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O55232

- OREX_RAT

UniProt

O55232 - OREX_RAT

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Protein
Orexin
Gene
Hcrt, Ox, Ppox
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. A modulation effect on luteinizing hormone-releasing hormone (LHRH) secretion also suggests a more minor contribution to the regulation of reproductive function. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.

GO - Molecular functioni

  1. neuropeptide hormone activity Source: RGD
  2. type 1 hypocretin receptor binding Source: RGD
  3. type 2 hypocretin receptor binding Source: RGD

GO - Biological processi

  1. behavioral fear response Source: RGD
  2. eating behavior Source: RGD
  3. feeding behavior Source: RGD
  4. negative regulation of DNA replication Source: RGD
  5. negative regulation of potassium ion transport Source: RGD
  6. negative regulation of transmission of nerve impulse Source: RGD
  7. neuropeptide signaling pathway Source: UniProtKB-KW
  8. phospholipase C-activating G-protein coupled receptor signaling pathway Source: RGD
  9. positive regulation of calcium ion transport Source: RGD
  10. positive regulation of cytosolic calcium ion concentration Source: RGD
  11. positive regulation of transmission of nerve impulse Source: RGD
  12. protein kinase C-activating G-protein coupled receptor signaling pathway Source: RGD
  13. regulation of excitatory postsynaptic membrane potential Source: RGD
  14. regulation of neurotransmitter secretion Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Neuropeptide

Names & Taxonomyi

Protein namesi
Recommended name:
Orexin
Alternative name(s):
Hypocretin
Short name:
Hcrt
Cleaved into the following 2 chains:
Alternative name(s):
Hypocretin-1
Short name:
Hcrt1
Alternative name(s):
Hypocretin-2
Short name:
Hcrt2
Gene namesi
Name:Hcrt
Synonyms:Ox, Ppox
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi2786. Hcrt.

Subcellular locationi

Rough endoplasmic reticulum
Note: Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses.

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. perinuclear region of cytoplasm Source: Ensembl
  3. rough endoplasmic reticulum Source: UniProtKB-SubCell
  4. secretory granule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 32321 Publication
Add
BLAST
Peptidei33 – 6533Orexin-A1 Publication
PRO_0000020270Add
BLAST
Peptidei69 – 9628Orexin-B
PRO_0000020271Add
BLAST
Propeptidei97 – 13034
PRO_0000020272Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331Pyrrolidone carboxylic acid
Disulfide bondi38 ↔ 44
Disulfide bondi39 ↔ 46
Modified residuei65 – 651Leucine amide
Modified residuei96 – 961Methionine amide

Post-translational modificationi

Specific enzymatic cleavages at paired basic residues yield the different active peptides.

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PRIDEiO55232.

Expressioni

Tissue specificityi

Produced by a small group of neurons restricted to the lateral and posterior hypothalamus and perifornical areas. Positive neurons project widely throughout the entire neuroaxis. Particularly abundant projections in the cerebral cortex, olfactory bulb, hippocampus, amygdala, septum, diagonal band of Broca, bed nucleus of the stria terminalis, thalamus, anterior and posterior hypothalamus, midbrain, brainstem, and spinal cord. Immunoreactivity reported in the enteric nervous system and pancreas. In small amount, also detected in the testis.

Developmental stagei

Detected as early as embryonic day 18, but expression increased dramatically after the third postnatal week.

Inductioni

By nutritional state, up-regulated by fasting, fluid deprivation and insulin-induced hypoglycemia. Orexin-A immunoreactivity varies diurnally and peaks during the dark phase, in the pons and the location of locus coeruleus.

Gene expression databases

GenevestigatoriO55232.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025547.

Structurei

3D structure databases

ProteinModelPortaliO55232.
SMRiO55232. Positions 33-65, 71-96.

