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Protein

Serine/threonine-protein kinase 10

Gene

Stk10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in regulation of lymphocyte migration. Phosphorylates MSN, and possibly PLK1. Involved in regulation of lymphocyte migration by mediating phosphorylation of ERM proteins such as MSN. Acts as a negative regulator of MAP3K1/MEKK1. May also act as a cell cycle regulator by acting as a polo kinase kinase: mediates phosphorylation of PLK1 in vitro; however such data require additional evidences in vivo.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Inhibited by the pyrrole-indolinone inhibitor SU11274 (K00593): intercalates between the ATP-binding Lys-65 and alpha-C glutamate (Glu-81), resulting in a partial disordering of the lysine side chain. Also specifically inhibited by erlotinib. Slightly inhibited by gefitinib (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei65 – 651ATPPROSITE-ProRule annotation
Binding sitei111 – 1111InhibitorBy similarity
Binding sitei113 – 1131InhibitorBy similarity
Binding sitei117 – 1171Inhibitor; via amide nitrogenBy similarity
Active sitei157 – 1571Proton acceptorPROSITE-ProRule annotation
Binding sitei175 – 1751InhibitorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi42 – 509ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 10 (EC:2.7.11.1)
Alternative name(s):
Lymphocyte-oriented kinase
Gene namesi
Name:Stk10
Synonyms:Lok
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1099439. Stk10.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice do not show any obvious abnormalities. Lymphocytes develop normally but activated lymphocytes show enhanced cell adhesion. Decreased phosphorylation of ERM proteins.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 966966Serine/threonine-protein kinase 10PRO_0000086698Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131PhosphoserineBy similarity
Modified residuei185 – 1851Phosphothreonine; by autocatalysisBy similarity
Modified residuei191 – 1911Phosphoserine; by autocatalysisBy similarity
Modified residuei437 – 4371PhosphoserineBy similarity
Modified residuei453 – 4531PhosphoserineBy similarity
Modified residuei484 – 4841PhosphoserineBy similarity
Modified residuei513 – 5131PhosphoserineBy similarity
Modified residuei548 – 5481PhosphoserineBy similarity
Modified residuei950 – 9501Phosphothreonine1 Publication

Post-translational modificationi

Autophosphorylates following homodimerization, leading to activation of the protein.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO55098.
PaxDbiO55098.
PRIDEiO55098.

PTM databases

PhosphoSiteiO55098.

Expressioni

Tissue specificityi

Expressed predominantly in lymphoid organs such as spleen, thymus, and bone marrow.

Gene expression databases

BgeeiO55098.
CleanExiMM_STK10.
GenevisibleiO55098. MM.

Interactioni

Subunit structurei

Homodimer; homodimerization is required for activation segment autophosphorylation.By similarity

Protein-protein interaction databases

IntActiO55098. 3 interactions.
MINTiMINT-4135895.
STRINGi10090.ENSMUSP00000099885.

