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Protein

tRNA (cytosine(38)-C(5))-methyltransferase

Gene

Trdmt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).By similarity

Catalytic activityi

S-adenosyl-L-methionine + cytosine(38) in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(38) in tRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34S-adenosyl-L-methionineBy similarity1
Binding sitei76S-adenosyl-L-methionineBy similarity1
Active sitei79PROSITE-ProRule annotation1
Binding sitei376S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

  • RNA binding Source: UniProtKB-KW
  • tRNA (cytosine-5-)-methyltransferase activity Source: MGI
  • tRNA methyltransferase activity Source: MGI

GO - Biological processi

  • response to amphetamine Source: Ensembl
  • tRNA methylation Source: MGI

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.37. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytosine(38)-C(5))-methyltransferase (EC:2.1.1.204)
Alternative name(s):
DNA (cytosine-5)-methyltransferase-like protein 2
Short name:
Dnmt2
DNA methyltransferase homolog MmuIIP
Short name:
DNA MTase homolog MmuIIP
Short name:
M.MmuIIP
Met-2
Gene namesi
Name:Trdmt1
Synonyms:Dnmt2, Met2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1274787. Trdmt1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000880411 – 415tRNA (cytosine(38)-C(5))-methyltransferaseAdd BLAST415

Proteomic databases

PaxDbiO55055.
PRIDEiO55055.

PTM databases

iPTMnetiO55055.
PhosphoSitePlusiO55055.

Expressioni

Tissue specificityi

Highly expressed in thymus, testis, and at much lower levels in spleen, lung, brain, heart, kidney, liver, skeletal muscle and embryonic stem cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000026723.
CleanExiMM_TRDMT1.
ExpressionAtlasiO55055. baseline and differential.
GenevisibleiO55055. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000114572.

Structurei

3D structure databases

ProteinModelPortaliO55055.
SMRiO55055.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 396SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST393

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 15S-adenosyl-L-methionine bindingBy similarity3
Regioni57 – 58S-adenosyl-L-methionine bindingBy similarity2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0919. Eukaryota.
COG0270. LUCA.
GeneTreeiENSGT00390000016416.
HOGENOMiHOG000265038.
HOVERGENiHBG051385.
InParanoidiO55055.
KOiK15336.
OMAiLRYFTPK.
OrthoDBiEOG091G0FXV.
PhylomeDBiO55055.
TreeFamiTF300024.

Family and domain databases

InterProiView protein in InterPro
IPR001525. C5_MeTfrase.
IPR031303. C5_meth_CS.
IPR029063. SAM-dependent_MTases.
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiView protein in PROSITE
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.

Sequencei

Sequence statusi: Complete.

O55055-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPLRVLELY SGIGGMHHAL RESHIPAHVV AAIDVNTVAN EVYKHNFPHT
60 70 80 90 100
HLLSKTIEGI SLEDFDKLSF NMILMSPPCQ PFTRIGLQGD MTDPRTTSFL
110 120 130 140 150
YILDILPRLQ KLPKYILLEN VKGFEVSSTR GLLIQTIEAC GFQYQEFLLS
160 170 180 190 200
PSSLGIPNSR LRYFLIAKLQ SEPFPFQAPG QILMEFPKIV TVEPQKYAVV
210 220 230 240 250
EESQPRVQRT GPRICAESSS TQSSGKDTIL FKLETVEERD RKHQQDSDLS
260 270 280 290 300
VQMLKDFLED GDTDEYLLPP KLLLRYALLL DIVKPTSRRS MCFTKGYGSY
310 320 330 340 350
IEGTGSVLQA AEDAQIENIY KSLPDLPPEE KIAKLSMLKL RYFTPKEIAN
360 370 380 390 400
LQGFPPEFGF PEKTTVKQRY RLLGNSLNVH VVAKLLTVLC EGFGNASESC
410
HKMPLILDSN SKILS
Length:415
Mass (Da):46,794
Last modified:February 21, 2002 - v2
Checksum:i14FEF78C0D2F8BFB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti164F → S in AAC40130 (PubMed:9592134).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012129 mRNA. Translation: AAC53529.1.
AF045889 mRNA. Translation: AAC40130.1.
CCDSiCCDS15695.1.
RefSeqiNP_034197.3. NM_010067.4.
UniGeneiMm.6979.

Genome annotation databases

EnsembliENSMUST00000124488; ENSMUSP00000114572; ENSMUSG00000026723.
GeneIDi13434.
KEGGimmu:13434.
UCSCiuc008ika.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiTRDMT_MOUSE
AccessioniPrimary (citable) accession number: O55055
Secondary accession number(s): O35212
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: February 21, 2002
Last modified: October 25, 2017
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families