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Protein

Nicotinamide N-methyltransferase

Gene

Nnmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

Catalytic activityi

S-adenosyl-L-methionine + nicotinamide = S-adenosyl-L-homocysteine + 1-methylnicotinamide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20S-adenosyl-L-methionine1
Binding sitei25S-adenosyl-L-methionine1
Binding sitei69S-adenosyl-L-methionine1
Binding sitei90S-adenosyl-L-methionine1
Binding sitei163S-adenosyl-L-methionine1

GO - Molecular functioni

  • nicotinamide N-methyltransferase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-156581 Methylation
R-MMU-197264 Nicotinamide salvaging

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide N-methyltransferase (EC:2.1.1.1)
Gene namesi
Name:Nnmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:1099443 Nnmt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001597071 – 264Nicotinamide N-methyltransferaseAdd BLAST264

Proteomic databases

MaxQBiO55239
PaxDbiO55239
PeptideAtlasiO55239
PRIDEiO55239

PTM databases

iPTMnetiO55239
PhosphoSitePlusiO55239

Expressioni

Gene expression databases

BgeeiENSMUSG00000032271
CleanExiMM_NNMT
ExpressionAtlasiO55239 baseline and differential
GenevisibleiO55239 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034808

Structurei

Secondary structure

1264
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 14Combined sources7
Helixi17 – 25Combined sources9
Beta strandi28 – 30Combined sources3
Helixi32 – 50Combined sources19
Beta strandi51 – 53Combined sources3
Beta strandi56 – 63Combined sources8
Helixi69 – 71Combined sources3
Helixi74 – 76Combined sources3
Beta strandi78 – 86Combined sources9
Helixi88 – 98Combined sources11
Helixi108 – 117Combined sources10
Turni118 – 120Combined sources3
Helixi124 – 134Combined sources11
Beta strandi135 – 140Combined sources6
Beta strandi145 – 147Combined sources3
Turni148 – 151Combined sources4
Beta strandi157 – 164Combined sources8
Helixi166 – 169Combined sources4
Helixi173 – 184Combined sources12
Beta strandi187 – 200Combined sources14
Beta strandi203 – 206Combined sources4
Beta strandi209 – 212Combined sources4
Helixi218 – 227Combined sources10
Beta strandi231 – 238Combined sources8
Turni244 – 246Combined sources3
Beta strandi252 – 258Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I62X-ray1.80A/B/C/D1-264[»]
5XVKX-ray1.88A/B1-264[»]
5YJIX-ray1.99A/B1-264[»]
ProteinModelPortaliO55239
SMRiO55239
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO55239

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni63 – 64S-adenosyl-L-methionine binding2
Regioni85 – 87S-adenosyl-L-methionine binding3
Regioni142 – 143S-adenosyl-L-methionine binding2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFTZ Eukaryota
ENOG41128ZR LUCA
GeneTreeiENSGT00390000011708
HOGENOMiHOG000013229
HOVERGENiHBG000797
InParanoidiO55239
KOiK00541
OMAiEWFEVIS
OrthoDBiEOG091G0EF7
PhylomeDBiO55239
TreeFamiTF313114

Family and domain databases

InterProiView protein in InterPro
IPR025820 NNMT/PNMT/TEMT_CS
IPR000940 NNMT_TEMT_trans
IPR029063 SAM-dependent_MTases
PANTHERiPTHR10867 PTHR10867, 1 hit
PfamiView protein in Pfam
PF01234 NNMT_PNMT_TEMT, 1 hit
PIRSFiPIRSF000384 PNMTase, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS01100 NNMT_PNMT_TEMT, 1 hit
PS51681 SAM_MT_NNMT_PNMT_TEMT, 1 hit

Sequencei

Sequence statusi: Complete.

O55239-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESGFTSKDT YLSHFNPRDY LEKYYSFGSR HCAENEILRH LLKNLFKIFC
60 70 80 90 100
LGAVKGELLI DIGSGPTIYQ LLSACESFTE IIVSDYTDQN LWELQKWLKK
110 120 130 140 150
EPGAFDWSPV VTYVCDLEGN RMKGPEKEEK LRRAIKQVLK CDVTQSQPLG
160 170 180 190 200
GVSLPPADCL LSTLCLDAAC PDLPAYRTAL RNLGSLLKPG GFLVMVDALK
210 220 230 240 250
SSYYMIGEQK FSSLPLGWET VRDAVEEAGY TIEQFEVISQ NYSSTTSNNE
260
GLFSLVGRKP GRSE
Length:264
Mass (Da):29,598
Last modified:June 1, 1998 - v1
Checksum:iDC3FE92738B7EC90
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86105 mRNA Translation: AAB94331.1
U86106 mRNA Translation: AAB94332.1
U86108 mRNA Translation: AAB94334.1
U86107 mRNA Translation: AAB94333.1
AF044960, AF029756 Genomic DNA Translation: AAC77360.1
BC028757 mRNA Translation: AAH28757.1
CCDSiCCDS23157.1
RefSeqiNP_035054.1, NM_010924.3
XP_011240719.1, XM_011242417.1
UniGeneiMm.458542
Mm.8362

Genome annotation databases

EnsembliENSMUST00000034808; ENSMUSP00000034808; ENSMUSG00000032271
GeneIDi18113
KEGGimmu:18113
UCSCiuc009pik.1 mouse

Entry informationi

Entry nameiNNMT_MOUSE
AccessioniPrimary (citable) accession number: O55239
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: May 23, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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