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Protein

Retinoblastoma-like protein 2

Gene

Rbl2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Repressor
Biological processCell cycle, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Retinoblastoma-like protein 2
Alternative name(s):
130 kDa retinoblastoma-associated protein
Short name:
p130
PPAR-alpha-interacting complex protein 128
Short name:
PRIC128
Retinoblastoma-related protein 2
Short name:
RBR-2
pRb2
Gene namesi
Name:Rbl2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3541 Rbl2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001678431 – 1135Retinoblastoma-like protein 2Add BLAST1135

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei410PhosphoserineBy similarity1
Modified residuei414PhosphothreonineBy similarity1
Modified residuei636PhosphoserineCombined sources1
Modified residuei639PhosphothreonineCombined sources1
Modified residuei659PhosphoserineBy similarity1
Modified residuei669PhosphoserineBy similarity1
Modified residuei684PhosphoserineBy similarity1
Modified residuei942PhosphoserineBy similarity1
Modified residuei946PhosphoserineCombined sources1
Modified residuei960PhosphoserineBy similarity1
Modified residuei965PhosphoserineBy similarity1
Modified residuei967PhosphoserineBy similarity1
Modified residuei968PhosphothreonineBy similarity1
Modified residuei975PhosphoserineBy similarity1
Modified residuei976PhosphoserineBy similarity1
Modified residuei980PhosphothreonineBy similarity1
Modified residuei1031PhosphoserineBy similarity1
Modified residuei1064PhosphoserineCombined sources1
Modified residuei1076PhosphoserineBy similarity1
Modified residuei1108PhosphoserineCombined sources1

Post-translational modificationi

During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-636 and on 5 sites within the domain B. Phosphorylation on Ser-669 in G1 leads to its ubiquitin-dependent proteolysis (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO55081
PRIDEiO55081

PTM databases

iPTMnetiO55081
PhosphoSitePlusiO55081

Interactioni

Subunit structurei

Interacts with AATF, KMT5B and KMT5C. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with USP4 (By similarity). Part of the peroxisome proliferator activated receptor alpha (PPAR-alpha) interacting complex (PRIC). Interacts with RINT1 (By similarity). Interacts with PML (By similarity).By similarity

Protein-protein interaction databases

BioGridi249630, 5 interactors
IntActiO55081, 1 interactor
STRINGi10116.ENSRNOP00000017361

Structurei

3D structure databases

ProteinModelPortaliO55081
SMRiO55081
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni414 – 1021Pocket; binds E1AAdd BLAST608
Regioni414 – 613Domain AAdd BLAST200
Regioni614 – 824SpacerAdd BLAST211
Regioni825 – 1021Domain BAdd BLAST197

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi10 – 13Poly-Pro4
Compositional biasi14 – 17Poly-Ala4
Compositional biasi20 – 24Poly-Glu5
Compositional biasi992 – 995Poly-Glu4

Sequence similaritiesi

Belongs to the retinoblastoma protein (RB) family.Curated

Phylogenomic databases

eggNOGiKOG1010 Eukaryota
ENOG410XQF7 LUCA
HOGENOMiHOG000273892
HOVERGENiHBG017710
InParanoidiO55081
KOiK16332
PhylomeDBiO55081

Family and domain databases

CDDicd00043 CYCLIN, 1 hit
InterProiView protein in InterPro
IPR013763 Cyclin-like
IPR036915 Cyclin-like_sf
IPR028308 RB2
IPR002720 RB_A
IPR002719 RB_B
IPR015030 RB_C
IPR028309 RB_fam
IPR024599 RB_N
PANTHERiPTHR13742 PTHR13742, 1 hit
PTHR13742:SF8 PTHR13742:SF8, 1 hit
PfamiView protein in Pfam
PF11934 DUF3452, 1 hit
PF01858 RB_A, 1 hit
PF01857 RB_B, 1 hit
SMARTiView protein in SMART
SM00385 CYCLIN, 2 hits
SM01367 DUF3452, 1 hit
SM01368 RB_A, 1 hit
SM01369 Rb_C, 1 hit
SUPFAMiSSF47954 SSF47954, 3 hits

Sequencei

Sequence statusi: Complete.

O55081-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGGNQSSP PPPAAAASSE EEEEDGDTAD RAQPAGSPSH QIQQRFEELC
60 70 80 90 100
SRLNMDEAAR AEAWSSYRSM SESYTLEGND LHWLACALYV ACRKSVPTVS
110 120 130 140 150
KGTAEGNYVS LTRILRCSEQ SLIEFFNKMK KWEDMANLPP HFRERTERLE
160 170 180 190 200
RNFTVSAVIF KKYEPIFQDI FKYPQEEQPR QQRGRKQRRQ PCTTSEIFHF
210 220 230 240 250
CWVLFIYAKG NFPMISDDLV NSYHLLLCAL DLVYGNALQC SNRKELVNPN
260 270 280 290 300
FKGVSEDGHP RDSHPSSDPP CVIEKLCSLH DGLVLEAKGI KQHFWKPYIR
310 320 330 340 350
KLFEKKLLRG KEENLTGFLE PGNFAESFKA VNKAYEEYVL ATGSLDERIF
360 370 380 390 400
LGEDAEEEVG TFSRCVSAAS GTESAERTQM RDILQQHLDK SKTLRVCNPL
410 420 430 440 450
TGVRYVQENS PCVTPVSTAT HSLNRLHTML AGLRNAPSEK LEQILRSCSR
460 470 480 490 500
DPTRAIADRL REMYEIYSQH FQPDENVSNC AKEMANKHFR FAEMLYYKVL
510 520 530 540 550
ESVIEQEQKR LGDMDLSGVL EQDAFHKSLL ACCLEVVAFS YKPPGNFPFI
560 570 580 590 600
AEIFDVPHYH FYKVIEVFIR AEDGLCREVV KHLNQIEEQI LDHLAWKTKS
610 620 630 640 650
PLWDRIRDNE NRVPTCEEVT PPHNLERTDE IYIAGSPLTP RRVGEVRTDA
660 670 680 690 700
GGLGRSVTSP TTLYDRYSSP TVSTTRRRLF ESDSPSEGST AGRIPPQPLV
710 720 730 740 750
NAVPVQNVSG ETVSVTPVPG QTLVTMATAT VTANNGQTVT IPVQGIANEN
760 770 780 790 800
GGITFFPVQV NVGGQAQAVT GSIQPLSAQA LAGSLSSQQV TGTTLQVPGP
810 820 830 840 850
VAIQQISPGG QQQNQGQPLT SSSIRPRKTS SLSLFFRKVY YLAGVRLRDL
860 870 880 890 900
CTKLDISDEL RKKIWTCFEF SIVQCPELMM DRHLDQLLMC AIYVMAKVTK
910 920 930 940 950
EDKSFQNIMR CYRTQPQARS QVYRSVLIKG KRRNSGSCEN RSHQNSPTEL
960 970 980 990 1000
NTDRASRDSS PVMRSNSTLP VPQPSSAPPT PTRLTGANSD IEEEERGDLI
1010 1020 1030 1040 1050
QFYNNIYRKQ IQTFAMKYSQ ANSQMDTPPL SPYPFVRTGS PRRVQLSQSH
1060 1070 1080 1090 1100
PIYISPHKNE AMLSPREKIF YYFSNSPSKR LREINSMIRT GETPTKKRGI
1110 1120 1130
LLDDGSESPA KRICPENHSA LLRRLQDVAN DRGSH
Length:1,135
Mass (Da):127,818
Last modified:June 1, 1998 - v1
Checksum:i6C42A87E163B4298
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D55627 mRNA Translation: BAA24196.1
RefSeqiNP_112356.1, NM_031094.1
UniGeneiRn.11020

Genome annotation databases

GeneIDi81758
KEGGirno:81758

Similar proteinsi

Entry informationi

Entry nameiRBL2_RAT
AccessioniPrimary (citable) accession number: O55081
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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