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O55145 (X3CL1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fractalkine
Alternative name(s):
C-X3-C motif chemokine 1
CX3C membrane-anchored chemokine
Neurotactin
Small-inducible cytokine D1

Cleaved into the following chain:

  1. Processed fractalkine
Gene names
Name:Cx3cl1
Synonyms:Acc1, Fkn, Scyd1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.

Subunit structure

Monomer By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity.

Processed fractalkine: Secreted By similarity.

Tissue specificity

Highest levels in brain (neurons). Significant levels in kidney, heart, lung and adrenal gland.

Post-translational modification

A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.

Sequence similarities

Belongs to the intercrine delta family.

Ontologies

Keywords
   Biological processCell adhesion
Chemotaxis
   Cellular componentCell membrane
Membrane
Secreted
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionCytokine
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

cell chemotaxis

Inferred from direct assay Ref.1. Source: GOC

chemotaxis

Inferred from direct assay Ref.1. Source: RGD

immune response

Inferred from electronic annotation. Source: InterPro

negative regulation of cytokine secretion

Inferred from mutant phenotype PubMed 10652441. Source: RGD

negative regulation of vasoconstriction

Inferred from direct assay PubMed 19201996. Source: RGD

platelet activation

Inferred from mutant phenotype PubMed 12969973. Source: RGD

positive regulation of cell adhesion

Inferred from direct assay PubMed 17114429. Source: RGD

positive regulation of cell migration

Inferred from direct assay PubMed 17114429. Source: RGD

positive regulation of macrophage chemotaxis

Inferred from mutant phenotype PubMed 19282612. Source: RGD

positive regulation of smooth muscle cell proliferation

Inferred from direct assay PubMed 17182651. Source: RGD

regulation of inhibitory postsynaptic membrane potential

Inferred from mutant phenotype PubMed 19748551. Source: RGD

response to hypoxia

Inferred from expression pattern PubMed 18630689. Source: RGD

   Cellular_componentcell projection

Inferred from direct assay PubMed 11870871. Source: RGD

cytoplasm

Inferred from direct assay PubMed 19748551. Source: RGD

extracellular space

Inferred from electronic annotation. Source: UniProtKB-KW

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

perinuclear region of cytoplasm

Inferred from direct assay PubMed 11870871. Source: RGD

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionchemokine activity

Inferred from direct assay Ref.1. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 393369Fractalkine
PRO_0000005254
Chain25 – ?335311Processed fractalkine
PRO_0000296226

Regions

Topological domain25 – 337313Extracellular Potential
Transmembrane338 – 35821Helical; Potential
Topological domain359 – 39335Cytoplasmic Potential
Region25 – 10076Chemokine
Region101 – 337237Mucin-like stalk

Sites

Site335 – 3362Cleavage; to produce soluble form Potential

Amino acid modifications

Glycosylation331N-linked (GlcNAc...) Potential
Disulfide bond32 ↔ 58 By similarity
Disulfide bond36 ↔ 74 By similarity

Sequences

Sequence LengthMass (Da)Tools
O55145 [UniParc].

Last modified August 14, 2001. Version 3.
Checksum: F06BB027CBD8CE14

FASTA39341,977
        10         20         30         40         50         60 
MAPSQLAWLL RLAAFFHLCT LLAGQHLGMT KCNITCHKMT SPIPVTLLIH YQLNQESCGK 

        70         80         90        100        110        120 
RAIILETRQH RHFCADPKEK WVQDAMKHLD HQTAALTRNG GKFEKRVDNV TPRITSATRG 

       130        140        150        160        170        180 
LSPTALAKPE SATVEDLTLE PTAISQEARR PMGTSQEPPA AVTGSSPSTS KAQDAGLAAK 

       190        200        210        220        230        240 
PQSTGISEVA AVSTTIWPSS AVYQSGSSLW AEEKATESPP TIALSTQAST TSSPKQNVGS 

       250        260        270        280        290        300 
EGQPPWVQEQ DSTPEKSPGP EETNPVHTDI FQDRGPGSTV HPSVAPTSSE KTPSPELVAS 

       310        320        330        340        350        360 
GSQAPKVEEP IHATADPQKL SVFITPVPDS QAATRRQAVG LLAFLGLLFC LGVAMFAYQS 

       370        380        390 
LQGCPRKMAG EMVEGLRYVP RSCGSNSYVL VPV 

« Hide

References

[1]"Role for neuronally derived fractalkine in mediating interactions between neurons and CX3CR1-expressing microglia."
Harrison J.K., Jiang Y., Chen S., Xia Y., Maciejewski D., McNamara R.K., Streit W.J., Salafranca M.N., Adhikari S., Thompson D.A., Botti P., Bacon K.B., Feng L.
Proc. Natl. Acad. Sci. U.S.A. 95:10896-10901(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]Feng L., Harrison J.K.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 127-151.
[3]"Neuronal expression of fractalkine in the presence and absence of inflammation."
Schwaeble W.J., Stover C.M., Schall T.J., Dairaghi D.J., Trinder P.K.E., Linington C., Iglesias A., Schubart A., Lynch N.J., Weihe E., Schaefer M.K.-H.
FEBS Lett. 439:203-207(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 204-335.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF030358 mRNA. Translation: AAC33834.2.
Y16813 mRNA. Translation: CAA76404.1.
RefSeqNP_604450.1. NM_134455.1.
UniGeneRn.107266.

3D structure databases

ProteinModelPortalO55145.
SMRO55145. Positions 25-100.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid250131. 1 interaction.
STRING10116.ENSRNOP00000022128.

Proteomic databases

PRIDEO55145.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID89808.
KEGGrno:89808.
UCSCRGD:620458. rat.

Organism-specific databases

CTD6376.
RGD620458. Cx3cl1.

Phylogenomic databases

eggNOGNOG46337.
HOGENOMHOG000036946.
HOVERGENHBG057269.
InParanoidO55145.
KOK05508.
PhylomeDBO55145.

Gene expression databases

GenevestigatorO55145.

Family and domain databases

InterProIPR008097. Chemokine_CX3CL1.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamPF00048. IL8. 1 hit.
[Graphical view]
PRINTSPR01721. FRACTALKINE.
SMARTSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMSSF54117. SSF54117. 1 hit.
ProtoNetSearch...

Other

NextBio617668.
PROO55145.

Entry information

Entry nameX3CL1_RAT
AccessionPrimary (citable) accession number: O55145
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 14, 2001
Last modified: April 16, 2014
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families