Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Epithelial cell adhesion molecule

Gene

Epcam

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).By similarity

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: RGD

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Epithelial cell adhesion molecule
Short name:
Ep-CAM
Alternative name(s):
Epithelial glycoprotein 314
Short name:
EGP314
Protein D5.7A
Tumor-associated calcium signal transducer 1
CD_antigen: CD326
Gene namesi
Name:Epcam
Synonyms:Tacstd1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi621365. Epcam.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 266243ExtracellularSequence analysisAdd
BLAST
Transmembranei267 – 28923HelicalSequence analysisAdd
BLAST
Topological domaini290 – 31526CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: Ensembl
  • basolateral plasma membrane Source: Ensembl
  • bicellular tight junction Source: UniProtKB
  • cell surface Source: Ensembl
  • extracellular exosome Source: Ensembl
  • integral component of membrane Source: RGD
  • lateral plasma membrane Source: UniProtKB
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 315292Epithelial cell adhesion moleculePRO_0000380185Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 46PROSITE-ProRule annotation
Disulfide bondi29 ↔ 59PROSITE-ProRule annotation
Disulfide bondi38 ↔ 48PROSITE-ProRule annotation
Disulfide bondi66 ↔ 99PROSITE-ProRule annotation
Disulfide bondi110 ↔ 116PROSITE-ProRule annotation
Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence analysis
Disulfide bondi118 ↔ 135PROSITE-ProRule annotation
Glycosylationi198 – 1981N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylation at Asn-198 is crucial for protein stability.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO55159.
PRIDEiO55159.

PTM databases

iPTMnetiO55159.

Expressioni

Gene expression databases

GenevisibleiO55159. RN.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with phosphorylated CLDN7.By similarity

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021135.

Structurei

3D structure databases

ProteinModelPortaliO55159.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 13573Thyroglobulin type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the EPCAM family.Curated
Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFI6. Eukaryota.
ENOG4111M3B. LUCA.
GeneTreeiENSGT00390000018245.
HOGENOMiHOG000074086.
InParanoidiO55159.
KOiK06737.
OMAiTTCWCVN.
OrthoDBiEOG7N0C5D.
PhylomeDBiO55159.
TreeFamiTF332767.

Family and domain databases

Gene3Di4.10.800.10. 1 hit.
InterProiIPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
SMARTiSM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57610. SSF57610. 1 hit.
PROSITEiPS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55159-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPKALAFG LLLAVVTATL AAAQKDCVCN NYKLTSRCYE NENGECQCTS
60 70 80 90 100
YGTQNTVICS KLASKCLVMK AEMTHSKSGR RMKPEGAIQN NDGLYDPECD
110 120 130 140 150
EQGLFKAKQC NGTATCWCVN TAGVRRTDKD TEITCSERVR TYWIIIELKH
160 170 180 190 200
KERAQPYNFE SLHTALQDTF ASRYMLNPKF IKSIMYENNV ITIDLMQNSS
210 220 230 240 250
QKTQDDVDIA DVAYYFEKDV KGESLFHSSK SMDLRVNGEL LDLDPGQTLI
260 270 280 290 300
YYVDEKAPEF SMQGLTAGII AVIVVVVLAV IAGIVVLVIS TRKRSAKYEK
310
AEIKEMGEIH RELNA
Length:315
Mass (Da):35,207
Last modified:June 1, 1998 - v1
Checksum:i024F796B18F85BC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001044 mRNA. Translation: CAA04498.1.
CH473947 Genomic DNA. Translation: EDM02636.1.
BC072691 mRNA. Translation: AAH72691.1.
RefSeqiNP_612550.1. NM_138541.1.
UniGeneiRn.106481.

Genome annotation databases

EnsembliENSRNOT00000021135; ENSRNOP00000021135; ENSRNOG00000015667.
GeneIDi171577.
KEGGirno:171577.
UCSCiRGD:621365. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001044 mRNA. Translation: CAA04498.1.
CH473947 Genomic DNA. Translation: EDM02636.1.
BC072691 mRNA. Translation: AAH72691.1.
RefSeqiNP_612550.1. NM_138541.1.
UniGeneiRn.106481.

3D structure databases

ProteinModelPortaliO55159.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021135.

PTM databases

iPTMnetiO55159.

Proteomic databases

PaxDbiO55159.
PRIDEiO55159.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021135; ENSRNOP00000021135; ENSRNOG00000015667.
GeneIDi171577.
KEGGirno:171577.
UCSCiRGD:621365. rat.

Organism-specific databases

CTDi4072.
RGDi621365. Epcam.

Phylogenomic databases

eggNOGiENOG410IFI6. Eukaryota.
ENOG4111M3B. LUCA.
GeneTreeiENSGT00390000018245.
HOGENOMiHOG000074086.
InParanoidiO55159.
KOiK06737.
OMAiTTCWCVN.
OrthoDBiEOG7N0C5D.
PhylomeDBiO55159.
TreeFamiTF332767.

Miscellaneous databases

NextBioi622612.
PROiO55159.

Gene expression databases

GenevisibleiO55159. RN.

Family and domain databases

Gene3Di4.10.800.10. 1 hit.
InterProiIPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
SMARTiSM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57610. SSF57610. 1 hit.
PROSITEiPS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Metastasis-association of the rat ortholog of the human epithelial glycoprotein antigen EGP314."
    Wuerfel J., Roesel M., Seiter S., Claas C., Herlevsen M., Weth R., Zoeller M.
    Oncogene 18:2323-2334(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BDIX.
    Tissue: Colon carcinoma.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  4. "The cell-cell adhesion molecule EpCAM interacts directly with the tight junction protein claudin-7."
    Ladwein M., Pape U.F., Schmidt D.S., Schnoelzer M., Fiedler S., Langbein L., Franke W.W., Moldenhauer G., Zoeller M.
    Exp. Cell Res. 309:345-357(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CLDN7.

Entry informationi

Entry nameiEPCAM_RAT
AccessioniPrimary (citable) accession number: O55159
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 1, 1998
Last modified: April 13, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.