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Protein

Deoxyribonuclease gamma

Gene

Dnase1l3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends (By similarity). Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA (PubMed:15796714, PubMed:19154352, PubMed:12095301). Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis. The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells (PubMed:12050166, PubMed:15167901, PubMed:17218958, PubMed:24312463). Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity (PubMed:15796714, PubMed:27293190).By similarity1 Publication5 Publications

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarity
  • Mg2+By similarity

Enzyme regulationi

Inhibited by zinc. Inhibited by heparin and proteolysis by plasmin.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei105By similarity1
Active sitei160By similarity1

GO - Molecular functioni

  • DNA binding Source: Ensembl
  • endodeoxyribonuclease activity Source: Ensembl
  • endonuclease activity Source: MGI

GO - Biological processi

  • apoptotic DNA fragmentation Source: MGI
  • programmed cell death involved in cell development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Apoptosis, Necrosis

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BRENDAi3.1.21.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribonuclease gamma (EC:3.1.21.-)
Short name:
DNase gamma
Alternative name(s):
DNase I homolog protein DHP2
Deoxyribonuclease I-like 3
Short name:
DNase I-like 3
Liver and spleen DNase
Short name:
LS-DNase
Short name:
LSD
Gene namesi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1314633. Dnase1l3.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: MGI
  • extracellular region Source: UniProtKB-SubCell
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25By similarityAdd BLAST25
ChainiPRO_000000728926 – 310Deoxyribonuclease gammaAdd BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi199 ↔ 236Essential for enzymatic activityBy similarity

Post-translational modificationi

Poly-ADP-ribosylated by PARP1. ADP-ribosylation negatively regulates enzymatic activity during apoptosis.By similarity

Keywords - PTMi

ADP-ribosylation, Disulfide bond

Proteomic databases

PaxDbiO55070.
PRIDEiO55070.

PTM databases

PhosphoSitePlusiO55070.

Expressioni

Tissue specificityi

Expressed at high levels in liver, spleen and testes. Expressed at lower levels in heart, lungs, skeletal muscle and kidney. Not expressed in brain. Predominantly expressed in macrophages; at protein level. Secreted by mononuclear phagocytes.4 Publications

Developmental stagei

Expression is first detected at embryonic day 11, and higher amounts were detected at days 15 and 17.1 Publication

Gene expression databases

BgeeiENSMUSG00000025279.
CleanExiMM_DNASE1L3.
GenevisibleiO55070. MM.

Structurei

3D structure databases

ProteinModelPortaliO55070.
SMRiO55070.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni289 – 310Not required for free DNA-nuclease activity but required for activity towards liposome-coated DNA1 PublicationAdd BLAST22

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi40 – 56Bipartite nuclear localization signalSequence analysisAdd BLAST17
Motifi301 – 307Nuclear localization signalSequence analysis7

Sequence similaritiesi

Belongs to the DNase I family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHHA. Eukaryota.
ENOG410ZR9A. LUCA.
GeneTreeiENSGT00390000013146.
HOGENOMiHOG000059570.
HOVERGENiHBG051368.
InParanoidiO55070.
KOiK11995.
OMAiQSPHTAV.
OrthoDBiEOG091G0I0B.
PhylomeDBiO55070.
TreeFamiTF329541.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR018057. Deoxyribonuclease-1_AS.
IPR016202. DNase_I.
IPR033125. DNASE_I_2.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERiPTHR11371. PTHR11371. 1 hit.
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
PIRSFiPIRSF000988. DNase_I_euk. 1 hit.
PRINTSiPR00130. DNASEI.
SMARTiSM00476. DNaseIc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
PROSITEiPS00919. DNASE_I_1. 1 hit.
PS00918. DNASE_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55070-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLHPASPRL ASLLLFILAL HDTLALRLCS FNVRSFGASK KENHEAMDII
60 70 80 90 100
VKIIKRCDLI LLMEIKDSSN NICPMLMEKL NGNSRRSTTY NYVISSRLGR
110 120 130 140 150
NTYKEQYAFV YKEKLVSVKT KYHYHDYQDG DTDVFSREPF VVWFHSPFTA
160 170 180 190 200
VKDFVIVPLH TTPETSVKEI DELVDVYTDV RSQWKTENFI FMGDFNAGCS
210 220 230 240 250
YVPKKAWQNI RLRTDPKFVW LIGDQEDTTV KKSTSCAYDR IVLCGQEIVN
260 270 280 290 300
SVVPRSSGVF DFQKAYDLSE EEALDVSDHF PVEFKLQSSR AFTNNRKSVS
310
LKKRKKGNRS
Length:310
Mass (Da):35,760
Last modified:June 1, 1998 - v1
Checksum:iBE483821E045E374
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti89T → I in strain: DBA/2, NZB and NZW, in vitro decreases nuclease activity against free DNA by approximately twofold and decreases activity to establish a barrier to liposomal gene transfection by eightfold. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047355 mRNA. Translation: AAC35753.1.
U76110 mRNA. Translation: AAD09222.1.
AY024355 Genomic DNA. Translation: AAK07733.1.
BC012671 mRNA. Translation: AAH12671.1.
CCDSiCCDS26807.1.
RefSeqiNP_031896.3. NM_007870.3.
XP_006517984.1. XM_006517921.3.
UniGeneiMm.272258.
Mm.490513.

Genome annotation databases

EnsembliENSMUST00000026315; ENSMUSP00000026315; ENSMUSG00000025279.
GeneIDi13421.
KEGGimmu:13421.
UCSCiuc007sem.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047355 mRNA. Translation: AAC35753.1.
U76110 mRNA. Translation: AAD09222.1.
AY024355 Genomic DNA. Translation: AAK07733.1.
BC012671 mRNA. Translation: AAH12671.1.
CCDSiCCDS26807.1.
RefSeqiNP_031896.3. NM_007870.3.
XP_006517984.1. XM_006517921.3.
UniGeneiMm.272258.
Mm.490513.

3D structure databases

ProteinModelPortaliO55070.
SMRiO55070.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSitePlusiO55070.

Proteomic databases

PaxDbiO55070.
PRIDEiO55070.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026315; ENSMUSP00000026315; ENSMUSG00000025279.
GeneIDi13421.
KEGGimmu:13421.
UCSCiuc007sem.1. mouse.

Organism-specific databases

CTDi1776.
MGIiMGI:1314633. Dnase1l3.

Phylogenomic databases

eggNOGiENOG410IHHA. Eukaryota.
ENOG410ZR9A. LUCA.
GeneTreeiENSGT00390000013146.
HOGENOMiHOG000059570.
HOVERGENiHBG051368.
InParanoidiO55070.
KOiK11995.
OMAiQSPHTAV.
OrthoDBiEOG091G0I0B.
PhylomeDBiO55070.
TreeFamiTF329541.

Enzyme and pathway databases

BRENDAi3.1.21.1. 3474.

Miscellaneous databases

PROiO55070.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025279.
CleanExiMM_DNASE1L3.
GenevisibleiO55070. MM.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR018057. Deoxyribonuclease-1_AS.
IPR016202. DNase_I.
IPR033125. DNASE_I_2.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERiPTHR11371. PTHR11371. 1 hit.
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
PIRSFiPIRSF000988. DNase_I_euk. 1 hit.
PRINTSiPR00130. DNASEI.
SMARTiSM00476. DNaseIc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
PROSITEiPS00919. DNASE_I_1. 1 hit.
PS00918. DNASE_I_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNSL3_MOUSE
AccessioniPrimary (citable) accession number: O55070
Secondary accession number(s): Q91X38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.