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O55240

- RDH1_MOUSE

UniProt

O55240 - RDH1_MOUSE

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Protein
11-cis retinol dehydrogenase
Gene
Rdh5, cRDH, rdh, RDH4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Stereospecific 11-cis retinol dehydrogenase, which catalyzes the final step in the biosynthesis of 11-cis retinaldehyde, the universal chromophore of visual pigments. Also able to oxidize 9-cis-retinol and 13-cis-retinol, but not all-trans-retinol. Active in the presence of NAD as cofactor but not in the presence of NADP.2 Publications

Catalytic activityi

11-cis-retinol-[retinal-binding-protein] + NAD+ = 11-cis-retinal-[retinol-binding-protein] + NADH.2 Publications

Enzyme regulationi

Inhibited by 9-cis-, 13-cis- and all-trans-retinoic acids, with the most potent inhibitor being 13-cis-retinoic acid. Weakly inhibited by oleic acid.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei163 – 1631Substrate By similarity
Active sitei175 – 1751Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi32 – 5625NADP By similarity
Add
BLAST

GO - Molecular functioni

  1. oxidoreductase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. response to stimulus Source: UniProtKB-KW
  2. retinol metabolic process Source: UniProtKB-UniPathway
  3. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.1.1.105. 244.
ReactomeiREACT_188277. The canonical retinoid cycle in rods (twilight vision).
UniPathwayiUPA00912.

Names & Taxonomyi

Protein namesi
Recommended name:
11-cis retinol dehydrogenase (EC:1.1.1.315)
Short name:
11-cis RDH
Short name:
11-cis RoDH
Alternative name(s):
9-cis retinol dehydrogenase
Cis-retinol dehydrogenase
Retinol dehydrogenase 5
Gene namesi
Name:Rdh5
Synonyms:cRDH, rdh, RDH4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1201412. Rdh5.

Subcellular locationi

Membrane; Peripheral membrane protein By similarity. Endoplasmic reticulum lumen By similarity1 Publication

GO - Cellular componenti

  1. cell body Source: Ensembl
  2. endoplasmic reticulum lumen Source: MGI
  3. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice develop normally and are fertile. No abnormalities can be found in the retinal structure, rhodopsin content and fundus appearance of their eyes. Mice display a mild visual phenotype of impaired dark adaptation and accumulation of 11-cis- and 13-cis-retinols and 11-cis- and 13-cis-retinyl esters in the eyes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed prediction
Add
BLAST
Chaini24 – 31829511-cis retinol dehydrogenase
PRO_0000419633Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi160 – 1601N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiO55240.

Expressioni

Tissue specificityi

Expressed in eye, liver, kidney, brain, intestine, placenta, epididymus and submaxillary gland. In eye, strongly expressed in the retinal pigment epithelium, with lower expression levels detected in the inner segment of the photoreceptor cells and in the outer plexiform layer. In kidney, strong expression detected in the distal tubules and the transitional epithelium in the renal pelvis, with weaker expression detected in the epithelium of the outer stripe of the outer zone of the medulla. In liver, detected in hepatocytes in the centrilobular area. In lung, present in Clara cells in the epithelium of the bronchiole, in parenchyma and in cartilage surrounding the secondary bronchi. In skin, expressed in epidermis, hair follicles and mast cells in the dermis. Expressed in heart (1 Publication and 1 Publication). Not detected in heart (1 Publication). Not detected in lung, spleen, skeletal muscle and testis.4 Publications

Developmental stagei

Abundantly expressed during embryonic development, especially in the developing central nervous system and sensory organs, cranial and spinal ganglia and endoderm of foregut and hindgut. At E10, detected along the entire neural tube, the mid- and hindbrain floor, the central canal of the brain vesicles, spinal cord, lung mesenchyme, the trabecular layer of the heart ventricles, endoderm and endodermally-derived structures such as tracheal epithelium and liver. At E11, expressed in the brain vesicles, along the spinal cord, myotome, migrating muscle progenitor cells in the body wall, cells of the genital ridge, spinal ganglion, liver, cerebellar primordium, basal cells of the neuroepithelium of the mesenchephalic flexure, collections of cells in the pons, Rathke's pouch, spinal and cranial ganglia and the floor plate, retina, lens, optic stalks and the neural crest-derived mesenchyme in the anterior eye segment. During eye development, expression restricted to the retinal pigment epithelium of the posterior hemisphere at E18, with expression levels increasing postnatally to P16.2 Publications

Gene expression databases

BgeeiO55240.
GenevestigatoriO55240.

Structurei

3D structure databases

ProteinModelPortaliO55240.
SMRiO55240. Positions 25-284.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00620000087655.
HOVERGENiHBG005482.
InParanoidiO55240.
KOiK00061.
OMAiGPTPWLT.
OrthoDBiEOG7FXZZX.
PhylomeDBiO55240.
TreeFamiTF325617.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55240-1 [UniParc]FASTAAdd to Basket

« Hide

MWLPLLLGAL LWAVLWLLRD RQSLPASDAF IFITGCDSGF GRLLALQLDQ    50
KGFQVLAGCL TPSGAEDLQQ MASSRLHTTL LDITDPQNVQ QVAKWVKTRV 100
GETGLFGLVN NAGVAGIIGP TPWLTQDDFQ RVLSVNTLGP IGVTLALLPL 150
LQQARGRVVN ITSVLGRIAA NGGGYCVSKF GLEAFSDSLR RDMAPFGVQV 200
SIVEPGFFRT PVTNLESLES TLKACWARLP PAIQAHYGEA FLDTYLRVQR 250
RIMNLICDPE LTKVTSCLEH ALTARHPRTR YSPGWDAKLL WLPASYLPAR 300
VVDAVLTWIL PRPAQSVS 318
Length:318
Mass (Da):34,826
Last modified:June 1, 1998 - v1
Checksum:iAA8DB76CD0BF7DFC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X97752 Genomic DNA. Translation: CAA66347.1.
AF013288 mRNA. Translation: AAC25951.1.
AF033195 mRNA. Translation: AAC00491.1.
AF033196 mRNA. Translation: AAC00492.1.
AK135139 mRNA. Translation: BAE22435.1.
AC122380 Genomic DNA. No translation available.
CH466578 Genomic DNA. Translation: EDL24632.1.
BC021372 mRNA. Translation: AAH21372.1.
CCDSiCCDS24298.1.
RefSeqiNP_598767.1. NM_134006.4.
XP_006513450.1. XM_006513387.1.
UniGeneiMm.439856.

Genome annotation databases

EnsembliENSMUST00000026406; ENSMUSP00000026406; ENSMUSG00000025350.
GeneIDi19682.
KEGGimmu:19682.
UCSCiuc007how.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X97752 Genomic DNA. Translation: CAA66347.1 .
AF013288 mRNA. Translation: AAC25951.1 .
AF033195 mRNA. Translation: AAC00491.1 .
AF033196 mRNA. Translation: AAC00492.1 .
AK135139 mRNA. Translation: BAE22435.1 .
AC122380 Genomic DNA. No translation available.
CH466578 Genomic DNA. Translation: EDL24632.1 .
BC021372 mRNA. Translation: AAH21372.1 .
CCDSi CCDS24298.1.
RefSeqi NP_598767.1. NM_134006.4.
XP_006513450.1. XM_006513387.1.
UniGenei Mm.439856.

3D structure databases

ProteinModelPortali O55240.
SMRi O55240. Positions 25-284.
ModBasei Search...

Proteomic databases

PRIDEi O55240.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026406 ; ENSMUSP00000026406 ; ENSMUSG00000025350 .
GeneIDi 19682.
KEGGi mmu:19682.
UCSCi uc007how.1. mouse.

Organism-specific databases

CTDi 5959.
MGIi MGI:1201412. Rdh5.

Phylogenomic databases

GeneTreei ENSGT00620000087655.
HOVERGENi HBG005482.
InParanoidi O55240.
KOi K00061.
OMAi GPTPWLT.
OrthoDBi EOG7FXZZX.
PhylomeDBi O55240.
TreeFami TF325617.

Enzyme and pathway databases

UniPathwayi UPA00912 .
BRENDAi 1.1.1.105. 244.
Reactomei REACT_188277. The canonical retinoid cycle in rods (twilight vision).

Miscellaneous databases

NextBioi 297004.
PROi O55240.
SOURCEi Search...

Gene expression databases

Bgeei O55240.
Genevestigatori O55240.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PR00080. SDRFAMILY.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The visual cycle retinol dehydrogenase: possible involvement in the 9-cis retinoic acid biosynthetic pathway."
    Driessen C.A., Winkens H.J., Kuhlmann E.D., Janssen A.P., van Vugt A.H., Deutman A.F., Janssen J.J.
    FEBS Lett. 428:135-140(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: 129/SvJ.
    Tissue: Liver.
  2. "The identification of a 9-cis retinol dehydrogenase in the mouse embryo reveals a pathway for synthesis of 9-cis retinoic acid."
    Romert A., Tuvendal P., Simon A., Dencker L., Eriksson U.
    Proc. Natl. Acad. Sci. U.S.A. 95:4404-4409(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Liver.
  3. "Biochemical properties, tissue expression, and gene structure of a short chain dehydrogenase/reductase able to catalyze cis-retinol oxidation."
    Gamble M.V., Shang E., Zott R.P., Mertz J.R., Wolgemuth D.J., Blaner W.S.
    J. Lipid Res. 40:2279-2292(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY.
    Strain: 129/SvJ, BALB/c and C57BL/6J.
    Tissue: Kidney and Liver.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  8. "Gene structure, expression analysis, and membrane topology of RDH4."
    Romert A., Tuvendal P., Tryggvason K., Dencker L., Eriksson U.
    Exp. Cell Res. 256:338-345(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  9. "Disruption of the 11-cis-retinol dehydrogenase gene leads to accumulation of cis-retinols and cis-retinyl esters."
    Driessen C.A., Winkens H.J., Hoffmann K., Kuhlmann L.D., Janssen B.P., Van Vugt A.H., Van Hooser J.P., Wieringa B.E., Deutman A.F., Palczewski K., Ruether K., Janssen J.J.
    Mol. Cell. Biol. 20:4275-4287(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiRDH1_MOUSE
AccessioniPrimary (citable) accession number: O55240
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: June 1, 1998
Last modified: September 3, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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