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O55099

- AURKB_RAT

UniProt

O55099 - AURKB_RAT

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Protein

Aurora kinase B

Gene

Aurkb

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). AURKB is also required for kinetochore localization of BUB1 and SGOL1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes By similarity.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.

Enzyme regulationi

Activity is greatly increased when AURKB is within the CPC complex. In particular, AURKB-phosphorylated INCENP acts as an activator of AURKB By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091ATP
Active sitei203 – 2031Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 949ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. histone serine kinase activity Source: UniProtKB
  3. metal ion binding Source: UniProtKB-KW
  4. protein kinase activity Source: RGD
  5. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. abscission Source: UniProtKB
  2. aging Source: RGD
  3. cell proliferation Source: RGD
  4. cellular response to UV Source: UniProtKB
  5. cleavage furrow formation Source: UniProtKB
  6. cytokinesis checkpoint Source: UniProtKB
  7. histone H3-S28 phosphorylation Source: UniProtKB
  8. negative regulation of B cell apoptotic process Source: UniProtKB
  9. negative regulation of cytokinesis Source: UniProtKB
  10. negative regulation of protein binding Source: Ensembl
  11. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  12. positive regulation of cytokinesis Source: UniProtKB
  13. protein localization to kinetochore Source: UniProtKB
  14. protein phosphorylation Source: UniProtKB
  15. spindle checkpoint Source: InterPro
  16. spindle midzone assembly involved in mitosis Source: UniProtKB
  17. spindle stabilization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_194781. Separation of Sister Chromatids.
REACT_198611. Resolution of Sister Chromatid Cohesion.

Names & Taxonomyi

Protein namesi
Recommended name:
Aurora kinase B (EC:2.7.11.1)
Alternative name(s):
Aurora 1
Aurora- and IPL1-like midbody-associated protein 1
Aurora/IPL1-related kinase 2
Short name:
ARK-2
Short name:
Aurora-related kinase 2
STK-1
Serine/threonine-protein kinase 12
Serine/threonine-protein kinase 5
Serine/threonine-protein kinase aurora-B
Gene namesi
Name:Aurkb
Synonyms:Aik2, Aim1, Airk2, Ark2, Stk1, Stk12, Stk5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi621625. Aurkb.

Subcellular locationi

Nucleus By similarity. Chromosome By similarity. Chromosomecentromere By similarity. Cytoplasmcytoskeletonspindle By similarity. Midbody By similarity
Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body By similarity. Proper localization of the active, Thr-235-phosphorylated form during metaphase may be dependent upon interaction with SPDYC. Interacts with p53/TP53. Interacts (via the middle kinase domain) with NOC2L (via the N- and C-terminus domains). Colocalized with SIRT2 during cytokinesis with the midbody By similarity.By similarity

GO - Cellular componenti

  1. chromocenter Source: Ensembl
  2. chromosome passenger complex Source: Ensembl
  3. condensed nuclear chromosome, centromeric region Source: Ensembl
  4. cytoplasm Source: UniProtKB-KW
  5. intercellular bridge Source: Ensembl
  6. midbody Source: RGD
  7. nucleus Source: UniProtKB
  8. spindle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi109 – 1091K → R: Loss of kinase activity, disruption of cleavage furrow formation, failure of cytokinesis leading to cell polyploidy death. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343Aurora kinase BPRO_0000085659Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei235 – 2351Phosphothreonine; by autocatalysisBy similarity

Post-translational modificationi

The phosphorylation of Thr-235 requires the binding to INCENP and occurs by means of an autophosphorylation mechanism. Thr-235 phosphorylation is indispensable for the AURKB kinase activity By similarity.By similarity
Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome By similarity.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiO55099.

PTM databases

PhosphoSiteiO55099.

Expressioni

Tissue specificityi

High level expression seen in the testis. It is also expressed in the spleen, lung and heart. Expressed in the G2/M phase of the cell cycle.1 Publication

Gene expression databases

GenevestigatoriO55099.

Interactioni

Subunit structurei

Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC By similarity. Associates with RACGAP1 during M phase. Interacts with CDCA1, EVI5, JTB, NDC80, PSMA3, SEPT1, SIRT2 and TACC1 By similarity. Interacts with SPDYC; this interaction may be required for proper localization of active, Thr-235-phosphorylated AURKB form during prometaphase and metaphase By similarity. Interacts with TTC28. Interacts with RNF2/RING1B By similarity.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008492.

Structurei

3D structure databases

ProteinModelPortaliO55099.
SMRiO55099. Positions 75-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 330251Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074590.
HOGENOMiHOG000233016.
HOVERGENiHBG108519.
InParanoidiO55099.
KOiK11479.
OMAiHPWVRAN.
OrthoDBiEOG74FF1F.
PhylomeDBiO55099.
TreeFamiTF351439.

Family and domain databases

InterProiIPR028772. AURKB.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24350:SF4. PTHR24350:SF4. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O55099-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAQKENVYPW PYGSKTSQSG LNTLPQRVLR KEPAVTPAQA LMNRSNSQST
60 70 80 90 100
AVPGQKLTEN KGATALQGSQ SRQPFTIDNF EIGRPLGKGK FGNVYLAREK
110 120 130 140 150
KSRFIVALKI LFKSQIEKEG VEHQLRREIE IQAHLKHPNI LQLYNYFYDQ
160 170 180 190 200
QRIYLILEYA PRGELYKELQ KSGTFDEQRT ATIMEELSDA LMYCHKKKVI
210 220 230 240 250
HRDIKPENLL LGLQGELKIA DFGWSVHAPS LRRKTMCGTL DYLPPEMIEG
260 270 280 290 300
RMHNEMVDLW CIGVLCYELM VGNPPFESPS HSETYRRIVK VDLKFPSSMP
310 320 330 340
LGAKDLISKL LKHNPSQRLP LEQVSAHPWV RANSRRVLPP SAL
Length:343
Mass (Da):39,234
Last modified:June 1, 1998 - v1
Checksum:i27B740D20E287598
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D89731 mRNA. Translation: BAA23794.1.
BC097297 mRNA. Translation: AAH97297.1.
RefSeqiNP_446201.1. NM_053749.1.
XP_006246631.1. XM_006246569.2.
UniGeneiRn.10865.

Genome annotation databases

EnsembliENSRNOT00000008492; ENSRNOP00000008492; ENSRNOG00000005659.
GeneIDi114592.
KEGGirno:114592.
UCSCiRGD:621625. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D89731 mRNA. Translation: BAA23794.1 .
BC097297 mRNA. Translation: AAH97297.1 .
RefSeqi NP_446201.1. NM_053749.1.
XP_006246631.1. XM_006246569.2.
UniGenei Rn.10865.

3D structure databases

ProteinModelPortali O55099.
SMRi O55099. Positions 75-340.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000008492.

PTM databases

PhosphoSitei O55099.

Proteomic databases

PRIDEi O55099.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000008492 ; ENSRNOP00000008492 ; ENSRNOG00000005659 .
GeneIDi 114592.
KEGGi rno:114592.
UCSCi RGD:621625. rat.

Organism-specific databases

CTDi 9212.
RGDi 621625. Aurkb.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074590.
HOGENOMi HOG000233016.
HOVERGENi HBG108519.
InParanoidi O55099.
KOi K11479.
OMAi HPWVRAN.
OrthoDBi EOG74FF1F.
PhylomeDBi O55099.
TreeFami TF351439.

Enzyme and pathway databases

Reactomei REACT_194781. Separation of Sister Chromatids.
REACT_198611. Resolution of Sister Chromatid Cohesion.

Miscellaneous databases

NextBioi 618749.
PROi O55099.

Gene expression databases

Genevestigatori O55099.

Family and domain databases

InterProi IPR028772. AURKB.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24350:SF4. PTHR24350:SF4. 1 hit.
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "AIM-1: a mammalian midbody-associated protein required for cytokinesis."
    Terada Y., Tatsuka M., Suzuki F., Yasuda Y., Fujita S., Otsu M.
    EMBO J. 17:667-676(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-109.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiAURKB_RAT
AccessioniPrimary (citable) accession number: O55099
Secondary accession number(s): Q4V8N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3