Reviewed, UniProtKB/Swiss-Prot O55207 (SYNJ2_RAT)

Names and origin

Protein names

Recommended name:
    Synaptojanin-2
    EC=3.1.3.36
Alternative name(s):
    Synaptic inositol-1,4,5-trisphosphate 5-phosphatase 2

Gene names

Name:

Synj2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1496 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inositol 5-phosphatase which may be involved in distinct membrane trafficking and signal transduction pathways. May mediate the inhibitory effect of Rac1 on endocytosis By similarity.
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.
Subunit structure	Binds to GRB2 By similarity. Isoform 2A binds to SYNJ2BP/OMP25.
Subcellular location	Cytoplasm. Cell membrane. Note: Rac1 causes translocation from cytosol to the plasma membrane. Ref.2 Isoform 2A: Mitochondrion. Note: Interaction of isoform 2A with SYNJ2BP/OMP25 results in localization to the mitochondrion. Ref.2
Tissue specificity	Widely expressed. Isoforms 2B1 and 2B2 are concentrated at nerve terminals in brain and at spermatid manchette in testis.
Sequence similarities	Belongs to the synaptojanin family. In the central section; belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family. Contains 1 RRM (RNA recognition motif) domain. Contains 1 SAC domain.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane Mitochondrion
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	brain development Inferred from expression pattern. Source: RGD inositol phosphate dephosphorylation Ref.1 Inferred from direct assay. Source: RGD intracellular distribution of mitochondria Ref.3 Inferred from direct assay. Source: RGD phosphoinositide dephosphorylation Ref.2 Inferred from mutant phenotype. Source: RGD
Cellular component	cytoplasmic microtubule Ref.2 Inferred from direct assay. Source: RGD extrinsic to mitochondrial outer membrane Ref.3 Inferred from direct assay. Source: RGD insoluble fraction Ref.1 Inferred from direct assay. Source: RGD nerve terminal Ref.2 Inferred from direct assay. Source: RGD perinuclear region of cytoplasm Ref.3 Inferred from direct assay. Source: RGD ruffle membrane Ref.2 Inferred from direct assay. Source: RGD synapse Ref.1 Traceable author statement. Source: RGD
Molecular function	PDZ domain binding Ref.2 Ref.3 Inferred from physical interaction. Source: RGD RNA binding Inferred from electronic annotation. Source: UniProtKB-KW SH3 domain binding Ref.1 Ref.2 Inferred from physical interaction. Source: RGD inositol-polyphosphate 5-phosphatase activity Ref.1 Inferred from direct assay. Source: RGD phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms.

Isoform 2B2 (identifier: O55207-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2B1 (identifier: O55207-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1085-1129: Missing.

Isoform 2A (identifier: O55207-3)

Also known as: 7.5kb;

The sequence of this isoform differs from the canonical sequence as follows:
     1254-1293: PMLPEEQCEQ...TAPIPPVPKP → IVFCSSSQAS...TPVDASGSSV
     1294-1496: Missing.

Note: Interaction of isoform 2A with SYNJ2BP/OMP25 results in localization to the mitochondrion. Ref.2 The PDZ domain of isoform 2A binds SYNJ2BP/OMP25. Mutagenesis of Ser-1246 or Val-1248 to Ala abolishes SYNJ2BP/OMP25 binding.

Isoform 7.2kb (identifier: O55207-4)

The sequence of this isoform is not available.

Isoform 6.0kb (identifier: O55207-5)

The sequence of this isoform is not available.

Isoform 5.2kb (identifier: O55207-6)

The sequence of this isoform is not available.

