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O55229 (CHKB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Choline/ethanolamine kinase
Alternative name(s):
Choline kinase beta
Short name=CK
Short name=CKB
EC=2.7.1.32
Ethanolamine kinase
Short name=EK
EC=2.7.1.82
choline/ethanolamine kinase beta
Short name=CKEKB
Gene names
Name:Chkb
Synonyms:Chetk, Chkl
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis By similarity.

Catalytic activity

ATP + choline = ADP + phosphocholine.

ATP + ethanolamine = ADP + O-phosphoethanolamine.

Pathway

Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.

Subunit structure

Homodimer, and heterodimer with CHKA. Ref.2

Tissue specificity

Expressed ubiquitously with the highest level in testis.

Involvement in disease

Defects in Chkb are a cause of rostrocaudal muscular dystrophy (rmd). The disease is characterized by rapidly progressive muscular dystrophy and neonatal forelimb bone deformity. The dystrophy is only evident in skeletal muscle tissues in an unusual rostral-to-caudal gradient. Ref.3

Disruption phenotype

Hindlimb muscular dystrophy. Hindlimb skeletal muscle tissue exhibits impaired phosphatidylcholine biosynthesis and increased phosphatidylcholine catabolism, with concomitant accumulation of choline. Mitochondria are abnormally large and exhibit decreased inner membrane potential. Ref.4

Sequence similarities

Belongs to the choline/ethanolamine kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 394393Choline/ethanolamine kinase
PRO_0000206223

Regions

Nucleotide binding75 – 817ATP By similarity
Nucleotide binding146 – 1527ATP By similarity
Region77 – 793Substrate binding By similarity

Sites

Binding site1041ATP By similarity
Binding site2441ATP By similarity
Binding site2641ATP By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Sequences

Sequence LengthMass (Da)Tools
O55229 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: A5EE79EC551D07A2

FASTA39445,126
        10         20         30         40         50         60 
MAADGTGVVG GGAVGGGLPK DGLQDAKCPE PIPNRRRASS LSRDAQRRAY QWCREYLGGA 

        70         80         90        100        110        120 
WRRARPEELS VCPVSGGLSN LLFRCSLPNH VPSVGGEPRE VLLRLYGAIL QGVDSLVLES 

       130        140        150        160        170        180 
VMFAILAERS LGPQLYGVFP EGRLEQYLPS RPLKTQELRD PVLSGAIATR MARFHGMEMP 

       190        200        210        220        230        240 
FTKEPRWLFG TMERYLKQIQ DLPSTSLPQM NLVEMYSLKD EMNSLRKLLD DTPSPVVFCH 

       250        260        270        280        290        300 
NDIQEGNILL LSEPDSDDNL MLVDFEYSSY NYRGFDIGNH FCEWVYDYTY EEWPFYKARP 

       310        320        330        340        350        360 
TDYPTREQQL HFIRHYLAEV QKGEILSEEE QKKREEELLL EISRYSLASH FFWGLWSTLQ 

       370        380        390 
ASMSTIEFGY LEYAQSRFQF YFQQKGQLTS SPSS 

« Hide

References

[1]"Molecular cloning of mouse choline kinase and choline/ethanolamine kinase: their sequence comparison to the respective rat homologs."
Aoyama C., Nakashima K., Ishidate K.
Biochim. Biophys. Acta 1393:179-185(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Swiss Webster / NIH.
[2]"Deletion and alanine mutation analyses for the formation of active homo-or hetero-dimer complexes of mouse choline kinase-alpha and -beta."
Liao H., Aoyama C., Ishidate K., Teraoka H.
Biochim. Biophys. Acta 1761:111-120(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[3]"A rostrocaudal muscular dystrophy caused by a defect in choline kinase beta, the first enzyme in phosphatidylcholine biosynthesis."
Sher R.B., Aoyama C., Huebsch K.A., Ji S., Kerner J., Yang Y., Frankel W.N., Hoppel C.L., Wood P.A., Vance D.E., Cox G.A.
J. Biol. Chem. 281:4938-4948(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: DISEASE.
[4]"Understanding the muscular dystrophy caused by deletion of choline kinase beta in mice."
Wu G., Sher R.B., Cox G.A., Vance D.E.
Biochim. Biophys. Acta 1791:347-356(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB011001 Transcribed RNA. Translation: BAA24897.1.
AB011000 mRNA. Translation: BAA24896.1.
CCDSCCDS27750.1.
RefSeqNP_031718.1. NM_007692.6.
UniGeneMm.485045.

3D structure databases

ProteinModelPortalO55229.
SMRO55229. Positions 42-390.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteO55229.

Proteomic databases

MaxQBO55229.
PaxDbO55229.
PRIDEO55229.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023289; ENSMUSP00000023289; ENSMUSG00000022617.
GeneID12651.
KEGGmmu:12651.
UCSCuc007xgs.2. mouse.

Organism-specific databases

CTD1120.
MGIMGI:1328313. Chkb.

Phylogenomic databases

eggNOGCOG0510.
HOGENOMHOG000041274.
HOVERGENHBG050943.
InParanoidO55229.
KOK14156.
OMAFTKEPRW.
OrthoDBEOG72VH68.
PhylomeDBO55229.
TreeFamTF313549.

Enzyme and pathway databases

UniPathwayUPA00558; UER00741.

Gene expression databases

ArrayExpressO55229.
BgeeO55229.
CleanExMM_CHKB.
GenevestigatorO55229.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
ProtoNetSearch...

Other

NextBio281860.
PROO55229.
SOURCESearch...

Entry information

Entry nameCHKB_MOUSE
AccessionPrimary (citable) accession number: O55229
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot