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Protein

Eukaryotic translation initiation factor 6

Gene

Eif6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR (PubMed:26383020). Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis (PubMed:26391622). Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (By similarity). Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth (PubMed:21665150).UniRule annotation4 Publications

GO - Molecular functioni

GO - Biological processi

  • assembly of large subunit precursor of preribosome Source: GO_Central
  • gene silencing by miRNA Source: UniProtKB
  • maturation of 5.8S rRNA Source: GO_Central
  • maturation of LSU-rRNA Source: GO_Central
  • mature ribosome assembly Source: UniProtKB
  • miRNA mediated inhibition of translation Source: UniProtKB
  • positive regulation of translation Source: UniProtKB
  • regulation of fatty acid biosynthetic process Source: UniProtKB
  • regulation of glycolytic process Source: UniProtKB
  • regulation of megakaryocyte differentiation Source: UniProtKB
  • regulation of reactive oxygen species metabolic process Source: UniProtKB
  • response to insulin Source: UniProtKB
  • ribosomal subunit export from nucleus Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Ribosome biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 6UniRule annotation
Short name:
eIF-6UniRule annotation
Alternative name(s):
B4 integrin interactor
CAB
p27(BBP)
Gene namesi
Name:Eif6
Synonyms:Itgb4bp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1196288. Eif6.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: GO_Central
  • extracellular exosome Source: MGI
  • intermediate filament Source: MGI
  • lamin filament Source: MGI
  • nucleolus Source: GO_Central
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • preribosome, large subunit precursor Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality. Embryos die at preimplantation stage.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi235 – 2351S → A: Abolishes insulin-stimulated translation, abrogates tumorigenesis. 3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Eukaryotic translation initiation factor 6PRO_0000153735Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei113 – 1131PhosphotyrosineBy similarity
Modified residuei165 – 1651PhosphothreonineUniRule annotation1 Publication
Modified residuei166 – 1661PhosphoserineUniRule annotation1 Publication
Modified residuei174 – 1741Phosphoserine; by CK1UniRule annotation1 Publication
Modified residuei175 – 1751Phosphoserine; by CK1UniRule annotation1 Publication
Modified residuei235 – 2351Phosphoserine; by PKCUniRule annotation1 Publication
Modified residuei239 – 2391PhosphoserineUniRule annotationBy similarity
Modified residuei243 – 2431PhosphoserineUniRule annotationBy similarity

Post-translational modificationi

Phosphorylation at Ser-174 and Ser-175 by CSNK1D/CK1 promotes nuclear export.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO55135.
PaxDbiO55135.
PeptideAtlasiO55135.
PRIDEiO55135.

PTM databases

iPTMnetiO55135.
PhosphoSiteiO55135.
SwissPalmiO55135.

Expressioni

Tissue specificityi

Detected in bladder, duodenum, liver, esophagus, pancreas, adipose tissue, megakaryocytes and testis with lower levels in muscle (at protein level).3 Publications

Gene expression databases

BgeeiENSMUSG00000027613.
CleanExiMM_EIF6.
ExpressionAtlasiO55135. baseline and differential.
GenevisibleiO55135. MM.

Interactioni

Subunit structurei

Monomer. Associates with the 60S ribosomal subunit. Interacts with RACK1. Interacts with DICER1, AGO2, TARBP2, MOV10 and RPL7A; they form a large RNA-induced silencing complex (RISC).UniRule annotation

Protein-protein interaction databases

BioGridi200831. 4 interactions.
IntActiO55135. 2 interactions.
STRINGi10090.ENSMUSP00000029142.

Structurei

3D structure databases

ProteinModelPortaliO55135.
SMRiO55135. Positions 1-224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-6 family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3185. Eukaryota.
COG1976. LUCA.
HOGENOMiHOG000230605.
HOVERGENiHBG001300.
InParanoidiO55135.
KOiK03264.
OMAiNDWCAFT.
OrthoDBiEOG091G0HYF.
PhylomeDBiO55135.
TreeFamiTF105396.

Family and domain databases

CDDicd00527. IF6. 1 hit.
HAMAPiMF_00032. eIF_6. 1 hit.
InterProiIPR002769. eIF6.
[Graphical view]
PANTHERiPTHR10784. PTHR10784. 1 hit.
PfamiPF01912. eIF-6. 1 hit.
[Graphical view]
PIRSFiPIRSF006413. IF-6. 1 hit.
SMARTiSM00654. eIF6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00323. eIF-6. 1 hit.

Sequencei

Sequence statusi: Complete.

O55135-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVRASFENN CEVGCFAKLT NAYCLVAIGG SENFYSVFEG ELSDAIPVVH
60 70 80 90 100
ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLPD SVQIRRVEER
110 120 130 140 150
LSALGNVTTC NDYVALVHPD LDRETEEILA DVLKVEVFRQ TVADQVLVGS
160 170 180 190 200
YCVFSNQGGL VHPKTSIEDQ DELSSLLQVP LVAGTVNRGS EVIAAGMVVN
210 220 230 240
DWCAFCGLDT TSTELSVVES VFKLNEAKPS TIATSMRDSL IDSLT
Length:245
Mass (Da):26,511
Last modified:May 30, 2000 - v2
Checksum:i35BF0F4C17D5E045
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047046 mRNA. Translation: AAD28078.1.
BC015274 mRNA. Translation: AAH15274.1.
BC024442 mRNA. Translation: AAH24442.1.
Y11460 mRNA. Translation: CAA72246.1.
CCDSiCCDS16956.1.
RefSeqiNP_034709.1. NM_010579.2.
UniGeneiMm.271674.

Genome annotation databases

EnsembliENSMUST00000029142; ENSMUSP00000029142; ENSMUSG00000027613.
GeneIDi16418.
KEGGimmu:16418.
UCSCiuc008nll.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047046 mRNA. Translation: AAD28078.1.
BC015274 mRNA. Translation: AAH15274.1.
BC024442 mRNA. Translation: AAH24442.1.
Y11460 mRNA. Translation: CAA72246.1.
CCDSiCCDS16956.1.
RefSeqiNP_034709.1. NM_010579.2.
UniGeneiMm.271674.

3D structure databases

ProteinModelPortaliO55135.
SMRiO55135. Positions 1-224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200831. 4 interactions.
IntActiO55135. 2 interactions.
STRINGi10090.ENSMUSP00000029142.

PTM databases

iPTMnetiO55135.
PhosphoSiteiO55135.
SwissPalmiO55135.

Proteomic databases

EPDiO55135.
PaxDbiO55135.
PeptideAtlasiO55135.
PRIDEiO55135.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029142; ENSMUSP00000029142; ENSMUSG00000027613.
GeneIDi16418.
KEGGimmu:16418.
UCSCiuc008nll.1. mouse.

Organism-specific databases

CTDi3692.
MGIiMGI:1196288. Eif6.

Phylogenomic databases

eggNOGiKOG3185. Eukaryota.
COG1976. LUCA.
HOGENOMiHOG000230605.
HOVERGENiHBG001300.
InParanoidiO55135.
KOiK03264.
OMAiNDWCAFT.
OrthoDBiEOG091G0HYF.
PhylomeDBiO55135.
TreeFamiTF105396.

Miscellaneous databases

PROiO55135.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027613.
CleanExiMM_EIF6.
ExpressionAtlasiO55135. baseline and differential.
GenevisibleiO55135. MM.

Family and domain databases

CDDicd00527. IF6. 1 hit.
HAMAPiMF_00032. eIF_6. 1 hit.
InterProiIPR002769. eIF6.
[Graphical view]
PANTHERiPTHR10784. PTHR10784. 1 hit.
PfamiPF01912. eIF-6. 1 hit.
[Graphical view]
PIRSFiPIRSF006413. IF-6. 1 hit.
SMARTiSM00654. eIF6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00323. eIF-6. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIF6_MOUSE
AccessioniPrimary (citable) accession number: O55135
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: September 7, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.