basal cells of the neuroepithelium of the mesenchephalic flexure, collections of cells in the pons, Rathke's pouch, spinal and cranial ganglia and the floor plate, retina, lens, optic stalks and the neural crest-derived mesenchyme in the anterior eye segment. During eye development, expression restricted to the retinal pigment epithelium of the posterior hemisphere at E18, with expression levels increasing postnatally to P16.2 Publications

    Gene expression databases

    BgeeiO55240.
    GenevestigatoriO55240.

    Structurei

    3D structure databases

    ProteinModelPortaliO55240.
    SMRiO55240. Positions 25-284.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    GeneTreeiENSGT00620000087655.
    HOVERGENiHBG005482.
    InParanoidiO55240.
    KOiK00061.
    OMAiGPTPWLT.
    OrthoDBiEOG7FXZZX.
    PhylomeDBiO55240.
    TreeFamiTF325617.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O55240-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWLPLLLGAL LWAVLWLLRD RQSLPASDAF IFITGCDSGF GRLLALQLDQ    50
    KGFQVLAGCL TPSGAEDLQQ MASSRLHTTL LDITDPQNVQ QVAKWVKTRV 100
    GETGLFGLVN NAGVAGIIGP TPWLTQDDFQ RVLSVNTLGP IGVTLALLPL 150
    LQQARGRVVN ITSVLGRIAA NGGGYCVSKF GLEAFSDSLR RDMAPFGVQV 200
    SIVEPGFFRT PVTNLESLES TLKACWARLP PAIQAHYGEA FLDTYLRVQR 250
    RIMNLICDPE LTKVTSCLEH ALTARHPRTR YSPGWDAKLL WLPASYLPAR 300
    VVDAVLTWIL PRPAQSVS 318
    Length:318
    Mass (Da):34,826
    Last modified:June 1, 1998 - v1
    Checksum:iAA8DB76CD0BF7DFC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97752 Genomic DNA. Translation: CAA66347.1.
    AF013288 mRNA. Translation: AAC25951.1.
    AF033195 mRNA. Translation: AAC00491.1.
    AF033196 mRNA. Translation: AAC00492.1.
    AK135139 mRNA. Translation: BAE22435.1.
    AC122380 Genomic DNA. No translation available.
    CH466578 Genomic DNA. Translation: EDL24632.1.
    BC021372 mRNA. Translation: AAH21372.1.
    CCDSiCCDS24298.1.
    RefSeqiNP_598767.1. NM_134006.4.
    XP_006513450.1. XM_006513387.1.
    UniGeneiMm.439856.

    Genome annotation databases

    EnsembliENSMUST00000026406; ENSMUSP00000026406; ENSMUSG00000025350.
    GeneIDi19682.
    KEGGimmu:19682.
    UCSCiuc007how.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97752 Genomic DNA. Translation: CAA66347.1 .
    AF013288 mRNA. Translation: AAC25951.1 .
    AF033195 mRNA. Translation: AAC00491.1 .
    AF033196 mRNA. Translation: AAC00492.1 .
    AK135139 mRNA. Translation: BAE22435.1 .
    AC122380 Genomic DNA. No translation available.
    CH466578 Genomic DNA. Translation: EDL24632.1 .
    BC021372 mRNA. Translation: AAH21372.1 .
    CCDSi CCDS24298.1.
    RefSeqi NP_598767.1. NM_134006.4.
    XP_006513450.1. XM_006513387.1.
    UniGenei Mm.439856.

    3D structure databases

    ProteinModelPortali O55240.
    SMRi O55240. Positions 25-284.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O55240.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026406 ; ENSMUSP00000026406 ; ENSMUSG00000025350 .
    GeneIDi 19682.
    KEGGi mmu:19682.
    UCSCi uc007how.1. mouse.

    Organism-specific databases

    CTDi 5959.
    MGIi MGI:1201412. Rdh5.

    Phylogenomic databases

    GeneTreei ENSGT00620000087655.
    HOVERGENi HBG005482.
    InParanoidi O55240.
    KOi K00061.
    OMAi GPTPWLT.
    OrthoDBi EOG7FXZZX.
    PhylomeDBi O55240.
    TreeFami TF325617.

    Enzyme and pathway databases

    UniPathwayi UPA00912 .
    BRENDAi 1.1.1.105. 244.
    Reactomei REACT_188277. The canonical retinoid cycle in rods (twilight vision).

    Miscellaneous databases

    NextBioi 297004.
    PROi O55240.
    SOURCEi Search...

    Gene expression databases

    Bgeei O55240.
    Genevestigatori O55240.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    ProtoNeti Search...

    Publicationsi

    1. "The visual cycle retinol dehydrogenase: possible involvement in the 9-cis retinoic acid biosynthetic pathway."
      Driessen C.A., Winkens H.J., Kuhlmann E.D., Janssen A.P., van Vugt A.H., Deutman A.F., Janssen J.J.
      FEBS Lett. 428:135-140(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
      Strain: 129/SvJImported.
      Tissue: Liver1 Publication.
    2. "The identification of a 9-cis retinol dehydrogenase in the mouse embryo reveals a pathway for synthesis of 9-cis retinoic acid."
      Romert A., Tuvendal P., Simon A., Dencker L., Eriksson U.
      Proc. Natl. Acad. Sci. U.S.A. 95:4404-4409(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: LiverImported.
    3. "Biochemical properties, tissue expression, and gene structure of a short chain dehydrogenase/reductase able to catalyze cis-retinol oxidation."
      Gamble M.V., Shang E., Zott R.P., Mertz J.R., Wolgemuth D.J., Blaner W.S.
      J. Lipid Res. 40:2279-2292(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY.
      Strain: 129/SvJ1 Publication, BALB/cImported and C57BL/6JImported.
      Tissue: KidneyImported and LiverImported.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6JImported.
      Tissue: CerebellumImported.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6JImported.
    6. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/NImported.
      Tissue: KidneyImported.
    8. "Gene structure, expression analysis, and membrane topology of RDH4."
      Romert A., Tuvendal P., Tryggvason K., Dencker L., Eriksson U.
      Exp. Cell Res. 256:338-345(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    9. "Disruption of the 11-cis-retinol dehydrogenase gene leads to accumulation of cis-retinols and cis-retinyl esters."
      Driessen C.A., Winkens H.J., Hoffmann K., Kuhlmann L.D., Janssen B.P., Van Vugt A.H., Van Hooser J.P., Wieringa B.E., Deutman A.F., Palczewski K., Ruether K., Janssen J.J.
      Mol. Cell. Biol. 20:4275-4287(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiRDH1_MOUSE
    AccessioniPrimary (citable) accession number: O55240
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2012
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3