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Protein

Protein IMPACT

Gene

Impact

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis (PubMed:15937339, PubMed:23447528, PubMed:24333428). May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis (PubMed:15937339, PubMed:23447528). Through its inhibitory action on EIF2AK4/GCN2, plays a role in differentiation of neuronal cells by stimulating neurite outgrowth (PubMed:23447528).3 Publications

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • ribosome binding Source: UniProtKB

GO - Biological processi

  • cellular response to acidic pH Source: UniProtKB
  • cellular response to amino acid starvation Source: UniProtKB
  • cellular response to benomyl Source: UniProtKB
  • cellular response to glucose starvation Source: UniProtKB
  • cellular response to hydrogen peroxide Source: UniProtKB
  • cellular response to leucine starvation Source: UniProtKB
  • cellular response to UV-C Source: UniProtKB
  • negative regulation of cell death Source: UniProtKB
  • negative regulation of protein autophosphorylation Source: UniProtKB
  • negative regulation of protein complex assembly Source: UniProtKB
  • negative regulation of protein phosphorylation Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter in response to stress Source: UniProtKB
  • neuron projection extension Source: UniProtKB
  • positive regulation of neuron differentiation Source: UniProtKB
  • positive regulation of translational initiation in response to starvation Source: UniProtKB
  • regulation of eIF2 alpha phosphorylation by amino acid starvation Source: UniProtKB
  • regulation of translational initiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Differentiation, Neurogenesis, Stress response, Translation regulation

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein IMPACT
Alternative name(s):
Imprinted and ancient gene protein
Gene namesi
Name:Impact
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1098233. Impact.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • polysome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Protein IMPACTPRO_0000330851Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei137 – 1371PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO55091.
MaxQBiO55091.
PaxDbiO55091.
PeptideAtlasiO55091.
PRIDEiO55091.

PTM databases

iPTMnetiO55091.
PhosphoSiteiO55091.

Expressioni

Tissue specificityi

Present in neurons in most areas of the brain. Present at high level in hypothalamus, particularly in the suprachiasmatic nucleus (at protein level) (PubMed:15937339, PubMed:18260151). Preferentially expressed in brain, with a weaker expression in other tissues (PubMed:9256468).3 Publications

Developmental stagei

Detected in embryos at 7, 11, 15, and 17 dpc. At 16 dpc, predominantly expressed in the central nervous system (at protein level). Strongly up-regulated during brain development from 17 dpc up to at least postnatal days 14 (at protein level). In N2a neuroblastoma cell line model and in primary cultures of hippocampal neurons, up-regulated during neuronal differentiation induced by serum reduction (at protein level).2 Publications

Inductioni

Up-regulated after serum withdrawal during neuronal differentiation (PubMed:23447528).1 Publication

Gene expression databases

BgeeiENSMUSG00000024423.
GenevisibleiO55091. MM.

Interactioni

Subunit structurei

Interacts with GCN1; prevents the interaction of GCN1 with EIF2AK4/GCN2 and inhibits EIF2AK4/GCN2 kinase activity (PubMed:15937339, PubMed:22404850). Interaction with RPL39; this interaction occurs in a GCN1-independent manner (PubMed:22404850). Associates with ribosomes; this interaction occurs in a GCN1-independent manner (PubMed:22404850). Associates with actin; this interaction occurs in a GCN1-independent manner (PubMed:22404850).2 Publications

GO - Molecular functioni

  • actin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi200653. 2 interactions.
IntActiO55091. 3 interactions.
MINTiMINT-4129020.
STRINGi10090.ENSMUSP00000025290.

Structurei

3D structure databases

ProteinModelPortaliO55091.
SMRiO55091. Positions 181-273.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 116103RWDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the IMPACT family.Curated
Contains 1 RWD domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3299. Eukaryota.
COG1739. LUCA.
GeneTreeiENSGT00390000017571.
HOGENOMiHOG000160430.
HOVERGENiHBG108005.
InParanoidiO55091.
OMAiIFCIKIS.
OrthoDBiEOG091G0J0V.
PhylomeDBiO55091.
TreeFamiTF314823.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
3.30.230.30. 1 hit.
InterProiIPR023582. Impact.
IPR001498. Impact_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR006575. RWD-domain.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR020569. UPF0029_Impact_CS.
[Graphical view]
PANTHERiPTHR16301. PTHR16301. 1 hit.
PfamiPF05773. RWD. 1 hit.
PF01205. UPF0029. 1 hit.
[Graphical view]
SMARTiSM00591. RWD. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS50908. RWD. 1 hit.
PS00910. UPF0029. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O55091-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEEEVGNSQ RQSEEIEAMA AIYGEEWCVI DENAKIFCIR VTDFMDDPKW
60 70 80 90 100
TLCLQVMLPS EYPGTAPPSY QLNAPWLKGQ ERADLSNSLE EIYVHNMGES
110 120 130 140 150
ILYQWVEKIR DALIQKSQIT EPDPDVKKKT EEVEVESEED PILEHPPENP
160 170 180 190 200
VKTLDLKISE ETQPETEELP PVAHGVPITD RRSTFQAHVA PVVCPEQVKL
210 220 230 240 250
VLAKLYENKK IASATHNIYA YRIFCEDKQT FLQDCEDDGE TAAGGRLLHL
260 270 280 290 300
MEILNVKNVM VVVSRWYGGI LLGPDRFKHI NNCARNILVE KNFTNTPDES
310
TKNLGKKKVK KDKKKNDH
Length:318
Mass (Da):36,276
Last modified:January 1, 1999 - v2
Checksum:iF3A3688D87C88A40
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti303 – 3031N → S in AAG23916 (PubMed:11116084).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87973 mRNA. Translation: BAA35139.1.
AF232228 Genomic DNA. Translation: AAG23916.1.
BC020524 mRNA. Translation: AAH20524.1.
AK138136 mRNA. Translation: BAE23558.1.
CCDSiCCDS29068.1.
RefSeqiNP_032404.1. NM_008378.2.
UniGeneiMm.8154.

Genome annotation databases

EnsembliENSMUST00000025290; ENSMUSP00000025290; ENSMUSG00000024423.
GeneIDi16210.
KEGGimmu:16210.
UCSCiuc008eda.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87973 mRNA. Translation: BAA35139.1.
AF232228 Genomic DNA. Translation: AAG23916.1.
BC020524 mRNA. Translation: AAH20524.1.
AK138136 mRNA. Translation: BAE23558.1.
CCDSiCCDS29068.1.
RefSeqiNP_032404.1. NM_008378.2.
UniGeneiMm.8154.

3D structure databases

ProteinModelPortaliO55091.
SMRiO55091. Positions 181-273.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200653. 2 interactions.
IntActiO55091. 3 interactions.
MINTiMINT-4129020.
STRINGi10090.ENSMUSP00000025290.

PTM databases

iPTMnetiO55091.
PhosphoSiteiO55091.

Proteomic databases

EPDiO55091.
MaxQBiO55091.
PaxDbiO55091.
PeptideAtlasiO55091.
PRIDEiO55091.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025290; ENSMUSP00000025290; ENSMUSG00000024423.
GeneIDi16210.
KEGGimmu:16210.
UCSCiuc008eda.1. mouse.

Organism-specific databases

CTDi55364.
MGIiMGI:1098233. Impact.

Phylogenomic databases

eggNOGiKOG3299. Eukaryota.
COG1739. LUCA.
GeneTreeiENSGT00390000017571.
HOGENOMiHOG000160430.
HOVERGENiHBG108005.
InParanoidiO55091.
OMAiIFCIKIS.
OrthoDBiEOG091G0J0V.
PhylomeDBiO55091.
TreeFamiTF314823.

Miscellaneous databases

PROiO55091.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024423.
GenevisibleiO55091. MM.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
3.30.230.30. 1 hit.
InterProiIPR023582. Impact.
IPR001498. Impact_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR006575. RWD-domain.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR020569. UPF0029_Impact_CS.
[Graphical view]
PANTHERiPTHR16301. PTHR16301. 1 hit.
PfamiPF05773. RWD. 1 hit.
PF01205. UPF0029. 1 hit.
[Graphical view]
SMARTiSM00591. RWD. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS50908. RWD. 1 hit.
PS00910. UPF0029. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIMPCT_MOUSE
AccessioniPrimary (citable) accession number: O55091
Secondary accession number(s): Q3UUR6, Q9EQH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: January 1, 1999
Last modified: September 7, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The Impact locus is imprinted. Paternal inherited gene is expressed, while the maternal inherited gene is silenced. In contrast with most imprinted genes, neighboring genes are apparently not imprinted.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.