O55233 (CER1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cerberus

Alternative name(s):
Cerberus-like protein
Short name=Cer-l
Cerberus-related protein

Gene names

Name:	Cer1
Synonyms:	Cerl, Cerr1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytokine that may play a role in anterior neural induction and somite formation during embryogenesis in part, through a BMP-inhibitory mechanism. Can regulate Nodal signaling during gastrulation as well as the formation and patterning of the primitive streak. Ref.1 Ref.2 Ref.3 Ref.4
Subunit structure	Forms monomers and predominantly dimers.
Subcellular location	Secreted Ref.1.
Developmental stage	At E6.5., early and mid-streak stage embryos, was expressed in the anterior visceral endoderm. The expression domain extended from the extraembryonic-embryonic junction to approximately two thirds of the way down the epiblast. By the mid-streak to late streak stage, expressed in the anterior visceral endoderm but was also expressed in the definitive endoderm emanating from the anterior portion of the primitive streak. By the neural plate stage at E7.5 expression was present throughout the anterior definitive endoderm layer including both the midline and anterior lateral endoderm. At the early headfold stage expression was reduced in the anterior lateral region and expression was seen primarily in the foregut endoderm. In early E8.5 embryos expression was restricted to the two most recently formed somites. In E9 and E9.5 embryos expression continued in the two newest formed somites and also in the anterior presomitic mesoderm. Ref.1 Ref.2 Ref.3
Post-translational modification	N-glycosylated. Ref.2
Sequence similarities	Belongs to the DAN family. Contains 1 CTCK (C-terminal cystine knot-like) domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anterior/posterior axis specification Inferred from direct assay PubMed 15004567. Source: MGI bone mineralization Inferred from electronic annotation. Source: Compara cell migration involved in gastrulation Inferred from genetic interaction PubMed 15004567. Source: MGI cellular response to BMP stimulus Inferred from sequence or structural similarity PubMed 16798745. Source: BHF-UCL determination of dorsal identity Inferred from electronic annotation. Source: Compara growth plate cartilage chondrocyte proliferation Inferred from expression pattern PubMed 19113921. Source: BHF-UCL negative regulation of activin receptor signaling pathway Inferred from direct assay PubMed 10748465. Source: BHF-UCL negative regulation of cell proliferation Inferred from genetic interaction PubMed 15004567. Source: MGI negative regulation of mesoderm development Inferred from direct assay PubMed 10748465. Source: BHF-UCL nervous system development Inferred from electronic annotation. Source: Compara sequestering of BMP in extracellular matrix Inferred from direct assay PubMed 10748465. Source: BHF-UCL signal transduction involved in regulation of gene expression Inferred from direct assay PubMed 18462699. Source: MGI ureteric bud development Inferred from mutant phenotype PubMed 22114682. Source: UniProtKB
Cellular_component	extracellular space Inferred from direct assay PubMed 10748465. Source: BHF-UCL
Molecular_function	morphogen activity Inferred from electronic annotation. Source: Compara protein homodimerization activity Inferred from direct assay PubMed 10748465. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	By similarity
	Chain	18 – 272	255	Cerberus		PRO_0000006712
Regions
	Domain	162 – 246	85	CTCK
Amino acid modifications
	Glycosylation	168	1	N-linked (GlcNAc...) Potential
	Glycosylation	222	1	N-linked (GlcNAc...) Potential
	Disulfide bond	162 ↔ 209		By similarity
	Disulfide bond	176 ↔ 223		By similarity
	Disulfide bond	186 ↔ 239		By similarity
	Disulfide bond	190 ↔ 241		By similarity
Experimental info
	Sequence conflict	220	1	M → R in AAB71838. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O55233 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: DD0842A2619AF057	FASTA	272	30,431
	10 20 30 40 50 60 MHLLLVQLLV LLPLGKADLC VDGCQSQGSL SFPLLERGRR DLHVANHEEA EDKPDLFVAV 70 80 90 100 110 120 PHLMGTSLAG EGQRQRGKML SRLGRFWKKP ETEFYPPRDV ESDHVSSGMQ AVTQPADGRK 130 140 150 160 170 180 VERSPLQEEA KRFWHRFMFR KGPAFQGVIL PIKSHEVHWE TCRTVPFNQT IAHEDCQKVV 190 200 210 220 230 240 VQNNLCFGKC SSIRFPGEGA DAHSFCSHCS PTKFTTVHLM LNCTSPTPVV KMVMQVEECQ 250 260 270 CMVKTERGEE RLLLAGSQGS FIPGLPASKT NP

References

[1]	"Cerberus-like is a secreted factor with neutralizing activity expressed in the anterior primitive endoderm of the mouse gastrula." Belo J.A., Bouwmeester T., Leyns L., Kertesz N., Gallo M., Follettie M., De Robertis E.M. Mech. Dev. 68:45-57(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE. Strain: 129/SvJ.
[2]	"Murine cerberus homologue mCer-1: a candidate anterior patterning molecule." Biben C., Stanley E., Fabri L., Kotecha S., Rhinn M., Drinkwater C., Lah M., Wang C.-C., Nash A., Hilton D., Ang S.-L., Mohun T., Harvey R.P. Dev. Biol. 194:135-151(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, GLYCOSYLATION.
[3]	"Expression of the mouse cerberus-related gene, Cerr1, suggests a role in anterior neural induction and somitogenesis." Shawlot W., Deng J.M., Behringer R.R. Proc. Natl. Acad. Sci. U.S.A. 95:6198-6203(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
[4]	"Nodal antagonists in the anterior visceral endoderm prevent the formation of multiple primitive streaks." Perea-Gomez A., Vella F.D., Shawlot W., Oulad-Abdelghani M., Chazaud C., Meno C., Pfister V., Chen L., Robertson E., Hamada H., Behringer R.R., Ang S.L. Dev. Cell 3:745-756(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF012244 Genomic DNA. Translation: AAB71838.1. AF035579 mRNA. Translation: AAC02430.1. AF031896 mRNA. Translation: AAC24461.1.
IPI	IPI00116704.
RefSeq	NP_034017.1. NM_009887.2.
UniGene	Mm.6780.
3D structure databases
ProteinModelPortal	O55233.
SMR	O55233. Positions 147-245.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000102858.
PTM databases
PhosphoSite	O55233.
Proteomic databases
PRIDE	O55233.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000048430; ENSMUSP00000048607; ENSMUSG00000038192.
GeneID	12622.
KEGG	mmu:12622.
Organism-specific databases
CTD	9350.
MGI	MGI:1201414. Cer1.
Phylogenomic databases
eggNOG	NOG40714.
GeneTree	ENSGT00530000063926.
HOGENOM	HOG000231309.
HOVERGEN	HBG050902.
InParanoid	O55233.
KO	K01645.
OMA	CFGKCGS.
OrthoDB	EOG437RFQ.
Gene expression databases
ArrayExpress	O55233.
Bgee	O55233.
CleanEx	MM_CER1.
Genevestigator	O55233.
GermOnline	ENSMUSG00000038192. Mus musculus.
Family and domain databases
InterPro	IPR016860. Cerberus. IPR006207. Cys_knot_C. IPR004133. DAN. [Graphical view]
Pfam	PF03045. DAN. 1 hit. [Graphical view]
PIRSF	PIRSF027807. Cerberus. 1 hit.
SMART	SM00041. CT. 1 hit. [Graphical view]
PROSITE	PS01185. CTCK_1. False negative. PS01225. CTCK_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	281790.
SOURCE	Search...

Entry information

Entry name

CER1_MOUSE

Accession

Primary (citable) accession number: O55233
Secondary accession number(s): O35213

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program