Family & Domainsi

Sequence similaritiesi

Belongs to the orexin family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG70925.
GeneTreeiENSGT00390000014272.
HOGENOMiHOG000230990.
HOVERGENiHBG000256.
InParanoidiO55232.
KOiK05246.
OMAiSECCRQP.
OrthoDBiEOG7XSTHD.
PhylomeDBiO55232.
TreeFamiTF330756.

Family and domain databases

InterProiIPR001704. Orexin.
[Graphical view]
PANTHERiPTHR15173. PTHR15173. 1 hit.
PfamiPF02072. Orexin. 1 hit.
[Graphical view]
PIRSFiPIRSF037824. Orexin. 1 hit.
PRINTSiPR01091. OREXINPP.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55232-1 [UniParc]FASTAAdd to Basket

« Hide

MNLPSTKVPW AAVTLLLLLL LPPALLSLGV DAQPLPDCCR QKTCSCRLYE    50
LLHGAGNHAA GILTLGKRRP GPPGLQGRLQ RLLQANGNHA AGILTMGRRA 100
GAELEPYPCP GRRCPTATAT ALAPRGGSRV 130
Length:130
Mass (Da):13,645
Last modified:June 1, 1998 - v1
Checksum:i00CAB259EDF2A404
GO

Mass spectrometryi

Molecular mass is 3558.7±0.1 Da from positions 33 - 65. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF041241 mRNA. Translation: AAC40039.1.
AF019565 mRNA. Translation: AAC02933.1.
RefSeqiNP_037311.1. NM_013179.2.
UniGeneiRn.7628.

Genome annotation databases

EnsembliENSRNOT00000025547; ENSRNOP00000025547; ENSRNOG00000018892.
GeneIDi25723.
KEGGirno:25723.
UCSCiRGD:2786. rat.

Cross-referencesi

Web resourcesi

Protein Spotlight

Qui dort dine - Issue 15 of October 2001

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF041241 mRNA. Translation: AAC40039.1 .
AF019565 mRNA. Translation: AAC02933.1 .
RefSeqi NP_037311.1. NM_013179.2.
UniGenei Rn.7628.

3D structure databases

ProteinModelPortali O55232.
SMRi O55232. Positions 33-65, 71-96.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000025547.

Proteomic databases

PRIDEi O55232.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000025547 ; ENSRNOP00000025547 ; ENSRNOG00000018892 .
GeneIDi 25723.
KEGGi rno:25723.
UCSCi RGD:2786. rat.

Organism-specific databases

CTDi 3060.
RGDi 2786. Hcrt.

Phylogenomic databases

eggNOGi NOG70925.
GeneTreei ENSGT00390000014272.
HOGENOMi HOG000230990.
HOVERGENi HBG000256.
InParanoidi O55232.
KOi K05246.
OMAi SECCRQP.
OrthoDBi EOG7XSTHD.
PhylomeDBi O55232.
TreeFami TF330756.

Miscellaneous databases

NextBioi 607825.
PROi O55232.

Gene expression databases

Genevestigatori O55232.

Family and domain databases

InterProi IPR001704. Orexin.
[Graphical view ]
PANTHERi PTHR15173. PTHR15173. 1 hit.
Pfami PF02072. Orexin. 1 hit.
[Graphical view ]
PIRSFi PIRSF037824. Orexin. 1 hit.
PRINTSi PR01091. OREXINPP.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 33-65 AND 69-96, PYROGLUTAMATE FORMATION AT GLN-33, MASS SPECTROMETRY.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  3. "Hypocretin/orexin, sleep and narcolepsy."
    Hungs M., Mignot E.
    Bioessays 23:397-408(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  4. "To eat or to sleep? Orexin in the regulation of feeding and wakefulness."
    Willie J.T., Chemelli R.M., Sinton C.M., Yanagisawa M.
    Annu. Rev. Neurosci. 24:429-458(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiOREX_RAT
AccessioniPrimary (citable) accession number: O55232
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: June 11, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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