Reviewed, UniProtKB/Swiss-Prot O55239 (NNMT_MOUSE)

Names and origin

Protein names

Recommended name:
    Nicotinamide N-methyltransferase
    EC=2.1.1.1

Gene names

Name:

Nnmt

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
Catalytic activity	S-adenosyl-L-methionine + nicotinamide = S-adenosyl-L-homocysteine + 1-methylnicotinamide.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the NNMT/PNMT/TEMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nicotinamide N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 264

264

Nicotinamide N-methyltransferase

PRO_0000159707

Regions

Region

63 – 64

2

S-adenosyl-L-methionine binding

Region

85 – 87

3

S-adenosyl-L-methionine binding

Region

142 – 143

2

S-adenosyl-L-methionine binding

Sites

Binding site

20

1

S-adenosyl-L-methionine

Binding site

25

1

S-adenosyl-L-methionine

Binding site

69

1

S-adenosyl-L-methionine

Binding site

90

1

S-adenosyl-L-methionine

Binding site

163

1

S-adenosyl-L-methionine

Secondary structure

1

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264

Helix Strand Turn

Details...

Helix

8 – 14

7

Helix

17 – 25

9

Helix

32 – 50

19

Beta strand

51 – 53

3

Beta strand

56 – 63

8

Helix

69 – 71

3

Helix

74 – 76

3

Beta strand

78 – 86

9

Helix

88 – 98

11

Helix

108 – 117

10

Turn

118 – 120

3

Helix

124 – 134

11

Beta strand

135 – 140

6

Beta strand

145 – 147

3

Turn

148 – 151

4

Beta strand

157 – 164

8

Helix

166 – 169

4

Helix

173 – 184

12

Beta strand

187 – 200

14

Beta strand

203 – 206

4

Beta strand

209 – 212

4

Helix

218 – 227

10

Beta strand

231 – 238

8

Turn

244 – 246

3

Beta strand

252 – 258

7

Sequences

	Sequence		Length	Mass (Da)
	O55239-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: DC3FE92738B7EC90	FASTA	264	29,598
	10 20 30 40 50 60 MESGFTSKDT YLSHFNPRDY LEKYYSFGSR HCAENEILRH LLKNLFKIFC LGAVKGELLI 70 80 90 100 110 120 DIGSGPTIYQ LLSACESFTE IIVSDYTDQN LWELQKWLKK EPGAFDWSPV VTYVCDLEGN 130 140 150 160 170 180 RMKGPEKEEK LRRAIKQVLK CDVTQSQPLG GVSLPPADCL LSTLCLDAAC PDLPAYRTAL 190 200 210 220 230 240 RNLGSLLKPG GFLVMVDALK SSYYMIGEQK FSSLPLGWET VRDAVEEAGY TIEQFEVISQ 250 260 NYSSTTSNNE GLFSLVGRKP GRSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse liver nicotinamide N-methyltransferase: cDNA cloning, expression, and nucleotide sequence polymorphisms." Yan L., Otterness D.M., Craddock T.L., Weinshilboum R.M. Biochem. Pharmacol. 54:1139-1149(1997) [PubMed: 9464457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AKR/J, C3H/HeJ, C57BL/6J, C57BR/cdJ and DBA/2J. Tissue: Liver.
[2]	"Mouse nicotinamide N-methyltransferase gene: molecular cloning, structural characterization, and chromosomal localization." Yan L., Otterness D.M., Kozak C.A., Weinshilboum R.M. DNA Cell Biol. 17:659-667(1998) [PubMed: 9726248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C3H/HeJ.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary gland.
[4]	"The crystal structure of mouse nicotinamide N-methyltransferase in complex with SAH." Structural genomics consortium (SGC) Submitted (SEP-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U86105 mRNA. Translation: AAB94331.1.
U86106 mRNA. Translation: AAB94332.1.
U86108 mRNA. Translation: AAB94334.1.
U86107 mRNA. Translation: AAB94333.1.
AF044960, AF029756 Genomic DNA. Translation: AAC77360.1.
BC028757 mRNA. Translation: AAH28757.1.

IPI

IPI00116723.

RefSeq

NP_035054.1.

UniGene

Mm.458542
Mm.8362

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2I62	X-ray	1.80	A/B/C/D	1-264	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

O55239.

Proteomic databases

PRIDE

O55239.

Genome annotation databases

Ensembl

ENSMUST00000034808; ENSMUSP00000034808; ENSMUSG00000032271; Mus musculus. [Genome view]

GeneID

18113.

KEGG

mmu:18113.

NMPDR

fig|10090.3.peg.20135.

UCSC

uc009pik.1. mouse.

Organism-specific databases

CTD

18113.

MGI

MGI:1099443. Nnmt.

Phylogenomic databases

HOGENOM

O55239.

HOVERGEN

O55239.

OMA

DWSPVVT.

Enzyme and pathway databases

BRENDA

2.1.1.1. 244.

Gene expression databases

ArrayExpress

O55239.

Bgee

O55239.

CleanEx

MM_NNMT.

Genevestigator

O55239.

GermOnline

ENSMUSG00000032271. Mus musculus.

Family and domain databases

InterPro

IPR000940. NNMT_TEMT_trans.
[Graphical view]

PANTHER

PTHR10867. NNMT_TEMT_trans. 1 hit.

Pfam

PF01234. NNMT_PNMT_TEMT. 1 hit.
[Graphical view]

PIRSF

PIRSF000384. PNMTase. 1 hit.

PROSITE

PS01100. NNMT_PNMT_TEMT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

293311.

SOURCE

Search...

Entry information

Entry name

NNMT_MOUSE

Accession

Primary (citable) accession number: O55239

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 1, 1998
Last modified:	November 3, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)