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O55229

- CHKB_MOUSE

UniProt

O55229 - CHKB_MOUSE

Protein

Choline/ethanolamine kinase

Gene

Chkb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis By similarity.By similarity

    Catalytic activityi

    ATP + choline = ADP + phosphocholine.
    ATP + ethanolamine = ADP + O-phosphoethanolamine.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei104 – 1041ATPBy similarity
    Binding sitei244 – 2441ATPBy similarity
    Binding sitei264 – 2641ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi75 – 817ATPBy similarity
    Nucleotide bindingi146 – 1527ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. choline kinase activity Source: UniProtKB
    3. ethanolamine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. CDP-choline pathway Source: GOC
    2. muscle organ development Source: MGI
    3. phosphatidylcholine biosynthetic process Source: MGI
    4. phosphatidylethanolamine biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_199009. Synthesis of PE.
    REACT_207335. Synthesis of PC.
    UniPathwayiUPA00558; UER00741.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Choline/ethanolamine kinase
    Alternative name(s):
    Choline kinase beta (EC:2.7.1.32)
    Short name:
    CK
    Short name:
    CKB
    Ethanolamine kinase (EC:2.7.1.82)
    Short name:
    EK
    choline/ethanolamine kinase beta
    Short name:
    CKEKB
    Gene namesi
    Name:Chkb
    Synonyms:Chetk, Chkl
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1328313. Chkb.

    Pathology & Biotechi

    Involvement in diseasei

    Defects in Chkb are a cause of rostrocaudal muscular dystrophy (rmd). The disease is characterized by rapidly progressive muscular dystrophy and neonatal forelimb bone deformity. The dystrophy is only evident in skeletal muscle tissues in an unusual rostral-to-caudal gradient.1 Publication

    Disruption phenotypei

    Hindlimb muscular dystrophy. Hindlimb skeletal muscle tissue exhibits impaired phosphatidylcholine biosynthesis and increased phosphatidylcholine catabolism, with concomitant accumulation of choline. Mitochondria are abnormally large and exhibit decreased inner membrane potential.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 394393Choline/ethanolamine kinasePRO_0000206223Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiO55229.
    PaxDbiO55229.
    PRIDEiO55229.

    PTM databases

    PhosphoSiteiO55229.

    Expressioni

    Tissue specificityi

    Expressed ubiquitously with the highest level in testis.

    Gene expression databases

    ArrayExpressiO55229.
    BgeeiO55229.
    CleanExiMM_CHKB.
    GenevestigatoriO55229.

    Interactioni

    Subunit structurei

    Homodimer, and heterodimer with CHKA.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO55229.
    SMRiO55229. Positions 42-390.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni77 – 793Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the choline/ethanolamine kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0510.
    HOGENOMiHOG000041274.
    HOVERGENiHBG050943.
    InParanoidiO55229.
    KOiK14156.
    OMAiFTKEPRW.
    OrthoDBiEOG72VH68.
    PhylomeDBiO55229.
    TreeFamiTF313549.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O55229-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAADGTGVVG GGAVGGGLPK DGLQDAKCPE PIPNRRRASS LSRDAQRRAY    50
    QWCREYLGGA WRRARPEELS VCPVSGGLSN LLFRCSLPNH VPSVGGEPRE 100
    VLLRLYGAIL QGVDSLVLES VMFAILAERS LGPQLYGVFP EGRLEQYLPS 150
    RPLKTQELRD PVLSGAIATR MARFHGMEMP FTKEPRWLFG TMERYLKQIQ 200
    DLPSTSLPQM NLVEMYSLKD EMNSLRKLLD DTPSPVVFCH NDIQEGNILL 250
    LSEPDSDDNL MLVDFEYSSY NYRGFDIGNH FCEWVYDYTY EEWPFYKARP 300
    TDYPTREQQL HFIRHYLAEV QKGEILSEEE QKKREEELLL EISRYSLASH 350
    FFWGLWSTLQ ASMSTIEFGY LEYAQSRFQF YFQQKGQLTS SPSS 394
    Length:394
    Mass (Da):45,126
    Last modified:January 23, 2007 - v3
    Checksum:iA5EE79EC551D07A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB011001 Transcribed RNA. Translation: BAA24897.1.
    AB011000 mRNA. Translation: BAA24896.1.
    CCDSiCCDS27750.1.
    RefSeqiNP_031718.1. NM_007692.6.
    UniGeneiMm.485045.

    Genome annotation databases

    EnsembliENSMUST00000023289; ENSMUSP00000023289; ENSMUSG00000022617.
    GeneIDi12651.
    KEGGimmu:12651.
    UCSCiuc007xgs.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB011001 Transcribed RNA. Translation: BAA24897.1 .
    AB011000 mRNA. Translation: BAA24896.1 .
    CCDSi CCDS27750.1.
    RefSeqi NP_031718.1. NM_007692.6.
    UniGenei Mm.485045.

    3D structure databases

    ProteinModelPortali O55229.
    SMRi O55229. Positions 42-390.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei O55229.

    Proteomic databases

    MaxQBi O55229.
    PaxDbi O55229.
    PRIDEi O55229.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023289 ; ENSMUSP00000023289 ; ENSMUSG00000022617 .
    GeneIDi 12651.
    KEGGi mmu:12651.
    UCSCi uc007xgs.2. mouse.

    Organism-specific databases

    CTDi 1120.
    MGIi MGI:1328313. Chkb.

    Phylogenomic databases

    eggNOGi COG0510.
    HOGENOMi HOG000041274.
    HOVERGENi HBG050943.
    InParanoidi O55229.
    KOi K14156.
    OMAi FTKEPRW.
    OrthoDBi EOG72VH68.
    PhylomeDBi O55229.
    TreeFami TF313549.

    Enzyme and pathway databases

    UniPathwayi UPA00558 ; UER00741 .
    Reactomei REACT_199009. Synthesis of PE.
    REACT_207335. Synthesis of PC.

    Miscellaneous databases

    NextBioi 281860.
    PROi O55229.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O55229.
    Bgeei O55229.
    CleanExi MM_CHKB.
    Genevestigatori O55229.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of mouse choline kinase and choline/ethanolamine kinase: their sequence comparison to the respective rat homologs."
      Aoyama C., Nakashima K., Ishidate K.
      Biochim. Biophys. Acta 1393:179-185(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Swiss Webster / NIH.
    2. "Deletion and alanine mutation analyses for the formation of active homo-or hetero-dimer complexes of mouse choline kinase-alpha and -beta."
      Liao H., Aoyama C., Ishidate K., Teraoka H.
      Biochim. Biophys. Acta 1761:111-120(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    3. "A rostrocaudal muscular dystrophy caused by a defect in choline kinase beta, the first enzyme in phosphatidylcholine biosynthesis."
      Sher R.B., Aoyama C., Huebsch K.A., Ji S., Kerner J., Yang Y., Frankel W.N., Hoppel C.L., Wood P.A., Vance D.E., Cox G.A.
      J. Biol. Chem. 281:4938-4948(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISEASE.
    4. "Understanding the muscular dystrophy caused by deletion of choline kinase beta in mice."
      Wu G., Sher R.B., Cox G.A., Vance D.E.
      Biochim. Biophys. Acta 1791:347-356(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiCHKB_MOUSE
    AccessioniPrimary (citable) accession number: O55229
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 105 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3