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Protein

Eukaryotic translation initiation factor 6

Gene

Eif6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Ribosome biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 6UniRule annotation
Short name:
eIF-6UniRule annotation
Alternative name(s):
B4 integrin interactor
CAB
p27(BBP)
Gene namesi
Name:Eif6
Synonyms:Itgb4bp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1196288. Eif6.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • intermediate filament Source: MGI
  • lamin filament Source: MGI
  • nucleolus Source: UniProtKB-SubCell
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality. Embryos die at preimplantation stage.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Eukaryotic translation initiation factor 6PRO_0000153735Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei113 – 1131PhosphotyrosineBy similarity
Modified residuei165 – 1651PhosphothreonineUniRule annotation1 Publication
Modified residuei166 – 1661PhosphoserineUniRule annotation1 Publication
Modified residuei174 – 1741Phosphoserine; by CK1UniRule annotation1 Publication
Modified residuei175 – 1751Phosphoserine; by CK1UniRule annotation1 Publication
Modified residuei235 – 2351Phosphoserine; by PKCUniRule annotationBy similarity
Modified residuei239 – 2391PhosphoserineUniRule annotationBy similarity
Modified residuei243 – 2431PhosphoserineUniRule annotationBy similarity

Post-translational modificationi

Phosphorylation at Ser-174 and Ser-175 by CSNK1D/CK1 promotes nuclear export.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO55135.
PaxDbiO55135.
PRIDEiO55135.

PTM databases

iPTMnetiO55135.
PhosphoSiteiO55135.
SwissPalmiO55135.

Expressioni

Tissue specificityi

Detected in bladder, duodenum, liver, esophagus, pancreas and testis with lower levels in muscle (at protein level).1 Publication

Gene expression databases

BgeeiO55135.
CleanExiMM_EIF6.
ExpressionAtlasiO55135. baseline and differential.
GenevisibleiO55135. MM.

Interactioni

Subunit structurei

Monomer. Associates with the 60S ribosomal subunit. Interacts with RACK1.UniRule annotation

Protein-protein interaction databases

BioGridi200831. 4 interactions.
IntActiO55135. 2 interactions.
STRINGi10090.ENSMUSP00000029142.

Structurei

3D structure databases

ProteinModelPortaliO55135.
SMRiO55135. Positions 1-224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-6 family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3185. Eukaryota.
COG1976. LUCA.
HOGENOMiHOG000230605.
HOVERGENiHBG001300.
InParanoidiO55135.
KOiK03264.
OMAiNDWCAFT.
OrthoDBiEOG7V4B05.
PhylomeDBiO55135.
TreeFamiTF105396.

Family and domain databases

HAMAPiMF_00032. eIF_6.
InterProiIPR002769. eIF6.
[Graphical view]
PANTHERiPTHR10784. PTHR10784. 1 hit.
PfamiPF01912. eIF-6. 1 hit.
[Graphical view]
PIRSFiPIRSF006413. IF-6. 1 hit.
SMARTiSM00654. eIF6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00323. eIF-6. 1 hit.

Sequencei

Sequence statusi: Complete.

O55135-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVRASFENN CEVGCFAKLT NAYCLVAIGG SENFYSVFEG ELSDAIPVVH
60 70 80 90 100
ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLPD SVQIRRVEER
110 120 130 140 150
LSALGNVTTC NDYVALVHPD LDRETEEILA DVLKVEVFRQ TVADQVLVGS
160 170 180 190 200
YCVFSNQGGL VHPKTSIEDQ DELSSLLQVP LVAGTVNRGS EVIAAGMVVN
210 220 230 240
DWCAFCGLDT TSTELSVVES VFKLNEAKPS TIATSMRDSL IDSLT
Length:245
Mass (Da):26,511
Last modified:May 30, 2000 - v2
Checksum:i35BF0F4C17D5E045
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047046 mRNA. Translation: AAD28078.1.
BC015274 mRNA. Translation: AAH15274.1.
BC024442 mRNA. Translation: AAH24442.1.
Y11460 mRNA. Translation: CAA72246.1.
CCDSiCCDS16956.1.
RefSeqiNP_034709.1. NM_010579.2.
UniGeneiMm.271674.

