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Protein

Thiopurine S-methyltransferase

Gene

Tpmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.

Catalytic activityi

S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35Substrate1
Binding sitei64S-adenosyl-L-methionine; via carbonyl oxygen1
Binding sitei85S-adenosyl-L-methionine1
Binding sitei147S-adenosyl-L-methionine1

GO - Molecular functioni

  • thiopurine S-methyltransferase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.67. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiopurine S-methyltransferase (EC:2.1.1.67)
Alternative name(s):
Thiopurine methyltransferase
Gene namesi
Name:Tpmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:98812. Tpmt.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002201051 – 240Thiopurine S-methyltransferaseAdd BLAST240

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineCombined sources1
Modified residuei53N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO55060.
PaxDbiO55060.
PeptideAtlasiO55060.
PRIDEiO55060.

PTM databases

iPTMnetiO55060.
PhosphoSitePlusiO55060.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021376.
CleanExiMM_TPMT.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

IntActiO55060. 2 interactors.
MINTiMINT-1855972.
STRINGi10090.ENSMUSP00000021806.

Structurei

Secondary structure

1240
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni11 – 16Combined sources6
Helixi21 – 30Combined sources10
Beta strandi38 – 40Combined sources3
Helixi42 – 52Combined sources11
Beta strandi59 – 62Combined sources4
Helixi70 – 76Combined sources7
Beta strandi80 – 84Combined sources5
Helixi88 – 97Combined sources10
Beta strandi102 – 106Combined sources5
Beta strandi114 – 118Combined sources5
Beta strandi121 – 128Combined sources8
Turni130 – 132Combined sources3
Helixi133 – 136Combined sources4
Beta strandi141 – 149Combined sources9
Turni150 – 152Combined sources3
Helixi155 – 157Combined sources3
Helixi158 – 167Combined sources10
Beta strandi169 – 181Combined sources13
Helixi184 – 186Combined sources3
Beta strandi189 – 191Combined sources3
Helixi196 – 203Combined sources8
Turni204 – 206Combined sources3
Beta strandi207 – 216Combined sources10
Helixi220 – 222Combined sources3
Turni223 – 226Combined sources4
Beta strandi231 – 239Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GB4X-ray1.25A/B1-240[»]
3BGDX-ray2.00A/B1-240[»]
3BGIX-ray1.80A/B1-240[»]
ProteinModelPortaliO55060.
SMRiO55060.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO55060.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 35S-adenosyl-L-methionine bindingAdd BLAST12
Regioni129 – 130S-adenosyl-L-methionine binding2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IGTR. Eukaryota.
ENOG4111GNF. LUCA.
HOGENOMiHOG000276919.
HOVERGENiHBG003037.
InParanoidiO55060.
KOiK00569.
PhylomeDBiO55060.
TreeFamiTF328951.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00812. Thiopur_methtran. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR025835. Thiopurine_S-MeTrfase.
IPR008854. TPMT.
[Graphical view]
PfamiPF05724. TPMT. 1 hit.
[Graphical view]
PIRSFiPIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51585. SAM_MT_TPMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O55060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLDMKEHPD AEVQKNQVLT LEDWKEKWVT RHISFHQEQG HQLLKKHLDT
60 70 80 90 100
FLKGQSGLRV FFPLCGKAIE MKWFADRGHT VVGVEISEIG IREFFAEQNL
110 120 130 140 150
SYTEEPLAEI AGAKVFKSSS GSISLYCCSI FDLPRANIGK FDRIWDRGAL
160 170 180 190 200
VAINPGDHDR YADIILSLLR KEFQYLVAVL SYDPTKHAGP PFYVPSAELK
210 220 230 240
RLFGTKCSMQ CLEEVDALEE RHKAWGLDYL FEKLYLLTEK
Length:240
Mass (Da):27,586
Last modified:June 1, 1998 - v1
Checksum:i2BA57F30E8EB72D2
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti69I → V in strain: C57BL/6J. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF046887 mRNA. Translation: AAC25919.1.
AF037043 mRNA. Translation: AAD02092.1.
AF037044 mRNA. Translation: AAD02093.1.
AF104832
, AF104825, AF104826, AF104827, AF104828, AF104829, AF104830, AF104831 Genomic DNA. Translation: AAF06075.1.
BC021598 mRNA. Translation: AAH21598.1.
AH009424 Genomic DNA. Translation: AAF74424.1.
CCDSiCCDS26488.1.
RefSeqiNP_058065.2. NM_016785.2.
XP_006516989.1. XM_006516926.2.
UniGeneiMm.10169.

Genome annotation databases

GeneIDi22017.
KEGGimmu:22017.
UCSCiuc007qhq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF046887 mRNA. Translation: AAC25919.1.
AF037043 mRNA. Translation: AAD02092.1.
AF037044 mRNA. Translation: AAD02093.1.
AF104832
, AF104825, AF104826, AF104827, AF104828, AF104829, AF104830, AF104831 Genomic DNA. Translation: AAF06075.1.
BC021598 mRNA. Translation: AAH21598.1.
AH009424 Genomic DNA. Translation: AAF74424.1.
CCDSiCCDS26488.1.
RefSeqiNP_058065.2. NM_016785.2.
XP_006516989.1. XM_006516926.2.
UniGeneiMm.10169.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GB4X-ray1.25A/B1-240[»]
3BGDX-ray2.00A/B1-240[»]
3BGIX-ray1.80A/B1-240[»]
ProteinModelPortaliO55060.
SMRiO55060.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO55060. 2 interactors.
MINTiMINT-1855972.
STRINGi10090.ENSMUSP00000021806.

PTM databases

iPTMnetiO55060.
PhosphoSitePlusiO55060.

Proteomic databases

MaxQBiO55060.
PaxDbiO55060.
PeptideAtlasiO55060.
PRIDEiO55060.

Protocols and materials databases

DNASUi22017.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi22017.
KEGGimmu:22017.
UCSCiuc007qhq.2. mouse.

Organism-specific databases

CTDi7172.
MGIiMGI:98812. Tpmt.

Phylogenomic databases

eggNOGiENOG410IGTR. Eukaryota.
ENOG4111GNF. LUCA.
HOGENOMiHOG000276919.
HOVERGENiHBG003037.
InParanoidiO55060.
KOiK00569.
PhylomeDBiO55060.
TreeFamiTF328951.

Enzyme and pathway databases

BRENDAi2.1.1.67. 3474.

Miscellaneous databases

EvolutionaryTraceiO55060.
PROiO55060.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021376.
CleanExiMM_TPMT.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00812. Thiopur_methtran. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR025835. Thiopurine_S-MeTrfase.
IPR008854. TPMT.
[Graphical view]
PfamiPF05724. TPMT. 1 hit.
[Graphical view]
PIRSFiPIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51585. SAM_MT_TPMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPMT_MOUSE
AccessioniPrimary (citable) accession number: O55060
Secondary accession number(s): Q9JIL7, Q9QUG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.