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O55222

- ILK_MOUSE

UniProt

O55222 - ILK_MOUSE

Protein

Integrin-linked protein kinase

Gene

Ilk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 2 (31 Oct 2006)
      Previous versions | rss
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    Functioni

    Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei220 – 2201ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi199 – 2079ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine kinase activity Source: MGI
    4. signal transducer activity Source: Ensembl

    GO - Biological processi

    1. branching involved in ureteric bud morphogenesis Source: MGI
    2. cell aging Source: Ensembl
    3. cell cycle arrest Source: Ensembl
    4. cell projection organization Source: MGI
    5. establishment or maintenance of epithelial cell apical/basal polarity Source: MGI
    6. extracellular fibril organization Source: Ensembl
    7. fibroblast migration Source: MGI
    8. integrin-mediated signaling pathway Source: MGI
    9. myelin assembly Source: Ensembl
    10. myelination in peripheral nervous system Source: MGI
    11. negative regulation of cardiac muscle cell apoptotic process Source: Ensembl
    12. negative regulation of neural precursor cell proliferation Source: MGI
    13. negative regulation of neuron apoptotic process Source: Ensembl
    14. negative regulation of protein kinase activity Source: Ensembl
    15. negative regulation of smooth muscle cell migration Source: Ensembl
    16. negative regulation of smooth muscle cell proliferation Source: Ensembl
    17. nerve development Source: MGI
    18. neuron projection morphogenesis Source: Ensembl
    19. peptidyl-serine phosphorylation Source: MGI
    20. positive regulation of axon extension Source: Ensembl
    21. positive regulation of BMP signaling pathway Source: BHF-UCL
    22. positive regulation of canonical Wnt signaling pathway Source: Ensembl
    23. positive regulation of cell-matrix adhesion Source: Ensembl
    24. positive regulation of cell migration Source: Ensembl
    25. positive regulation of cell proliferation Source: MGI
    26. positive regulation of dendrite morphogenesis Source: Ensembl
    27. positive regulation of MAP kinase activity Source: Ensembl
    28. positive regulation of myoblast differentiation Source: Ensembl
    29. positive regulation of osteoblast differentiation Source: BHF-UCL
    30. positive regulation of protein kinase B signaling Source: Ensembl
    31. positive regulation of transcription, DNA-templated Source: Ensembl
    32. protein heterooligomerization Source: Ensembl
    33. protein kinase B signaling Source: MGI
    34. regulation of actin cytoskeleton organization Source: Ensembl
    35. Schwann cell development Source: MGI
    36. substrate adhesion-dependent cell spreading Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.10.2. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Integrin-linked protein kinase (EC:2.7.11.1)
    Gene namesi
    Name:Ilk
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1195267. Ilk.

    Subcellular locationi

    GO - Cellular componenti

    1. cell-cell junction Source: Ensembl
    2. costamere Source: Ensembl
    3. dendritic shaft Source: Ensembl
    4. focal adhesion Source: MGI
    5. lamellipodium Source: UniProtKB
    6. neuronal cell body Source: Ensembl
    7. plasma membrane Source: UniProtKB-SubCell
    8. protein complex Source: Ensembl
    9. sarcomere Source: UniProtKB-SubCell
    10. terminal bouton Source: Ensembl

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 452452Integrin-linked protein kinasePRO_0000086022Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei186 – 1861PhosphoserineBy similarity
    Modified residuei426 – 4261N6-acetyllysine1 Publication

    Post-translational modificationi

    Autophosphorylated on serine residues.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO55222.
    PaxDbiO55222.
    PRIDEiO55222.

    PTM databases

    PhosphoSiteiO55222.

    Expressioni

    Tissue specificityi

    Highly expressed in lung, heart, kidney, liver, brain, spleen and skeletal muscle. Weakly expressed in testis.

    Gene expression databases

    ArrayExpressiO55222.
    BgeeiO55222.
    CleanExiMM_ILK.
    GenevestigatoriO55222.

    Interactioni

    Subunit structurei

    Interacts with the cytoplasmic domain of ITGB1. Could also interact with integrin ITGB2, ITGB3 and/or ITGB5. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with PARVA and PARVB. Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1 By similarity. Interacts with TGFB1I1. Interacts with FERMT2.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LIMS1P480593EBI-6690138,EBI-306928From a different organism.
    ParvaQ9EPC14EBI-6690138,EBI-6690233
    ParvbQ9ES462EBI-6690138,EBI-6914996
    Stub1Q9WUD17EBI-6690138,EBI-773027

    Protein-protein interaction databases

    BioGridi200649. 6 interactions.
    DIPiDIP-41971N.
    IntActiO55222. 44 interactions.
    MINTiMINT-150552.
    STRINGi10090.ENSMUSP00000033182.

    Structurei

    3D structure databases

    ProteinModelPortaliO55222.
    SMRiO55222. Positions 2-171, 185-452.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati2 – 3029ANK 1Add
    BLAST
    Repeati31 – 6333ANK 2Add
    BLAST
    Repeati64 – 9633ANK 3Add
    BLAST
    Repeati97 – 12933ANK 4Add
    BLAST
    Repeati130 – 17445ANK 5Add
    BLAST
    Domaini193 – 446254Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni33 – 139107Interaction with LIMS1By similarityAdd
    BLAST
    Regioni180 – 21233PH-like; mediates interaction with TGFB1I1Add
    BLAST

    Domaini

    A PH-like domain is involved in phosphatidylinositol phosphate binding.By similarity

    Sequence similaritiesi

    Contains 5 ANK repeats.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat

    Phylogenomic databases

    eggNOGiCOG0666.
    GeneTreeiENSGT00750000117425.
    HOGENOMiHOG000047828.
    HOVERGENiHBG002437.
    InParanoidiO55222.
    KOiK06272.
    OMAiMLKVRDW.
    OrthoDBiEOG7T1RB0.
    PhylomeDBiO55222.
    TreeFamiTF315194.

    Family and domain databases

    Gene3Di1.25.40.20. 2 hits.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR016253. Integrin-linked_kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF00023. Ank. 3 hits.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000654. Integrin-linked_kinase. 1 hit.
    SMARTiSM00248. ANK. 3 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 3 hits.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O55222-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV    50
    EMLIMRGARI NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG 100
    NVPLHYACFW GQDQVAEDLV ANGALVSICN KYGEMPVDKA KAPLRELLRE 150
    RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG TLNKHSGIDF KQLNFLAKLN 200
    ENHSGELWKG RWQGNDIVVK VLKVRDWSTR KSRDFNEECP RLRIFSHPNV 250
    LPVLGACQAP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD 300
    MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM 350
    YAPAWVAPEA LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI 400
    GMKVALEGLR PTIPPGISPH VCKLMKICMN EDPAKRPKFD MIVPILEKMQ 450
    DK 452
    Length:452
    Mass (Da):51,373
    Last modified:October 31, 2006 - v2
    Checksum:iF41960CF8EC503A7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti93 – 931I → T in AAB94646. (PubMed:9366252)Curated
    Sequence conflicti413 – 4131I → V in AAB94646. (PubMed:9366252)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U94479 mRNA. Translation: AAB94646.1.
    BC003737 mRNA. Translation: AAH03737.1.
    CCDSiCCDS21659.1.
    RefSeqiNP_001155196.1. NM_001161724.1.
    NP_034692.2. NM_010562.2.
    UniGeneiMm.274846.

    Genome annotation databases

    EnsembliENSMUST00000033182; ENSMUSP00000033182; ENSMUSG00000030890.
    ENSMUST00000163389; ENSMUSP00000130341; ENSMUSG00000030890.
    GeneIDi16202.
    KEGGimmu:16202.
    UCSCiuc009izb.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U94479 mRNA. Translation: AAB94646.1 .
    BC003737 mRNA. Translation: AAH03737.1 .
    CCDSi CCDS21659.1.
    RefSeqi NP_001155196.1. NM_001161724.1.
    NP_034692.2. NM_010562.2.
    UniGenei Mm.274846.

    3D structure databases

    ProteinModelPortali O55222.
    SMRi O55222. Positions 2-171, 185-452.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200649. 6 interactions.
    DIPi DIP-41971N.
    IntActi O55222. 44 interactions.
    MINTi MINT-150552.
    STRINGi 10090.ENSMUSP00000033182.

    PTM databases

    PhosphoSitei O55222.

    Proteomic databases

    MaxQBi O55222.
    PaxDbi O55222.
    PRIDEi O55222.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033182 ; ENSMUSP00000033182 ; ENSMUSG00000030890 .
    ENSMUST00000163389 ; ENSMUSP00000130341 ; ENSMUSG00000030890 .
    GeneIDi 16202.
    KEGGi mmu:16202.
    UCSCi uc009izb.2. mouse.

    Organism-specific databases

    CTDi 3611.
    MGIi MGI:1195267. Ilk.

    Phylogenomic databases

    eggNOGi COG0666.
    GeneTreei ENSGT00750000117425.
    HOGENOMi HOG000047828.
    HOVERGENi HBG002437.
    InParanoidi O55222.
    KOi K06272.
    OMAi MLKVRDW.
    OrthoDBi EOG7T1RB0.
    PhylomeDBi O55222.
    TreeFami TF315194.

    Enzyme and pathway databases

    BRENDAi 2.7.10.2. 3474.

    Miscellaneous databases

    NextBioi 289147.
    PROi O55222.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O55222.
    Bgeei O55222.
    CleanExi MM_ILK.
    Genevestigatori O55222.

    Family and domain databases

    Gene3Di 1.25.40.20. 2 hits.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR016253. Integrin-linked_kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF00023. Ank. 3 hits.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000654. Integrin-linked_kinase. 1 hit.
    SMARTi SM00248. ANK. 3 hits.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 3 hits.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of a mouse protein kinase that is highly homologous to human integrin-linked kinase."
      Li F., Liu J., Mayne R., Wu C.
      Biochim. Biophys. Acta 1358:215-220(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Heart.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    3. "Oligomerizing potential of a focal adhesion LIM protein Hic-5 organizing a nuclear-cytoplasmic shuttling complex."
      Mori K., Asakawa M., Hayashi M., Imura M., Ohki T., Hirao E., Kim-Kaneyama J.-R., Nose K., Shibanuma M.
      J. Biol. Chem. 281:22048-22061(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TGFB1I1.
    4. Cited for: SUBCELLULAR LOCATION.
    5. "Kindlin-2 controls bidirectional signaling of integrins."
      Montanez E., Ussar S., Schifferer M., Bosl M., Zent R., Moser M., Fassler R.
      Genes Dev. 22:1325-1330(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH FERMT2.
    6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-426, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiILK_MOUSE
    AccessioniPrimary (citable) accession number: O55222
    Secondary accession number(s): Q78KK2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: October 31, 2006
    Last modified: October 1, 2014
    This is version 139 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3