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Protein

Cerberus

Gene

Cer1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytokine that may play a role in anterior neural induction and somite formation during embryogenesis in part, through a BMP-inhibitory mechanism. Can regulate Nodal signaling during gastrulation as well as the formation and patterning of the primitive streak.4 Publications

GO - Molecular functioni

  1. BMP binding Source: MGI
  2. morphogen activity Source: MGI
  3. protein homodimerization activity Source: BHF-UCL

GO - Biological processi

  1. anterior/posterior axis specification Source: MGI
  2. anterior/posterior pattern specification Source: MGI
  3. bone mineralization Source: MGI
  4. cell migration involved in gastrulation Source: MGI
  5. cellular response to BMP stimulus Source: BHF-UCL
  6. determination of dorsal identity Source: MGI
  7. gastrulation Source: MGI
  8. growth plate cartilage chondrocyte proliferation Source: BHF-UCL
  9. negative regulation of activin receptor signaling pathway Source: BHF-UCL
  10. negative regulation of BMP signaling pathway Source: MGI
  11. negative regulation of cell proliferation Source: MGI
  12. negative regulation of mesoderm development Source: BHF-UCL
  13. nervous system development Source: MGI
  14. sequestering of BMP in extracellular matrix Source: BHF-UCL
  15. signal transduction involved in regulation of gene expression Source: MGI
  16. ureteric bud development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Enzyme and pathway databases

ReactomeiREACT_304594. Regulation of signaling by NODAL.
REACT_334226. Signaling by BMP.

Names & Taxonomyi

Protein namesi
Recommended name:
Cerberus
Alternative name(s):
Cerberus-like protein
Short name:
Cer-l
Cerberus-related protein
Gene namesi
Name:Cer1
Synonyms:Cerl, Cerr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1201414. Cer1.

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: MGI
  2. extracellular space Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717By similarityAdd
BLAST
Chaini18 – 272255CerberusPRO_0000006712Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi162 ↔ 209PROSITE-ProRule annotation
Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi176 ↔ 223PROSITE-ProRule annotation
Disulfide bondi186 ↔ 239PROSITE-ProRule annotation
Disulfide bondi190 ↔ 241PROSITE-ProRule annotation
Glycosylationi222 – 2221N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiO55233.

PTM databases

PhosphoSiteiO55233.

Expressioni

Developmental stagei

At E6.5., early and mid-streak stage embryos, was expressed in the anterior visceral endoderm. The expression domain extended from the extraembryonic-embryonic junction to approximately two thirds of the way down the epiblast. By the mid-streak to late streak stage, expressed in the anterior visceral endoderm but was also expressed in the definitive endoderm emanating from the anterior portion of the primitive streak. By the neural plate stage at E7.5 expression was present throughout the anterior definitive endoderm layer including both the midline and anterior lateral endoderm. At the early headfold stage expression was reduced in the anterior lateral region and expression was seen primarily in the foregut endoderm. In early E8.5 embryos expression was restricted to the two most recently formed somites. In E9 and E9.5 embryos expression continued in the two newest formed somites and also in the anterior presomitic mesoderm.3 Publications

Gene expression databases

BgeeiO55233.
CleanExiMM_CER1.
ExpressionAtlasiO55233. baseline and differential.
GenevestigatoriO55233.

Interactioni

Subunit structurei

Forms monomers and predominantly dimers.

Protein-protein interaction databases

BioGridi198677. 1 interaction.
STRINGi10090.ENSMUSP00000102858.

Structurei

3D structure databases

ProteinModelPortaliO55233.
SMRiO55233. Positions 162-242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini162 – 24685CTCKPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DAN family.Curated
Contains 1 CTCK (C-terminal cystine knot-like) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG40714.
GeneTreeiENSGT00530000063926.
HOGENOMiHOG000231309.
HOVERGENiHBG050902.
InParanoidiO55233.
KOiK01645.
OMAiCFGKCGS.
OrthoDBiEOG7VX8X6.
PhylomeDBiO55233.
TreeFamiTF106445.

Family and domain databases

InterProiIPR016860. Cerberus.
IPR006207. Cys_knot_C.
IPR004133. DAN.
[Graphical view]
PfamiPF03045. DAN. 1 hit.
[Graphical view]
PIRSFiPIRSF027807. Cerberus. 1 hit.
SMARTiSM00041. CT. 1 hit.
[Graphical view]
PROSITEiPS01225. CTCK_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLLLVQLLV LLPLGKADLC VDGCQSQGSL SFPLLERGRR DLHVANHEEA
60 70 80 90 100
EDKPDLFVAV PHLMGTSLAG EGQRQRGKML SRLGRFWKKP ETEFYPPRDV
110 120 130 140 150
ESDHVSSGMQ AVTQPADGRK VERSPLQEEA KRFWHRFMFR KGPAFQGVIL
160 170 180 190 200
PIKSHEVHWE TCRTVPFNQT IAHEDCQKVV VQNNLCFGKC SSIRFPGEGA
210 220 230 240 250
DAHSFCSHCS PTKFTTVHLM LNCTSPTPVV KMVMQVEECQ CMVKTERGEE
260 270
RLLLAGSQGS FIPGLPASKT NP
Length:272
Mass (Da):30,431
Last modified:May 31, 1998 - v1
Checksum:iDD0842A2619AF057
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti220 – 2201M → R in AAB71838 (PubMed:9431803).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012244 Genomic DNA. Translation: AAB71838.1.
AF035579 mRNA. Translation: AAC02430.1.
AF031896 mRNA. Translation: AAC24461.1.
CCDSiCCDS18295.1.
RefSeqiNP_034017.1. NM_009887.2.
UniGeneiMm.6780.

Genome annotation databases

EnsembliENSMUST00000048430; ENSMUSP00000048607; ENSMUSG00000038192.
GeneIDi12622.
KEGGimmu:12622.
UCSCiuc008tkl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012244 Genomic DNA. Translation: AAB71838.1.
AF035579 mRNA. Translation: AAC02430.1.
AF031896 mRNA. Translation: AAC24461.1.
CCDSiCCDS18295.1.
RefSeqiNP_034017.1. NM_009887.2.
UniGeneiMm.6780.

3D structure databases

ProteinModelPortaliO55233.
SMRiO55233. Positions 162-242.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198677. 1 interaction.
STRINGi10090.ENSMUSP00000102858.

PTM databases

PhosphoSiteiO55233.

Proteomic databases

PRIDEiO55233.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048430; ENSMUSP00000048607; ENSMUSG00000038192.
GeneIDi12622.
KEGGimmu:12622.
UCSCiuc008tkl.2. mouse.

Organism-specific databases

CTDi9350.
MGIiMGI:1201414. Cer1.

Phylogenomic databases

eggNOGiNOG40714.
GeneTreeiENSGT00530000063926.
HOGENOMiHOG000231309.
HOVERGENiHBG050902.
InParanoidiO55233.
KOiK01645.
OMAiCFGKCGS.
OrthoDBiEOG7VX8X6.
PhylomeDBiO55233.
TreeFamiTF106445.

Enzyme and pathway databases

ReactomeiREACT_304594. Regulation of signaling by NODAL.
REACT_334226. Signaling by BMP.

Miscellaneous databases

NextBioi281790.
PROiO55233.
SOURCEiSearch...

Gene expression databases

BgeeiO55233.
CleanExiMM_CER1.
ExpressionAtlasiO55233. baseline and differential.
GenevestigatoriO55233.

Family and domain databases

InterProiIPR016860. Cerberus.
IPR006207. Cys_knot_C.
IPR004133. DAN.
[Graphical view]
PfamiPF03045. DAN. 1 hit.
[Graphical view]
PIRSFiPIRSF027807. Cerberus. 1 hit.
SMARTiSM00041. CT. 1 hit.
[Graphical view]
PROSITEiPS01225. CTCK_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cerberus-like is a secreted factor with neutralizing activity expressed in the anterior primitive endoderm of the mouse gastrula."
    Belo J.A., Bouwmeester T., Leyns L., Kertesz N., Gallo M., Follettie M., De Robertis E.M.
    Mech. Dev. 68:45-57(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    Strain: 129/SvJ.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, GLYCOSYLATION.
  3. "Expression of the mouse cerberus-related gene, Cerr1, suggests a role in anterior neural induction and somitogenesis."
    Shawlot W., Deng J.M., Behringer R.R.
    Proc. Natl. Acad. Sci. U.S.A. 95:6198-6203(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
  4. "Nodal antagonists in the anterior visceral endoderm prevent the formation of multiple primitive streaks."
    Perea-Gomez A., Vella F.D., Shawlot W., Oulad-Abdelghani M., Chazaud C., Meno C., Pfister V., Chen L., Robertson E., Hamada H., Behringer R.R., Ang S.L.
    Dev. Cell 3:745-756(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCER1_MOUSE
AccessioniPrimary (citable) accession number: O55233
Secondary accession number(s): O35213
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 11, 2005
Last sequence update: May 31, 1998
Last modified: March 31, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.