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O55233 (CER1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cerberus
Alternative name(s):
Cerberus-like protein
Short name=Cer-l
Cerberus-related protein
Gene names
Name:Cer1
Synonyms:Cerl, Cerr1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length272 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytokine that may play a role in anterior neural induction and somite formation during embryogenesis in part, through a BMP-inhibitory mechanism. Can regulate Nodal signaling during gastrulation as well as the formation and patterning of the primitive streak. Ref.1 Ref.2 Ref.3 Ref.4

Subunit structure

Forms monomers and predominantly dimers.

Subcellular location

Secreted Ref.1.

Developmental stage

At E6.5., early and mid-streak stage embryos, was expressed in the anterior visceral endoderm. The expression domain extended from the extraembryonic-embryonic junction to approximately two thirds of the way down the epiblast. By the mid-streak to late streak stage, expressed in the anterior visceral endoderm but was also expressed in the definitive endoderm emanating from the anterior portion of the primitive streak. By the neural plate stage at E7.5 expression was present throughout the anterior definitive endoderm layer including both the midline and anterior lateral endoderm. At the early headfold stage expression was reduced in the anterior lateral region and expression was seen primarily in the foregut endoderm. In early E8.5 embryos expression was restricted to the two most recently formed somites. In E9 and E9.5 embryos expression continued in the two newest formed somites and also in the anterior presomitic mesoderm. Ref.1 Ref.2 Ref.3

Post-translational modification

N-glycosylated. Ref.2

Sequence similarities

Belongs to the DAN family.

Contains 1 CTCK (C-terminal cystine knot-like) domain.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionCytokine
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanterior/posterior axis specification

Inferred from direct assay PubMed 15004567. Source: MGI

anterior/posterior pattern specification

Inferred from direct assay Ref.2. Source: MGI

bone mineralization

Inferred from electronic annotation. Source: Ensembl

cell migration involved in gastrulation

Inferred from genetic interaction PubMed 15004567. Source: MGI

cellular response to BMP stimulus

Inferred from sequence or structural similarity PubMed 16798745. Source: BHF-UCL

determination of dorsal identity

Inferred from electronic annotation. Source: Ensembl

gastrulation

Inferred from genetic interaction PubMed 18241853. Source: MGI

growth plate cartilage chondrocyte proliferation

Inferred from expression pattern PubMed 19113921. Source: BHF-UCL

negative regulation of BMP signaling pathway

Inferred from direct assay Ref.1Ref.2. Source: MGI

negative regulation of activin receptor signaling pathway

Inferred from direct assay PubMed 10748465. Source: BHF-UCL

negative regulation of cell proliferation

Inferred from genetic interaction PubMed 15004567. Source: MGI

negative regulation of mesoderm development

Inferred from direct assay PubMed 10748465. Source: BHF-UCL

nervous system development

Inferred from electronic annotation. Source: Ensembl

sequestering of BMP in extracellular matrix

Inferred from direct assay PubMed 10748465. Source: BHF-UCL

signal transduction involved in regulation of gene expression

Inferred from direct assay PubMed 18462699. Source: MGI

ureteric bud development

Inferred from mutant phenotype PubMed 22114682. Source: UniProtKB

   Cellular_componentextracellular region

Inferred from direct assay Ref.1. Source: MGI

extracellular space

Inferred from direct assay PubMed 10748465. Source: BHF-UCL

   Molecular_functionmorphogen activity

Inferred from electronic annotation. Source: Ensembl

protein homodimerization activity

Inferred from direct assay PubMed 10748465. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 By similarity
Chain18 – 272255Cerberus
PRO_0000006712

Regions

Domain162 – 24685CTCK

Amino acid modifications

Glycosylation1681N-linked (GlcNAc...) Potential
Glycosylation2221N-linked (GlcNAc...) Potential
Disulfide bond162 ↔ 209 By similarity
Disulfide bond176 ↔ 223 By similarity
Disulfide bond186 ↔ 239 By similarity
Disulfide bond190 ↔ 241 By similarity

Experimental info

Sequence conflict2201M → R in AAB71838. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O55233 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: DD0842A2619AF057

FASTA27230,431
        10         20         30         40         50         60 
MHLLLVQLLV LLPLGKADLC VDGCQSQGSL SFPLLERGRR DLHVANHEEA EDKPDLFVAV 

        70         80         90        100        110        120 
PHLMGTSLAG EGQRQRGKML SRLGRFWKKP ETEFYPPRDV ESDHVSSGMQ AVTQPADGRK 

       130        140        150        160        170        180 
VERSPLQEEA KRFWHRFMFR KGPAFQGVIL PIKSHEVHWE TCRTVPFNQT IAHEDCQKVV 

       190        200        210        220        230        240 
VQNNLCFGKC SSIRFPGEGA DAHSFCSHCS PTKFTTVHLM LNCTSPTPVV KMVMQVEECQ 

       250        260        270 
CMVKTERGEE RLLLAGSQGS FIPGLPASKT NP 

« Hide

References

[1]"Cerberus-like is a secreted factor with neutralizing activity expressed in the anterior primitive endoderm of the mouse gastrula."
Belo J.A., Bouwmeester T., Leyns L., Kertesz N., Gallo M., Follettie M., De Robertis E.M.
Mech. Dev. 68:45-57(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
Strain: 129/SvJ.
[2]"Murine cerberus homologue mCer-1: a candidate anterior patterning molecule."
Biben C., Stanley E., Fabri L., Kotecha S., Rhinn M., Drinkwater C., Lah M., Wang C.-C., Nash A., Hilton D., Ang S.-L., Mohun T., Harvey R.P.
Dev. Biol. 194:135-151(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, GLYCOSYLATION.
[3]"Expression of the mouse cerberus-related gene, Cerr1, suggests a role in anterior neural induction and somitogenesis."
Shawlot W., Deng J.M., Behringer R.R.
Proc. Natl. Acad. Sci. U.S.A. 95:6198-6203(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
[4]"Nodal antagonists in the anterior visceral endoderm prevent the formation of multiple primitive streaks."
Perea-Gomez A., Vella F.D., Shawlot W., Oulad-Abdelghani M., Chazaud C., Meno C., Pfister V., Chen L., Robertson E., Hamada H., Behringer R.R., Ang S.L.
Dev. Cell 3:745-756(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF012244 Genomic DNA. Translation: AAB71838.1.
AF035579 mRNA. Translation: AAC02430.1.
AF031896 mRNA. Translation: AAC24461.1.
RefSeqNP_034017.1. NM_009887.2.
UniGeneMm.6780.

3D structure databases

ProteinModelPortalO55233.
SMRO55233. Positions 162-242.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198677. 1 interaction.
STRING10090.ENSMUSP00000102858.

PTM databases

PhosphoSiteO55233.

Proteomic databases

PRIDEO55233.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000048430; ENSMUSP00000048607; ENSMUSG00000038192.
GeneID12622.
KEGGmmu:12622.
UCSCuc008tkl.2. mouse.

Organism-specific databases

CTD9350.
MGIMGI:1201414. Cer1.

Phylogenomic databases

eggNOGNOG40714.
GeneTreeENSGT00530000063926.
HOGENOMHOG000231309.
HOVERGENHBG050902.
InParanoidO55233.
KOK01645.
OMACFGKCGS.
OrthoDBEOG7VX8X6.
PhylomeDBO55233.
TreeFamTF106445.

Gene expression databases

BgeeO55233.
CleanExMM_CER1.
GenevestigatorO55233.

Family and domain databases

InterProIPR016860. Cerberus.
IPR006207. Cys_knot_C.
IPR004133. DAN.
[Graphical view]
PfamPF03045. DAN. 1 hit.
[Graphical view]
PIRSFPIRSF027807. Cerberus. 1 hit.
SMARTSM00041. CT. 1 hit.
[Graphical view]
PROSITEPS01225. CTCK_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio281790.
PROO55233.
SOURCESearch...

Entry information

Entry nameCER1_MOUSE
AccessionPrimary (citable) accession number: O55233
Secondary accession number(s): O35213
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot