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O55145

- X3CL1_RAT

UniProt

O55145 - X3CL1_RAT

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Protein

Fractalkine

Gene

Cx3cl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei335 – 3362Cleavage; to produce soluble formSequence Analysis

GO - Molecular functioni

  1. chemokine activity Source: RGD

GO - Biological processi

  1. cell adhesion Source: UniProtKB-KW
  2. cell chemotaxis Source: GOC
  3. chemotaxis Source: RGD
  4. immune response Source: InterPro
  5. negative regulation of cytokine secretion Source: RGD
  6. negative regulation of vasoconstriction Source: RGD
  7. platelet activation Source: RGD
  8. positive regulation of cell adhesion Source: RGD
  9. positive regulation of cell migration Source: RGD
  10. positive regulation of macrophage chemotaxis Source: RGD
  11. positive regulation of smooth muscle cell proliferation Source: RGD
  12. regulation of inhibitory postsynaptic membrane potential Source: RGD
  13. response to hypoxia Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Cell adhesion, Chemotaxis

Names & Taxonomyi

Protein namesi
Recommended name:
Fractalkine
Alternative name(s):
C-X3-C motif chemokine 1
CX3C membrane-anchored chemokine
Neurotactin
Small-inducible cytokine D1
Cleaved into the following chain:
Gene namesi
Name:Cx3cl1
Synonyms:Acc1, Fkn, Scyd1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620458. Cx3cl1.

Subcellular locationi

GO - Cellular componenti

  1. cell projection Source: RGD
  2. cytoplasm Source: RGD
  3. extracellular space Source: UniProtKB-KW
  4. integral component of membrane Source: UniProtKB-KW
  5. perinuclear region of cytoplasm Source: RGD
  6. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 393369FractalkinePRO_0000005254Add
BLAST
Chaini25 – ?335311Processed fractalkinePRO_0000296226Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 58By similarity
Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi36 ↔ 74By similarity

Post-translational modificationi

A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiO55145.

Expressioni

Tissue specificityi

Highest levels in brain (neurons). Significant levels in kidney, heart, lung and adrenal gland.

Gene expression databases

GenevestigatoriO55145.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi250131. 1 interaction.
STRINGi10116.ENSRNOP00000022128.

Structurei

3D structure databases

ProteinModelPortaliO55145.
SMRiO55145. Positions 25-100.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 337313ExtracellularSequence AnalysisAdd
BLAST
Topological domaini359 – 39335CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei338 – 35821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 10076ChemokineAdd
BLAST
Regioni101 – 337237Mucin-like stalkAdd
BLAST

Sequence similaritiesi

Belongs to the intercrine delta family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG46337.
HOGENOMiHOG000036946.
HOVERGENiHBG057269.
InParanoidiO55145.
KOiK05508.
PhylomeDBiO55145.

Family and domain databases

InterProiIPR008097. Chemokine_CX3CL1.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR01721. FRACTALKINE.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55145-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPSQLAWLL RLAAFFHLCT LLAGQHLGMT KCNITCHKMT SPIPVTLLIH
60 70 80 90 100
YQLNQESCGK RAIILETRQH RHFCADPKEK WVQDAMKHLD HQTAALTRNG
110 120 130 140 150
GKFEKRVDNV TPRITSATRG LSPTALAKPE SATVEDLTLE PTAISQEARR
160 170 180 190 200
PMGTSQEPPA AVTGSSPSTS KAQDAGLAAK PQSTGISEVA AVSTTIWPSS
210 220 230 240 250
AVYQSGSSLW AEEKATESPP TIALSTQAST TSSPKQNVGS EGQPPWVQEQ
260 270 280 290 300
DSTPEKSPGP EETNPVHTDI FQDRGPGSTV HPSVAPTSSE KTPSPELVAS
310 320 330 340 350
GSQAPKVEEP IHATADPQKL SVFITPVPDS QAATRRQAVG LLAFLGLLFC
360 370 380 390
LGVAMFAYQS LQGCPRKMAG EMVEGLRYVP RSCGSNSYVL VPV
Length:393
Mass (Da):41,977
Last modified:August 14, 2001 - v3
Checksum:iF06BB027CBD8CE14
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF030358 mRNA. Translation: AAC33834.2.
Y16813 mRNA. Translation: CAA76404.1.
RefSeqiNP_604450.1. NM_134455.1.
UniGeneiRn.107266.

Genome annotation databases

GeneIDi89808.
KEGGirno:89808.
UCSCiRGD:620458. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF030358 mRNA. Translation: AAC33834.2 .
Y16813 mRNA. Translation: CAA76404.1 .
RefSeqi NP_604450.1. NM_134455.1.
UniGenei Rn.107266.

3D structure databases

ProteinModelPortali O55145.
SMRi O55145. Positions 25-100.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 250131. 1 interaction.
STRINGi 10116.ENSRNOP00000022128.

Proteomic databases

PRIDEi O55145.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 89808.
KEGGi rno:89808.
UCSCi RGD:620458. rat.

Organism-specific databases

CTDi 6376.
RGDi 620458. Cx3cl1.

Phylogenomic databases

eggNOGi NOG46337.
HOGENOMi HOG000036946.
HOVERGENi HBG057269.
InParanoidi O55145.
KOi K05508.
PhylomeDBi O55145.

Miscellaneous databases

NextBioi 617668.
PROi O55145.

Gene expression databases

Genevestigatori O55145.

Family and domain databases

InterProi IPR008097. Chemokine_CX3CL1.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view ]
Pfami PF00048. IL8. 1 hit.
[Graphical view ]
PRINTSi PR01721. FRACTALKINE.
SMARTi SM00199. SCY. 1 hit.
[Graphical view ]
SUPFAMi SSF54117. SSF54117. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Role for neuronally derived fractalkine in mediating interactions between neurons and CX3CR1-expressing microglia."
    Harrison J.K., Jiang Y., Chen S., Xia Y., Maciejewski D., McNamara R.K., Streit W.J., Salafranca M.N., Adhikari S., Thompson D.A., Botti P., Bacon K.B., Feng L.
    Proc. Natl. Acad. Sci. U.S.A. 95:10896-10901(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. Feng L., Harrison J.K.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 127-151.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 204-335.

Entry informationi

Entry nameiX3CL1_RAT
AccessioniPrimary (citable) accession number: O55145
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 14, 2001
Last modified: October 29, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3