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O55112 (AFF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AF4/FMR2 family member 2
Alternative name(s):
FMR2P
Fragile X mental retardation protein 2 homolog
Short name=Protein FMR-2
Protein Ox19
Gene names
Name:Aff2
Synonyms:Fmr2, Ox19
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1272 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

RNA-binding protein. Might be involved in alternative splicing regulation through an interaction with G-quartet RNA structure. Ref.3

Subcellular location

Nucleus speckle. Note: When splicing or transcription are inhibited, accumulates in large, rounded speckles and in the nucleolus By similarity. Ref.3

Tissue specificity

Highly expressed in the hippocampus, the piriform cortex, Purkinje cells and the cingulate gyrus.

Developmental stage

Expressed before day 7 in the embryo and reached its highest levels at 10.5-11.5 days. In the embryo at day 11, expression is more specific in the roof of the hind brain and the lateral ventricle of the brain.

Sequence similarities

Belongs to the AF4 family.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentNucleus
   LigandRNA-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA splicing

Inferred from electronic annotation. Source: UniProtKB-KW

learning or memory

Inferred from mutant phenotype PubMed 11923441. Source: MGI

mRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of RNA splicing

Inferred from direct assay Ref.3. Source: UniProtKB

   Cellular_componentnuclear speck

Inferred from direct assay Ref.3. Source: UniProtKB

   Molecular_functionG-quadruplex RNA binding

Inferred from direct assay Ref.3. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12721272AF4/FMR2 family member 2
PRO_0000215913

Amino acid modifications

Modified residue3951Phosphoserine By similarity
Modified residue3961Phosphoserine By similarity
Modified residue4821Phosphothreonine By similarity

Experimental info

Sequence conflict4321P → S in CAA04821. Ref.1
Sequence conflict5441A → S in CAA04821. Ref.1
Sequence conflict9411V → A in CAA04821. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O55112 [UniParc].

Last modified February 9, 2010. Version 2.
Checksum: 1CE41F1AEFE9C2B4

FASTA1,272140,181
        10         20         30         40         50         60 
MDLFDFFRDW DLEQQCHYEQ DRSALKKREW ERRNQEVQQE EDLFSSGFDL FGEPYKVAEY 

        70         80         90        100        110        120 
TNKGDALANR VQNTLGSYDE MKDLLSNHSS QNHLVGIPKN SAPQTPISKS EASFYPEQKN 

       130        140        150        160        170        180 
RMIPSHQETT HSSTPMPPPS VVILNSTLIH SNRKSKSEWP RDSHNTSPAQ ASQTSSQPNK 

       190        200        210        220        230        240 
MQTSTQDPPQ TRLEDFFVYP AEQPQIGTVE KSNPSSKEEN NPNSGGEDTF KEIFQSNSPE 

       250        260        270        280        290        300 
ESEFTVQAPG SPLVASSLLA PSSGLSVPTF PPGLYCKTSM GQQKPTAYVR PMDGQDQATD 

       310        320        330        340        350        360 
ISPTLKPSIE FENSFGNLSF GSLLDGKPSA VSSKTKLPKF TILQTSEVSL TSDPSCVEEI 

       370        380        390        400        410        420 
LRESQHLTPG FTLQKWSDPS SRASTKMLED DLKLSSDEDD LEPVKTLTTQ CTANELYQAV 

       430        440        450        460        470        480 
EKAKPKNNPV NPLLATPQST PATQTNVGSG SSSESESSSE SDSDTESSTT DSESNEAPRV 

       490        500        510        520        530        540 
ATPEPEPPST NKWQLDKWLN KVTSQNKSFI CGQNETPTET ISLPPPIIQP VEVQVKVKPN 

       550        560        570        580        590        600 
PSQAVAVPKE RPLLSLIREK ARPRPTQKTP ETKALKHKLS TSVDTVSQRT IGKKQPKKVE 

       610        620        630        640        650        660 
KNTSFEEFTW PKPNITNSTP KEKGSVELPD PPRSRNKATA HKPVPRKEPR PHVPLATEKK 

       670        680        690        700        710        720 
KYRGPGKIVP KSREFIETDS STSDSNTDQE ETLQIKVLPP CITSKSKETS NASLTLSTLT 

       730        740        750        760        770        780 
NGNSNNLSTS NEETAFSPPP AMQTELLSPL RDHENPKNLW VKIDLDLLSR VPGQNSVPVT 

       790        800        810        820        830        840 
PAKTDYKETA SKPKRQTAAT AVEKPAPKGK RKHKPAETAE KIPEKKQRLE DNTTICLLPP 

       850        860        870        880        890        900 
CISPAPPHKP PSTRENSSRR ANRKKEEKLF PPALSPLAED PPRRRNVSGN NGHFGQDKNI 

       910        920        930        940        950        960 
SMAGQITSSK PKRSEGKFCA TFKGISINEG DAPKKAASAT VTVANMALAT ATATATVPAI 

       970        980        990       1000       1010       1020 
VTATVTATAT TTATATTTTT TTTISSITPT ITSGLMDSSH LEMTSWAALP LLSSSSANVR 

      1030       1040       1050       1060       1070       1080 
RPKLTFDDSV HNADFYMQEA KKLKHKADAL FEKFGKAVNY ADAALSFTEC GNAMERDPLE 

      1090       1100       1110       1120       1130       1140 
AKSPYTMYSE TVELLRYAMR LKNFASPLAS DGDKKLAVLC YRCLSLLYLR MFKLKKDHAM 

      1150       1160       1170       1180       1190       1200 
KYSRSLMEYF KQNASKVTQI PSPWVGNGKN TPSPVSLNNV SPINSVGNCN NGPVTIPQRI 

      1210       1220       1230       1240       1250       1260 
HHMAASHVNI TSNVLRGYEH WDMADKLTRD NKEFFGDLDT LMGPLTQHSS MTNLVRYVRQ 

      1270 
GLCWLRIDAH LL 

« Hide

References

« Hide 'large scale' references
[1]"Expression of the murine homologue of FMR2 in mouse brain and during development."
Chakrabarti L., Bristulf J., Foss G.S., Davies K.E.
Hum. Mol. Genet. 7:441-448(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"FRAXE-associated mental retardation protein (FMR2) is an RNA-binding protein with high affinity for G-quartet RNA forming structure."
Bensaid M., Melko M., Bechara E.G., Davidovic L., Berretta A., Catania M.V., Gecz J., Lalli E., Bardoni B.
Nucleic Acids Res. 37:1269-1279(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ001549 mRNA. Translation: CAA04821.1.
AL663113 expand/collapse EMBL AC list , AL672120, AL808131, BX294655 Genomic DNA. Translation: CAM17463.1.
BX294655 expand/collapse EMBL AC list , AL663113, AL672120, AL808131 Genomic DNA. Translation: CAM20870.1.
AL808131 expand/collapse EMBL AC list , AL663113, AL672120, BX294655 Genomic DNA. Translation: CAM22352.1.
CCDSCCDS30173.1.
PIRT30248.
RefSeqNP_032058.2. NM_008032.3.
UniGeneMm.466576.

3D structure databases

ProteinModelPortalO55112.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199717. 2 interactions.
STRING10090.ENSMUSP00000033532.

PTM databases

PhosphoSiteO55112.

Proteomic databases

PRIDEO55112.

Protocols and materials databases

DNASU14266.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033532; ENSMUSP00000033532; ENSMUSG00000031189.
GeneID14266.
KEGGmmu:14266.
UCSCuc009tjb.1. mouse.

Organism-specific databases

CTD2334.
MGIMGI:1202294. Aff2.

Phylogenomic databases

eggNOGNOG69292.
GeneTreeENSGT00530000063217.
HOGENOMHOG000246991.
HOVERGENHBG004189.
InParanoidB1ATW0.
KOK15194.
OMADEFTWPK.
OrthoDBEOG767390.
PhylomeDBO55112.
TreeFamTF326216.

Gene expression databases

BgeeO55112.
CleanExMM_AFF2.
GenevestigatorO55112.

Family and domain databases

InterProIPR007797. TF_AF4/FMR2.
[Graphical view]
PANTHERPTHR10528. PTHR10528. 1 hit.
PfamPF05110. AF-4. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio285623.
PROO55112.
SOURCESearch...

Entry information

Entry nameAFF2_MOUSE
AccessionPrimary (citable) accession number: O55112
Secondary accession number(s): B1ATW0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 9, 2010
Last modified: July 9, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot