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O55229

- CHKB_MOUSE

UniProt

O55229 - CHKB_MOUSE

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Protein

Choline/ethanolamine kinase

Gene

Chkb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis (By similarity).By similarity

Catalytic activityi

ATP + choline = ADP + phosphocholine.
ATP + ethanolamine = ADP + O-phosphoethanolamine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041ATPBy similarity
Binding sitei244 – 2441ATPBy similarity
Binding sitei264 – 2641ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 817ATPBy similarity
Nucleotide bindingi146 – 1527ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. choline kinase activity Source: UniProtKB
  3. ethanolamine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. CDP-choline pathway Source: GOC
  2. muscle organ development Source: MGI
  3. phosphatidylcholine biosynthetic process Source: MGI
  4. phosphatidylethanolamine biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_199009. Synthesis of PE.
REACT_207335. Synthesis of PC.
UniPathwayiUPA00558; UER00741.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline/ethanolamine kinase
Alternative name(s):
Choline kinase beta (EC:2.7.1.32)
Short name:
CK
Short name:
CKB
Ethanolamine kinase (EC:2.7.1.82)
Short name:
EK
choline/ethanolamine kinase beta
Short name:
CKEKB
Gene namesi
Name:Chkb
Synonyms:Chetk, Chkl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1328313. Chkb.

Pathology & Biotechi

Involvement in diseasei

Defects in Chkb are a cause of rostrocaudal muscular dystrophy (rmd). The disease is characterized by rapidly progressive muscular dystrophy and neonatal forelimb bone deformity. The dystrophy is only evident in skeletal muscle tissues in an unusual rostral-to-caudal gradient.1 Publication

Disruption phenotypei

Hindlimb muscular dystrophy. Hindlimb skeletal muscle tissue exhibits impaired phosphatidylcholine biosynthesis and increased phosphatidylcholine catabolism, with concomitant accumulation of choline. Mitochondria are abnormally large and exhibit decreased inner membrane potential.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 394393Choline/ethanolamine kinasePRO_0000206223Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO55229.
PaxDbiO55229.
PRIDEiO55229.

PTM databases

PhosphoSiteiO55229.

Expressioni

Tissue specificityi

Expressed ubiquitously with the highest level in testis.

Gene expression databases

BgeeiO55229.
CleanExiMM_CHKB.
ExpressionAtlasiO55229. baseline and differential.
GenevestigatoriO55229.

Interactioni

Subunit structurei

Homodimer, and heterodimer with CHKA.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO55229.
SMRiO55229. Positions 42-390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni77 – 793Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the choline/ethanolamine kinase family.Curated

Phylogenomic databases

eggNOGiCOG0510.
HOGENOMiHOG000041274.
HOVERGENiHBG050943.
InParanoidiO55229.
KOiK14156.
OMAiFTKEPRW.
OrthoDBiEOG72VH68.
PhylomeDBiO55229.
TreeFamiTF313549.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55229-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAADGTGVVG GGAVGGGLPK DGLQDAKCPE PIPNRRRASS LSRDAQRRAY
60 70 80 90 100
QWCREYLGGA WRRARPEELS VCPVSGGLSN LLFRCSLPNH VPSVGGEPRE
110 120 130 140 150
VLLRLYGAIL QGVDSLVLES VMFAILAERS LGPQLYGVFP EGRLEQYLPS
160 170 180 190 200
RPLKTQELRD PVLSGAIATR MARFHGMEMP FTKEPRWLFG TMERYLKQIQ
210 220 230 240 250
DLPSTSLPQM NLVEMYSLKD EMNSLRKLLD DTPSPVVFCH NDIQEGNILL
260 270 280 290 300
LSEPDSDDNL MLVDFEYSSY NYRGFDIGNH FCEWVYDYTY EEWPFYKARP
310 320 330 340 350
TDYPTREQQL HFIRHYLAEV QKGEILSEEE QKKREEELLL EISRYSLASH
360 370 380 390
FFWGLWSTLQ ASMSTIEFGY LEYAQSRFQF YFQQKGQLTS SPSS
Length:394
Mass (Da):45,126
Last modified:January 23, 2007 - v3
Checksum:iA5EE79EC551D07A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011001 Transcribed RNA. Translation: BAA24897.1.
AB011000 mRNA. Translation: BAA24896.1.
CCDSiCCDS27750.1.
RefSeqiNP_031718.1. NM_007692.6.
UniGeneiMm.485045.

Genome annotation databases

EnsembliENSMUST00000023289; ENSMUSP00000023289; ENSMUSG00000022617.
GeneIDi12651.
KEGGimmu:12651.
UCSCiuc007xgs.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011001 Transcribed RNA. Translation: BAA24897.1 .
AB011000 mRNA. Translation: BAA24896.1 .
CCDSi CCDS27750.1.
RefSeqi NP_031718.1. NM_007692.6.
UniGenei Mm.485045.

3D structure databases

ProteinModelPortali O55229.
SMRi O55229. Positions 42-390.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei O55229.

Proteomic databases

MaxQBi O55229.
PaxDbi O55229.
PRIDEi O55229.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023289 ; ENSMUSP00000023289 ; ENSMUSG00000022617 .
GeneIDi 12651.
KEGGi mmu:12651.
UCSCi uc007xgs.2. mouse.

Organism-specific databases

CTDi 1120.
MGIi MGI:1328313. Chkb.

Phylogenomic databases

eggNOGi COG0510.
HOGENOMi HOG000041274.
HOVERGENi HBG050943.
InParanoidi O55229.
KOi K14156.
OMAi FTKEPRW.
OrthoDBi EOG72VH68.
PhylomeDBi O55229.
TreeFami TF313549.

Enzyme and pathway databases

UniPathwayi UPA00558 ; UER00741 .
Reactomei REACT_199009. Synthesis of PE.
REACT_207335. Synthesis of PC.

Miscellaneous databases

NextBioi 281860.
PROi O55229.
SOURCEi Search...

Gene expression databases

Bgeei O55229.
CleanExi MM_CHKB.
ExpressionAtlasi O55229. baseline and differential.
Genevestigatori O55229.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of mouse choline kinase and choline/ethanolamine kinase: their sequence comparison to the respective rat homologs."
    Aoyama C., Nakashima K., Ishidate K.
    Biochim. Biophys. Acta 1393:179-185(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss Webster / NIH.
  2. "Deletion and alanine mutation analyses for the formation of active homo-or hetero-dimer complexes of mouse choline kinase-alpha and -beta."
    Liao H., Aoyama C., Ishidate K., Teraoka H.
    Biochim. Biophys. Acta 1761:111-120(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  3. "A rostrocaudal muscular dystrophy caused by a defect in choline kinase beta, the first enzyme in phosphatidylcholine biosynthesis."
    Sher R.B., Aoyama C., Huebsch K.A., Ji S., Kerner J., Yang Y., Frankel W.N., Hoppel C.L., Wood P.A., Vance D.E., Cox G.A.
    J. Biol. Chem. 281:4938-4948(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISEASE.
  4. "Understanding the muscular dystrophy caused by deletion of choline kinase beta in mice."
    Wu G., Sher R.B., Cox G.A., Vance D.E.
    Biochim. Biophys. Acta 1791:347-356(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCHKB_MOUSE
AccessioniPrimary (citable) accession number: O55229
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3