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O55222 (ILK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Integrin-linked protein kinase

EC=2.7.11.1
Gene names
Name:Ilk
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length452 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with the cytoplasmic domain of ITGB1. Could also interact with integrin ITGB2, ITGB3 and/or ITGB5. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with PARVA and PARVB. Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1 By similarity. Interacts with TGFB1I1. Interacts with FERMT2. Ref.3 Ref.5

Subcellular location

Cell junctionfocal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasmmyofibrilsarcomere By similarity. Cell projectionlamellipodium Ref.4 Ref.5.

Tissue specificity

Highly expressed in lung, heart, kidney, liver, brain, spleen and skeletal muscle. Weakly expressed in testis.

Domain

A PH-like domain is involved in phosphatidylinositol phosphate binding By similarity.

Post-translational modification

Autophosphorylated on serine residues By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Contains 5 ANK repeats.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Cellular componentCell junction
Cell membrane
Cell projection
Cytoplasm
Membrane
   DomainANK repeat
Repeat
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSchwann cell development

Inferred from mutant phenotype PubMed 19349584. Source: MGI

branching involved in ureteric bud morphogenesis

Inferred from direct assay PubMed 15831470. Source: MGI

cell aging

Inferred from electronic annotation. Source: Ensembl

cell cycle arrest

Inferred from electronic annotation. Source: Ensembl

cell projection organization

Inferred from mutant phenotype PubMed 19349584. Source: MGI

establishment or maintenance of epithelial cell apical/basal polarity

Inferred from mutant phenotype PubMed 12670870. Source: MGI

extracellular fibril organization

Inferred from electronic annotation. Source: Ensembl

fibroblast migration

Inferred from mutant phenotype PubMed 23658024. Source: MGI

integrin-mediated signaling pathway

Inferred from sequence orthology Ref.1. Source: MGI

myelin assembly

Inferred from electronic annotation. Source: Ensembl

myelination in peripheral nervous system

Inferred from mutant phenotype PubMed 19349584. Source: MGI

negative regulation of cardiac muscle cell apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of neuron apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of protein kinase activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of smooth muscle cell migration

Inferred from electronic annotation. Source: Ensembl

negative regulation of smooth muscle cell proliferation

Inferred from electronic annotation. Source: Ensembl

nerve development

Inferred from mutant phenotype PubMed 19349584. Source: MGI

neuron projection morphogenesis

Inferred from electronic annotation. Source: Ensembl

peptidyl-serine phosphorylation

Inferred from mutant phenotype PubMed 19349584. Source: MGI

positive regulation of BMP signaling pathway

Inferred from mutant phenotype PubMed 20675382. Source: BHF-UCL

positive regulation of MAP kinase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of axon extension

Inferred from electronic annotation. Source: Ensembl

positive regulation of canonical Wnt signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell migration

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 12835312. Source: MGI

positive regulation of cell-matrix adhesion

Inferred from electronic annotation. Source: Ensembl

positive regulation of dendrite morphogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of myoblast differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of osteoblast differentiation

Inferred from mutant phenotype PubMed 20675382. Source: BHF-UCL

positive regulation of protein kinase B signaling

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

protein heterooligomerization

Inferred from electronic annotation. Source: Ensembl

protein kinase B signaling

Inferred from mutant phenotype PubMed 19349584. Source: MGI

regulation of actin cytoskeleton organization

Inferred from electronic annotation. Source: Ensembl

substrate adhesion-dependent cell spreading

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell-cell junction

Inferred from electronic annotation. Source: Ensembl

costamere

Inferred from electronic annotation. Source: Ensembl

dendritic shaft

Inferred from electronic annotation. Source: Ensembl

focal adhesion

Inferred from direct assay PubMed 12432066PubMed 15565145PubMed 23658024. Source: MGI

lamellipodium

Inferred from direct assay Ref.4. Source: UniProtKB

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

protein complex

Inferred from electronic annotation. Source: Ensembl

sarcomere

Inferred from electronic annotation. Source: UniProtKB-SubCell

terminal bouton

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 10574708PubMed 12432066PubMed 19798050PubMed 23612611. Source: IntAct

protein serine/threonine kinase activity

Inferred from sequence orthology Ref.1. Source: MGI

signal transducer activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 452452Integrin-linked protein kinase
PRO_0000086022

Regions

Repeat2 – 3029ANK 1
Repeat31 – 6333ANK 2
Repeat64 – 9633ANK 3
Repeat97 – 12933ANK 4
Repeat130 – 17445ANK 5
Domain193 – 446254Protein kinase
Nucleotide binding199 – 2079ATP By similarity
Region33 – 139107Interaction with LIMS1 By similarity
Region180 – 21233PH-like; mediates interaction with TGFB1I1

Sites

Binding site2201ATP By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue1861Phosphoserine By similarity
Modified residue4261N6-acetyllysine Ref.6

Experimental info

Sequence conflict931I → T in AAB94646. Ref.1
Sequence conflict4131I → V in AAB94646. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O55222 [UniParc].

Last modified October 31, 2006. Version 2.
Checksum: F41960CF8EC503A7

FASTA45251,373
        10         20         30         40         50         60 
MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV EMLIMRGARI 

        70         80         90        100        110        120 
NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG NVPLHYACFW GQDQVAEDLV 

       130        140        150        160        170        180 
ANGALVSICN KYGEMPVDKA KAPLRELLRE RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG 

       190        200        210        220        230        240 
TLNKHSGIDF KQLNFLAKLN ENHSGELWKG RWQGNDIVVK VLKVRDWSTR KSRDFNEECP 

       250        260        270        280        290        300 
RLRIFSHPNV LPVLGACQAP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD 

       310        320        330        340        350        360 
MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM YAPAWVAPEA 

       370        380        390        400        410        420 
LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI GMKVALEGLR PTIPPGISPH 

       430        440        450 
VCKLMKICMN EDPAKRPKFD MIVPILEKMQ DK 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of a mouse protein kinase that is highly homologous to human integrin-linked kinase."
Li F., Liu J., Mayne R., Wu C.
Biochim. Biophys. Acta 1358:215-220(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Heart.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[3]"Oligomerizing potential of a focal adhesion LIM protein Hic-5 organizing a nuclear-cytoplasmic shuttling complex."
Mori K., Asakawa M., Hayashi M., Imura M., Ohki T., Hirao E., Kim-Kaneyama J.-R., Nose K., Shibanuma M.
J. Biol. Chem. 281:22048-22061(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TGFB1I1.
[4]"Affixin activates Rac1 via betaPIX in C2C12 myoblast."
Matsuda C., Kameyama K., Suzuki A., Mishima W., Yamaji S., Okamoto H., Nishino I., Hayashi Y.K.
FEBS Lett. 582:1189-1196(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"Kindlin-2 controls bidirectional signaling of integrins."
Montanez E., Ussar S., Schifferer M., Bosl M., Zent R., Moser M., Fassler R.
Genes Dev. 22:1325-1330(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH FERMT2.
[6]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-426, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U94479 mRNA. Translation: AAB94646.1.
BC003737 mRNA. Translation: AAH03737.1.
CCDSCCDS21659.1.
RefSeqNP_001155196.1. NM_001161724.1.
NP_034692.2. NM_010562.2.
UniGeneMm.274846.

3D structure databases

ProteinModelPortalO55222.
SMRO55222. Positions 2-171, 185-452.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200649. 6 interactions.
DIPDIP-41971N.
IntActO55222. 44 interactions.
MINTMINT-150552.
STRING10090.ENSMUSP00000033182.

PTM databases

PhosphoSiteO55222.

Proteomic databases

MaxQBO55222.
PaxDbO55222.
PRIDEO55222.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033182; ENSMUSP00000033182; ENSMUSG00000030890.
ENSMUST00000163389; ENSMUSP00000130341; ENSMUSG00000030890.
GeneID16202.
KEGGmmu:16202.
UCSCuc009izb.2. mouse.

Organism-specific databases

CTD3611.
MGIMGI:1195267. Ilk.

Phylogenomic databases

eggNOGCOG0666.
GeneTreeENSGT00750000117425.
HOGENOMHOG000047828.
HOVERGENHBG002437.
InParanoidO55222.
KOK06272.
OMAMLKVRDW.
OrthoDBEOG7T1RB0.
PhylomeDBO55222.
TreeFamTF315194.

Enzyme and pathway databases

BRENDA2.7.10.2. 3474.

Gene expression databases

ArrayExpressO55222.
BgeeO55222.
CleanExMM_ILK.
GenevestigatorO55222.

Family and domain databases

Gene3D1.25.40.20. 2 hits.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR016253. Integrin-linked_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamPF00023. Ank. 3 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PIRSFPIRSF000654. Integrin-linked_kinase. 1 hit.
SMARTSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio289147.
PROO55222.
SOURCESearch...

Entry information

Entry nameILK_MOUSE
AccessionPrimary (citable) accession number: O55222
Secondary accession number(s): Q78KK2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 31, 2006
Last modified: July 9, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot