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O55232

- OREX_RAT

UniProt

O55232 - OREX_RAT

Protein

Orexin

Gene

Hcrt

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. A modulation effect on luteinizing hormone-releasing hormone (LHRH) secretion also suggests a more minor contribution to the regulation of reproductive function. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.

    GO - Molecular functioni

    1. neuropeptide hormone activity Source: RGD
    2. type 1 hypocretin receptor binding Source: RGD
    3. type 2 hypocretin receptor binding Source: RGD

    GO - Biological processi

    1. behavioral fear response Source: RGD
    2. eating behavior Source: RGD
    3. feeding behavior Source: RGD
    4. negative regulation of DNA replication Source: RGD
    5. negative regulation of potassium ion transport Source: RGD
    6. negative regulation of transmission of nerve impulse Source: RGD
    7. neuropeptide signaling pathway Source: UniProtKB-KW
    8. phospholipase C-activating G-protein coupled receptor signaling pathway Source: RGD
    9. positive regulation of calcium ion transport Source: RGD
    10. positive regulation of cytosolic calcium ion concentration Source: RGD
    11. positive regulation of transmission of nerve impulse Source: RGD
    12. protein kinase C-activating G-protein coupled receptor signaling pathway Source: RGD
    13. regulation of excitatory postsynaptic membrane potential Source: RGD
    14. regulation of neurotransmitter secretion Source: RGD

    Keywords - Molecular functioni

    Neuropeptide

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Orexin
    Alternative name(s):
    Hypocretin
    Short name:
    Hcrt
    Cleaved into the following 2 chains:
    Alternative name(s):
    Hypocretin-1
    Short name:
    Hcrt1
    Alternative name(s):
    Hypocretin-2
    Short name:
    Hcrt2
    Gene namesi
    Name:Hcrt
    Synonyms:Ox, Ppox
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi2786. Hcrt.

    Subcellular locationi

    Rough endoplasmic reticulum
    Note: Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses.

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. perinuclear region of cytoplasm Source: Ensembl
    3. rough endoplasmic reticulum Source: UniProtKB-SubCell
    4. secretory granule Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 32321 PublicationAdd
    BLAST
    Peptidei33 – 6533Orexin-APRO_0000020270Add
    BLAST
    Peptidei69 – 9628Orexin-BPRO_0000020271Add
    BLAST
    Propeptidei97 – 13034PRO_0000020272Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei33 – 331Pyrrolidone carboxylic acid1 Publication
    Disulfide bondi38 ↔ 44
    Disulfide bondi39 ↔ 46
    Modified residuei65 – 651Leucine amide
    Modified residuei96 – 961Methionine amide

    Post-translational modificationi

    Specific enzymatic cleavages at paired basic residues yield the different active peptides.

    Keywords - PTMi

    Amidation, Cleavage on pair of basic residues, Disulfide bond, Pyrrolidone carboxylic acid

    Proteomic databases

    PRIDEiO55232.

    Expressioni

    Tissue specificityi

    Produced by a small group of neurons restricted to the lateral and posterior hypothalamus and perifornical areas. Positive neurons project widely throughout the entire neuroaxis. Particularly abundant projections in the cerebral cortex, olfactory bulb, hippocampus, amygdala, septum, diagonal band of Broca, bed nucleus of the stria terminalis, thalamus, anterior and posterior hypothalamus, midbrain, brainstem, and spinal cord. Immunoreactivity reported in the enteric nervous system and pancreas. In small amount, also detected in the testis.

    Developmental stagei

    Detected as early as embryonic day 18, but expression increased dramatically after the third postnatal week.

    Inductioni

    By nutritional state, up-regulated by fasting, fluid deprivation and insulin-induced hypoglycemia. Orexin-A immunoreactivity varies diurnally and peaks during the dark phase, in the pons and the location of locus coeruleus.

    Gene expression databases

    GenevestigatoriO55232.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000025547.

    Structurei

    3D structure databases

    ProteinModelPortaliO55232.
    SMRiO55232. Positions 33-65, 71-96.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the orexin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG70925.
    GeneTreeiENSGT00390000014272.
    HOGENOMiHOG000230990.
    HOVERGENiHBG000256.
    InParanoidiO55232.
    KOiK05246.
    OMAiSECCRQP.
    OrthoDBiEOG7XSTHD.
    PhylomeDBiO55232.
    TreeFamiTF330756.

    Family and domain databases

    InterProiIPR001704. Orexin.
    [Graphical view]
    PANTHERiPTHR15173. PTHR15173. 1 hit.
    PfamiPF02072. Orexin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037824. Orexin. 1 hit.
    PRINTSiPR01091. OREXINPP.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O55232-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLPSTKVPW AAVTLLLLLL LPPALLSLGV DAQPLPDCCR QKTCSCRLYE    50
    LLHGAGNHAA GILTLGKRRP GPPGLQGRLQ RLLQANGNHA AGILTMGRRA 100
    GAELEPYPCP GRRCPTATAT ALAPRGGSRV 130
    Length:130
    Mass (Da):13,645
    Last modified:June 1, 1998 - v1
    Checksum:i00CAB259EDF2A404
    GO

    Mass spectrometryi

    Molecular mass is 3558.7±0.1 Da from positions 33 - 65. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF041241 mRNA. Translation: AAC40039.1.
    AF019565 mRNA. Translation: AAC02933.1.
    RefSeqiNP_037311.1. NM_013179.2.
    UniGeneiRn.7628.

    Genome annotation databases

    EnsembliENSRNOT00000025547; ENSRNOP00000025547; ENSRNOG00000018892.
    GeneIDi25723.
    KEGGirno:25723.
    UCSCiRGD:2786. rat.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Qui dort dine - Issue 15 of October 2001

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF041241 mRNA. Translation: AAC40039.1 .
    AF019565 mRNA. Translation: AAC02933.1 .
    RefSeqi NP_037311.1. NM_013179.2.
    UniGenei Rn.7628.

    3D structure databases

    ProteinModelPortali O55232.
    SMRi O55232. Positions 33-65, 71-96.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000025547.

    Proteomic databases

    PRIDEi O55232.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000025547 ; ENSRNOP00000025547 ; ENSRNOG00000018892 .
    GeneIDi 25723.
    KEGGi rno:25723.
    UCSCi RGD:2786. rat.

    Organism-specific databases

    CTDi 3060.
    RGDi 2786. Hcrt.

    Phylogenomic databases

    eggNOGi NOG70925.
    GeneTreei ENSGT00390000014272.
    HOGENOMi HOG000230990.
    HOVERGENi HBG000256.
    InParanoidi O55232.
    KOi K05246.
    OMAi SECCRQP.
    OrthoDBi EOG7XSTHD.
    PhylomeDBi O55232.
    TreeFami TF330756.

    Miscellaneous databases

    NextBioi 607825.
    PROi O55232.

    Gene expression databases

    Genevestigatori O55232.

    Family and domain databases

    InterProi IPR001704. Orexin.
    [Graphical view ]
    PANTHERi PTHR15173. PTHR15173. 1 hit.
    Pfami PF02072. Orexin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037824. Orexin. 1 hit.
    PRINTSi PR01091. OREXINPP.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 33-65 AND 69-96, PYROGLUTAMATE FORMATION AT GLN-33, MASS SPECTROMETRY.
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
    3. "Hypocretin/orexin, sleep and narcolepsy."
      Hungs M., Mignot E.
      Bioessays 23:397-408(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    4. "To eat or to sleep? Orexin in the regulation of feeding and wakefulness."
      Willie J.T., Chemelli R.M., Sinton C.M., Yanagisawa M.
      Annu. Rev. Neurosci. 24:429-458(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiOREX_RAT
    AccessioniPrimary (citable) accession number: O55232
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3