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Protein

Dipeptidyl peptidase 3

Gene

Dpp3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves and degrades of the opioid peptide enkephalin (By sinilarity). Also cleaves Arg-Arg-beta-naphthylamide.

Catalytic activityi

Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Enzyme regulationi

Inhibited by spinorphin, an opioid peptide derived from hemoglobin.By similarity

pH dependencei

Optimum pH is 9.0.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi450 – 4501Zinc; catalytic1 Publication
Active sitei451 – 45111 Publication
Metal bindingi455 – 4551Zinc; catalytic1 Publication
Metal bindingi508 – 5081Zinc; catalyticBy similarity

GO - Molecular functioni

  • dipeptidyl-peptidase activity Source: Ensembl
  • metallopeptidase activity Source: UniProtKB-KW
  • zinc ion binding Source: RGD

GO - Biological processi

  • proteolysis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM49.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Dipeptidyl peptidase 3 (EC:3.4.14.4)
Alternative name(s):
Dipeptidyl aminopeptidase III
Dipeptidyl arylamidase III
Dipeptidyl peptidase III
Short name:
DPP III
Enkephalinase B
Gene namesi
Name:Dpp3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi621127. Dpp3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi176 – 1761C → A: 25% to 35% of wild-type activity. 1 Publication
Mutagenesisi176 – 1761C → E or G: Loss of activity. 1 Publication
Mutagenesisi450 – 4501H → Y: Loss of activity and zinc binding. 1 Publication
Mutagenesisi451 – 4511E → A or D: Loss of activity. 1 Publication
Mutagenesisi455 – 4551H → Y: Loss of activity and zinc binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 738737Dipeptidyl peptidase 3PRO_0000078240Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO55096.
PRIDEiO55096.

2D gel databases

World-2DPAGE0004:O55096.

PTM databases

iPTMnetiO55096.
PhosphoSiteiO55096.

Expressioni

Gene expression databases

BgeeiENSRNOG00000031485.
GenevisibleiO55096. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026968.

Structurei

3D structure databases

ProteinModelPortaliO55096.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M49 family.Curated

Phylogenomic databases

eggNOGiKOG3675. Eukaryota.
ENOG410XRK4. LUCA.
GeneTreeiENSGT00390000007335.
HOGENOMiHOG000187784.
HOVERGENiHBG025680.
InParanoidiO55096.
KOiK01277.
OMAiSETWDSK.
OrthoDBiEOG091G0KUS.
PhylomeDBiO55096.

Family and domain databases

InterProiIPR005317. Dipeptidyl-peptase3.
[Graphical view]
PIRSFiPIRSF007828. Dipeptidyl-peptidase_III. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55096-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADTQYILPN DIGVSSLDCR EAFRLLSPTE RLYAHHLSRA AWYGGLAVLL
60 70 80 90 100
QTSPEAPYIY ALLSRLFRAQ DPDQLRQHAL AEGLTEEEYQ AFLVYAAGVY
110 120 130 140 150
SNMGNYKSFG DTKFVPNLPK EKLERVILGS KAAQQHPEEV RSLWQTCGEL
160 170 180 190 200
MFSLEPRLRH LGLGKEGVTT YFSGDCAMED AKLAQDFLDS QNLSAYNTRL
210 220 230 240 250
FKVVGQEGKY HYEVRLASVL NTEPALDSEL TSKLKSYEFQ GNHFQVTRGD
260 270 280 290 300
YAPILQKVVE HLEKAKAYAA NSHQEQMLAQ YVESFTQGSI EAHKRGSRFW
310 320 330 340 350
IQDKGPIVES YIGFIESYRD PFGSRGEFEG FVAMVNKDMS AKFERLVASA
360 370 380 390 400
EQLLKELPWP PAFEKDKFLT PDFTSLDVLT FAGSGIPAGI NIPNYDDLRQ
410 420 430 440 450
TEGFKNVSLG NVLAVAYATK REKLTFMEEE DKDLYIRWKG PSFDVQVGLH
460 470 480 490 500
ELLGHGSGKL FVQDEKGAFN FDQETVINPE TGEQIQSWYR SGETWDSKFS
510 520 530 540 550
TIASSYEECR AESVGLYLCL NPQVLQIFGF EGTDAEDVIY VNWLNMVRAG
560 570 580 590 600
LLALEFYTPE TANWRQAHMQ ARFVILRVLL EAGEGLVTVT PTTGSDGRPD
610 620 630 640 650
ARVHLDRSKI RSVGKPALER FLRRLQVLKS TGDVVAGRAL YEGYAAVTDA
660 670 680 690 700
PPECFLTLRD TVLLRKESRK LIVQPNTRLE GSEVQLVEYE ASAAGLIRSF
710 720 730
CERFPEDGPE LEEVLTQLAT ADAQFWRDQV QEAPSGQA
Length:738
Mass (Da):83,039
Last modified:May 1, 2000 - v2
Checksum:i74BA736BA0806B78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89340 mRNA. Translation: BAA24608.2.
BC107673 mRNA. Translation: AAI07674.1.
RefSeqiNP_446200.1. NM_053748.1.
XP_006230695.1. XM_006230633.2.
UniGeneiRn.10902.
Rn.145904.

Genome annotation databases

EnsembliENSRNOT00000026968; ENSRNOP00000026968; ENSRNOG00000031485.
GeneIDi114591.
KEGGirno:114591.
UCSCiRGD:621127. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89340 mRNA. Translation: BAA24608.2.
BC107673 mRNA. Translation: AAI07674.1.
RefSeqiNP_446200.1. NM_053748.1.
XP_006230695.1. XM_006230633.2.
UniGeneiRn.10902.
Rn.145904.

3D structure databases

ProteinModelPortaliO55096.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026968.

Protein family/group databases

MEROPSiM49.001.

PTM databases

iPTMnetiO55096.
PhosphoSiteiO55096.

2D gel databases

World-2DPAGE0004:O55096.

Proteomic databases

PaxDbiO55096.
PRIDEiO55096.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026968; ENSRNOP00000026968; ENSRNOG00000031485.
GeneIDi114591.
KEGGirno:114591.
UCSCiRGD:621127. rat.

Organism-specific databases

CTDi10072.
RGDi621127. Dpp3.

Phylogenomic databases

eggNOGiKOG3675. Eukaryota.
ENOG410XRK4. LUCA.
GeneTreeiENSGT00390000007335.
HOGENOMiHOG000187784.
HOVERGENiHBG025680.
InParanoidiO55096.
KOiK01277.
OMAiSETWDSK.
OrthoDBiEOG091G0KUS.
PhylomeDBiO55096.

Miscellaneous databases

PROiO55096.

Gene expression databases

BgeeiENSRNOG00000031485.
GenevisibleiO55096. RN.

Family and domain databases

InterProiIPR005317. Dipeptidyl-peptase3.
[Graphical view]
PIRSFiPIRSF007828. Dipeptidyl-peptidase_III. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPP3_RAT
AccessioniPrimary (citable) accession number: O55096
Secondary accession number(s): Q32Q87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.