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Protein

Serine/threonine-protein kinase 10

Gene

Stk10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in regulation of lymphocyte migration. Phosphorylates MSN, and possibly PLK1. Involved in regulation of lymphocyte migration by mediating phosphorylation of ERM proteins such as MSN. Acts as a negative regulator of MAP3K1/MEKK1. May also act as a cell cycle regulator by acting as a polo kinase kinase: mediates phosphorylation of PLK1 in vitro; however such data require additional evidences in vivo.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Inhibited by the pyrrole-indolinone inhibitor SU11274 (K00593): intercalates between the ATP-binding Lys-65 and alpha-C glutamate (Glu-81), resulting in a partial disordering of the lysine side chain. Also specifically inhibited by erlotinib. Slightly inhibited by gefitinib (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65ATPPROSITE-ProRule annotation1
Binding sitei111InhibitorBy similarity1
Binding sitei113InhibitorBy similarity1
Binding sitei117Inhibitor; via amide nitrogenBy similarity1
Active sitei157Proton acceptorPROSITE-ProRule annotation1
Binding sitei175InhibitorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi42 – 50ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • lymphocyte aggregation Source: UniProtKB
  • protein autophosphorylation Source: UniProtKB
  • regulation of lymphocyte migration Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 10 (EC:2.7.11.1)
Alternative name(s):
Lymphocyte-oriented kinase
Gene namesi
Name:Stk10
Synonyms:Lok
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1099439. Stk10.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice do not show any obvious abnormalities. Lymphocytes develop normally but activated lymphocytes show enhanced cell adhesion. Decreased phosphorylation of ERM proteins.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000866981 – 966Serine/threonine-protein kinase 10Add BLAST966

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphoserineBy similarity1
Modified residuei20PhosphoserineBy similarity1
Modified residuei185Phosphothreonine; by autocatalysisBy similarity1
Modified residuei191PhosphoserineCombined sources1
Modified residuei437PhosphoserineBy similarity1
Modified residuei449PhosphoserineCombined sources1
Modified residuei453PhosphoserineCombined sources1
Modified residuei484PhosphoserineBy similarity1
Modified residuei513PhosphoserineBy similarity1
Modified residuei548PhosphoserineBy similarity1
Modified residuei950PhosphothreonineCombined sources1

Post-translational modificationi

Autophosphorylates following homodimerization, leading to activation of the protein.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO55098.
MaxQBiO55098.
PaxDbiO55098.
PRIDEiO55098.

PTM databases

iPTMnetiO55098.
PhosphoSitePlusiO55098.

Expressioni

Tissue specificityi

Expressed predominantly in lymphoid organs such as spleen, thymus, and bone marrow.

Gene expression databases

BgeeiENSMUSG00000020272.
CleanExiMM_STK10.
GenevisibleiO55098. MM.

Interactioni

Subunit structurei

Homodimer; homodimerization is required for activation segment autophosphorylation.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiO55098. 3 interactors.
MINTiMINT-4135895.
STRINGi10090.ENSMUSP00000099885.

Structurei

3D structure databases

ProteinModelPortaliO55098.
SMRiO55098.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 294Protein kinasePROSITE-ProRule annotationAdd BLAST259

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni175 – 224Activation segmentBy similarityAdd BLAST50

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili588 – 936Sequence analysisAdd BLAST349

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi749 – 883Gln-richAdd BLAST135

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0579. Eukaryota.
ENOG410XPQN. LUCA.
GeneTreeiENSGT00810000125351.
HOVERGENiHBG052712.
InParanoidiO55098.
KOiK08837.
OMAiSEEAECP.
OrthoDBiEOG091G01Y1.
TreeFamiTF351445.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR022165. PKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF12474. PKK. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O55098-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFANFRRIL RLSTFEKRKS REYEHVRRDL DPNDVWEIVG ELGDGAFGKV
60 70 80 90 100
YKAKNKETGA LAAAKVIETK SEEELEDYIV EIEILATCDH PYIVKLLGAY
110 120 130 140 150
YYDGKLWIMI EFCPGGAVDA IMLELDRGLT EPQIQVVCRQ MLEALNFLHG
160 170 180 190 200
KRIIHRDLKA GNVLMTLEGD IRLADFGVSA KNLKTLQKRD SFIGTPYWMA
210 220 230 240 250
PEVVLCETMK DAPYDYKADI WSLGITLIEM AQIEPPHHEL NPMRVLLKIA
260 270 280 290 300
KSDPPTLLTP SKWSVEFRDF LKIALDKNPE TRPSAAQLLQ HPFVSRVTSN
310 320 330 340 350
KALRELVAEA KAEVMEEIED GREDGEEEDA VDAVPPLVNH TQDSANVTQP
360 370 380 390 400
SLDSNKLLQD SSTPLPPSQP QEPVSGSCSQ PSGDGPLQTT SPADGLSKND
410 420 430 440 450
NDLKVPVPLR KSRPLSMDAR IQMDEEKQIP DQDENPSPAA SKSQKANQSR
460 470 480 490 500
PNSSALETLG GEALTNGGLE LPSSVTPSHS KRASDCSNLS TSESMDYGTS
510 520 530 540 550
LSADLSLNKE TGSLSLKGSK LHNKTLKRTR RFVVDGVEVS ITTSKIISED
560 570 580 590 600
EKKDEEMRFL RRQELRELRL LQKEEHRNQT QLSSKHELQL EQMHKRFEQE
610 620 630 640 650
INAKKKFYDV ELENLERQQK QQVEKMEQDH SVRRKEEAKR IRLEQDRDYA
660 670 680 690 700
KFQEQLKQMK KEVKSEVEKL PRQQRKESMK QKMEEHSQKK QRLDRDFVAK
710 720 730 740 750
QKEDLELAMR KLTTENRREI CDKERDCLSK KQELLRDREA ALWEMEEHQL
760 770 780 790 800
QERHQLVKQQ LKDQYFLQRH DLLRKHEKER EQMQRYNQRM MEQLKVRQQQ
810 820 830 840 850
EKARLPKIQR SDGKTRMAMY KKSLHINGAG SASEQREKIK QFSQQEEKRQ
860 870 880 890 900
KAERLQQQQK HENQMRDMVA QCESNMSELQ QLQNEKCHLL VEHETQKLKA
910 920 930 940 950
LDESHNQSLK EWRDKLRPRK KALEEDLNQK KREQEMFFKL SEEAEPRPTT
960
PSKASNFFPY SSGDAS
Length:966
Mass (Da):111,906
Last modified:July 27, 2011 - v2
Checksum:i7A95ABF9D58C8699
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti375 – 377SGS → NGP in BAA24073 (PubMed:9278426).Curated3
Sequence conflicti814K → E in BAA24073 (PubMed:9278426).Curated1
Sequence conflicti863N → H in BAA24073 (PubMed:9278426).Curated1
Sequence conflicti888H → Y in BAA24073 (PubMed:9278426).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89728 mRNA. Translation: BAA24073.1.
AL669844 Genomic DNA. Translation: CAI25517.1.
CCDSiCCDS24529.1.
RefSeqiNP_033314.2. NM_009288.2.
UniGeneiMm.8235.

Genome annotation databases

EnsembliENSMUST00000102821; ENSMUSP00000099885; ENSMUSG00000020272.
GeneIDi20868.
KEGGimmu:20868.
UCSCiuc007ijt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89728 mRNA. Translation: BAA24073.1.
AL669844 Genomic DNA. Translation: CAI25517.1.
CCDSiCCDS24529.1.
RefSeqiNP_033314.2. NM_009288.2.
UniGeneiMm.8235.

3D structure databases

ProteinModelPortaliO55098.
SMRiO55098.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO55098. 3 interactors.
MINTiMINT-4135895.
STRINGi10090.ENSMUSP00000099885.

PTM databases

iPTMnetiO55098.
PhosphoSitePlusiO55098.

Proteomic databases

EPDiO55098.
MaxQBiO55098.
PaxDbiO55098.
PRIDEiO55098.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102821; ENSMUSP00000099885; ENSMUSG00000020272.
GeneIDi20868.
KEGGimmu:20868.
UCSCiuc007ijt.1. mouse.

Organism-specific databases

CTDi6793.
MGIiMGI:1099439. Stk10.

Phylogenomic databases

eggNOGiKOG0579. Eukaryota.
ENOG410XPQN. LUCA.
GeneTreeiENSGT00810000125351.
HOVERGENiHBG052712.
InParanoidiO55098.
KOiK08837.
OMAiSEEAECP.
OrthoDBiEOG091G01Y1.
TreeFamiTF351445.

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiStk10. mouse.
PROiO55098.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020272.
CleanExiMM_STK10.
GenevisibleiO55098. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR022165. PKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF12474. PKK. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTK10_MOUSE
AccessioniPrimary (citable) accession number: O55098
Secondary accession number(s): B1ATW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.