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Protein

Integrin-linked protein kinase

Gene

Ilk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi199 – 207ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • branching involved in ureteric bud morphogenesis Source: MGI
  • cell aging Source: Ensembl
  • cell cycle arrest Source: Ensembl
  • cell projection organization Source: MGI
  • establishment or maintenance of epithelial cell apical/basal polarity Source: MGI
  • extracellular fibril organization Source: Ensembl
  • fibroblast migration Source: MGI
  • integrin-mediated signaling pathway Source: MGI
  • myelin assembly Source: Ensembl
  • myelination in peripheral nervous system Source: MGI
  • negative regulation of cardiac muscle cell apoptotic process Source: Ensembl
  • negative regulation of neural precursor cell proliferation Source: MGI
  • negative regulation of neuron apoptotic process Source: Ensembl
  • negative regulation of protein kinase activity Source: Ensembl
  • negative regulation of smooth muscle cell migration Source: Ensembl
  • negative regulation of smooth muscle cell proliferation Source: Ensembl
  • nerve development Source: MGI
  • neuron projection morphogenesis Source: Ensembl
  • outflow tract morphogenesis Source: CACAO
  • peptidyl-serine phosphorylation Source: MGI
  • platelet aggregation Source: MGI
  • positive regulation of axon extension Source: Ensembl
  • positive regulation of BMP signaling pathway Source: BHF-UCL
  • positive regulation of canonical Wnt signaling pathway Source: MGI
  • positive regulation of cell-matrix adhesion Source: Ensembl
  • positive regulation of cell migration Source: Ensembl
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of dendrite morphogenesis Source: Ensembl
  • positive regulation of MAP kinase activity Source: Ensembl
  • positive regulation of myoblast differentiation Source: Ensembl
  • positive regulation of osteoblast differentiation Source: BHF-UCL
  • positive regulation of phosphorylation Source: MGI
  • positive regulation of protein kinase B signaling Source: Ensembl
  • positive regulation of transcription, DNA-templated Source: MGI
  • protein heterooligomerization Source: Ensembl
  • protein kinase B signaling Source: MGI
  • protein phosphorylation Source: MGI
  • regulation of actin cytoskeleton organization Source: Ensembl
  • Schwann cell development Source: MGI
  • substrate adhesion-dependent cell spreading Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.
ReactomeiR-MMU-446343. Localization of the PINCH-ILK-PARVIN complex to focal adhesions.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin-linked protein kinase (EC:2.7.11.1)
Gene namesi
Name:Ilk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1195267. Ilk.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000860221 – 452Integrin-linked protein kinaseAdd BLAST452

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei186PhosphoserineBy similarity1
Modified residuei426N6-acetyllysineCombined sources1

Post-translational modificationi

Autophosphorylated on serine residues.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO55222.
MaxQBiO55222.
PaxDbiO55222.
PeptideAtlasiO55222.
PRIDEiO55222.

PTM databases

iPTMnetiO55222.
PhosphoSitePlusiO55222.
SwissPalmiO55222.

Expressioni

Tissue specificityi

Highly expressed in lung, heart, kidney, liver, brain, spleen and skeletal muscle. Weakly expressed in testis.

Gene expression databases

BgeeiENSMUSG00000030890.
CleanExiMM_ILK.
ExpressionAtlasiO55222. baseline and differential.
GenevisibleiO55222. MM.

Interactioni

Subunit structurei

Interacts with the cytoplasmic domain of ITGB1. Could also interact with integrin ITGB2, ITGB3 and/or ITGB5. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with PARVA and PARVB. Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1 (By similarity). Interacts with TGFB1I1. Interacts with FERMT2.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LIMS1P480593EBI-6690138,EBI-306928From a different organism.
ParvaQ9EPC14EBI-6690138,EBI-6690233
ParvbQ9ES462EBI-6690138,EBI-6914996
Stub1Q9WUD17EBI-6690138,EBI-773027

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200649. 6 interactors.
DIPiDIP-41971N.
IntActiO55222. 47 interactors.
MINTiMINT-150552.
STRINGi10090.ENSMUSP00000033182.

Structurei

3D structure databases

ProteinModelPortaliO55222.
SMRiO55222.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati2 – 30ANK 1Add BLAST29
Repeati31 – 63ANK 2Add BLAST33
Repeati64 – 96ANK 3Add BLAST33
Repeati97 – 129ANK 4Add BLAST33
Repeati130 – 174ANK 5Add BLAST45
Domaini193 – 446Protein kinasePROSITE-ProRule annotationAdd BLAST254

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 139Interaction with LIMS1By similarityAdd BLAST107
Regioni180 – 212PH-like; mediates interaction with TGFB1I11 PublicationAdd BLAST33

Domaini

A PH-like domain is involved in phosphatidylinositol phosphate binding.By similarity

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0195. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00860000133794.
HOGENOMiHOG000047828.
HOVERGENiHBG002437.
InParanoidiO55222.
KOiK06272.
OMAiMLKVRDW.
OrthoDBiEOG091G05IK.
PhylomeDBiO55222.
TreeFamiTF315194.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O55222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV
60 70 80 90 100
EMLIMRGARI NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG
110 120 130 140 150
NVPLHYACFW GQDQVAEDLV ANGALVSICN KYGEMPVDKA KAPLRELLRE
160 170 180 190 200
RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG TLNKHSGIDF KQLNFLAKLN
210 220 230 240 250
ENHSGELWKG RWQGNDIVVK VLKVRDWSTR KSRDFNEECP RLRIFSHPNV
260 270 280 290 300
LPVLGACQAP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD
310 320 330 340 350
MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM
360 370 380 390 400
YAPAWVAPEA LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI
410 420 430 440 450
GMKVALEGLR PTIPPGISPH VCKLMKICMN EDPAKRPKFD MIVPILEKMQ

DK
Length:452
Mass (Da):51,373
Last modified:October 31, 2006 - v2
Checksum:iF41960CF8EC503A7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti93I → T in AAB94646 (PubMed:9366252).Curated1
Sequence conflicti413I → V in AAB94646 (PubMed:9366252).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94479 mRNA. Translation: AAB94646.1.
BC003737 mRNA. Translation: AAH03737.1.
CCDSiCCDS21659.1.
RefSeqiNP_001155196.1. NM_001161724.1.
NP_034692.2. NM_010562.2.
UniGeneiMm.274846.

Genome annotation databases

EnsembliENSMUST00000033182; ENSMUSP00000033182; ENSMUSG00000030890.
ENSMUST00000163389; ENSMUSP00000130341; ENSMUSG00000030890.
GeneIDi16202.
KEGGimmu:16202.
UCSCiuc009izb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94479 mRNA. Translation: AAB94646.1.
BC003737 mRNA. Translation: AAH03737.1.
CCDSiCCDS21659.1.
RefSeqiNP_001155196.1. NM_001161724.1.
NP_034692.2. NM_010562.2.
UniGeneiMm.274846.

3D structure databases

ProteinModelPortaliO55222.
SMRiO55222.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200649. 6 interactors.
DIPiDIP-41971N.
IntActiO55222. 47 interactors.
MINTiMINT-150552.
STRINGi10090.ENSMUSP00000033182.

PTM databases

iPTMnetiO55222.
PhosphoSitePlusiO55222.
SwissPalmiO55222.

Proteomic databases

EPDiO55222.
MaxQBiO55222.
PaxDbiO55222.
PeptideAtlasiO55222.
PRIDEiO55222.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033182; ENSMUSP00000033182; ENSMUSG00000030890.
ENSMUST00000163389; ENSMUSP00000130341; ENSMUSG00000030890.
GeneIDi16202.
KEGGimmu:16202.
UCSCiuc009izb.2. mouse.

Organism-specific databases

CTDi3611.
MGIiMGI:1195267. Ilk.

Phylogenomic databases

eggNOGiKOG0195. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00860000133794.
HOGENOMiHOG000047828.
HOVERGENiHBG002437.
InParanoidiO55222.
KOiK06272.
OMAiMLKVRDW.
OrthoDBiEOG091G05IK.
PhylomeDBiO55222.
TreeFamiTF315194.

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.
ReactomeiR-MMU-446343. Localization of the PINCH-ILK-PARVIN complex to focal adhesions.

Miscellaneous databases

PROiO55222.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030890.
CleanExiMM_ILK.
ExpressionAtlasiO55222. baseline and differential.
GenevisibleiO55222. MM.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiILK_MOUSE
AccessioniPrimary (citable) accession number: O55222
Secondary accession number(s): Q78KK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 31, 2006
Last modified: November 30, 2016
This is version 163 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.