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Protein

Nicotinamide N-methyltransferase

Gene

Nnmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

Catalytic activityi

S-adenosyl-L-methionine + nicotinamide = S-adenosyl-L-homocysteine + 1-methylnicotinamide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20S-adenosyl-L-methionine1
Binding sitei25S-adenosyl-L-methionine1
Binding sitei69S-adenosyl-L-methionine1
Binding sitei90S-adenosyl-L-methionine1
Binding sitei163S-adenosyl-L-methionine1

GO - Molecular functioni

  • nicotinamide N-methyltransferase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-156581. Methylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide N-methyltransferase (EC:2.1.1.1)
Gene namesi
Name:Nnmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1099443. Nnmt.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001597071 – 264Nicotinamide N-methyltransferaseAdd BLAST264

Proteomic databases

MaxQBiO55239.
PaxDbiO55239.
PeptideAtlasiO55239.
PRIDEiO55239.

PTM databases

iPTMnetiO55239.
PhosphoSitePlusiO55239.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032271.
CleanExiMM_NNMT.
ExpressionAtlasiO55239. baseline and differential.
GenevisibleiO55239. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034808.

Structurei

Secondary structure

1264
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 14Combined sources7
Helixi17 – 25Combined sources9
Helixi32 – 50Combined sources19
Beta strandi51 – 53Combined sources3
Beta strandi56 – 63Combined sources8
Helixi69 – 71Combined sources3
Helixi74 – 76Combined sources3
Beta strandi78 – 86Combined sources9
Helixi88 – 98Combined sources11
Helixi108 – 117Combined sources10
Turni118 – 120Combined sources3
Helixi124 – 134Combined sources11
Beta strandi135 – 140Combined sources6
Beta strandi145 – 147Combined sources3
Turni148 – 151Combined sources4
Beta strandi157 – 164Combined sources8
Helixi166 – 169Combined sources4
Helixi173 – 184Combined sources12
Beta strandi187 – 200Combined sources14
Beta strandi203 – 206Combined sources4
Beta strandi209 – 212Combined sources4
Helixi218 – 227Combined sources10
Beta strandi231 – 238Combined sources8
Turni244 – 246Combined sources3
Beta strandi252 – 258Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I62X-ray1.80A/B/C/D1-264[»]
ProteinModelPortaliO55239.
SMRiO55239.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO55239.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni63 – 64S-adenosyl-L-methionine binding2
Regioni85 – 87S-adenosyl-L-methionine binding3
Regioni142 – 143S-adenosyl-L-methionine binding2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFTZ. Eukaryota.
ENOG41128ZR. LUCA.
GeneTreeiENSGT00390000011708.
HOGENOMiHOG000013229.
HOVERGENiHBG000797.
InParanoidiO55239.
KOiK00541.
OMAiEWFEVIS.
OrthoDBiEOG091G0EF7.
PhylomeDBiO55239.
TreeFamiTF313114.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025820. NNMT/PNMT/TEMT_CS.
IPR000940. NNMT_TEMT_trans.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10867. PTHR10867. 1 hit.
PfamiPF01234. NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000384. PNMTase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01100. NNMT_PNMT_TEMT. 1 hit.
PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O55239-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESGFTSKDT YLSHFNPRDY LEKYYSFGSR HCAENEILRH LLKNLFKIFC
60 70 80 90 100
LGAVKGELLI DIGSGPTIYQ LLSACESFTE IIVSDYTDQN LWELQKWLKK
110 120 130 140 150
EPGAFDWSPV VTYVCDLEGN RMKGPEKEEK LRRAIKQVLK CDVTQSQPLG
160 170 180 190 200
GVSLPPADCL LSTLCLDAAC PDLPAYRTAL RNLGSLLKPG GFLVMVDALK
210 220 230 240 250
SSYYMIGEQK FSSLPLGWET VRDAVEEAGY TIEQFEVISQ NYSSTTSNNE
260
GLFSLVGRKP GRSE
Length:264
Mass (Da):29,598
Last modified:June 1, 1998 - v1
Checksum:iDC3FE92738B7EC90
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86105 mRNA. Translation: AAB94331.1.
U86106 mRNA. Translation: AAB94332.1.
U86108 mRNA. Translation: AAB94334.1.
U86107 mRNA. Translation: AAB94333.1.
AF044960, AF029756 Genomic DNA. Translation: AAC77360.1.
BC028757 mRNA. Translation: AAH28757.1.
CCDSiCCDS23157.1.
RefSeqiNP_035054.1. NM_010924.3.
XP_011240719.1. XM_011242417.1.
UniGeneiMm.458542.
Mm.8362.

Genome annotation databases

EnsembliENSMUST00000034808; ENSMUSP00000034808; ENSMUSG00000032271.
GeneIDi18113.
KEGGimmu:18113.
UCSCiuc009pik.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86105 mRNA. Translation: AAB94331.1.
U86106 mRNA. Translation: AAB94332.1.
U86108 mRNA. Translation: AAB94334.1.
U86107 mRNA. Translation: AAB94333.1.
AF044960, AF029756 Genomic DNA. Translation: AAC77360.1.
BC028757 mRNA. Translation: AAH28757.1.
CCDSiCCDS23157.1.
RefSeqiNP_035054.1. NM_010924.3.
XP_011240719.1. XM_011242417.1.
UniGeneiMm.458542.
Mm.8362.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I62X-ray1.80A/B/C/D1-264[»]
ProteinModelPortaliO55239.
SMRiO55239.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034808.

PTM databases

iPTMnetiO55239.
PhosphoSitePlusiO55239.

Proteomic databases

MaxQBiO55239.
PaxDbiO55239.
PeptideAtlasiO55239.
PRIDEiO55239.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034808; ENSMUSP00000034808; ENSMUSG00000032271.
GeneIDi18113.
KEGGimmu:18113.
UCSCiuc009pik.1. mouse.

Organism-specific databases

CTDi4837.
MGIiMGI:1099443. Nnmt.

Phylogenomic databases

eggNOGiENOG410IFTZ. Eukaryota.
ENOG41128ZR. LUCA.
GeneTreeiENSGT00390000011708.
HOGENOMiHOG000013229.
HOVERGENiHBG000797.
InParanoidiO55239.
KOiK00541.
OMAiEWFEVIS.
OrthoDBiEOG091G0EF7.
PhylomeDBiO55239.
TreeFamiTF313114.

Enzyme and pathway databases

ReactomeiR-MMU-156581. Methylation.

Miscellaneous databases

EvolutionaryTraceiO55239.
PROiO55239.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032271.
CleanExiMM_NNMT.
ExpressionAtlasiO55239. baseline and differential.
GenevisibleiO55239. MM.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025820. NNMT/PNMT/TEMT_CS.
IPR000940. NNMT_TEMT_trans.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10867. PTHR10867. 1 hit.
PfamiPF01234. NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000384. PNMTase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01100. NNMT_PNMT_TEMT. 1 hit.
PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNNMT_MOUSE
AccessioniPrimary (citable) accession number: O55239
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.