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O55145

- X3CL1_RAT

UniProt

O55145 - X3CL1_RAT

Protein

Fractalkine

Gene

Cx3cl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 3 (14 Aug 2001)
      Previous versions | rss
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    Functioni

    The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei335 – 3362Cleavage; to produce soluble formSequence Analysis

    GO - Molecular functioni

    1. chemokine activity Source: RGD

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW
    2. cell chemotaxis Source: GOC
    3. chemotaxis Source: RGD
    4. immune response Source: InterPro
    5. negative regulation of cytokine secretion Source: RGD
    6. negative regulation of vasoconstriction Source: RGD
    7. platelet activation Source: RGD
    8. positive regulation of cell adhesion Source: RGD
    9. positive regulation of cell migration Source: RGD
    10. positive regulation of macrophage chemotaxis Source: RGD
    11. positive regulation of smooth muscle cell proliferation Source: RGD
    12. regulation of inhibitory postsynaptic membrane potential Source: RGD
    13. response to hypoxia Source: RGD

    Keywords - Molecular functioni

    Cytokine

    Keywords - Biological processi

    Cell adhesion, Chemotaxis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fractalkine
    Alternative name(s):
    C-X3-C motif chemokine 1
    CX3C membrane-anchored chemokine
    Neurotactin
    Small-inducible cytokine D1
    Cleaved into the following chain:
    Gene namesi
    Name:Cx3cl1
    Synonyms:Acc1, Fkn, Scyd1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620458. Cx3cl1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell projection Source: RGD
    2. cytoplasm Source: RGD
    3. extracellular space Source: UniProtKB-KW
    4. integral component of membrane Source: UniProtKB-KW
    5. perinuclear region of cytoplasm Source: RGD
    6. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 393369FractalkinePRO_0000005254Add
    BLAST
    Chaini25 – ?335311Processed fractalkinePRO_0000296226Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi32 ↔ 58By similarity
    Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi36 ↔ 74By similarity

    Post-translational modificationi

    A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiO55145.

    Expressioni

    Tissue specificityi

    Highest levels in brain (neurons). Significant levels in kidney, heart, lung and adrenal gland.

    Gene expression databases

    GenevestigatoriO55145.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    BioGridi250131. 1 interaction.
    STRINGi10116.ENSRNOP00000022128.

    Structurei

    3D structure databases

    ProteinModelPortaliO55145.
    SMRiO55145. Positions 25-100.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini25 – 337313ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini359 – 39335CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei338 – 35821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni25 – 10076ChemokineAdd
    BLAST
    Regioni101 – 337237Mucin-like stalkAdd
    BLAST

    Sequence similaritiesi

    Belongs to the intercrine delta family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG46337.
    HOGENOMiHOG000036946.
    HOVERGENiHBG057269.
    InParanoidiO55145.
    KOiK05508.
    PhylomeDBiO55145.

    Family and domain databases

    InterProiIPR008097. Chemokine_CX3CL1.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view]
    PfamiPF00048. IL8. 1 hit.
    [Graphical view]
    PRINTSiPR01721. FRACTALKINE.
    SMARTiSM00199. SCY. 1 hit.
    [Graphical view]
    SUPFAMiSSF54117. SSF54117. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O55145-1 [UniParc]FASTAAdd to Basket

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    MAPSQLAWLL RLAAFFHLCT LLAGQHLGMT KCNITCHKMT SPIPVTLLIH    50
    YQLNQESCGK RAIILETRQH RHFCADPKEK WVQDAMKHLD HQTAALTRNG 100
    GKFEKRVDNV TPRITSATRG LSPTALAKPE SATVEDLTLE PTAISQEARR 150
    PMGTSQEPPA AVTGSSPSTS KAQDAGLAAK PQSTGISEVA AVSTTIWPSS 200
    AVYQSGSSLW AEEKATESPP TIALSTQAST TSSPKQNVGS EGQPPWVQEQ 250
    DSTPEKSPGP EETNPVHTDI FQDRGPGSTV HPSVAPTSSE KTPSPELVAS 300
    GSQAPKVEEP IHATADPQKL SVFITPVPDS QAATRRQAVG LLAFLGLLFC 350
    LGVAMFAYQS LQGCPRKMAG EMVEGLRYVP RSCGSNSYVL VPV 393
    Length:393
    Mass (Da):41,977
    Last modified:August 14, 2001 - v3
    Checksum:iF06BB027CBD8CE14
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF030358 mRNA. Translation: AAC33834.2.
    Y16813 mRNA. Translation: CAA76404.1.
    RefSeqiNP_604450.1. NM_134455.1.
    UniGeneiRn.107266.

    Genome annotation databases

    GeneIDi89808.
    KEGGirno:89808.
    UCSCiRGD:620458. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF030358 mRNA. Translation: AAC33834.2 .
    Y16813 mRNA. Translation: CAA76404.1 .
    RefSeqi NP_604450.1. NM_134455.1.
    UniGenei Rn.107266.

    3D structure databases

    ProteinModelPortali O55145.
    SMRi O55145. Positions 25-100.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250131. 1 interaction.
    STRINGi 10116.ENSRNOP00000022128.

    Proteomic databases

    PRIDEi O55145.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 89808.
    KEGGi rno:89808.
    UCSCi RGD:620458. rat.

    Organism-specific databases

    CTDi 6376.
    RGDi 620458. Cx3cl1.

    Phylogenomic databases

    eggNOGi NOG46337.
    HOGENOMi HOG000036946.
    HOVERGENi HBG057269.
    InParanoidi O55145.
    KOi K05508.
    PhylomeDBi O55145.

    Miscellaneous databases

    NextBioi 617668.
    PROi O55145.

    Gene expression databases

    Genevestigatori O55145.

    Family and domain databases

    InterProi IPR008097. Chemokine_CX3CL1.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view ]
    Pfami PF00048. IL8. 1 hit.
    [Graphical view ]
    PRINTSi PR01721. FRACTALKINE.
    SMARTi SM00199. SCY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54117. SSF54117. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Role for neuronally derived fractalkine in mediating interactions between neurons and CX3CR1-expressing microglia."
      Harrison J.K., Jiang Y., Chen S., Xia Y., Maciejewski D., McNamara R.K., Streit W.J., Salafranca M.N., Adhikari S., Thompson D.A., Botti P., Bacon K.B., Feng L.
      Proc. Natl. Acad. Sci. U.S.A. 95:10896-10901(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. Feng L., Harrison J.K.
      Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 127-151.
    3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 204-335.

    Entry informationi

    Entry nameiX3CL1_RAT
    AccessioniPrimary (citable) accession number: O55145
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: August 14, 2001
    Last modified: October 1, 2014
    This is version 100 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3