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O55082

- PTN20_MOUSE

UniProt

O55082 - PTN20_MOUSE

Protein

Tyrosine-protein phosphatase non-receptor type 20

Gene

Ptpn20

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Tyrosine-protein phosphatase targeted to sites of actin polymerization in response of varied extracellular stimuli. Has tyrosine phosphatase activity towards various tyrosyl phosphorylated substrates.

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 PublicationPROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei329 – 3291SubstrateBy similarity
    Active sitei359 – 3591Phosphocysteine intermediatePROSITE-ProRule annotation
    Binding sitei403 – 4031SubstrateBy similarity

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein phosphatase non-receptor type 20 (EC:3.1.3.48)
    Alternative name(s):
    Testis-specific tyrosine phosphatase
    Gene namesi
    Name:Ptpn20
    Synonyms:Typ
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:1196295. Ptpn20.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
    Note: Colocalizes with the microtubule-organizing center and intracellular membrane compartments.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. microtubule Source: UniProtKB-KW
    3. microtubule organizing center Source: UniProtKB-SubCell
    4. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 426426Tyrosine-protein phosphatase non-receptor type 20PRO_0000295756Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei127 – 1271PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiO55082.

    PTM databases

    PhosphoSiteiO55082.

    Expressioni

    Tissue specificityi

    Testis-specific. Specifically expressed in testicular germ cells that undergo meiosis (at protein level).1 Publication

    Developmental stagei

    Detected between 2 and 3 weeks after birth, in parallel with the onset of meiosis.1 Publication

    Gene expression databases

    BgeeiO55082.
    GenevestigatoriO55082.

    Structurei

    3D structure databases

    ProteinModelPortaliO55082.
    SMRiO55082. Positions 150-424.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini165 – 418254Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni359 – 3657Substrate bindingBy similarity

    Sequence similaritiesi

    Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5599.
    GeneTreeiENSGT00750000117233.
    HOGENOMiHOG000243992.
    HOVERGENiHBG095763.
    InParanoidiO55082.
    KOiK18039.
    OMAiAPEPVCC.
    OrthoDBiEOG7K9K22.
    PhylomeDBiO55082.
    TreeFamiTF315573.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view]
    PfamiPF00102. Y_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00700. PRTYPHPHTASE.
    SMARTiSM00194. PTPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O55082-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSPRKVRGK TGRDNDEEEG NSGNLNLRNS LPSSSQKMTP TKPIFGNKMN    50
    SENVKPSHHL SFSDKYELVY PEPLESDTDE TVWDVSDRSL RNRWNSMDSE 100
    TAGPSKTVSP VLSGSSRLSK DTETSVSEKE LTQLAQIRPL IFNSSARSAM 150
    RDCLNTLQKK EELDIIREFL ELEQMTLPDD FNSGNTLQNR DKNRYRDILP 200
    YDSTRVPLGK NKDYINASYI RIVNHEEEYF YIATQGPLPE TIEDFWQMVL 250
    ENNCNVIAMI TREIECGVIK CYSYWPISLK EPLEFEHFSV FLETFHVTQY 300
    FTVRVFQIVK KSTGKSQCVK HLQFTKWPDH GTPASADFFI KYVRYVRKSH 350
    ITGPLLVHCS AGVGRTGVFI CVDVVFSAIE KNYSFDIMNI VTQMRKQRCG 400
    MIQTKEQYQF CYEIVLEVLQ NLLALY 426
    Length:426
    Mass (Da):49,119
    Last modified:June 1, 1998 - v1
    Checksum:i2B35FB13379502F4
    GO
    Isoform 2 (identifier: O55082-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         122-133: TETSVSEKELTQ → DIYKVSDTWNIY
         134-426: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:133
    Mass (Da):14,972
    Checksum:i6924B0278635004A
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei122 – 13312TETSV…KELTQ → DIYKVSDTWNIY in isoform 2. 1 PublicationVSP_027074Add
    BLAST
    Alternative sequencei134 – 426293Missing in isoform 2. 1 PublicationVSP_027075Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D64141 mRNA. Translation: BAA23761.1.
    AK029493 mRNA. Translation: BAC26476.1.
    BC139817 mRNA. Translation: AAI39818.1.
    CCDSiCCDS36870.1. [O55082-1]
    RefSeqiNP_033004.1. NM_008978.2. [O55082-1]
    UniGeneiMm.8019.

    Genome annotation databases

    EnsembliENSMUST00000022508; ENSMUSP00000022508; ENSMUSG00000021940. [O55082-1]
    GeneIDi19256.
    KEGGimmu:19256.
    UCSCiuc007szx.1. mouse. [O55082-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D64141 mRNA. Translation: BAA23761.1 .
    AK029493 mRNA. Translation: BAC26476.1 .
    BC139817 mRNA. Translation: AAI39818.1 .
    CCDSi CCDS36870.1. [O55082-1 ]
    RefSeqi NP_033004.1. NM_008978.2. [O55082-1 ]
    UniGenei Mm.8019.

    3D structure databases

    ProteinModelPortali O55082.
    SMRi O55082. Positions 150-424.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei O55082.

    Proteomic databases

    PRIDEi O55082.

    Protocols and materials databases

    DNASUi 19256.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022508 ; ENSMUSP00000022508 ; ENSMUSG00000021940 . [O55082-1 ]
    GeneIDi 19256.
    KEGGi mmu:19256.
    UCSCi uc007szx.1. mouse. [O55082-1 ]

    Organism-specific databases

    CTDi 19256.
    MGIi MGI:1196295. Ptpn20.

    Phylogenomic databases

    eggNOGi COG5599.
    GeneTreei ENSGT00750000117233.
    HOGENOMi HOG000243992.
    HOVERGENi HBG095763.
    InParanoidi O55082.
    KOi K18039.
    OMAi APEPVCC.
    OrthoDBi EOG7K9K22.
    PhylomeDBi O55082.
    TreeFami TF315573.

    Miscellaneous databases

    ChiTaRSi DUSP4. mouse.
    NextBioi 296104.
    PROi O55082.
    SOURCEi Search...

    Gene expression databases

    Bgeei O55082.
    Genevestigatori O55082.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view ]
    Pfami PF00102. Y_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00700. PRTYPHPHTASE.
    SMARTi SM00194. PTPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of a novel cytoplasmic protein-tyrosine phosphatase that is specifically expressed in spermatocytes."
      Ohsugi M., Kuramochi S., Matsuda S., Yamamoto T.
      J. Biol. Chem. 272:33092-33099(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME ACTIVITY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Testis.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).

    Entry informationi

    Entry nameiPTN20_MOUSE
    AccessioniPrimary (citable) accession number: O55082
    Secondary accession number(s): A4QPF6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2007
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3