Genome annotation databases

EnsembliENSMUST00000029142; ENSMUSP00000029142; ENSMUSG00000027613.
GeneIDi16418.
KEGGimmu:16418.
UCSCiuc008nll.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047046 mRNA. Translation: AAD28078.1.
BC015274 mRNA. Translation: AAH15274.1.
BC024442 mRNA. Translation: AAH24442.1.
Y11460 mRNA. Translation: CAA72246.1.
CCDSiCCDS16956.1.
RefSeqiNP_034709.1. NM_010579.2.
UniGeneiMm.271674.

3D structure databases

ProteinModelPortaliO55135.
SMRiO55135. Positions 1-224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200831. 4 interactions.
IntActiO55135. 2 interactions.
STRINGi10090.ENSMUSP00000029142.

PTM databases

iPTMnetiO55135.
PhosphoSiteiO55135.
SwissPalmiO55135.

Proteomic databases

EPDiO55135.
PaxDbiO55135.
PRIDEiO55135.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029142; ENSMUSP00000029142; ENSMUSG00000027613.
GeneIDi16418.
KEGGimmu:16418.
UCSCiuc008nll.1. mouse.

Organism-specific databases

CTDi3692.
MGIiMGI:1196288. Eif6.

Phylogenomic databases

eggNOGiKOG3185. Eukaryota.
COG1976. LUCA.
HOGENOMiHOG000230605.
HOVERGENiHBG001300.
InParanoidiO55135.
KOiK03264.
OMAiNDWCAFT.
OrthoDBiEOG7V4B05.
PhylomeDBiO55135.
TreeFamiTF105396.

Miscellaneous databases

NextBioi289617.
PROiO55135.
SOURCEiSearch...

Gene expression databases

BgeeiO55135.
CleanExiMM_EIF6.
ExpressionAtlasiO55135. baseline and differential.
GenevisibleiO55135. MM.

Family and domain databases

HAMAPiMF_00032. eIF_6.
InterProiIPR002769. eIF6.
[Graphical view]
PANTHERiPTHR10784. PTHR10784. 1 hit.
PfamiPF01912. eIF-6. 1 hit.
[Graphical view]
PIRSFiPIRSF006413. IF-6. 1 hit.
SMARTiSM00654. eIF6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00323. eIF-6. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of murine translation initiation factor 6 and functional analysis of the homologous sequence YPR016c in Saccharomyces cerevisiae."
    Wood L.C., Ashby M.N., Grunfeld C., Feingold K.R.
    J. Biol. Chem. 274:11653-11659(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon and Kidney.
  3. "Isolation of a novel beta4 integrin-binding protein (p27(BBP)) highly expressed in epithelial cells."
    Biffo S., Sanvito F., Costa S., Preve L., Pignatelli R., Spinardi L., Marchisio P.C.
    J. Biol. Chem. 272:30314-30321(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 80-163.
    Strain: BALB/cJ.
  4. "The human ITGB4BP gene is constitutively expressed in vitro, but highly modulated in vivo."
    Donadini A., Giodini A., Sanvito F., Marchisio P.C., Biffo S.
    Gene 266:35-43(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "Eukaryotic initiation factor 6 is rate-limiting in translation, growth and transformation."
    Gandin V., Miluzio A., Barbieri A.M., Beugnet A., Kiyokawa H., Marchisio P.C., Biffo S.
    Nature 455:684-688(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  7. Cited for: PHOSPHORYLATION AT THR-165; SER-166; SER-174 AND SER-175, IDENTIFICATION AT THE 60S RIBOSOME SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiIF6_MOUSE
AccessioniPrimary (citable) accession number: O55135
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: April 13, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.