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Protein

Nicotinamide N-methyltransferase

Gene

Nnmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

Catalytic activityi

S-adenosyl-L-methionine + nicotinamide = S-adenosyl-L-homocysteine + 1-methylnicotinamide.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201S-adenosyl-L-methionine
Binding sitei25 – 251S-adenosyl-L-methionine
Binding sitei69 – 691S-adenosyl-L-methionine
Binding sitei90 – 901S-adenosyl-L-methionine
Binding sitei163 – 1631S-adenosyl-L-methionine

GO - Molecular functioni

  • nicotinamide N-methyltransferase activity Source: MGI

GO - Biological processi

  • methylation Source: MGI
  • organ regeneration Source: Ensembl
  • response to drug Source: Ensembl
  • response to organonitrogen compound Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-156581. Methylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide N-methyltransferase (EC:2.1.1.1)
Gene namesi
Name:Nnmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1099443. Nnmt.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Nicotinamide N-methyltransferasePRO_0000159707Add
BLAST

Proteomic databases

MaxQBiO55239.
PaxDbiO55239.
PeptideAtlasiO55239.
PRIDEiO55239.

PTM databases

iPTMnetiO55239.
PhosphoSiteiO55239.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032271.
CleanExiMM_NNMT.
ExpressionAtlasiO55239. baseline and differential.
GenevisibleiO55239. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034808.

Structurei

Secondary structure

1
264
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 147Combined sources
Helixi17 – 259Combined sources
Helixi32 – 5019Combined sources
Beta strandi51 – 533Combined sources
Beta strandi56 – 638Combined sources
Helixi69 – 713Combined sources
Helixi74 – 763Combined sources
Beta strandi78 – 869Combined sources
Helixi88 – 9811Combined sources
Helixi108 – 11710Combined sources
Turni118 – 1203Combined sources
Helixi124 – 13411Combined sources
Beta strandi135 – 1406Combined sources
Beta strandi145 – 1473Combined sources
Turni148 – 1514Combined sources
Beta strandi157 – 1648Combined sources
Helixi166 – 1694Combined sources
Helixi173 – 18412Combined sources
Beta strandi187 – 20014Combined sources
Beta strandi203 – 2064Combined sources
Beta strandi209 – 2124Combined sources
Helixi218 – 22710Combined sources
Beta strandi231 – 2388Combined sources
Turni244 – 2463Combined sources
Beta strandi252 – 2587Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I62X-ray1.80A/B/C/D1-264[»]
ProteinModelPortaliO55239.
SMRiO55239. Positions 4-264.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO55239.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni63 – 642S-adenosyl-L-methionine binding
Regioni85 – 873S-adenosyl-L-methionine binding
Regioni142 – 1432S-adenosyl-L-methionine binding

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFTZ. Eukaryota.
ENOG41128ZR. LUCA.
GeneTreeiENSGT00390000011708.
HOGENOMiHOG000013229.
HOVERGENiHBG000797.
InParanoidiO55239.
KOiK00541.
OMAiEWFEVIS.
OrthoDBiEOG091G0EF7.
PhylomeDBiO55239.
TreeFamiTF313114.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025820. NNMT/PNMT/TEMT_CS.
IPR000940. NNMT_TEMT_trans.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10867. PTHR10867. 1 hit.
PfamiPF01234. NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000384. PNMTase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01100. NNMT_PNMT_TEMT. 1 hit.
PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O55239-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESGFTSKDT YLSHFNPRDY LEKYYSFGSR HCAENEILRH LLKNLFKIFC
60 70 80 90 100
LGAVKGELLI DIGSGPTIYQ LLSACESFTE IIVSDYTDQN LWELQKWLKK
110 120 130 140 150
EPGAFDWSPV VTYVCDLEGN RMKGPEKEEK LRRAIKQVLK CDVTQSQPLG
160 170 180 190 200
GVSLPPADCL LSTLCLDAAC PDLPAYRTAL RNLGSLLKPG GFLVMVDALK
210 220 230 240 250
SSYYMIGEQK FSSLPLGWET VRDAVEEAGY TIEQFEVISQ NYSSTTSNNE
260
GLFSLVGRKP GRSE
Length:264
Mass (Da):29,598
Last modified:June 1, 1998 - v1
Checksum:iDC3FE92738B7EC90
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86105 mRNA. Translation: AAB94331.1.
U86106 mRNA. Translation: AAB94332.1.
U86108 mRNA. Translation: AAB94334.1.
U86107 mRNA. Translation: AAB94333.1.
AF044960, AF029756 Genomic DNA. Translation: AAC77360.1.
BC028757 mRNA. Translation: AAH28757.1.
CCDSiCCDS23157.1.
RefSeqiNP_035054.1. NM_010924.3.
XP_011240719.1. XM_011242417.1.
UniGeneiMm.458542.
Mm.8362.

Genome annotation databases

EnsembliENSMUST00000034808; ENSMUSP00000034808; ENSMUSG00000032271.
GeneIDi18113.
KEGGimmu:18113.
UCSCiuc009pik.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86105 mRNA. Translation: AAB94331.1.
U86106 mRNA. Translation: AAB94332.1.
U86108 mRNA. Translation: AAB94334.1.
U86107 mRNA. Translation: AAB94333.1.
AF044960, AF029756 Genomic DNA. Translation: AAC77360.1.
BC028757 mRNA. Translation: AAH28757.1.
CCDSiCCDS23157.1.
RefSeqiNP_035054.1. NM_010924.3.
XP_011240719.1. XM_011242417.1.
UniGeneiMm.458542.
Mm.8362.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I62X-ray1.80A/B/C/D1-264[»]
ProteinModelPortaliO55239.
SMRiO55239. Positions 4-264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034808.

PTM databases

iPTMnetiO55239.
PhosphoSiteiO55239.

Proteomic databases

MaxQBiO55239.
PaxDbiO55239.
PeptideAtlasiO55239.
PRIDEiO55239.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034808; ENSMUSP00000034808; ENSMUSG00000032271.
GeneIDi18113.
KEGGimmu:18113.
UCSCiuc009pik.1. mouse.

Organism-specific databases

CTDi4837.
MGIiMGI:1099443. Nnmt.

Phylogenomic databases

eggNOGiENOG410IFTZ. Eukaryota.
ENOG41128ZR. LUCA.
GeneTreeiENSGT00390000011708.
HOGENOMiHOG000013229.
HOVERGENiHBG000797.
InParanoidiO55239.
KOiK00541.
OMAiEWFEVIS.
OrthoDBiEOG091G0EF7.
PhylomeDBiO55239.
TreeFamiTF313114.

Enzyme and pathway databases

ReactomeiR-MMU-156581. Methylation.

Miscellaneous databases

EvolutionaryTraceiO55239.
PROiO55239.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032271.
CleanExiMM_NNMT.
ExpressionAtlasiO55239. baseline and differential.
GenevisibleiO55239. MM.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025820. NNMT/PNMT/TEMT_CS.
IPR000940. NNMT_TEMT_trans.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10867. PTHR10867. 1 hit.
PfamiPF01234. NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000384. PNMTase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01100. NNMT_PNMT_TEMT. 1 hit.
PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNNMT_MOUSE
AccessioniPrimary (citable) accession number: O55239
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.