Reviewed, UniProtKB/Swiss-Prot O55055 (TRDMT_MOUSE)

Names and origin

Protein names

Recommended name:
    tRNA (cytosine-5-)-methyltransferase
    EC=2.1.1.29
Alternative name(s):
    DNA (cytosine-5)-methyltransferase-like protein 2
      Short name=Dnmt2
    DNA methyltransferase homolog MmuIIP
      Short name=DNA MTase homolog MmuIIP
      Short name=M.MmuIIP
    Met-2

Gene names

Name:	Trdmt1
Synonyms:	Dnmt2, Met2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp) By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylcytosine.
Subcellular location	Nucleus Probable.
Tissue specificity	Highly expressed in thymus, testis, and at much lower levels in spleen, lung, brain, heart, kidney, liver, skeletal muscle and embryonic stem cells. Ref.2
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	tRNA (cytosine-5-)-methyltransferase		PRO_0000088041
Sites
	Active site	79	1	By similarity
Experimental info
	Sequence conflict	164	1	F → S in AAC40130. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O55055-1 [UniParc]. Last modified February 21, 2002. Version 2. Checksum: 14FEF78C0D2F8BFB	FASTA	415	46,794
	10 20 30 40 50 60 MEPLRVLELY SGIGGMHHAL RESHIPAHVV AAIDVNTVAN EVYKHNFPHT HLLSKTIEGI 70 80 90 100 110 120 SLEDFDKLSF NMILMSPPCQ PFTRIGLQGD MTDPRTTSFL YILDILPRLQ KLPKYILLEN 130 140 150 160 170 180 VKGFEVSSTR GLLIQTIEAC GFQYQEFLLS PSSLGIPNSR LRYFLIAKLQ SEPFPFQAPG 190 200 210 220 230 240 QILMEFPKIV TVEPQKYAVV EESQPRVQRT GPRICAESSS TQSSGKDTIL FKLETVEERD 250 260 270 280 290 300 RKHQQDSDLS VQMLKDFLED GDTDEYLLPP KLLLRYALLL DIVKPTSRRS MCFTKGYGSY 310 320 330 340 350 360 IEGTGSVLQA AEDAQIENIY KSLPDLPPEE KIAKLSMLKL RYFTPKEIAN LQGFPPEFGF 370 380 390 400 410 PEKTTVKQRY RLLGNSLNVH VVAKLLTVLC EGFGNASESC HKMPLILDSN SKILS

References

[1]	"A candidate mammalian DNA methyltransferase related to pmt1p of fission yeast." Yoder J.A., Bestor T.H. Hum. Mol. Genet. 7:279-284(1998) [PubMed: 9425235] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells." Okano M., Xie S., Li E. Nucleic Acids Res. 26:2536-2540(1998) [PubMed: 9592134] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Embryonic stem cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF012129 mRNA. Translation: AAC53529.1. AF045889 mRNA. Translation: AAC40130.1.
IPI	IPI00115186.
RefSeq	NP_034197.3.
UniGene	Mm.6979
3D structure databases
HSSP	HSSP built from PDB template 1G55 based on UniProtKB O14717.
ModBase	Search...
Proteomic databases
PRIDE	O55055.
Genome annotation databases
Ensembl	ENSMUSG00000026723. Mus musculus. [Contig view]
GeneID	13434.
KEGG	mmu:13434.
Organism-specific databases
MGI	MGI:1274787. Trdmt1.
Phylogenomic databases
HOVERGEN	O55055.
OMA	O55055. YFTPREV.
Enzyme and pathway databases
BRENDA	2.1.1.29. 244. 2.1.1.37. 244.
Gene expression databases
ArrayExpress	O55055.
Bgee	O55055.
CleanEx	MM_TRDMT1.
GermOnline	ENSMUSG00000026723. Mus musculus.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 2 hits. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
PROSITE	PS00094. C5_MTASE_1. False negative. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	283863.
SOURCE	Search...

Entry information

Entry name

TRDMT_MOUSE

Accession

Primary (citable) accession number: O55055
Secondary accession number(s): O35212

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	February 21, 2002
Last modified:	June 16, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)