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O55099

- AURKB_RAT

UniProt

O55099 - AURKB_RAT

Protein

Aurora kinase B

Gene

Aurkb

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). AURKB is also required for kinetochore localization of BUB1 and SGOL1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.

    Enzyme regulationi

    Activity is greatly increased when AURKB is within the CPC complex. In particular, AURKB-phosphorylated INCENP acts as an activator of AURKB By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091ATP
    Active sitei203 – 2031Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi86 – 949ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. histone serine kinase activity Source: UniProtKB
    3. metal ion binding Source: UniProtKB-KW
    4. protein kinase activity Source: RGD
    5. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. abscission Source: UniProtKB
    2. aging Source: RGD
    3. cell proliferation Source: RGD
    4. cellular response to UV Source: UniProtKB
    5. cleavage furrow formation Source: UniProtKB
    6. cytokinesis checkpoint Source: UniProtKB
    7. histone H3-S28 phosphorylation Source: UniProtKB
    8. negative regulation of B cell apoptotic process Source: UniProtKB
    9. negative regulation of cytokinesis Source: UniProtKB
    10. negative regulation of protein binding Source: Ensembl
    11. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    12. positive regulation of cytokinesis Source: UniProtKB
    13. protein localization to kinetochore Source: UniProtKB
    14. protein phosphorylation Source: UniProtKB
    15. spindle checkpoint Source: InterPro
    16. spindle midzone assembly involved in mitosis Source: UniProtKB
    17. spindle stabilization Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_194781. Separation of Sister Chromatids.
    REACT_198611. Resolution of Sister Chromatid Cohesion.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aurora kinase B (EC:2.7.11.1)
    Alternative name(s):
    Aurora 1
    Aurora- and IPL1-like midbody-associated protein 1
    Aurora/IPL1-related kinase 2
    Short name:
    ARK-2
    Short name:
    Aurora-related kinase 2
    STK-1
    Serine/threonine-protein kinase 12
    Serine/threonine-protein kinase 5
    Serine/threonine-protein kinase aurora-B
    Gene namesi
    Name:Aurkb
    Synonyms:Aik2, Aim1, Airk2, Ark2, Stk1, Stk12, Stk5
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi621625. Aurkb.

    Subcellular locationi

    Nucleus By similarity. Chromosome By similarity. Chromosomecentromere By similarity. Cytoplasmcytoskeletonspindle By similarity. Midbody By similarity
    Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body By similarity. Proper localization of the active, Thr-235-phosphorylated form during metaphase may be dependent upon interaction with SPDYC. Interacts with p53/TP53. Interacts (via the middle kinase domain) with NOC2L (via the N- and C-terminus domains). Colocalized with SIRT2 during cytokinesis with the midbody By similarity.By similarity

    GO - Cellular componenti

    1. chromocenter Source: Ensembl
    2. chromosome passenger complex Source: Ensembl
    3. condensed nuclear chromosome, centromeric region Source: Ensembl
    4. cytoplasm Source: UniProtKB-KW
    5. midbody Source: RGD
    6. nucleus Source: UniProtKB
    7. spindle Source: RGD

    Keywords - Cellular componenti

    Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi109 – 1091K → R: Loss of kinase activity, disruption of cleavage furrow formation, failure of cytokinesis leading to cell polyploidy death. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 343343Aurora kinase BPRO_0000085659Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei235 – 2351Phosphothreonine; by autocatalysisBy similarity

    Post-translational modificationi

    The phosphorylation of Thr-235 requires the binding to INCENP and occurs by means of an autophosphorylation mechanism. Thr-235 phosphorylation is indispensable for the AURKB kinase activity By similarity.By similarity
    Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiO55099.

    PTM databases

    PhosphoSiteiO55099.

    Expressioni

    Tissue specificityi

    High level expression seen in the testis. It is also expressed in the spleen, lung and heart. Expressed in the G2/M phase of the cell cycle.1 Publication

    Gene expression databases

    GenevestigatoriO55099.

    Interactioni

    Subunit structurei

    Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC By similarity. Associates with RACGAP1 during M phase. Interacts with CDCA1, EVI5, JTB, NDC80, PSMA3, SEPT1, SIRT2 and TACC1 By similarity. Interacts with SPDYC; this interaction may be required for proper localization of active, Thr-235-phosphorylated AURKB form during prometaphase and metaphase By similarity. Interacts with TTC28. Interacts with RNF2/RING1B By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000008492.

    Structurei

    3D structure databases

    ProteinModelPortaliO55099.
    SMRiO55099. Positions 75-340.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini80 – 330251Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000074590.
    HOGENOMiHOG000233016.
    HOVERGENiHBG108519.
    InParanoidiO55099.
    KOiK11479.
    OMAiHPWVRAN.
    OrthoDBiEOG74FF1F.
    PhylomeDBiO55099.
    TreeFamiTF351439.

    Family and domain databases

    InterProiIPR028772. AURKB.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24350:SF4. PTHR24350:SF4. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O55099-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQKENVYPW PYGSKTSQSG LNTLPQRVLR KEPAVTPAQA LMNRSNSQST    50
    AVPGQKLTEN KGATALQGSQ SRQPFTIDNF EIGRPLGKGK FGNVYLAREK 100
    KSRFIVALKI LFKSQIEKEG VEHQLRREIE IQAHLKHPNI LQLYNYFYDQ 150
    QRIYLILEYA PRGELYKELQ KSGTFDEQRT ATIMEELSDA LMYCHKKKVI 200
    HRDIKPENLL LGLQGELKIA DFGWSVHAPS LRRKTMCGTL DYLPPEMIEG 250
    RMHNEMVDLW CIGVLCYELM VGNPPFESPS HSETYRRIVK VDLKFPSSMP 300
    LGAKDLISKL LKHNPSQRLP LEQVSAHPWV RANSRRVLPP SAL 343
    Length:343
    Mass (Da):39,234
    Last modified:June 1, 1998 - v1
    Checksum:i27B740D20E287598
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D89731 mRNA. Translation: BAA23794.1.
    BC097297 mRNA. Translation: AAH97297.1.
    RefSeqiNP_446201.1. NM_053749.1.
    XP_006246631.1. XM_006246569.1.
    UniGeneiRn.10865.

    Genome annotation databases

    EnsembliENSRNOT00000008492; ENSRNOP00000008492; ENSRNOG00000005659.
    GeneIDi114592.
    KEGGirno:114592.
    UCSCiRGD:621625. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D89731 mRNA. Translation: BAA23794.1 .
    BC097297 mRNA. Translation: AAH97297.1 .
    RefSeqi NP_446201.1. NM_053749.1.
    XP_006246631.1. XM_006246569.1.
    UniGenei Rn.10865.

    3D structure databases

    ProteinModelPortali O55099.
    SMRi O55099. Positions 75-340.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000008492.

    PTM databases

    PhosphoSitei O55099.

    Proteomic databases

    PRIDEi O55099.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000008492 ; ENSRNOP00000008492 ; ENSRNOG00000005659 .
    GeneIDi 114592.
    KEGGi rno:114592.
    UCSCi RGD:621625. rat.

    Organism-specific databases

    CTDi 9212.
    RGDi 621625. Aurkb.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000074590.
    HOGENOMi HOG000233016.
    HOVERGENi HBG108519.
    InParanoidi O55099.
    KOi K11479.
    OMAi HPWVRAN.
    OrthoDBi EOG74FF1F.
    PhylomeDBi O55099.
    TreeFami TF351439.

    Enzyme and pathway databases

    Reactomei REACT_194781. Separation of Sister Chromatids.
    REACT_198611. Resolution of Sister Chromatid Cohesion.

    Miscellaneous databases

    NextBioi 618749.
    PROi O55099.

    Gene expression databases

    Genevestigatori O55099.

    Family and domain databases

    InterProi IPR028772. AURKB.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24350:SF4. PTHR24350:SF4. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "AIM-1: a mammalian midbody-associated protein required for cytokinesis."
      Terada Y., Tatsuka M., Suzuki F., Yasuda Y., Fujita S., Otsu M.
      EMBO J. 17:667-676(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-109.
      Tissue: Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiAURKB_RAT
    AccessioniPrimary (citable) accession number: O55099
    Secondary accession number(s): Q4V8N1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 17, 2003
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3