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Protein

Acyl-coenzyme A thioesterase 1

Gene

Acot1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. True acyl-CoA thioesterase being highly specific for saturated C12-C20 acyl-CoAs, with only a very low activity with decanoyl-CoA as substrate. Most active on myristoyl- and palmitoyl-CoA. Introduction of one or two double bonds decreases the activity to about half. No detectable activity with bile acid CoAs such as choloyl-CoA and chenodeoxycholoyl-CoA.1 Publication

Catalytic activityi

Palmitoyl-CoA + H2O = CoA + palmitate.

Kineticsi

  1. KM=2.6 µM for palmitoyl-CoA1 Publication
  1. Vmax=1.2 µmol/min/mg enzyme with palmitoyl-CoA as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei232Charge relay system1 Publication1
Active sitei324Charge relay system1 Publication1
Active sitei358Charge relay system1 Publication1

GO - Molecular functioni

GO - Biological processi

  • acyl-CoA metabolic process Source: MGI
  • long-chain fatty acid metabolic process Source: MGI

Keywordsi

Molecular functionHydrolase, Serine esterase

Enzyme and pathway databases

BRENDAi3.1.2.2 3474
ReactomeiR-MMU-77289 Mitochondrial Fatty Acid Beta-Oxidation
R-MMU-9033241 Peroxisomal protein import
SABIO-RKiO55137

Protein family/group databases

ESTHERimouse-acot1 Acyl-CoA_Thioesterase

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A thioesterase 1 (EC:3.1.2.2)
Short name:
Acyl-CoA thioesterase 1
Alternative name(s):
CTE-I
Inducible cytosolic acyl-coenzyme A thioester hydrolase
Long chain acyl-CoA thioester hydrolase
Short name:
Long chain acyl-CoA hydrolase
Gene namesi
Name:Acot1
Synonyms:Cte1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1349396 Acot1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi232S → A: Abolishes activity. 1 Publication1
Mutagenesisi232S → C: Retains 2% of the initial activity; deacylation of the acyl-enzyme intermediate becomes rate-limiting. 1 Publication1
Mutagenesisi324D → A: Abolishes activity. 1 Publication1
Mutagenesisi358H → Q: Abolishes activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3259489

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002021561 – 419Acyl-coenzyme A thioesterase 1Add BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei416PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO55137
PaxDbiO55137
PeptideAtlasiO55137
PRIDEiO55137

2D gel databases

UCD-2DPAGEiO55137

PTM databases

iPTMnetiO55137
PhosphoSitePlusiO55137

Expressioni

Tissue specificityi

Expressed in heart, kidney, brown adipose tissue, white adipose tissue, adrenal gland and muscle.2 Publications

Inductioni

In the liver, by peroxisome proliferator (Clofibrate) treatment, via the peroxisome proliferator-activated receptors (PPARs) or fasting for 24 hours.1 Publication

Gene expression databases

BgeeiENSMUSG00000072949
CleanExiMM_ACOT1
ExpressionAtlasiO55137 baseline and differential
GenevisibleiO55137 MM

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiO55137, 3 interactors
MINTiO55137
STRINGi10090.ENSMUSP00000126448

Structurei

3D structure databases

ProteinModelPortaliO55137
SMRiO55137
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the C/M/P thioester hydrolase family.Curated

Phylogenomic databases

eggNOGiENOG410II3X Eukaryota
COG1073 LUCA
GeneTreeiENSGT00390000001046
HOVERGENiHBG000331
InParanoidiO55137
KOiK01068
OMAiPETEIPW
OrthoDBiEOG091G08KU
PhylomeDBiO55137
TreeFamiTF314911

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR016662 Acyl-CoA_thioEstase_long-chain
IPR014940 BAAT_C
IPR006862 Thio_Ohase/aa_AcTrfase
PfamiView protein in Pfam
PF08840 BAAT_C, 1 hit
PF04775 Bile_Hydr_Trans, 1 hit
PIRSFiPIRSF016521 Acyl-CoA_hydro, 1 hit
SUPFAMiSSF53474 SSF53474, 2 hits

Sequencei

Sequence statusi: Complete.

O55137-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEATLNLEPS GRSCWDEPLS IAVRGLAPEQ PVTLRSVLRD EKGALFRAHA
60 70 80 90 100
RYRADSHGEL DLARTPALGG SFSGLEPMGL LWAMEPDRPF WRLVKRDVQT
110 120 130 140 150
PFVVELEVLD GHEPDGGQRL AHAVHERHFL APGVRRVPVR EGRVRATLFL
160 170 180 190 200
PPEPGPFPGI IDLFGVGGGL LEYRASLLAG KGFAVMALAY YNYDDLPKNM
210 220 230 240 250
ETMHMEYFEE AVNYLRSHPE VKGPGIGLLG ISKGGELGLA MASFLKGITA
260 270 280 290 300
AVVINGSVAA VGNTISYKDE TIPPVTILRN QVKMTKDGLK DVVDALQSPL
310 320 330 340 350
VDKKSFIPVE RSDTTFLFLV GQDDHNWKSE FYADEISKRL QAHGKEKPQI
360 370 380 390 400
ICYPAAGHYI EPPYFPLCSA GMHLLVGANI TFGGEPKPHA MAQLDAWQQL
410
QTFFHKQLGS ECLHVSPKI
Length:419
Mass (Da):46,136
Last modified:June 1, 1998 - v1
Checksum:i57346B6177471CFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF180795, AF180793, AF180794 Genomic DNA Translation: AAF13870.1
Y14004 mRNA Translation: CAA74327.1
BC028261 mRNA Translation: AAH28261.1
CCDSiCCDS36487.1
RefSeqiNP_036136.1, NM_012006.2
UniGeneiMm.1978

Genome annotation databases

EnsembliENSMUST00000168120; ENSMUSP00000126448; ENSMUSG00000072949
GeneIDi26897
KEGGimmu:26897
UCSCiuc007oeb.1 mouse

Similar proteinsi

Entry informationi

Entry nameiACOT1_MOUSE
AccessioniPrimary (citable) accession number: O55137
Secondary accession number(s): Q549A9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: June 20, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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