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O55082 (PTN20_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein phosphatase non-receptor type 20

EC=3.1.3.48
Alternative name(s):
Testis-specific tyrosine phosphatase
Gene names
Name:Ptpn20
Synonyms:Typ
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length426 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Tyrosine-protein phosphatase targeted to sites of actin polymerization in response of varied extracellular stimuli. Has tyrosine phosphatase activity towards various tyrosyl phosphorylated substrates.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. Ref.1

Subcellular location

Nucleus By similarity. Cytoplasm By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Note: Colocalizes with the microtubule-organizing center and intracellular membrane compartments By similarity.

Tissue specificity

Testis-specific. Specifically expressed in testicular germ cells that undergo meiosis (at protein level). Ref.1

Developmental stage

Detected between 2 and 3 weeks after birth, in parallel with the onset of meiosis. Ref.1

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.

Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
Microtubule
Nucleus
   Coding sequence diversityAlternative splicing
   Molecular functionHydrolase
Protein phosphatase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule organizing center

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein tyrosine phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O55082-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O55082-2)

The sequence of this isoform differs from the canonical sequence as follows:
     122-133: TETSVSEKELTQ → DIYKVSDTWNIY
     134-426: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 426426Tyrosine-protein phosphatase non-receptor type 20
PRO_0000295756

Regions

Domain165 – 418254Tyrosine-protein phosphatase
Region359 – 3657Substrate binding By similarity

Sites

Active site3591Phosphocysteine intermediate By similarity
Binding site3291Substrate By similarity
Binding site4031Substrate By similarity

Amino acid modifications

Modified residue1271Phosphoserine By similarity

Natural variations

Alternative sequence122 – 13312TETSV…KELTQ → DIYKVSDTWNIY in isoform 2.
VSP_027074
Alternative sequence134 – 426293Missing in isoform 2.
VSP_027075

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 2B35FB13379502F4

FASTA42649,119
        10         20         30         40         50         60 
MSSPRKVRGK TGRDNDEEEG NSGNLNLRNS LPSSSQKMTP TKPIFGNKMN SENVKPSHHL 

        70         80         90        100        110        120 
SFSDKYELVY PEPLESDTDE TVWDVSDRSL RNRWNSMDSE TAGPSKTVSP VLSGSSRLSK 

       130        140        150        160        170        180 
DTETSVSEKE LTQLAQIRPL IFNSSARSAM RDCLNTLQKK EELDIIREFL ELEQMTLPDD 

       190        200        210        220        230        240 
FNSGNTLQNR DKNRYRDILP YDSTRVPLGK NKDYINASYI RIVNHEEEYF YIATQGPLPE 

       250        260        270        280        290        300 
TIEDFWQMVL ENNCNVIAMI TREIECGVIK CYSYWPISLK EPLEFEHFSV FLETFHVTQY 

       310        320        330        340        350        360 
FTVRVFQIVK KSTGKSQCVK HLQFTKWPDH GTPASADFFI KYVRYVRKSH ITGPLLVHCS 

       370        380        390        400        410        420 
AGVGRTGVFI CVDVVFSAIE KNYSFDIMNI VTQMRKQRCG MIQTKEQYQF CYEIVLEVLQ 


NLLALY 

« Hide

Isoform 2 [UniParc].

Checksum: 6924B0278635004A
Show »

FASTA13314,972

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of a novel cytoplasmic protein-tyrosine phosphatase that is specifically expressed in spermatocytes."
Ohsugi M., Kuramochi S., Matsuda S., Yamamoto T.
J. Biol. Chem. 272:33092-33099(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME ACTIVITY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Testis.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D64141 mRNA. Translation: BAA23761.1.
AK029493 mRNA. Translation: BAC26476.1.
BC139817 mRNA. Translation: AAI39818.1.
CCDSCCDS36870.1. [O55082-1]
RefSeqNP_033004.1. NM_008978.2. [O55082-1]
UniGeneMm.8019.

3D structure databases

ProteinModelPortalO55082.
SMRO55082. Positions 150-424.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteO55082.

Proteomic databases

PRIDEO55082.

Protocols and materials databases

DNASU19256.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022508; ENSMUSP00000022508; ENSMUSG00000021940. [O55082-1]
GeneID19256.
KEGGmmu:19256.
UCSCuc007szx.1. mouse. [O55082-1]

Organism-specific databases

CTD19256.
MGIMGI:1196295. Ptpn20.

Phylogenomic databases

eggNOGCOG5599.
GeneTreeENSGT00750000117233.
HOGENOMHOG000243992.
HOVERGENHBG095763.
InParanoidO55082.
KOK18039.
OMAAPEPVCC.
OrthoDBEOG7K9K22.
PhylomeDBO55082.
TreeFamTF315573.

Gene expression databases

BgeeO55082.
GenevestigatorO55082.

Family and domain databases

Gene3D3.90.190.10. 1 hit.
InterProIPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00700. PRTYPHPHTASE.
SMARTSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMSSF52799. SSF52799. 1 hit.
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDUSP4. mouse.
NextBio296104.
PROO55082.
SOURCESearch...

Entry information

Entry namePTN20_MOUSE
AccessionPrimary (citable) accession number: O55082
Secondary accession number(s): A4QPF6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: June 1, 1998
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot