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Protein

Aurora kinase B

Gene

Aurkb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). AURKB is also required for kinetochore localization of BUB1 and SGOL1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Enzyme regulationi

Activity is greatly increased when AURKB is within the CPC complex. In particular, AURKB-phosphorylated INCENP acts as an activator of AURKB (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091ATP
Active sitei203 – 2031Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 949ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_279869. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_297906. Separation of Sister Chromatids.
REACT_316496. Resolution of Sister Chromatid Cohesion.
REACT_338831. Mitotic Prometaphase.
REACT_362301. RHO GTPases Activate Formins.

Names & Taxonomyi

Protein namesi
Recommended name:
Aurora kinase B (EC:2.7.11.1)
Alternative name(s):
Aurora 1
Aurora- and IPL1-like midbody-associated protein 1
Aurora/IPL1-related kinase 2
Short name:
ARK-2
Short name:
Aurora-related kinase 2
STK-1
Serine/threonine-protein kinase 12
Serine/threonine-protein kinase 5
Serine/threonine-protein kinase aurora-B
Gene namesi
Name:Aurkb
Synonyms:Aik2, Aim1, Airk2, Ark2, Stk1, Stk12, Stk5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi621625. Aurkb.

Subcellular locationi

  • Nucleus By similarity
  • Chromosome By similarity
  • Chromosomecentromere By similarity
  • Cytoplasmcytoskeletonspindle By similarity
  • Midbody By similarity

  • Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body (By similarity). Proper localization of the active, Thr-235-phosphorylated form during metaphase may be dependent upon interaction with SPDYC. Interacts with p53/TP53. Interacts (via the middle kinase domain) with NOC2L (via the N- and C-terminus domains). Colocalized with SIRT2 during cytokinesis with the midbody (By similarity).By similarity

GO - Cellular componenti

  • chromocenter Source: Ensembl
  • chromosome passenger complex Source: GO_Central
  • condensed nuclear chromosome, centromeric region Source: GO_Central
  • cytoplasm Source: UniProtKB-KW
  • midbody Source: RGD
  • nucleus Source: UniProtKB
  • spindle Source: RGD
  • spindle microtubule Source: GO_Central
  • spindle midzone Source: GO_Central
  • spindle pole centrosome Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi109 – 1091K → R: Loss of kinase activity, disruption of cleavage furrow formation, failure of cytokinesis leading to cell polyploidy death. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343Aurora kinase BPRO_0000085659Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei235 – 2351Phosphothreonine; by autocatalysisBy similarity

Post-translational modificationi

The phosphorylation of Thr-235 requires the binding to INCENP and occurs by means of an autophosphorylation mechanism. Thr-235 phosphorylation is indispensable for the AURKB kinase activity (By similarity).By similarity
Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiO55099.

PTM databases

PhosphoSiteiO55099.

Expressioni

Tissue specificityi

High level expression seen in the testis. It is also expressed in the spleen, lung and heart. Expressed in the G2/M phase of the cell cycle.1 Publication

Gene expression databases

GenevisibleiO55099. RN.

Interactioni

Subunit structurei

Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC (By similarity). Associates with RACGAP1 during M phase. Interacts with CDCA1, EVI5, JTB, NDC80, PSMA3, SEPT1, SIRT2 and TACC1 (By similarity). Interacts with SPDYC; this interaction may be required for proper localization of active, Thr-235-phosphorylated AURKB form during prometaphase and metaphase (By similarity). Interacts with TTC28. Interacts with RNF2/RING1B (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008492.

Structurei

3D structure databases

ProteinModelPortaliO55099.
SMRiO55099. Positions 75-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 330251Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120530.
HOGENOMiHOG000233016.
HOVERGENiHBG108519.
InParanoidiO55099.
KOiK11479.
OMAiHPWVRAN.
OrthoDBiEOG74FF1F.
PhylomeDBiO55099.
TreeFamiTF351439.

Family and domain databases

InterProiIPR030616. Aur.
IPR028772. AURKB.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24350. PTHR24350. 1 hit.
PTHR24350:SF4. PTHR24350:SF4. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O55099-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQKENVYPW PYGSKTSQSG LNTLPQRVLR KEPAVTPAQA LMNRSNSQST
60 70 80 90 100
AVPGQKLTEN KGATALQGSQ SRQPFTIDNF EIGRPLGKGK FGNVYLAREK
110 120 130 140 150
KSRFIVALKI LFKSQIEKEG VEHQLRREIE IQAHLKHPNI LQLYNYFYDQ
160 170 180 190 200
QRIYLILEYA PRGELYKELQ KSGTFDEQRT ATIMEELSDA LMYCHKKKVI
210 220 230 240 250
HRDIKPENLL LGLQGELKIA DFGWSVHAPS LRRKTMCGTL DYLPPEMIEG
260 270 280 290 300
RMHNEMVDLW CIGVLCYELM VGNPPFESPS HSETYRRIVK VDLKFPSSMP
310 320 330 340
LGAKDLISKL LKHNPSQRLP LEQVSAHPWV RANSRRVLPP SAL
Length:343
Mass (Da):39,234
Last modified:June 1, 1998 - v1
Checksum:i27B740D20E287598
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89731 mRNA. Translation: BAA23794.1.
BC097297 mRNA. Translation: AAH97297.1.
RefSeqiNP_446201.1. NM_053749.1.
XP_006246631.1. XM_006246569.2.
XP_008765979.1. XM_008767757.1.
XP_008765980.1. XM_008767758.1.
UniGeneiRn.10865.

Genome annotation databases

EnsembliENSRNOT00000008492; ENSRNOP00000008492; ENSRNOG00000005659.
GeneIDi114592.
KEGGirno:114592.
UCSCiRGD:621625. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89731 mRNA. Translation: BAA23794.1.
BC097297 mRNA. Translation: AAH97297.1.
RefSeqiNP_446201.1. NM_053749.1.
XP_006246631.1. XM_006246569.2.
XP_008765979.1. XM_008767757.1.
XP_008765980.1. XM_008767758.1.
UniGeneiRn.10865.

3D structure databases

ProteinModelPortaliO55099.
SMRiO55099. Positions 75-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008492.

PTM databases

PhosphoSiteiO55099.

Proteomic databases

PRIDEiO55099.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000008492; ENSRNOP00000008492; ENSRNOG00000005659.
GeneIDi114592.
KEGGirno:114592.
UCSCiRGD:621625. rat.

Organism-specific databases

CTDi9212.
RGDi621625. Aurkb.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120530.
HOGENOMiHOG000233016.
HOVERGENiHBG108519.
InParanoidiO55099.
KOiK11479.
OMAiHPWVRAN.
OrthoDBiEOG74FF1F.
PhylomeDBiO55099.
TreeFamiTF351439.

Enzyme and pathway databases

ReactomeiREACT_279869. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_297906. Separation of Sister Chromatids.
REACT_316496. Resolution of Sister Chromatid Cohesion.
REACT_338831. Mitotic Prometaphase.
REACT_362301. RHO GTPases Activate Formins.

Miscellaneous databases

NextBioi618749.
PROiO55099.

Gene expression databases

GenevisibleiO55099. RN.

Family and domain databases

InterProiIPR030616. Aur.
IPR028772. AURKB.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24350. PTHR24350. 1 hit.
PTHR24350:SF4. PTHR24350:SF4. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "AIM-1: a mammalian midbody-associated protein required for cytokinesis."
    Terada Y., Tatsuka M., Suzuki F., Yasuda Y., Fujita S., Otsu M.
    EMBO J. 17:667-676(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-109.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiAURKB_RAT
AccessioniPrimary (citable) accession number: O55099
Secondary accession number(s): Q4V8N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: June 1, 1998
Last modified: June 24, 2015
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.