ID   FIGLA_MOUSE             Reviewed;         194 AA.
AC   O55208;
DT   16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   24-JAN-2024, entry version 150.
DE   RecName: Full=Factor in the germline alpha;
DE            Short=FIGalpha;
DE   AltName: Full=Transcription factor FIGa;
GN   Name=Figla;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Ovary;
RX   PubMed=9362457; DOI=10.1242/dev.124.24.4939;
RA   Liang L.-F., Soyal S.M., Dean J.;
RT   "FIGalpha, a germ cell specific transcription factor involved in the
RT   coordinate expression of the zona pellucida genes.";
RL   Development 124:4939-4947(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, FUNCTION, AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=11023867; DOI=10.1242/dev.127.21.4645;
RA   Soyal S.M., Amleh A., Dean J.;
RT   "FIGalpha, a germ cell-specific transcription factor required for ovarian
RT   follicle formation.";
RL   Development 127:4645-4654(2000).
CC   -!- FUNCTION: Germ-line specific transcription factor implicated in
CC       postnatal oocyte-specific gene expression. Plays a key regulatory role
CC       in the expression of multiple oocyte-specific genes, including those
CC       that initiate folliculogenesis and those that encode the zona pellucida
CC       (ZP1, ZP2 and ZP3) required for fertilization and early embryonic
CC       survival. Essential for oocytes to survive and form primordial
CC       follicles. The persistence of FIGLA in adult females suggests that it
CC       may regulate additional pathways that are essential for normal ovarian
CC       development. Binds to the E-box (5'-CANNTG-3') of the ZPs (ZP1, ZP2,
CC       ZP3) promoters. {ECO:0000269|PubMed:11023867}.
CC   -!- SUBUNIT: Heterodimer with TCF3/isoform E12.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed only in the oocytes within the ovary and
CC       at lower level in the testis. Found in the resting oocytes of the
CC       primordial follicle cells, at the periphery of the ovary and in the
CC       hilar region. Also detected in growing oocytes, but at lower levels.
CC   -!- DEVELOPMENTAL STAGE: Detected as early as embryonic day 13, shortly
CC       after the onset of sexual dimorphism of the gonads. Dramatically
CC       increases at the end of the embryonic development and peaks at 2 dpp, a
CC       time in ovarian development at which oocytes have become enclosed in
CC       primordial follicles. Decreases markedly by 7 and 14 days after birth.
CC       Persists, but at low level, in adult females. Not detected in male
CC       embryos, but detected in adult testes. {ECO:0000269|PubMed:11023867}.
CC   -!- DISRUPTION PHENOTYPE: Females display a defect in the formation of
CC       primordial follicles leading to infertility. Although embryonic
CC       gonadogenesis appeared normal, primordial follicles were not formed at
CC       birth, and massive depletion of oocytes resulted in shrunken ovaries
CC       and female sterility. Null females do not express ZP1, ZP2 or ZP3.
CC       Since its expression is oocyte-specific in females, Figla is a
CC       plausible candidate gene for primary ovarian failure in otherwise
CC       phenotypically normal women. The gene for Figla null males have normal
CC       fertility. {ECO:0000269|PubMed:11023867}.
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DR   EMBL; U91840; AAB97139.1; -; mRNA.
DR   CCDS; CCDS20307.1; -.
DR   RefSeq; NP_036143.1; NM_012013.2.
DR   AlphaFoldDB; O55208; -.
DR   SMR; O55208; -.
DR   STRING; 10090.ENSMUSP00000032070; -.
DR   PhosphoSitePlus; O55208; -.
DR   PaxDb; 10090-ENSMUSP00000032070; -.
DR   Antibodypedia; 31154; 88 antibodies from 19 providers.
DR   DNASU; 26910; -.
DR   Ensembl; ENSMUST00000032070.4; ENSMUSP00000032070.4; ENSMUSG00000030001.5.
DR   GeneID; 26910; -.
DR   KEGG; mmu:26910; -.
DR   UCSC; uc009crb.1; mouse.
DR   AGR; MGI:1349421; -.
DR   CTD; 344018; -.
DR   MGI; MGI:1349421; Figla.
DR   VEuPathDB; HostDB:ENSMUSG00000030001; -.
DR   eggNOG; KOG4029; Eukaryota.
DR   GeneTree; ENSGT00440000033552; -.
DR   HOGENOM; CLU_1380972_0_0_1; -.
DR   InParanoid; O55208; -.
DR   OMA; PDKQNYS; -.
DR   OrthoDB; 5360659at2759; -.
DR   PhylomeDB; O55208; -.
DR   TreeFam; TF351992; -.
DR   BioGRID-ORCS; 26910; 2 hits in 75 CRISPR screens.
DR   PRO; PR:O55208; -.
DR   Proteomes; UP000000589; Chromosome 6.
DR   RNAct; O55208; Protein.
DR   Bgee; ENSMUSG00000030001; Expressed in primary oocyte and 17 other cell types or tissues.
DR   ExpressionAtlas; O55208; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005667; C:transcription regulator complex; ISS:UniProtKB.
DR   GO; GO:0043425; F:bHLH transcription factor binding; ISS:UniProtKB.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0070888; F:E-box binding; IEA:Ensembl.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR   GO; GO:0032502; P:developmental process; IBA:GO_Central.
DR   GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd11422; bHLH_TS_FIGLA; 1.
DR   Gene3D; 4.10.280.10; Helix-loop-helix DNA-binding domain; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   PANTHER; PTHR23349; BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST; 1.
DR   PANTHER; PTHR23349:SF57; FACTOR IN THE GERMLINE ALPHA; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; HLH, helix-loop-helix DNA-binding domain; 1.
DR   PROSITE; PS50888; BHLH; 1.
DR   Genevisible; O55208; MM.
PE   2: Evidence at transcript level;
KW   Developmental protein; Differentiation; DNA-binding; Nucleus; Oogenesis;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..194
FT                   /note="Factor in the germline alpha"
FT                   /id="PRO_0000127180"
FT   DOMAIN          59..111
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          121..163
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        135..158
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   194 AA;  21529 MW;  B12137E37AF22723 CRC64;
     MDTAPASPEP FLVTPQAEVL EELIQAQMGP LPRLAAICRL KRLPSGGYST TDDLHLVLER
     RRVANAKERE RIKNLNRGFA KLKALVPFLP QSRKPSKVDI LKGATEYIQI LGCVLEEAKV
     SEKQSPEEQT HSGRPSDPHV SSTRELLGNA TQPTSCASGL KKEEEGPWAY AGHSEPLYSY
     HQSTVPETRS YFTH
//