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O55230

- RA51D_MOUSE

UniProt

O55230 - RA51D_MOUSE

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Protein

DNA repair protein RAD51 homolog 4

Gene

Rad51d

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi107 – 1148ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA-dependent ATPase activity Source: UniProtKB
  3. four-way junction DNA binding Source: Ensembl
  4. gamma-tubulin binding Source: UniProtKB
  5. single-stranded DNA binding Source: UniProtKB

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. double-strand break repair via homologous recombination Source: UniProtKB
  3. strand invasion Source: UniProtKB
  4. telomere maintenance Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD51 homolog 4
Alternative name(s):
R51H3
RAD51 homolog D
RAD51-like protein 3
Gene namesi
Name:Rad51d
Synonyms:R51h3, Rad51l3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1261809. Rad51d.

Subcellular locationi

Nucleus By similarity. Chromosometelomere 1 Publication

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. chromosome, telomeric region Source: UniProtKB
  3. Rad51B-Rad51C-Rad51D-XRCC2 complex Source: UniProtKB
  4. replication fork Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

Pathology & Biotechi

Disruption phenotypei

Midgestation lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329DNA repair protein RAD51 homolog 4PRO_0000122943Add
BLAST

Proteomic databases

MaxQBiO55230.
PRIDEiO55230.

PTM databases

PhosphoSiteiO55230.

Expressioni

Tissue specificityi

Highly expressed in brain followed by testis. Also expressed in heart, liver, kidney, spleen, lung and skeletal muscle.

Gene expression databases

BgeeiO55230.
CleanExiMM_RAD51L3.
ExpressionAtlasiO55230. baseline and differential.
GenevestigatoriO55230.

Interactioni

Subunit structurei

Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2; the complex has a ring-like structure arranged into a flat disc around a central channel. In the absence of DNA, the BCDX2 subcomplex XRCC2:RAD51D formed a multimeric ring structure; in the presence of single-stranded DNA it formed a filamentous structure with the ssDNA. Interacts with SWSAP1 and ZSWIM7; involved in homologous recombination repair. Interacts with BLM; required for stimulation of BLM activity by the BCDX2 subcomplex XRCC2:RAD51D (By similarity).By similarity

Protein-protein interaction databases

IntActiO55230. 1 interaction.
MINTiMINT-415416.

Structurei

3D structure databases

ProteinModelPortaliO55230.
SMRiO55230. Positions 1-319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8383Preferencially binds ssDNABy similarityAdd
BLAST
Regioni4 – 7774Interaction with XRCC2Add
BLAST
Regioni77 – 328252Interaction with RAD51CAdd
BLAST

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0468.
HOGENOMiHOG000049134.
HOVERGENiHBG057455.
InParanoidiO55230.
KOiK10871.
OMAiCSLSYKA.
PhylomeDBiO55230.
TreeFamiTF101219.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: At least 2 isoforms are produced.

Isoform 1 (identifier: O55230-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGMLRAGLCP GLTEETVQLL RGRKIKTVAD LAAADLEEVA QKCGLSYKAL
60 70 80 90 100
VALRRVLLAQ FSAFPLNGAD LYEELKTSTA ILSTGIGSLD KLLDAGLYTG
110 120 130 140 150
EVTEIVGGPG SGKTQVCLCV AANVAHSLQQ NVLYVDSNGG MTASRLLQLL
160 170 180 190 200
QARTQDEEKQ ASALQRIQVV RSFDIFRMLD MLQDLRGTIA QQEATSSGAV
210 220 230 240 250
KVVIVDSVTA VVAPLLGGQQ REGLALMMQL ARELKILARD LGVAVVVTNH
260 270 280 290 300
LTRDWDGRRF KPALGRSWSF VPSTRILLDV TEGAGTLGSS QRTVCLTKSP
310 320
RQPTGLQEMI DIGTLGTEEQ SPELPGKQT
Length:329
Mass (Da):35,260
Last modified:June 1, 1998 - v1
Checksum:i6E463822408EF484
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007040 mRNA. Translation: BAA24010.1.
AF034955 mRNA. Translation: AAC40093.1.
Y15570 mRNA. Translation: CAA75679.1.
AK017835 mRNA. Translation: BAB30965.1.
BC049136 mRNA. Translation: AAH49136.1.
CCDSiCCDS36250.1. [O55230-1]
RefSeqiNP_035365.1. NM_011235.4. [O55230-1]
UniGeneiMm.441571.
Mm.9286.

Genome annotation databases

EnsembliENSMUST00000018985; ENSMUSP00000018985; ENSMUSG00000018841. [O55230-1]
GeneIDi19364.
KEGGimmu:19364.
UCSCiuc007knm.1. mouse. [O55230-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007040 mRNA. Translation: BAA24010.1 .
AF034955 mRNA. Translation: AAC40093.1 .
Y15570 mRNA. Translation: CAA75679.1 .
AK017835 mRNA. Translation: BAB30965.1 .
BC049136 mRNA. Translation: AAH49136.1 .
CCDSi CCDS36250.1. [O55230-1 ]
RefSeqi NP_035365.1. NM_011235.4. [O55230-1 ]
UniGenei Mm.441571.
Mm.9286.

3D structure databases

ProteinModelPortali O55230.
SMRi O55230. Positions 1-319.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O55230. 1 interaction.
MINTi MINT-415416.

PTM databases

PhosphoSitei O55230.

Proteomic databases

MaxQBi O55230.
PRIDEi O55230.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000018985 ; ENSMUSP00000018985 ; ENSMUSG00000018841 . [O55230-1 ]
GeneIDi 19364.
KEGGi mmu:19364.
UCSCi uc007knm.1. mouse. [O55230-1 ]

Organism-specific databases

CTDi 5892.
MGIi MGI:1261809. Rad51d.

Phylogenomic databases

eggNOGi COG0468.
HOGENOMi HOG000049134.
HOVERGENi HBG057455.
InParanoidi O55230.
KOi K10871.
OMAi CSLSYKA.
PhylomeDBi O55230.
TreeFami TF101219.

Miscellaneous databases

NextBioi 296433.
PROi O55230.
SOURCEi Search...

Gene expression databases

Bgeei O55230.
CleanExi MM_RAD51L3.
ExpressionAtlasi O55230. baseline and differential.
Genevestigatori O55230.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
[Graphical view ]
Pfami PF08423. Rad51. 1 hit.
[Graphical view ]
PIRSFi PIRSF005856. Rad51. 1 hit.
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS50162. RECA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification, characterization, and genetic mapping of Rad51d, a new mouse and human RAD51/RecA-related gene."
    Pittman D.L., Weinberg L.R., Schimenti J.C.
    Genomics 49:103-111(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
  2. "Isolation of novel human and mouse genes of the recA/RAD51 recombination-repair gene family."
    Cartwright R., Dunn A.M., Simpson P.J., Tambini C.E., Thacker J.
    Nucleic Acids Res. 26:1653-1659(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Kawabata M.
    Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  6. "Midgestation lethality in mice deficient for the RecA-related gene, Rad51d/Rad51l3."
    Pittman D.L., Schimenti J.C.
    Genesis 26:167-173(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Telomere maintenance requires the RAD51D recombination/repair protein."
    Tarsounas M., Munoz P., Claas A., Smiraldo P.G., Pittman D.L., Blasco M.A., West S.C.
    Cell 117:337-347(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "Domain mapping of the Rad51 paralog protein complexes."
    Miller K.A., Sawicka D., Barsky D., Albala J.S.
    Nucleic Acids Res. 32:169-178(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAD51C AND XRCC2.

Entry informationi

Entry nameiRA51D_MOUSE
AccessioniPrimary (citable) accession number: O55230
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3