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Protein

E3 ubiquitin-protein ligase Praja-1

Gene

Pja1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome. May be involved in protein sorting.2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri530 – 571RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: MGI

GO - Biological processi

  • protein catabolic process Source: MGI

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Praja-1 (EC:2.3.2.27)
Short name:
Praja1
Alternative name(s):
RING-type E3 ubiquitin transferase Praja-1Curated
Gene namesi
Name:Pja1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1101765. Pja1.

Subcellular locationi

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi533C → A: No effect on MAGED1 binding. Decrease in ubiquitination of MAGED1. No inhibition of DLX5-dependent transcriptional activity. 1 Publication1
Mutagenesisi553H → S: Loss of ubiquitination activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000560001 – 578E3 ubiquitin-protein ligase Praja-1Add BLAST578

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei231PhosphothreonineCombined sources1
Modified residuei317PhosphoserineCombined sources1
Modified residuei319PhosphoserineCombined sources1

Post-translational modificationi

Substrate for E2-dependent ubiquitination.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO55176.
PeptideAtlasiO55176.
PRIDEiO55176.

PTM databases

iPTMnetiO55176.
PhosphoSitePlusiO55176.

Expressioni

Tissue specificityi

Expressed in brain, liver, kidney. Highest levels in brain where it is found in many regions including cortical and subcortical areas and in neurons of the amygdala. Weak expression also found in testis. Also expressed in developing embryo.

Inductioni

By fear memory. Differential induction of isoforms.

Gene expression databases

BgeeiENSMUSG00000034403.
CleanExiMM_PJA1.
ExpressionAtlasiO55176. baseline and differential.
GenevisibleiO55176. MM.

Interactioni

Subunit structurei

Binds ubiquitin-conjugating enzymes (E2s). Binds, in vitro and in vivo, the MAGE conserved domain of MAGED1. Binds weakly Necdin, in vitro. Interacts with UBE2D2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Maged1Q9QYH62EBI-1801670,EBI-1801274

Protein-protein interaction databases

BioGridi202189. 2 interactors.
IntActiO55176. 3 interactors.
STRINGi10090.ENSMUSP00000109420.

Structurei

3D structure databases

ProteinModelPortaliO55176.
SMRiO55176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi230 – 236Poly-Asp7
Compositional biasi292 – 295Poly-Arg4

Domaini

The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri530 – 571RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0800. Eukaryota.
ENOG41121N2. LUCA.
GeneTreeiENSGT00530000062967.
HOGENOMiHOG000230900.
HOVERGENiHBG003815.
InParanoidiO55176.
KOiK10633.
OMAiVATELPC.
OrthoDBiEOG091G0306.
PhylomeDBiO55176.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR031227. PJA1.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
PANTHERiPTHR15710:SF2. PTHR15710:SF2. 1 hit.
PfamiView protein in Pfam
PF13639. zf-RING_2. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
PROSITEiView protein in PROSITE
PS50089. ZF_RING_2. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Alternative splicing appears to be tissue-specific.
Isoform 1 (identifier: O55176-3) [UniParc]FASTAAdd to basket
Also known as: Praja1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSHQERIASQ RRTTAEVPMH RSTANQSKRS RSPFASTRRR WDDSESSGAS
60 70 80 90 100
LAVESEDYSR YPPREYRASG SRRGLAYGHI DTVVARDSEE EGAGPVDRLP
110 120 130 140 150
VRGKAGKFKD DPEKGARSSR FTSVNHDAKE ECGKVESPPA ARCSARRAEL
160 170 180 190 200
SKQNGSSASQ ISSAEGRAAA KGNNSLERER QNLPARPSRA PVSICGGGEN
210 220 230 240 250
TPKSAEEPVV RPKVRNVATP NCMKPKVFFD TDDDDDVPHS TSRWRDAADA
260 270 280 290 300
EEAHAEGLAR RGRGEAASSS EPRYAEDQDA RSEQAKADKV PRRRRTMADP
310 320 330 340 350
DFWAYTDDYY RYYEEDSDSD KEWMAALRRK YRSREQPQSS SGESWELLPG
360 370 380 390 400
KEELERQQAG AGSLASAGSN GSGYPEEVQD PSLQEEEQAS LEEGEIPWLR
410 420 430 440 450
YNENESSSEG DNESTHELIQ PGMFMLDGNN NLEDDSSVSE DLEVDWSLFD
460 470 480 490 500
GFADGLGVAE AISYVDPQFL TYMALEERLA QAMETALAHL ESLAVDVEVA
510 520 530 540 550
NPPASKESID ALPEILVTED HGAVGQEMCC PICCSEYVKG EVATELPCHH
560 570
YFHKPCVSIW LQKSGTCPVC RCMFPPPL
Length:578
Mass (Da):63,906
Last modified:December 12, 2006 - v3
Checksum:i11B2D3C1F8982143
GO
Isoform 2 (identifier: O55176-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     60-243: Missing.

Show »
Length:394
Mass (Da):44,066
Checksum:iE7079C43B283ABA4
GO
Isoform 3 (identifier: O55176-4) [UniParc]FASTAAdd to basket
Also known as: Praja1c

The sequence of this isoform differs from the canonical sequence as follows:
     197-411: Missing.

Show »
Length:363
Mass (Da):39,676
Checksum:i8CFD7C8983F5B90A
GO
Isoform 4 (identifier: O55176-5) [UniParc]FASTAAdd to basket
Also known as: Praja1d

The sequence of this isoform differs from the canonical sequence as follows:
     185-187: ARP → PLF
     188-578: Missing.

Show »
Length:187
Mass (Da):20,548
Checksum:i54FFD305EA13E2FE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti236D → DD in AAK15764 (PubMed:11533224).Curated1
Sequence conflicti515I → M in BAB23982 (PubMed:16141072).Curated1
Sequence conflicti523A → T in AAK15764 (PubMed:11533224).Curated1
Sequence conflicti523A → T in AAK15765 (PubMed:11533224).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00751960 – 243Missing in isoform 2. 1 PublicationAdd BLAST184
Alternative sequenceiVSP_022011185 – 187ARP → PLF in isoform 4. 1 Publication3
Alternative sequenceiVSP_007520188 – 578Missing in isoform 4. 1 PublicationAdd BLAST391
Alternative sequenceiVSP_007521197 – 411Missing in isoform 3. 1 PublicationAdd BLAST215

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06944 mRNA. Translation: AAC00205.2.
AF335250 mRNA. Translation: AAK15764.2.
AF335251 mRNA. Translation: AAK15765.2.
AF335252 mRNA. Translation: AAK15766.1.
AK005373 mRNA. Translation: BAB23982.1.
BC037616 mRNA. Translation: AAH37616.1.
CCDSiCCDS41070.1. [O55176-3]
RefSeqiNP_001076579.1. NM_001083110.2. [O55176-3]
NP_032879.2. NM_008853.3.
UniGeneiMm.8211.

Genome annotation databases

EnsembliENSMUST00000036354; ENSMUSP00000109420; ENSMUSG00000034403. [O55176-3]
ENSMUST00000113792; ENSMUSP00000109423; ENSMUSG00000034403. [O55176-3]
ENSMUST00000167246; ENSMUSP00000132393; ENSMUSG00000034403. [O55176-3]
GeneIDi18744.
KEGGimmu:18744.
UCSCiuc009tvl.1. mouse. [O55176-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPJA1_MOUSE
AccessioniPrimary (citable) accession number: O55176
Secondary accession number(s): Q8CFU2
, Q99MJ1, Q99MJ2, Q99MJ3, Q9DB04
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: December 12, 2006
Last modified: October 25, 2017
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot