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Protein

Acyl-coenzyme A thioesterase 2, mitochondrial

Gene

Acot2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Most active on substrates with chain lengths ranging from C14-C20.

Catalytic activityi

Palmitoyl-CoA + H2O = CoA + palmitate.

pH dependencei

Optimum pH is 8-9.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei273Charge relay systemBy similarity1
Active sitei365Charge relay systemBy similarity1
Active sitei399Charge relay systemBy similarity1

GO - Molecular functioni

  • acyl-CoA hydrolase activity Source: RGD
  • carboxylic ester hydrolase activity Source: UniProtKB-KW
  • palmitoyl-CoA hydrolase activity Source: RGD

GO - Biological processi

  • acyl-CoA metabolic process Source: RGD
  • response to drug Source: RGD
  • response to epidermal growth factor Source: RGD
  • response to hormone Source: RGD
  • response to hypoxia Source: HGNC
  • response to nutrient levels Source: RGD
  • very long-chain fatty acid catabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

BRENDAi3.1.2.2. 5301.
3.1.2.20. 5301.

Protein family/group databases

ESTHERiratno-acot2. Acyl-CoA_Thioesterase.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A thioesterase 2, mitochondrial (EC:3.1.2.2)
Short name:
Acyl-CoA thioesterase 2
Alternative name(s):
ARTISt/p43
Acyl coenzyme A thioester hydrolase
MTE-I
Very-long-chain acyl-CoA thioesterase
Gene namesi
Name:Acot2
Synonyms:Mte1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621055. Acot2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 42MitochondrionSequence analysisAdd BLAST42
ChainiPRO_000003406643 – 453Acyl-coenzyme A thioesterase 2, mitochondrialAdd BLAST411

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei83N6-acetyllysineBy similarity1
Modified residuei447N6-succinyllysineBy similarity1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO55171.
PRIDEiO55171.

PTM databases

iPTMnetiO55171.
PhosphoSitePlusiO55171.

Expressioni

Tissue specificityi

Constitutively expressed in heart and brown fat. Strongly induced in liver, and weakly in kidney, in peroxisome proliferator treated rat.

Inductioni

Regulated by peroxisome proliferator, via the peroxisome proliferator-activated receptors (PPARs).

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013515.

Structurei

3D structure databases

ProteinModelPortaliO55171.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the C/M/P thioester hydrolase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410II3X. Eukaryota.
COG1073. LUCA.
HOGENOMiHOG000116219.
HOVERGENiHBG000331.
InParanoidiO55171.
PhylomeDBiO55171.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR016662. Acyl-CoA_thioEstase_long-chain.
IPR014940. BAAT_C.
IPR006862. Thio_Ohase/aa_AcTrfase.
[Graphical view]
PfamiPF08840. BAAT_C. 1 hit.
PF04775. Bile_Hydr_Trans. 1 hit.
[Graphical view]
PIRSFiPIRSF016521. Acyl-CoA_hydro. 1 hit.
SUPFAMiSSF53474. SSF53474. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55171-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVASSFAVLR ASRLCQWGWK SWTQLSGPPP LSTGGRTTFA RTNATLSLEP
60 70 80 90 100
GSRSCWDEPL SITVRGLAPE QPVTLRAALR DEKGALFRAH ARYRADAGGE
110 120 130 140 150
LDLARAPALG GSFTGLEPMG LIWAMEPERP LWRLVKRDVQ KPYVVELEVL
160 170 180 190 200
DGHEPDGGQR LAQAVHERHF MAPGVRRVPV RDGRVRATLF LPPEPGPFPE
210 220 230 240 250
IIDLFGVGGG LLEYRASLLA GKGFAVMALA YYNYDDLPKT METMRIEYFE
260 270 280 290 300
EAVNYLRGHP EVKGPGIGLL GISKGGELGL AMASFLKGIT AAVVINGSVA
310 320 330 340 350
AVGNTVCYKD ETIPPVSLLR DKVKMTKDGL LDVVEALQSP LVDKKSFIPV
360 370 380 390 400
ERSDTTFLFL VGQDDHNWKS EFYAREASKR LQAHGKEKPQ IICYPEAGHY
410 420 430 440 450
IEPPYFPLCS AGMHLLVGAN ITFGGEPKPH SVAQLDAWQQ LQTFFHKQLS

GKS
Length:453
Mass (Da):49,701
Last modified:June 1, 1998 - v1
Checksum:iF48C2C61475072B2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51 – 52GS → AG in BAA32539 (PubMed:9703974).Curated2
Sequence conflicti90 – 92HAR → RAL in BAA32539 (PubMed:9703974).Curated3
Sequence conflicti123W → R in BAA32539 (PubMed:9703974).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09333 mRNA. Translation: CAA70513.1.
AB010429 mRNA. Translation: BAA32539.1.
UniGeneiRn.37524.

Genome annotation databases

UCSCiRGD:621055. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09333 mRNA. Translation: CAA70513.1.
AB010429 mRNA. Translation: BAA32539.1.
UniGeneiRn.37524.

3D structure databases

ProteinModelPortaliO55171.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013515.

Protein family/group databases

ESTHERiratno-acot2. Acyl-CoA_Thioesterase.

PTM databases

iPTMnetiO55171.
PhosphoSitePlusiO55171.

Proteomic databases

PaxDbiO55171.
PRIDEiO55171.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:621055. rat.

Organism-specific databases

RGDi621055. Acot2.

Phylogenomic databases

eggNOGiENOG410II3X. Eukaryota.
COG1073. LUCA.
HOGENOMiHOG000116219.
HOVERGENiHBG000331.
InParanoidiO55171.
PhylomeDBiO55171.

Enzyme and pathway databases

BRENDAi3.1.2.2. 5301.
3.1.2.20. 5301.

Miscellaneous databases

PROiO55171.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR016662. Acyl-CoA_thioEstase_long-chain.
IPR014940. BAAT_C.
IPR006862. Thio_Ohase/aa_AcTrfase.
[Graphical view]
PfamiPF08840. BAAT_C. 1 hit.
PF04775. Bile_Hydr_Trans. 1 hit.
[Graphical view]
PIRSFiPIRSF016521. Acyl-CoA_hydro. 1 hit.
SUPFAMiSSF53474. SSF53474. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiACOT2_RAT
AccessioniPrimary (citable) accession number: O55171
Secondary accession number(s): O88268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.