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Protein

Histone deacetylase complex subunit SAP18

Gene

Sap18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP and PSAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. The ASAP complex can inhibit mRNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits the formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-427413. NoRC negatively regulates rRNA expression.
R-MMU-573389. NoRC negatively regulates rRNA expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone deacetylase complex subunit SAP18
Alternative name(s):
18 kDa Sin3-associated polypeptide
Sin3-associated polypeptide p18
Gene namesi
Name:Sap18
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1277978. Sap18.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity
  • Nucleus speckle By similarity

  • Note: Shuttles between the nucleus and the cytoplasm (By similarity). Colocalizes with ACIN1 and SRSF2 in nuclear speckles (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi26C → R: Impairs interactions with RNPS1, ACIN1 and PNN; reduces ASAP and PSAP complex assemblies. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002209762 – 153Histone deacetylase complex subunit SAP18Add BLAST152

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki13Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiO55128.
MaxQBiO55128.
PaxDbiO55128.
PeptideAtlasiO55128.
PRIDEiO55128.

PTM databases

iPTMnetiO55128.
PhosphoSitePlusiO55128.

Expressioni

Tissue specificityi

Expressed in all tissues tested; highest levels in the brain, kidney and muscle; lowest levels in lung spleen, liver, intestine and testis, and moderate levels in salivary gland and heart.1 Publication

Gene expression databases

BgeeiENSMUSG00000021963.
CleanExiMM_SAP18.

Interactioni

Subunit structurei

Found in a mRNA splicing-dependent exon junction complex (EJC). Component of the heterotrimeric ASAP (apoptosis- and splicing-associated protein) and PSAP complexes consisting of RNPS1, SAP18 and either ACIN1 or PNN, respectively; the ASAP and PSAP complexes probably are formed mutually exclusive. For the ASAP complex, the association of SAP18 seems to require a preformed RNPS1:ACIN1 complex. Forms a complex with SIN3A and HDAC1. Interacts with SUFU.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SufuQ9Z0P76EBI-3508332,EBI-3508336

Protein-protein interaction databases

BioGridi203070. 8 interactors.
DIPiDIP-59924N.
IntActiO55128. 6 interactors.
MINTiMINT-4135244.
STRINGi10090.ENSMUSP00000120071.

Structurei

Secondary structure

1153
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Helixi22 – 24Combined sources3
Beta strandi28 – 39Combined sources12
Helixi43 – 46Combined sources4
Helixi47 – 49Combined sources3
Beta strandi56 – 60Combined sources5
Helixi66 – 76Combined sources11
Helixi78 – 81Combined sources4
Beta strandi86 – 94Combined sources9
Beta strandi96 – 110Combined sources15
Turni116 – 119Combined sources4
Helixi123 – 125Combined sources3
Beta strandi132 – 138Combined sources7
Helixi140 – 142Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4A6QX-ray1.50A6-143[»]
4A8XX-ray1.90C14-143[»]
4A90X-ray1.90A/B1-143[»]
ProteinModelPortaliO55128.
SMRiO55128.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 153Involved in splicing regulation activityBy similarityAdd BLAST61

Sequence similaritiesi

Belongs to the SAP18 family.Curated

Phylogenomic databases

eggNOGiKOG3391. Eukaryota.
ENOG4111FBB. LUCA.
HOGENOMiHOG000238745.
HOVERGENiHBG003465.
InParanoidiO55128.
KOiK14324.
PhylomeDBiO55128.

Family and domain databases

InterProiIPR017250. Hist_deAcase_cplx_SAP18.
IPR010516. SAP18.
[Graphical view]
PANTHERiPTHR13082. PTHR13082. 1 hit.
PfamiPF06487. SAP18. 1 hit.
[Graphical view]
PIRSFiPIRSF037637. HDAC_SAP18. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55128-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVESRVTQE EIKKEPEKPI DREKTCPLLL RVFTTNNGRH HRMDEFSRGN
60 70 80 90 100
VPSSELQIYT WMDATLKELT SLVKEVYPEA RKKGTHFNFA IVFMDLKRPG
110 120 130 140 150
YRVKEIGSTM SGRKGTDDSM TLQSQKFQIG DYLDIAITPP NRAPPSSGRM

RPY
Length:153
Mass (Da):17,595
Last modified:June 1, 1998 - v1
Checksum:i68FE63E6EAD04E6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97062 mRNA. Translation: CAB09797.1.
AK005189 mRNA. Translation: BAB23871.1.
AK006647 mRNA. Translation: BAB24687.1.
AK085992 mRNA. Translation: BAC39588.1.
BC006625 mRNA. Translation: AAH06625.1.
RefSeqiNP_033145.2. NM_009119.3.
UniGeneiMm.30173.
Mm.344671.

Genome annotation databases

GeneIDi20220.
KEGGimmu:20220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97062 mRNA. Translation: CAB09797.1.
AK005189 mRNA. Translation: BAB23871.1.
AK006647 mRNA. Translation: BAB24687.1.
AK085992 mRNA. Translation: BAC39588.1.
BC006625 mRNA. Translation: AAH06625.1.
RefSeqiNP_033145.2. NM_009119.3.
UniGeneiMm.30173.
Mm.344671.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4A6QX-ray1.50A6-143[»]
4A8XX-ray1.90C14-143[»]
4A90X-ray1.90A/B1-143[»]
ProteinModelPortaliO55128.
SMRiO55128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203070. 8 interactors.
DIPiDIP-59924N.
IntActiO55128. 6 interactors.
MINTiMINT-4135244.
STRINGi10090.ENSMUSP00000120071.

PTM databases

iPTMnetiO55128.
PhosphoSitePlusiO55128.

Proteomic databases

EPDiO55128.
MaxQBiO55128.
PaxDbiO55128.
PeptideAtlasiO55128.
PRIDEiO55128.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20220.
KEGGimmu:20220.

Organism-specific databases

CTDi10284.
MGIiMGI:1277978. Sap18.

Phylogenomic databases

eggNOGiKOG3391. Eukaryota.
ENOG4111FBB. LUCA.
HOGENOMiHOG000238745.
HOVERGENiHBG003465.
InParanoidiO55128.
KOiK14324.
PhylomeDBiO55128.

Enzyme and pathway databases

ReactomeiR-MMU-427413. NoRC negatively regulates rRNA expression.
R-MMU-573389. NoRC negatively regulates rRNA expression.

Miscellaneous databases

PROiO55128.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021963.
CleanExiMM_SAP18.

Family and domain databases

InterProiIPR017250. Hist_deAcase_cplx_SAP18.
IPR010516. SAP18.
[Graphical view]
PANTHERiPTHR13082. PTHR13082. 1 hit.
PfamiPF06487. SAP18. 1 hit.
[Graphical view]
PIRSFiPIRSF037637. HDAC_SAP18. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSAP18_MOUSE
AccessioniPrimary (citable) accession number: O55128
Secondary accession number(s): Q545L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.