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Protein

Synaptojanin-2

Gene

Synj2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inositol 5-phosphatase which may be involved in distinct membrane trafficking and signal transduction pathways. May mediate the inhibitory effect of Rac1 on endocytosis (By similarity).By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.By similarity

GO - Molecular functioni

  • inositol-polyphosphate 5-phosphatase activity Source: RGD
  • nucleotide binding Source: InterPro
  • PDZ domain binding Source: RGD
  • phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Source: UniProtKB-EC
  • phosphatidylinositol monophosphate phosphatase activity Source: RGD
  • RNA binding Source: UniProtKB-KW
  • SH3 domain binding Source: RGD

GO - Biological processi

  • brain development Source: RGD
  • inositol phosphate dephosphorylation Source: RGD
  • intracellular distribution of mitochondria Source: RGD
  • phosphatidylinositol dephosphorylation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptojanin-2 (EC:3.1.3.36By similarity)
Alternative name(s):
Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2
Gene namesi
Name:Synj2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69436. Synj2.

Subcellular locationi

Isoform 2A :
  • Mitochondrion 1 Publication

  • Note: Interaction of isoform 2A with SYNJ2BP/OMP25 results in localization to the mitochondrion (PubMed:10357812).1 Publication

GO - Cellular componenti

  • axon terminus Source: RGD
  • cytoplasm Source: RGD
  • cytoplasmic microtubule Source: RGD
  • extrinsic component of mitochondrial outer membrane Source: RGD
  • membrane raft Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: RGD
  • ruffle membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi388D → N: Abolishes phosphatase activity. 1 Publication1
Mutagenesisi435G → V: Reduced phosphatase activity. 1 Publication1
Mutagenesisi466R → H: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002097351 – 1496Synaptojanin-2Add BLAST1496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1139PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO55207.
PRIDEiO55207.

PTM databases

iPTMnetiO55207.
PhosphoSitePlusiO55207.

Expressioni

Tissue specificityi

Widely expressed. Isoforms 2B1 and 2B2 are concentrated at nerve terminals in brain and at spermatid manchette in testis.

Interactioni

Subunit structurei

Binds to GRB2 (By similarity). Isoform 2A binds to SYNJ2BP/OMP25.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Synj2bpQ9WVJ49EBI-7007476,EBI-7007454

GO - Molecular functioni

  • PDZ domain binding Source: RGD
  • SH3 domain binding Source: RGD

Protein-protein interaction databases

IntActiO55207. 1 interactor.
MINTiMINT-132533.
STRINGi10116.ENSRNOP00000062564.

Structurei

3D structure databases

ProteinModelPortaliO55207.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini120 – 444SACPROSITE-ProRule annotationAdd BLAST325
Domaini906 – 985RRMPROSITE-ProRule annotationAdd BLAST80

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni450 – ?CatalyticBy similarity

Sequence similaritiesi

Belongs to the synaptojanin family.Curated
In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation
Contains 1 SAC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0566. Eukaryota.
COG5329. LUCA.
COG5411. LUCA.
HOGENOMiHOG000007937.
HOVERGENiHBG079225.
InParanoidiO55207.
KOiK20279.
PhylomeDBiO55207.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
3.60.10.10. 1 hit.
InterProiIPR015047. DUF1866.
IPR005135. Endo/exonuclease/phosphatase.
IPR000300. IPPc.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR002013. SAC_dom.
[Graphical view]
PfamiPF08952. DUF1866. 1 hit.
PF03372. Exo_endo_phos. 1 hit.
PF02383. Syja_N. 1 hit.
[Graphical view]
SMARTiSM01165. DUF1866. 1 hit.
SM00128. IPPc. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
SSF56219. SSF56219. 2 hits.
PROSITEiPS50102. RRM. 1 hit.
PS50275. SAC. 1 hit.
[Graphical view]

Sequences (7)i

Sequence statusi: Complete.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms.
Isoform 2B2 (identifier: O55207-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALSKGLRLL ARLDPTGPSS VLLEARGRGD CLLFEAGAVA TLAPEEKEVI
60 70 80 90 100
KGLYGKPTDA YGCLGELSLK SGGVPLSFLV LVTGCTSVGR IPDAEIYKIT
110 120 130 140 150
GTEFYPLQEE AKEEDRLPAL KKILSSGVFY FAWPNDGACF DLTIRAQKQG
160 170 180 190 200
DDCSEWGTSF FWNQLLHVPL RQHQVNCHDW LLKVICGVVT IRTVYASHKQ
210 220 230 240 250
AKACLISRIS CERAGARFLT RGVNDDGHVS NFVETEQAIY MDDGVSSFVQ
260 270 280 290 300
IRGSVPLFWE QPGLQVGSHH LRLHRGLEAN APAFERHMVL LKEQYGQQVV
310 320 330 340 350
VNLLGSRGGE EVLNRAFKKL LWASCHAGDT PMINFDFHQF AKGRKLEKLE
360 370 380 390 400
NLLRPQLKLH WDDFGVFAKG ENVSPRFQKG TLRMNCLDCL DRTNTVQCFI
410 420 430 440 450
ALEVLHLQLE SLGLNSKPIT DRFVESFKAM WSLNGHGLSK VFTGSRALEG
460 470 480 490 500
KAKVGKLKDG ARSMSRTIQS NFFDGVKQEA IKLLLVGDVY NEESTDKGRM
510 520 530 540 550
LLDNTALLGL GSNKQNSLSG MLDGKATPRI LKAMTERQSE FTNFKRIQIA
560 570 580 590 600
MGTWNVNGGK QFRSNLLGTT ELTDWLLDAP QLSGAVDSQD DGGPADIFAV
610 620 630 640 650
GFEEMVELSA GNIVNASTTN RKMWGEQLQK AISRSHRYIL LTSAQLVGVC
660 670 680 690 700
LYIFVRPYHV PFIRDVAIDT VKTGMGGKAG NKGAVGIRFQ FHSTSFCFIC
710 720 730 740 750
SHLTAGQSQV KERNEDYREI THKLSFPSGR NIFSHDYVFW CGDFNYRIDL
760 770 780 790 800
TYEEVFYFVK RQDWKKLMEF DQLQLQKSSG KIFKDFHEGT INFGPTYKYD
810 820 830 840 850
VGSAAYDTSD KCRTPAWTDR VLWWRKKHPY DKTAGELNLL DSDLDGDANI
860 870 880 890 900
RHTWSPGTLK YYGRAELQAS DHRPVLAIVE VEVQEVDVGA RERVFQEVSS
910 920 930 940 950
VQGPLDATVI VNLQSPTLEE RNEFPEDLRT ELMQTLGNYG TIILVRINQG
960 970 980 990 1000
QMLVTFADSH SALSVLDVDG MKVKGRAVKI RPKTKDWLEG LREELIRKRD
1010 1020 1030 1040 1050
SMAPVSPTAN SCLLEENFDF TSLDYESEGD VLEDDEDYLA DEFGQPVVSD
1060 1070 1080 1090 1100
SELGGDDSSD TMSASTPASK SPALAKKKQH PTYKDDADLM TLKLELEVAG
1110 1120 1130 1140 1150
NFRHRSPSRS LSVPNRPRPP HPPQRPPPPT GLMVKKSASD ASISSGTHGQ
1160 1170 1180 1190 1200
YSILQTAKLL PGAPQQPPKA RTGISKPYNV KQIKTTNAQE AEAAIRCLLE
1210 1220 1230 1240 1250
AGGGVPESAP GATPLRNQGS SKPEASLGPP VLPRRPVPRV PTMKKPTLRR
1260 1270 1280 1290 1300
TGKPMLPEEQ CEQQPVHFTM ASQEMNLETP PPITAPIPPV PKPRTFQPGR
1310 1320 1330 1340 1350
GVERRPSGGK PEPDDAPPVT GAVELSSPEA PEAPSLAPKV PPRRKKSAPA
1360 1370 1380 1390 1400
AFHLQVLQSN SQLLQGLTCS SSSPSPPKPD TPLLYPQMAL GTSSAISPET
1410 1420 1430 1440 1450
DGPRVTEPEA ASFHGDYPDP FWSLLHHPKL LNNNTWLSKS SEPLDLGSRT
1460 1470 1480 1490
PERTHTDSAQ VNASVVERGL PPDHGGKDFS HWMAASNKDK RTTLGV
Length:1,496
Mass (Da):165,264
Last modified:March 1, 2005 - v2
Checksum:iCB15017BF7821648
GO
Isoform 2B1 (identifier: O55207-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1085-1129: Missing.

Show »
Length:1,451
Mass (Da):160,205
Checksum:i055F3EC87DE70109
GO
Isoform 2A (identifier: O55207-3) [UniParc]FASTAAdd to basket
Also known as: 7.5kb

The sequence of this isoform differs from the canonical sequence as follows:
     1254-1293: PMLPEEQCEQ...TAPIPPVPKP → IVFCSSSQAS...TPVDASGSSV
     1294-1496: Missing.

Note: The PDZ domain of isoform 2A binds SYNJ2BP/OMP25. Mutagenesis of Ser-1246 or Val-1248 to Ala abolishes SYNJ2BP/OMP25 binding.
Show »
Length:1,293
Mass (Da):143,364
Checksum:i0DE5F7FAC1425ACE
GO
Isoform 7.2kb (identifier: O55207-4)
Sequence is not available
Length:
Mass (Da):
Isoform 6.0kb (identifier: O55207-5)
Sequence is not available
Length:
Mass (Da):
Isoform 5.2kb (identifier: O55207-6)
Sequence is not available
Length:
Mass (Da):
Isoform 3.5kb (identifier: O55207-7)
Sequence is not available
Length:
Mass (Da):

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1130T → A in AAK61722 (PubMed:11498538).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0129141085 – 1129Missing in isoform 2B1. 1 PublicationAdd BLAST45
Alternative sequenceiVSP_0129151254 – 1293PMLPE…PVPKP → IVFCSSSQASQPCSLLQRHE FVRTVAAQRLTPVDASGSSV in isoform 2A. 1 PublicationAdd BLAST40
Alternative sequenceiVSP_0129161294 – 1496Missing in isoform 2A. 1 PublicationAdd BLAST203

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90312 mRNA. Translation: AAB92481.1.
AY034050 mRNA. Translation: AAK61722.1.
AY034051 mRNA. Translation: AAK61723.1.
RefSeqiNP_001106842.1. NM_001113371.1.
NP_001106843.1. NM_001113372.1.
NP_114460.1. NM_032071.2.
UniGeneiRn.10868.

Genome annotation databases

GeneIDi84018.
KEGGirno:84018.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90312 mRNA. Translation: AAB92481.1.
AY034050 mRNA. Translation: AAK61722.1.
AY034051 mRNA. Translation: AAK61723.1.
RefSeqiNP_001106842.1. NM_001113371.1.
NP_001106843.1. NM_001113372.1.
NP_114460.1. NM_032071.2.
UniGeneiRn.10868.

3D structure databases

ProteinModelPortaliO55207.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO55207. 1 interactor.
MINTiMINT-132533.
STRINGi10116.ENSRNOP00000062564.

PTM databases

iPTMnetiO55207.
PhosphoSitePlusiO55207.

Proteomic databases

PaxDbiO55207.
PRIDEiO55207.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84018.
KEGGirno:84018.

Organism-specific databases

CTDi8871.
RGDi69436. Synj2.

Phylogenomic databases

eggNOGiKOG0566. Eukaryota.
COG5329. LUCA.
COG5411. LUCA.
HOGENOMiHOG000007937.
HOVERGENiHBG079225.
InParanoidiO55207.
KOiK20279.
PhylomeDBiO55207.

Miscellaneous databases

PROiO55207.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
3.60.10.10. 1 hit.
InterProiIPR015047. DUF1866.
IPR005135. Endo/exonuclease/phosphatase.
IPR000300. IPPc.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR002013. SAC_dom.
[Graphical view]
PfamiPF08952. DUF1866. 1 hit.
PF03372. Exo_endo_phos. 1 hit.
PF02383. Syja_N. 1 hit.
[Graphical view]
SMARTiSM01165. DUF1866. 1 hit.
SM00128. IPPc. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
SSF56219. SSF56219. 2 hits.
PROSITEiPS50102. RRM. 1 hit.
PS50275. SAC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYNJ2_RAT
AccessioniPrimary (citable) accession number: O55207
Secondary accession number(s): Q91ZD8, Q91ZD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: March 1, 2005
Last modified: November 30, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.