O55208 (FIGLA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Factor in the germline alpha
Short name=FIGalpha

Alternative name(s):
Transcription factor FIGa

Gene names

Name:

Figla

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Germ-line specific transcription factor implicated in postnatal oocyte-specific gene expression. Plays a key regulatory role in the expression of multiple oocyte-specific genes, including those that initiate folliculogenesis and those that encode the zona pellucida (ZP1, ZP2 and ZP3) required for fertilization and early embryonic survival. Essential for oocytes to survive and form primordial follicles. The persistence of FIGLA in adult females suggests that it may regulate additional pathways that are essential for normal ovarian development. Binds to the E-box (5'-CANNTG-3') of the ZPs (ZP1, ZP2, ZP3) promoters. Ref.2
Subunit structure	Heterodimer with TCF3/isoform E12.
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed only in the oocytes within the ovary and at lower level in the testis. Found in the resting oocytes of the primordial follicle cells, at the periphery of the ovary and in the hilar region. Also detected in growing oocytes, but at lower levels.
Developmental stage	Detected as early as embryonic day 13, shortly after the onset of sexual dimorphism of the gonads. Dramatically increases at the end of the embryonic development and peaks at 2 dpp, a time in ovarian development at which oocytes have become enclosed in primordial follicles. Decreases markedly by 7 and 14 days after birth. Persists, but at low level, in adult females. Not detected in male embryos, but detected in adult testes. Ref.2
Disruption phenotype	Females display a defect in the formation of primordial follicles leading to infertility. Although embryonic gonadogenesis appeared normal, primordial follicles were not formed at birth, and massive depletion of oocytes resulted in shrunken ovaries and female sterility. Null females do not express ZP1, ZP2 or ZP3. Since its expression is oocyte-specific in females, Figla is a plausible candidate gene for primary ovarian failure in otherwise phenotypically normal women. The gene for Figla null males have normal fertility. Ref.2
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
Biological process	Differentiation Oogenesis Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW oogenesis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	transcription factor complex Inferred from sequence or structural similarity PubMed 15044608. Source: UniProtKB
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: Compara transcription factor binding Inferred from sequence or structural similarity PubMed 15044608. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Factor in the germline alpha		PRO_0000127180
Regions
	Domain	59 – 111	53	bHLH

Sequences

	Sequence		Length	Mass (Da)
	O55208 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: B12137E37AF22723	FASTA	194	21,529
	10 20 30 40 50 60 MDTAPASPEP FLVTPQAEVL EELIQAQMGP LPRLAAICRL KRLPSGGYST TDDLHLVLER 70 80 90 100 110 120 RRVANAKERE RIKNLNRGFA KLKALVPFLP QSRKPSKVDI LKGATEYIQI LGCVLEEAKV 130 140 150 160 170 180 SEKQSPEEQT HSGRPSDPHV SSTRELLGNA TQPTSCASGL KKEEEGPWAY AGHSEPLYSY 190 HQSTVPETRS YFTH

References

[1]	"FIGalpha, a germ cell specific transcription factor involved in the coordinate expression of the zona pellucida genes." Liang L.-F., Soyal S.M., Dean J. Development 124:4939-4947(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.
[2]	"FIGalpha, a germ cell-specific transcription factor required for ovarian follicle formation." Soyal S.M., Amleh A., Dean J. Development 127:4645-4654(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, FUNCTION, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U91840 mRNA. Translation: AAB97139.1.
IPI	IPI00116617.
RefSeq	NP_036143.1. NM_012013.1.
UniGene	Mm.8024.
3D structure databases
ProteinModelPortal	O55208.
SMR	O55208. Positions 72-117.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000032070.
PTM databases
PhosphoSite	O55208.
Proteomic databases
PRIDE	O55208.
Protocols and materials databases
DNASU	26910.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032070; ENSMUSP00000032070; ENSMUSG00000030001.
GeneID	26910.
KEGG	mmu:26910.
UCSC	uc009crb.1. mouse.
Organism-specific databases
CTD	344018.
MGI	MGI:1349421. Figla.
Phylogenomic databases
eggNOG	NOG316826.
GeneTree	ENSGT00440000033552.
HOGENOM	HOG000112589.
HOVERGEN	HBG051616.
InParanoid	O55208.
KO	K09066.
OMA	EEGPWAD.
OrthoDB	EOG4GTKDZ.
Gene expression databases
ArrayExpress	O55208.
Bgee	O55208.
CleanEx	MM_FIGLA.
Genevestigator	O55208.
GermOnline	ENSMUSG00000030001. Mus musculus.
Family and domain databases
Gene3D	4.10.280.10. 1 hit.
InterPro	IPR011598. bHLH_dom. [Graphical view]
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. BHLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	304781.
SOURCE	Search...

Entry information

Entry name

FIGLA_MOUSE

Accession

Primary (citable) accession number: O55208

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program