O55193 (CCR2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-C chemokine receptor type 2
Short name=C-C CKR-2
Short name=CC-CKR-2
Short name=CCR-2
Short name=CCR2

Alternative name(s):
CD_antigen=CD192

Gene names

Name:	Ccr2
Synonyms:	Cmkbr2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for the MCP-1 (JE), MCP-3 (FIC) and MCP-5 chemokines. Transduces a signal by increasing the intracellular calcium ions level By similarity.
Subunit structure	Interacts with ARRB1 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in lung, spleen, kidney, thymus and macrophages.
Induction	In animals in which experimental allergic encephalomyelitis (EAE) has been induced.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T-helper 17 cell chemotaxis Inferred from sequence or structural similarity. Source: BHF-UCL blood vessel remodeling Inferred from expression pattern PubMed 15178559. Source: RGD cellular calcium ion homeostasis Inferred from direct assay PubMed 15978006. Source: RGD elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: InterPro inflammatory response to antigenic stimulus Inferred from expression pattern PubMed 14674010. Source: RGD negative regulation of adenylate cyclase activity Inferred from electronic annotation. Source: InterPro negative regulation of angiogenesis Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of eosinophil degranulation Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of type 2 immune response Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of CD8-positive, alpha-beta T cell extravasation Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of MAPK cascade Inferred from direct assay PubMed 18584881. Source: RGD positive regulation of T cell chemotaxis Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of T-helper 1 type immune response Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of alpha-beta T cell proliferation Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of astrocyte chemotaxis Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of hematopoietic stem cell migration Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of immune complex clearance by monocytes and macrophages Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of inflammatory response Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of interferon-gamma production Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of interleukin-2 production Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of monocyte chemotaxis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of monocyte extravasation Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of tumor necrosis factor biosynthetic process Inferred from sequence or structural similarity. Source: BHF-UCL regulation of vascular endothelial growth factor production Inferred from sequence or structural similarity. Source: BHF-UCL response to hypoxia Inferred from expression pattern PubMed 18446360. Source: RGD
Cellular_component	cytosol Inferred from direct assay PubMed 15978006. Source: RGD dendrite Inferred from direct assay PubMed 15978006PubMed 16196033PubMed 18584881. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW perikaryon Inferred from direct assay PubMed 16196033. Source: RGD perinuclear region of cytoplasm Inferred from direct assay PubMed 15978006. Source: RGD plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	C-C chemokine binding Inferred from mutant phenotype PubMed 16196033. Source: RGD C-C chemokine receptor activity Inferred from mutant phenotype PubMed 16196033. Source: RGD protein homodimerization activity Inferred from direct assay PubMed 15978006. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	C-C chemokine receptor type 2		PRO_0000069235
Regions
	Topological domain	1 – 60	60	Extracellular Potential
	Transmembrane	61 – 81	21	Helical; Name=1; Potential
	Topological domain	82 – 91	10	Cytoplasmic Potential
	Transmembrane	92 – 112	21	Helical; Name=2; Potential
	Topological domain	113 – 128	16	Extracellular Potential
	Transmembrane	129 – 149	21	Helical; Name=3; Potential
	Topological domain	150 – 170	21	Cytoplasmic Potential
	Transmembrane	171 – 191	21	Helical; Name=4; Potential
	Topological domain	192 – 220	29	Extracellular Potential
	Transmembrane	221 – 241	21	Helical; Name=5; Potential
	Topological domain	242 – 256	15	Cytoplasmic Potential
	Transmembrane	257 – 277	21	Helical; Name=6; Potential
	Topological domain	278 – 301	24	Extracellular Potential
	Transmembrane	302 – 322	21	Helical; Name=7; Potential
	Topological domain	323 – 373	51	Cytoplasmic Potential
Amino acid modifications
	Modified residue	152	1	Phosphotyrosine; by JAK2 By similarity
	Disulfide bond	45 ↔ 290		By similarity
	Disulfide bond	126 ↔ 203		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O55193 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 2E7BB012F5D6FD09	FASTA	373	42,763
	10 20 30 40 50 60 MEDSNMLPQF IHGILSTSHS LFPRSIQELD EGATTPYDYD DGEPCHKTSV KQIGAWILPP 70 80 90 100 110 120 LYSLVFIFGF VGNMLVIIIL ISCKKLKSMT DIYLFNLAIS DLLFLLTLPF WAHYAANEWV 130 140 150 160 170 180 FGNIMCKLFT GLYHIGYFGG IFFIILLTID RYLAIVHAVF ALKARTVTFG VITSVVTWVV 190 200 210 220 230 240 AVFASLPGII FTKSEQEDDQ HTCGPYFPTI WKNFQTIMRN ILSLILPLLV MVICYSGILH 250 260 270 280 290 300 TLFRCRNEKK RHRAVRLIFA IMIVYFLFWT PYNIVLFLTT FQEFLGMSNC VVDMHLDQAM 310 320 330 340 350 360 QVTETLGMTH CCVNPIIYAF VGEKFRRYLS IFFRKHIAKN LCKQCPVFYR ETADRVSSTF 370 TPSTGEQEVS VGL

References

[1]	"Chemokine receptor expression in cultured glia and rat experimental allergic encephalomyelitis." Jiang Y., Salafranca M.N., Adhikari S., Xia Y., Feng L., Sonntag M.K., deFiebre C.M., Pennell N.A., Streit W.J., Harrison J.K. J. Neuroimmunol. 86:1-12(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U77349 Genomic DNA. Translation: AAC03242.1.
IPI	IPI00196054.
RefSeq	NP_068638.1. NM_021866.1.
UniGene	Rn.211983.
3D structure databases
ProteinModelPortal	O55193.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000008843.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	O55193.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	60463.
KEGG	rno:60463.
UCSC	RGD:620876. rat.
Organism-specific databases
CTD	729230.
RGD	620876. Ccr2.
Phylogenomic databases
eggNOG	NOG148353.
HOGENOM	HOG000234122.
HOVERGEN	HBG106917.
InParanoid	O55193.
KO	K04177.
OMA	CPVFYRE.
OrthoDB	EOG40S0GG.
Gene expression databases
Genevestigator	O55193.
GermOnline	ENSRNOG00000006755. Rattus norvegicus.
Family and domain databases
InterPro	IPR002237. Chemokine_CCR2. IPR000355. Chemokine_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00657. CCCHEMOKINER. PR01107. CHEMOKINER2. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	O55193.
ChEMBL	CHEMBL1293204.
NextBio	612222.

Entry information

Entry name

CCR2_RAT

Accession

Primary (citable) accession number: O55193

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program