Structurei

3D structure databases

ProteinModelPortaliO55098.
SMRiO55098. Positions 24-415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 294259Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni175 – 22450Activation segmentBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili588 – 936349Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi749 – 883135Gln-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00800000124019.
HOVERGENiHBG052712.
InParanoidiO55098.
KOiK08837.
OMAiSEEAECP.
OrthoDBiEOG7CNZF6.
TreeFamiTF351445.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR022165. PKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF12474. PKK. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O55098-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFANFRRIL RLSTFEKRKS REYEHVRRDL DPNDVWEIVG ELGDGAFGKV
60 70 80 90 100
YKAKNKETGA LAAAKVIETK SEEELEDYIV EIEILATCDH PYIVKLLGAY
110 120 130 140 150
YYDGKLWIMI EFCPGGAVDA IMLELDRGLT EPQIQVVCRQ MLEALNFLHG
160 170 180 190 200
KRIIHRDLKA GNVLMTLEGD IRLADFGVSA KNLKTLQKRD SFIGTPYWMA
210 220 230 240 250
PEVVLCETMK DAPYDYKADI WSLGITLIEM AQIEPPHHEL NPMRVLLKIA
260 270 280 290 300
KSDPPTLLTP SKWSVEFRDF LKIALDKNPE TRPSAAQLLQ HPFVSRVTSN
310 320 330 340 350
KALRELVAEA KAEVMEEIED GREDGEEEDA VDAVPPLVNH TQDSANVTQP
360 370 380 390 400
SLDSNKLLQD SSTPLPPSQP QEPVSGSCSQ PSGDGPLQTT SPADGLSKND
410 420 430 440 450
NDLKVPVPLR KSRPLSMDAR IQMDEEKQIP DQDENPSPAA SKSQKANQSR
460 470 480 490 500
PNSSALETLG GEALTNGGLE LPSSVTPSHS KRASDCSNLS TSESMDYGTS
510 520 530 540 550
LSADLSLNKE TGSLSLKGSK LHNKTLKRTR RFVVDGVEVS ITTSKIISED
560 570 580 590 600
EKKDEEMRFL RRQELRELRL LQKEEHRNQT QLSSKHELQL EQMHKRFEQE
610 620 630 640 650
INAKKKFYDV ELENLERQQK QQVEKMEQDH SVRRKEEAKR IRLEQDRDYA
660 670 680 690 700
KFQEQLKQMK KEVKSEVEKL PRQQRKESMK QKMEEHSQKK QRLDRDFVAK
710 720 730 740 750
QKEDLELAMR KLTTENRREI CDKERDCLSK KQELLRDREA ALWEMEEHQL
760 770 780 790 800
QERHQLVKQQ LKDQYFLQRH DLLRKHEKER EQMQRYNQRM MEQLKVRQQQ
810 820 830 840 850
EKARLPKIQR SDGKTRMAMY KKSLHINGAG SASEQREKIK QFSQQEEKRQ
860 870 880 890 900
KAERLQQQQK HENQMRDMVA QCESNMSELQ QLQNEKCHLL VEHETQKLKA
910 920 930 940 950
LDESHNQSLK EWRDKLRPRK KALEEDLNQK KREQEMFFKL SEEAEPRPTT
960
PSKASNFFPY SSGDAS
Length:966
Mass (Da):111,906
Last modified:July 27, 2011 - v2
Checksum:i7A95ABF9D58C8699
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti375 – 3773SGS → NGP in BAA24073 (PubMed:9278426).Curated
Sequence conflicti814 – 8141K → E in BAA24073 (PubMed:9278426).Curated
Sequence conflicti863 – 8631N → H in BAA24073 (PubMed:9278426).Curated
Sequence conflicti888 – 8881H → Y in BAA24073 (PubMed:9278426).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89728 mRNA. Translation: BAA24073.1.
AL669844 Genomic DNA. Translation: CAI25517.1.
CCDSiCCDS24529.1.
RefSeqiNP_033314.2. NM_009288.2.
UniGeneiMm.8235.

Genome annotation databases

EnsembliENSMUST00000102821; ENSMUSP00000099885; ENSMUSG00000020272.
GeneIDi20868.
KEGGimmu:20868.
UCSCiuc007ijt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89728 mRNA. Translation: BAA24073.1.
AL669844 Genomic DNA. Translation: CAI25517.1.
CCDSiCCDS24529.1.
RefSeqiNP_033314.2. NM_009288.2.
UniGeneiMm.8235.

3D structure databases

ProteinModelPortaliO55098.
SMRiO55098. Positions 24-415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO55098. 3 interactions.
MINTiMINT-4135895.
STRINGi10090.ENSMUSP00000099885.

PTM databases

PhosphoSiteiO55098.

Proteomic databases

MaxQBiO55098.
PaxDbiO55098.
PRIDEiO55098.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102821; ENSMUSP00000099885; ENSMUSG00000020272.
GeneIDi20868.
KEGGimmu:20868.
UCSCiuc007ijt.1. mouse.

Organism-specific databases

CTDi6793.
MGIiMGI:1099439. Stk10.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00800000124019.
HOVERGENiHBG052712.
InParanoidiO55098.
KOiK08837.
OMAiSEEAECP.
OrthoDBiEOG7CNZF6.
TreeFamiTF351445.

Miscellaneous databases

ChiTaRSiStk10. mouse.
NextBioi299697.
PROiO55098.
SOURCEiSearch...

Gene expression databases

BgeeiO55098.
CleanExiMM_STK10.
GenevisibleiO55098. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR022165. PKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF12474. PKK. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "LOK is a novel mouse STE20-like protein kinase that is expressed predominantly in lymphocytes."
    Kuramochi S., Moriguchi T., Kuida K., Endo J., Semba K., Nishida E., Karasuyama H.
    J. Biol. Chem. 272:22679-22684(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Deficiency of a STE20/PAK family kinase LOK leads to the acceleration of LFA-1 clustering and cell adhesion of activated lymphocytes."
    Endo J., Toyama-Sorimachi N., Taya C., Kuramochi-Miyagawa S., Nagata K., Kuida K., Takashi T., Yonekawa H., Yoshizawa Y., Miyasaka N., Karasuyama H.
    FEBS Lett. 468:234-238(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-950, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  6. "LOK is a major ERM kinase in resting lymphocytes and regulates cytoskeletal rearrangement through ERM phosphorylation."
    Belkina N.V., Liu Y., Hao J.J., Karasuyama H., Shaw S.
    Proc. Natl. Acad. Sci. U.S.A. 106:4707-4712(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiSTK10_MOUSE
AccessioniPrimary (citable) accession number: O55098
Secondary accession number(s): B1ATW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 27, 2011
Last modified: July 22, 2015
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.