Isoform 3.5kb (identifier: O55207-7)

The sequence of this isoform is not available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1496	1496	Synaptojanin-2		PRO_0000209735
Regions
	Domain	120 – 444	325	SAC
	Domain	906 – 985	80	RRM
	Region	450 – ?		Catalytic By similarity
Amino acid modifications
	Modified residue	130	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	1085 – 1129	45	Missing in isoform 2B1.		VSP_012914
	Alternative sequence	1254 – 1293	40	PMLPE…PVPKP → IVFCSSSQASQPCSLLQRHE FVRTVAAQRLTPVDASGSSV in isoform 2A.		VSP_012915
	Alternative sequence	1294 – 1496	203	Missing in isoform 2A.		VSP_012916
Experimental info
	Mutagenesis	388	1	D → N: Abolishes phosphatase activity. Ref.2
	Mutagenesis	435	1	G → V: Reduced phosphatase activity. Ref.2
	Mutagenesis	466	1	R → H: No effect. Ref.2
	Sequence conflict	1130	1	T → A in AAK61722. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2B2 [UniParc]. Last modified March 1, 2005. Version 2. Checksum: CB15017BF7821648	FASTA	1,496	165,264
	10 20 30 40 50 60 MALSKGLRLL ARLDPTGPSS VLLEARGRGD CLLFEAGAVA TLAPEEKEVI KGLYGKPTDA 70 80 90 100 110 120 YGCLGELSLK SGGVPLSFLV LVTGCTSVGR IPDAEIYKIT GTEFYPLQEE AKEEDRLPAL 130 140 150 160 170 180 KKILSSGVFY FAWPNDGACF DLTIRAQKQG DDCSEWGTSF FWNQLLHVPL RQHQVNCHDW 190 200 210 220 230 240 LLKVICGVVT IRTVYASHKQ AKACLISRIS CERAGARFLT RGVNDDGHVS NFVETEQAIY 250 260 270 280 290 300 MDDGVSSFVQ IRGSVPLFWE QPGLQVGSHH LRLHRGLEAN APAFERHMVL LKEQYGQQVV 310 320 330 340 350 360 VNLLGSRGGE EVLNRAFKKL LWASCHAGDT PMINFDFHQF AKGRKLEKLE NLLRPQLKLH 370 380 390 400 410 420 WDDFGVFAKG ENVSPRFQKG TLRMNCLDCL DRTNTVQCFI ALEVLHLQLE SLGLNSKPIT 430 440 450 460 470 480 DRFVESFKAM WSLNGHGLSK VFTGSRALEG KAKVGKLKDG ARSMSRTIQS NFFDGVKQEA 490 500 510 520 530 540 IKLLLVGDVY NEESTDKGRM LLDNTALLGL GSNKQNSLSG MLDGKATPRI LKAMTERQSE 550 560 570 580 590 600 FTNFKRIQIA MGTWNVNGGK QFRSNLLGTT ELTDWLLDAP QLSGAVDSQD DGGPADIFAV 610 620 630 640 650 660 GFEEMVELSA GNIVNASTTN RKMWGEQLQK AISRSHRYIL LTSAQLVGVC LYIFVRPYHV 670 680 690 700 710 720 PFIRDVAIDT VKTGMGGKAG NKGAVGIRFQ FHSTSFCFIC SHLTAGQSQV KERNEDYREI 730 740 750 760 770 780 THKLSFPSGR NIFSHDYVFW CGDFNYRIDL TYEEVFYFVK RQDWKKLMEF DQLQLQKSSG 790 800 810 820 830 840 KIFKDFHEGT INFGPTYKYD VGSAAYDTSD KCRTPAWTDR VLWWRKKHPY DKTAGELNLL 850 860 870 880 890 900 DSDLDGDANI RHTWSPGTLK YYGRAELQAS DHRPVLAIVE VEVQEVDVGA RERVFQEVSS 910 920 930 940 950 960 VQGPLDATVI VNLQSPTLEE RNEFPEDLRT ELMQTLGNYG TIILVRINQG QMLVTFADSH 970 980 990 1000 1010 1020 SALSVLDVDG MKVKGRAVKI RPKTKDWLEG LREELIRKRD SMAPVSPTAN SCLLEENFDF 1030 1040 1050 1060 1070 1080 TSLDYESEGD VLEDDEDYLA DEFGQPVVSD SELGGDDSSD TMSASTPASK SPALAKKKQH 1090 1100 1110 1120 1130 1140 PTYKDDADLM TLKLELEVAG NFRHRSPSRS LSVPNRPRPP HPPQRPPPPT GLMVKKSASD 1150 1160 1170 1180 1190 1200 ASISSGTHGQ YSILQTAKLL PGAPQQPPKA RTGISKPYNV KQIKTTNAQE AEAAIRCLLE 1210 1220 1230 1240 1250 1260 AGGGVPESAP GATPLRNQGS SKPEASLGPP VLPRRPVPRV PTMKKPTLRR TGKPMLPEEQ 1270 1280 1290 1300 1310 1320 CEQQPVHFTM ASQEMNLETP PPITAPIPPV PKPRTFQPGR GVERRPSGGK PEPDDAPPVT 1330 1340 1350 1360 1370 1380 GAVELSSPEA PEAPSLAPKV PPRRKKSAPA AFHLQVLQSN SQLLQGLTCS SSSPSPPKPD 1390 1400 1410 1420 1430 1440 TPLLYPQMAL GTSSAISPET DGPRVTEPEA ASFHGDYPDP FWSLLHHPKL LNNNTWLSKS 1450 1460 1470 1480 1490 SEPLDLGSRT PERTHTDSAQ VNASVVERGL PPDHGGKDFS HWMAASNKDK RTTLGV
	Isoform 2B1. Checksum: 055F3EC87DE70109	FASTA	1,451	160,205
	10 20 30 40 50 60 MALSKGLRLL ARLDPTGPSS VLLEARGRGD CLLFEAGAVA TLAPEEKEVI KGLYGKPTDA 70 80 90 100 110 120 YGCLGELSLK SGGVPLSFLV LVTGCTSVGR IPDAEIYKIT GTEFYPLQEE AKEEDRLPAL 130 140 150 160 170 180 KKILSSGVFY FAWPNDGACF DLTIRAQKQG DDCSEWGTSF FWNQLLHVPL RQHQVNCHDW 190 200 210 220 230 240 LLKVICGVVT IRTVYASHKQ AKACLISRIS CERAGARFLT RGVNDDGHVS NFVETEQAIY 250 260 270 280 290 300 MDDGVSSFVQ IRGSVPLFWE QPGLQVGSHH LRLHRGLEAN APAFERHMVL LKEQYGQQVV 310 320 330 340 350 360 VNLLGSRGGE EVLNRAFKKL LWASCHAGDT PMINFDFHQF AKGRKLEKLE NLLRPQLKLH 370 380 390 400 410 420 WDDFGVFAKG ENVSPRFQKG TLRMNCLDCL DRTNTVQCFI ALEVLHLQLE SLGLNSKPIT 430 440 450 460 470 480 DRFVESFKAM WSLNGHGLSK VFTGSRALEG KAKVGKLKDG ARSMSRTIQS NFFDGVKQEA 490 500 510 520 530 540 IKLLLVGDVY NEESTDKGRM LLDNTALLGL GSNKQNSLSG MLDGKATPRI LKAMTERQSE 550 560 570 580 590 600 FTNFKRIQIA MGTWNVNGGK QFRSNLLGTT ELTDWLLDAP QLSGAVDSQD DGGPADIFAV 610 620 630 640 650 660 GFEEMVELSA GNIVNASTTN RKMWGEQLQK AISRSHRYIL LTSAQLVGVC LYIFVRPYHV 670 680 690 700 710 720 PFIRDVAIDT VKTGMGGKAG NKGAVGIRFQ FHSTSFCFIC SHLTAGQSQV KERNEDYREI 730 740 750 760 770 780 THKLSFPSGR NIFSHDYVFW CGDFNYRIDL TYEEVFYFVK RQDWKKLMEF DQLQLQKSSG 790 800 810 820 830 840 KIFKDFHEGT INFGPTYKYD VGSAAYDTSD KCRTPAWTDR VLWWRKKHPY DKTAGELNLL 850 860 870 880 890 900 DSDLDGDANI RHTWSPGTLK YYGRAELQAS DHRPVLAIVE VEVQEVDVGA RERVFQEVSS 910 920 930 940 950 960 VQGPLDATVI VNLQSPTLEE RNEFPEDLRT ELMQTLGNYG TIILVRINQG QMLVTFADSH 970 980 990 1000 1010 1020 SALSVLDVDG MKVKGRAVKI RPKTKDWLEG LREELIRKRD SMAPVSPTAN SCLLEENFDF 1030 1040 1050 1060 1070 1080 TSLDYESEGD VLEDDEDYLA DEFGQPVVSD SELGGDDSSD TMSASTPASK SPALAKKKQH 1090 1100 1110 1120 1130 1140 PTYKTGLMVK KSASDASISS GTHGQYSILQ TAKLLPGAPQ QPPKARTGIS KPYNVKQIKT 1150 1160 1170 1180 1190 1200 TNAQEAEAAI RCLLEAGGGV PESAPGATPL RNQGSSKPEA SLGPPVLPRR PVPRVPTMKK 1210 1220 1230 1240 1250 1260 PTLRRTGKPM LPEEQCEQQP VHFTMASQEM NLETPPPITA PIPPVPKPRT FQPGRGVERR 1270 1280 1290 1300 1310 1320 PSGGKPEPDD APPVTGAVEL SSPEAPEAPS LAPKVPPRRK KSAPAAFHLQ VLQSNSQLLQ 1330 1340 1350 1360 1370 1380 GLTCSSSSPS PPKPDTPLLY PQMALGTSSA ISPETDGPRV TEPEAASFHG DYPDPFWSLL 1390 1400 1410 1420 1430 1440 HHPKLLNNNT WLSKSSEPLD LGSRTPERTH TDSAQVNASV VERGLPPDHG GKDFSHWMAA 1450 SNKDKRTTLG V
	Isoform 2A (7.5kb). Checksum: 0DE5F7FAC1425ACE	FASTA	1,293	143,364
	10 20 30 40 50 60 MALSKGLRLL ARLDPTGPSS VLLEARGRGD CLLFEAGAVA TLAPEEKEVI KGLYGKPTDA 70 80 90 100 110 120 YGCLGELSLK SGGVPLSFLV LVTGCTSVGR IPDAEIYKIT GTEFYPLQEE AKEEDRLPAL 130 140 150 160 170 180 KKILSSGVFY FAWPNDGACF DLTIRAQKQG DDCSEWGTSF FWNQLLHVPL RQHQVNCHDW 190 200 210 220 230 240 LLKVICGVVT IRTVYASHKQ AKACLISRIS CERAGARFLT RGVNDDGHVS NFVETEQAIY 250 260 270 280 290 300 MDDGVSSFVQ IRGSVPLFWE QPGLQVGSHH LRLHRGLEAN APAFERHMVL LKEQYGQQVV 310 320 330 340 350 360 VNLLGSRGGE EVLNRAFKKL LWASCHAGDT PMINFDFHQF AKGRKLEKLE NLLRPQLKLH 370 380 390 400 410 420 WDDFGVFAKG ENVSPRFQKG TLRMNCLDCL DRTNTVQCFI ALEVLHLQLE SLGLNSKPIT 430 440 450 460 470 480 DRFVESFKAM WSLNGHGLSK VFTGSRALEG KAKVGKLKDG ARSMSRTIQS NFFDGVKQEA 490 500 510 520 530 540 IKLLLVGDVY NEESTDKGRM LLDNTALLGL GSNKQNSLSG MLDGKATPRI LKAMTERQSE 550 560 570 580 590 600 FTNFKRIQIA MGTWNVNGGK QFRSNLLGTT ELTDWLLDAP QLSGAVDSQD DGGPADIFAV 610 620 630 640 650 660 GFEEMVELSA GNIVNASTTN RKMWGEQLQK AISRSHRYIL LTSAQLVGVC LYIFVRPYHV 670 680 690 700 710 720 PFIRDVAIDT VKTGMGGKAG NKGAVGIRFQ FHSTSFCFIC SHLTAGQSQV KERNEDYREI 730 740 750 760 770 780 THKLSFPSGR NIFSHDYVFW CGDFNYRIDL TYEEVFYFVK RQDWKKLMEF DQLQLQKSSG 790 800 810 820 830 840 KIFKDFHEGT INFGPTYKYD VGSAAYDTSD KCRTPAWTDR VLWWRKKHPY DKTAGELNLL 850 860 870 880 890 900 DSDLDGDANI RHTWSPGTLK YYGRAELQAS DHRPVLAIVE VEVQEVDVGA RERVFQEVSS 910 920 930 940 950 960 VQGPLDATVI VNLQSPTLEE RNEFPEDLRT ELMQTLGNYG TIILVRINQG QMLVTFADSH 970 980 990 1000 1010 1020 SALSVLDVDG MKVKGRAVKI RPKTKDWLEG LREELIRKRD SMAPVSPTAN SCLLEENFDF 1030 1040 1050 1060 1070 1080 TSLDYESEGD VLEDDEDYLA DEFGQPVVSD SELGGDDSSD TMSASTPASK SPALAKKKQH 1090 1100 1110 1120 1130 1140 PTYKDDADLM TLKLELEVAG NFRHRSPSRS LSVPNRPRPP HPPQRPPPPT GLMVKKSASD 1150 1160 1170 1180 1190 1200 ASISSGTHGQ YSILQTAKLL PGAPQQPPKA RTGISKPYNV KQIKTTNAQE AEAAIRCLLE 1210 1220 1230 1240 1250 1260 AGGGVPESAP GATPLRNQGS SKPEASLGPP VLPRRPVPRV PTMKKPTLRR TGKIVFCSSS 1270 1280 1290 QASQPCSLLQ RHEFVRTVAA QRLTPVDASG SSV
	Isoform 7.2kb (Sequence not available).	FASTA	–	–
	Isoform 6.0kb (Sequence not available).	FASTA	–	–
	Isoform 5.2kb (Sequence not available).	FASTA	–	–
	Isoform 3.5kb (Sequence not available).	FASTA	–	–

References

[1]	"Synaptojanin 2, a novel synaptojanin isoform with a distinct targeting domain and expression pattern." Nemoto Y., Arribas M., Haffner C., de Camilli P. J. Biol. Chem. 272:30817-30821(1997) [PubMed: 9388224] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2A), CHARACTERIZATION. Tissue: Brain.
[2]	"Identification and characterization of a synaptojanin 2 splice isoform predominantly expressed in nerve terminals." Nemoto Y., Wenk M.R., Watanabe M., Daniell L., Murakami T., Ringstad N., Yamada H., Takei K., De Camilli P. J. Biol. Chem. 276:41133-41142(2001) [PubMed: 11498538] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2B1 AND 2B2), SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-388; GLY-435 AND ARG-466. Strain: Sprague-Dawley.
[3]	"Recruitment of an alternatively spliced form of synaptojanin 2 to mitochondria by the interaction with the PDZ domain of a mitochondrial outer membrane protein." Nemoto Y., De Camilli P. EMBO J. 18:2991-3006(1999) [PubMed: 10357812] [Abstract] Cited for: INTERACTION WITH SYNJ2BP, MUTAGENESIS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U90312 mRNA. Translation: AAB92481.1. AY034050 mRNA. Translation: AAK61722.1. AY034051 mRNA. Translation: AAK61723.1.
IPI	IPI00196724. IPI00400483. IPI00515859.
RefSeq	NP_001106842.1. NP_001106843.1.
UniGene	Rn.10868
3D structure databases
HSSP	HSSP built from PDB template 1I9Z based on UniProtKB O43001.
SMR	O55207. Positions 902-980.
ModBase	Search...
Protein-protein interaction databases
STRING	O55207.
PTM databases
PhosphoSite	O55207.
Genome annotation databases
GeneID	84018.
KEGG	rno:84018.
Organism-specific databases
CTD	84018.
RGD	69436. Synj2.
Phylogenomic databases
HOVERGEN	O55207.
Enzyme and pathway databases
BRENDA	3.1.3.36. 248.
Gene expression databases
Genevestigator	O55207.
Family and domain databases
InterPro	IPR015047. DUF1866. IPR005135. Endo/exonuclease/phosphatase. IPR000300. IPPc. IPR000504. RRM_RNP1. IPR002013. Syja_N. [Graphical view]
Pfam	PF08952. DUF1866. 1 hit. PF03372. Exo_endo_phos. 1 hit. PF02383. Syja_N. 1 hit. [Graphical view]
SMART	SM00128. IPPc. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. PS50275. SAC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	616569.

Entry information

Entry name

SYNJ2_RAT

Accession

Primary (citable) accession number: O55207
Secondary accession number(s): Q91ZD8, Q91ZD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	March 1, 2005
Last modified:	October 13, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)