O55082 (PTN20_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase non-receptor type 20
EC=3.1.3.48

Alternative name(s):
Testis-specific tyrosine phosphatase

Gene names

Name:	Ptpn20
Synonyms:	Typ

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tyrosine-protein phosphatase targeted to sites of actin polymerization in response of varied extracellular stimuli. Has tyrosine phosphatase activity towards various tyrosyl phosphorylated substrates.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. Ref.1
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Cytoplasm › cytoskeleton › centrosome By similarity. Note: Colocalizes with the microtubule-organizing center and intracellular membrane compartments By similarity.
Tissue specificity	Testis-specific. Specifically expressed in testicular germ cells that undergo meiosis (at protein level). Ref.1
Developmental stage	Detected between 2 and 3 weeks after birth, in parallel with the onset of meiosis. Ref.1
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O55082-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O55082-2)

The sequence of this isoform differs from the canonical sequence as follows:
     122-133: TETSVSEKELTQ → DIYKVSDTWNIY
     134-426: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Tyrosine-protein phosphatase non-receptor type 20		PRO_0000295756
Regions
	Domain	165 – 418	254	Tyrosine-protein phosphatase
	Region	359 – 365	7	Substrate binding By similarity
Sites
	Active site	359	1	Phosphocysteine intermediate By similarity
	Binding site	329	1	Substrate By similarity
	Binding site	403	1	Substrate By similarity
Natural variations
	Alternative sequence	122 – 133	12	TETSV…KELTQ → DIYKVSDTWNIY in isoform 2.		VSP_027074
	Alternative sequence	134 – 426	293	Missing in isoform 2.		VSP_027075

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 2B35FB13379502F4	FASTA	426	49,119
	10 20 30 40 50 60 MSSPRKVRGK TGRDNDEEEG NSGNLNLRNS LPSSSQKMTP TKPIFGNKMN SENVKPSHHL 70 80 90 100 110 120 SFSDKYELVY PEPLESDTDE TVWDVSDRSL RNRWNSMDSE TAGPSKTVSP VLSGSSRLSK 130 140 150 160 170 180 DTETSVSEKE LTQLAQIRPL IFNSSARSAM RDCLNTLQKK EELDIIREFL ELEQMTLPDD 190 200 210 220 230 240 FNSGNTLQNR DKNRYRDILP YDSTRVPLGK NKDYINASYI RIVNHEEEYF YIATQGPLPE 250 260 270 280 290 300 TIEDFWQMVL ENNCNVIAMI TREIECGVIK CYSYWPISLK EPLEFEHFSV FLETFHVTQY 310 320 330 340 350 360 FTVRVFQIVK KSTGKSQCVK HLQFTKWPDH GTPASADFFI KYVRYVRKSH ITGPLLVHCS 370 380 390 400 410 420 AGVGRTGVFI CVDVVFSAIE KNYSFDIMNI VTQMRKQRCG MIQTKEQYQF CYEIVLEVLQ NLLALY
	Isoform 2 [UniParc]. Checksum: 6924B0278635004A	FASTA	133	14,972
	10 20 30 40 50 60 MSSPRKVRGK TGRDNDEEEG NSGNLNLRNS LPSSSQKMTP TKPIFGNKMN SENVKPSHHL 70 80 90 100 110 120 SFSDKYELVY PEPLESDTDE TVWDVSDRSL RNRWNSMDSE TAGPSKTVSP VLSGSSRLSK 130 DDIYKVSDTW NIY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a novel cytoplasmic protein-tyrosine phosphatase that is specifically expressed in spermatocytes." Ohsugi M., Kuramochi S., Matsuda S., Yamamoto T. J. Biol. Chem. 272:33092-33099(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME ACTIVITY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Testis.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D64141 mRNA. Translation: BAA23761.1. AK029493 mRNA. Translation: BAC26476.1. BC139817 mRNA. Translation: AAI39818.1.
IPI	IPI00124871. IPI00845538.
RefSeq	NP_033004.1. NM_008978.2.
UniGene	Mm.8019.
3D structure databases
HSSP	HSSP built from PDB template 2G59 based on UniProtKB Q16827.
ProteinModelPortal	O55082.
SMR	O55082. Positions 150-424.
ModBase	Search...
PTM databases
PhosphoSite	O55082.
Proteomic databases
PRIDE	O55082.
Protocols and materials databases
DNASU	19256.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022508; ENSMUSP00000022508; ENSMUSG00000021940.
GeneID	19256.
KEGG	mmu:19256.
UCSC	uc007szx.1. mouse.
Organism-specific databases
CTD	19256.
MGI	MGI:1196295. Ptpn20.
Phylogenomic databases
eggNOG	COG5599.
GeneTree	ENSGT00700000104164.
HOGENOM	HOG000243992.
HOVERGEN	HBG095763.
InParanoid	O55082.
OMA	SFNIMDI.
OrthoDB	EOG4M398S.
Gene expression databases
Bgee	O55082.
Genevestigator	O55082.
Family and domain databases
InterPro	IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Pfam	PF00102. Y_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00700. PRTYPHPHTASE.
SMART	SM00194. PTPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	DUSP4. mouse.
NextBio	296104.
SOURCE	Search...

Entry information

Entry name

PTN20_MOUSE

Accession

Primary (citable) accession number: O55082
Secondary accession number(s): A4QPF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program