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Protein

Choline/ethanolamine kinase

Gene

Chkb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis (By similarity).By similarity

Catalytic activityi

ATP + choline = ADP + phosphocholine.
ATP + ethanolamine = ADP + O-phosphoethanolamine.

Pathwayi: phosphatidylethanolamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Choline/ethanolamine kinase (Chkb), Ethanolamine kinase 2 (Etnk2), Ethanolamine kinase 1 (Etnk1), Choline kinase alpha (Chka)
  2. Ethanolamine-phosphate cytidylyltransferase (Pcyt2)
  3. Choline/ethanolaminephosphotransferase 1 (Cept1), Ethanolaminephosphotransferase 1 (Ept1)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104ATPBy similarity1
Binding sitei244ATPBy similarity1
Binding sitei264ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 81ATPBy similarity7
Nucleotide bindingi146 – 152ATPBy similarity7

GO - Molecular functioni

GO - Biological processi

  • muscle organ development Source: MGI
  • phosphatidylcholine biosynthetic process Source: MGI
  • phosphatidylethanolamine biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1483191. Synthesis of PC.
R-MMU-1483213. Synthesis of PE.
UniPathwayiUPA00558; UER00741.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline/ethanolamine kinase
Alternative name(s):
Choline kinase beta (EC:2.7.1.32)
Short name:
CK
Short name:
CKB
Ethanolamine kinase (EC:2.7.1.82)
Short name:
EK
choline/ethanolamine kinase beta
Short name:
CKEKB
Gene namesi
Name:Chkb
Synonyms:Chetk, Chkl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1328313. Chkb.

Pathology & Biotechi

Involvement in diseasei

Defects in Chkb are a cause of rostrocaudal muscular dystrophy (rmd). The disease is characterized by rapidly progressive muscular dystrophy and neonatal forelimb bone deformity. The dystrophy is only evident in skeletal muscle tissues in an unusual rostral-to-caudal gradient.

Disruption phenotypei

Hindlimb muscular dystrophy. Hindlimb skeletal muscle tissue exhibits impaired phosphatidylcholine biosynthesis and increased phosphatidylcholine catabolism, with concomitant accumulation of choline. Mitochondria are abnormally large and exhibit decreased inner membrane potential.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002062232 – 394Choline/ethanolamine kinaseAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO55229.
PaxDbiO55229.
PeptideAtlasiO55229.
PRIDEiO55229.

PTM databases

iPTMnetiO55229.
PhosphoSitePlusiO55229.

Expressioni

Tissue specificityi

Expressed ubiquitously with the highest level in testis.

Gene expression databases

BgeeiENSMUSG00000022617.
CleanExiMM_CHKB.
ExpressionAtlasiO55229. baseline and differential.
GenevisibleiO55229. MM.

Interactioni

Subunit structurei

Homodimer, and heterodimer with CHKA.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023289.

Structurei

3D structure databases

ProteinModelPortaliO55229.
SMRiO55229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni77 – 79Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the choline/ethanolamine kinase family.Curated

Phylogenomic databases

eggNOGiKOG2686. Eukaryota.
COG0510. LUCA.
GeneTreeiENSGT00530000062991.
HOGENOMiHOG000041274.
HOVERGENiHBG050943.
InParanoidiO55229.
KOiK14156.
OMAiDYEYCAY.
OrthoDBiEOG091G06UK.
PhylomeDBiO55229.
TreeFamiTF313549.

Family and domain databases

InterProiIPR026712. Cho/Etha_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERiPTHR22603:SF35. PTHR22603:SF35. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55229-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAADGTGVVG GGAVGGGLPK DGLQDAKCPE PIPNRRRASS LSRDAQRRAY
60 70 80 90 100
QWCREYLGGA WRRARPEELS VCPVSGGLSN LLFRCSLPNH VPSVGGEPRE
110 120 130 140 150
VLLRLYGAIL QGVDSLVLES VMFAILAERS LGPQLYGVFP EGRLEQYLPS
160 170 180 190 200
RPLKTQELRD PVLSGAIATR MARFHGMEMP FTKEPRWLFG TMERYLKQIQ
210 220 230 240 250
DLPSTSLPQM NLVEMYSLKD EMNSLRKLLD DTPSPVVFCH NDIQEGNILL
260 270 280 290 300
LSEPDSDDNL MLVDFEYSSY NYRGFDIGNH FCEWVYDYTY EEWPFYKARP
310 320 330 340 350
TDYPTREQQL HFIRHYLAEV QKGEILSEEE QKKREEELLL EISRYSLASH
360 370 380 390
FFWGLWSTLQ ASMSTIEFGY LEYAQSRFQF YFQQKGQLTS SPSS
Length:394
Mass (Da):45,126
Last modified:January 23, 2007 - v3
Checksum:iA5EE79EC551D07A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011001 Transcribed RNA. Translation: BAA24897.1.
AB011000 mRNA. Translation: BAA24896.1.
CCDSiCCDS27750.1.
RefSeqiNP_031718.1. NM_007692.6.
UniGeneiMm.485045.

Genome annotation databases

EnsembliENSMUST00000023289; ENSMUSP00000023289; ENSMUSG00000022617.
GeneIDi12651.
KEGGimmu:12651.
UCSCiuc007xgs.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011001 Transcribed RNA. Translation: BAA24897.1.
AB011000 mRNA. Translation: BAA24896.1.
CCDSiCCDS27750.1.
RefSeqiNP_031718.1. NM_007692.6.
UniGeneiMm.485045.

3D structure databases

ProteinModelPortaliO55229.
SMRiO55229.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023289.

PTM databases

iPTMnetiO55229.
PhosphoSitePlusiO55229.

Proteomic databases

EPDiO55229.
PaxDbiO55229.
PeptideAtlasiO55229.
PRIDEiO55229.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023289; ENSMUSP00000023289; ENSMUSG00000022617.
GeneIDi12651.
KEGGimmu:12651.
UCSCiuc007xgs.2. mouse.

Organism-specific databases

CTDi1120.
MGIiMGI:1328313. Chkb.

Phylogenomic databases

eggNOGiKOG2686. Eukaryota.
COG0510. LUCA.
GeneTreeiENSGT00530000062991.
HOGENOMiHOG000041274.
HOVERGENiHBG050943.
InParanoidiO55229.
KOiK14156.
OMAiDYEYCAY.
OrthoDBiEOG091G06UK.
PhylomeDBiO55229.
TreeFamiTF313549.

Enzyme and pathway databases

UniPathwayiUPA00558; UER00741.
ReactomeiR-MMU-1483191. Synthesis of PC.
R-MMU-1483213. Synthesis of PE.

Miscellaneous databases

PROiO55229.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022617.
CleanExiMM_CHKB.
ExpressionAtlasiO55229. baseline and differential.
GenevisibleiO55229. MM.

Family and domain databases

InterProiIPR026712. Cho/Etha_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERiPTHR22603:SF35. PTHR22603:SF35. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCHKB_MOUSE
AccessioniPrimary (citable) accession number: O55229
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.