Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O55098 (STK10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase 10

EC=2.7.11.1
Alternative name(s):
Lymphocyte-oriented kinase
Gene names
Name:Stk10
Synonyms:Lok
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length966 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine/threonine-protein kinase involved in regulation of lymphocyte migration. Phosphorylates MSN, and possibly PLK1. Involved in regulation of lymphocyte migration by mediating phosphorylation of ERM proteins such as MSN. Acts as a negative regulator of MAP3K1/MEKK1. May also act as a cell cycle regulator by acting as a polo kinase kinase: mediates phosphorylation of PLK1 in vitro; however such data require additional evidences in vivo. Ref.6

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Inhibited by the pyrrole-indolinone inhibitor SU11274 (K00593): intercalates between the ATP-binding Lys-65 and alpha-C glutamate (Glu-81), resulting in a partial disordering of the lysine side chain. Also specifically inhibited by erlotinib. Slightly inhibited by gefitinib By similarity.

Subunit structure

Homodimer; homodimerization is required for activation segment autophosphorylation By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein By similarity.

Tissue specificity

Expressed predominantly in lymphoid organs such as spleen, thymus, and bone marrow.

Post-translational modification

Autophosphorylates following homodimerization, leading to activation of the protein By similarity.

Disruption phenotype

Mice do not show any obvious abnormalities. Lymphocytes develop normally but activated lymphocytes show enhanced cell adhesion. Decreased phosphorylation of ERM proteins. Ref.3 Ref.6

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 966966Serine/threonine-protein kinase 10
PRO_0000086698

Regions

Domain36 – 294259Protein kinase
Nucleotide binding42 – 509ATP By similarity
Region175 – 22450Activation segment By similarity
Coiled coil588 – 936349 Potential
Compositional bias749 – 883135Gln-rich

Sites

Active site1571Proton acceptor By similarity
Binding site651ATP By similarity
Binding site1111Inhibitor By similarity
Binding site1131Inhibitor By similarity
Binding site1171Inhibitor; via amide nitrogen By similarity
Binding site1751Inhibitor By similarity

Amino acid modifications

Modified residue131Phosphoserine By similarity
Modified residue1851Phosphothreonine; by autocatalysis By similarity
Modified residue1911Phosphoserine; by autocatalysis By similarity
Modified residue4371Phosphoserine By similarity
Modified residue4431Phosphoserine By similarity
Modified residue4531Phosphoserine By similarity
Modified residue5481Phosphoserine By similarity
Modified residue9501Phosphothreonine Ref.5

Experimental info

Sequence conflict375 – 3773SGS → NGP in BAA24073. Ref.1
Sequence conflict8141K → E in BAA24073. Ref.1
Sequence conflict8631N → H in BAA24073. Ref.1
Sequence conflict8881H → Y in BAA24073. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O55098 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 7A95ABF9D58C8699

FASTA966111,906
        10         20         30         40         50         60 
MAFANFRRIL RLSTFEKRKS REYEHVRRDL DPNDVWEIVG ELGDGAFGKV YKAKNKETGA 

        70         80         90        100        110        120 
LAAAKVIETK SEEELEDYIV EIEILATCDH PYIVKLLGAY YYDGKLWIMI EFCPGGAVDA 

       130        140        150        160        170        180 
IMLELDRGLT EPQIQVVCRQ MLEALNFLHG KRIIHRDLKA GNVLMTLEGD IRLADFGVSA 

       190        200        210        220        230        240 
KNLKTLQKRD SFIGTPYWMA PEVVLCETMK DAPYDYKADI WSLGITLIEM AQIEPPHHEL 

       250        260        270        280        290        300 
NPMRVLLKIA KSDPPTLLTP SKWSVEFRDF LKIALDKNPE TRPSAAQLLQ HPFVSRVTSN 

       310        320        330        340        350        360 
KALRELVAEA KAEVMEEIED GREDGEEEDA VDAVPPLVNH TQDSANVTQP SLDSNKLLQD 

       370        380        390        400        410        420 
SSTPLPPSQP QEPVSGSCSQ PSGDGPLQTT SPADGLSKND NDLKVPVPLR KSRPLSMDAR 

       430        440        450        460        470        480 
IQMDEEKQIP DQDENPSPAA SKSQKANQSR PNSSALETLG GEALTNGGLE LPSSVTPSHS 

       490        500        510        520        530        540 
KRASDCSNLS TSESMDYGTS LSADLSLNKE TGSLSLKGSK LHNKTLKRTR RFVVDGVEVS 

       550        560        570        580        590        600 
ITTSKIISED EKKDEEMRFL RRQELRELRL LQKEEHRNQT QLSSKHELQL EQMHKRFEQE 

       610        620        630        640        650        660 
INAKKKFYDV ELENLERQQK QQVEKMEQDH SVRRKEEAKR IRLEQDRDYA KFQEQLKQMK 

       670        680        690        700        710        720 
KEVKSEVEKL PRQQRKESMK QKMEEHSQKK QRLDRDFVAK QKEDLELAMR KLTTENRREI 

       730        740        750        760        770        780 
CDKERDCLSK KQELLRDREA ALWEMEEHQL QERHQLVKQQ LKDQYFLQRH DLLRKHEKER 

       790        800        810        820        830        840 
EQMQRYNQRM MEQLKVRQQQ EKARLPKIQR SDGKTRMAMY KKSLHINGAG SASEQREKIK 

       850        860        870        880        890        900 
QFSQQEEKRQ KAERLQQQQK HENQMRDMVA QCESNMSELQ QLQNEKCHLL VEHETQKLKA 

       910        920        930        940        950        960 
LDESHNQSLK EWRDKLRPRK KALEEDLNQK KREQEMFFKL SEEAEPRPTT PSKASNFFPY 


SSGDAS 

« Hide

References

« Hide 'large scale' references
[1]"LOK is a novel mouse STE20-like protein kinase that is expressed predominantly in lymphocytes."
Kuramochi S., Moriguchi T., Kuida K., Endo J., Semba K., Nishida E., Karasuyama H.
J. Biol. Chem. 272:22679-22684(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Thymus.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Deficiency of a STE20/PAK family kinase LOK leads to the acceleration of LFA-1 clustering and cell adhesion of activated lymphocytes."
Endo J., Toyama-Sorimachi N., Taya C., Kuramochi-Miyagawa S., Nagata K., Kuida K., Takashi T., Yonekawa H., Yoshizawa Y., Miyasaka N., Karasuyama H.
FEBS Lett. 468:234-238(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-950, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[6]"LOK is a major ERM kinase in resting lymphocytes and regulates cytoskeletal rearrangement through ERM phosphorylation."
Belkina N.V., Liu Y., Hao J.J., Karasuyama H., Shaw S.
Proc. Natl. Acad. Sci. U.S.A. 106:4707-4712(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D89728 mRNA. Translation: BAA24073.1.
AL669844 Genomic DNA. Translation: CAI25517.1.
CCDSCCDS24529.1.
RefSeqNP_033314.2. NM_009288.2.
UniGeneMm.8235.

3D structure databases

ProteinModelPortalO55098.
SMRO55098. Positions 24-415.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO55098. 2 interactions.
MINTMINT-4135895.
STRING10090.ENSMUSP00000099885.

PTM databases

PhosphoSiteO55098.

Proteomic databases

MaxQBO55098.
PaxDbO55098.
PRIDEO55098.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102821; ENSMUSP00000099885; ENSMUSG00000020272.
GeneID20868.
KEGGmmu:20868.
UCSCuc007ijt.1. mouse.

Organism-specific databases

CTD6793.
MGIMGI:1099439. Stk10.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00740000114927.
HOVERGENHBG052712.
InParanoidB1ATW8.
KOK08837.
OMASEEAECP.
OrthoDBEOG7CNZF6.
TreeFamTF351445.

Gene expression databases

BgeeO55098.
CleanExMM_STK10.
GenevestigatorO55098.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR022165. PKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
PF12474. PKK. 2 hits.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSTK10. mouse.
NextBio299697.
PROO55098.
SOURCESearch...

Entry information

Entry nameSTK10_MOUSE
AccessionPrimary (citable) accession number: O55098
Secondary accession number(s): B1ATW8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot