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O55055 (TRDMT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (cytosine(38)-C(5))-methyltransferase

EC=2.1.1.204
Alternative name(s):
DNA (cytosine-5)-methyltransferase-like protein 2
Short name=Dnmt2
DNA methyltransferase homolog MmuIIP
Short name=DNA MTase homolog MmuIIP
Short name=M.MmuIIP
Met-2
Gene names
Name:Trdmt1
Synonyms:Dnmt2, Met2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp) By similarity.

Catalytic activity

S-adenosyl-L-methionine + cytosine38 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA.

Subcellular location

Nucleus Probable.

Tissue specificity

Highly expressed in thymus, testis, and at much lower levels in spleen, lung, brain, heart, kidney, liver, skeletal muscle and embryonic stem cells. Ref.2

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415tRNA (cytosine(38)-C(5))-methyltransferase
PRO_0000088041

Regions

Domain4 – 396393SAM-dependent MTase C5-type

Sites

Active site791 By similarity

Experimental info

Sequence conflict1641F → S in AAC40130. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O55055 [UniParc].

Last modified February 21, 2002. Version 2.
Checksum: 14FEF78C0D2F8BFB

FASTA41546,794
        10         20         30         40         50         60 
MEPLRVLELY SGIGGMHHAL RESHIPAHVV AAIDVNTVAN EVYKHNFPHT HLLSKTIEGI 

        70         80         90        100        110        120 
SLEDFDKLSF NMILMSPPCQ PFTRIGLQGD MTDPRTTSFL YILDILPRLQ KLPKYILLEN 

       130        140        150        160        170        180 
VKGFEVSSTR GLLIQTIEAC GFQYQEFLLS PSSLGIPNSR LRYFLIAKLQ SEPFPFQAPG 

       190        200        210        220        230        240 
QILMEFPKIV TVEPQKYAVV EESQPRVQRT GPRICAESSS TQSSGKDTIL FKLETVEERD 

       250        260        270        280        290        300 
RKHQQDSDLS VQMLKDFLED GDTDEYLLPP KLLLRYALLL DIVKPTSRRS MCFTKGYGSY 

       310        320        330        340        350        360 
IEGTGSVLQA AEDAQIENIY KSLPDLPPEE KIAKLSMLKL RYFTPKEIAN LQGFPPEFGF 

       370        380        390        400        410 
PEKTTVKQRY RLLGNSLNVH VVAKLLTVLC EGFGNASESC HKMPLILDSN SKILS 

« Hide

References

[1]"A candidate mammalian DNA methyltransferase related to pmt1p of fission yeast."
Yoder J.A., Bestor T.H.
Hum. Mol. Genet. 7:279-284(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells."
Okano M., Xie S., Li E.
Nucleic Acids Res. 26:2536-2540(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Embryonic stem cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF012129 mRNA. Translation: AAC53529.1.
AF045889 mRNA. Translation: AAC40130.1.
RefSeqNP_034197.3. NM_010067.4.
UniGeneMm.6979.

3D structure databases

ProteinModelPortalO55055.
SMRO55055. Positions 2-391.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteO55055.

Proteomic databases

PaxDbO55055.
PRIDEO55055.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000124488; ENSMUSP00000114572; ENSMUSG00000026723.
GeneID13434.
KEGGmmu:13434.
UCSCuc008ika.2. mouse.

Organism-specific databases

CTD1787.
MGIMGI:1274787. Trdmt1.

Phylogenomic databases

eggNOGCOG0270.
HOGENOMHOG000265038.
HOVERGENHBG051385.
InParanoidO55055.
KOK15336.
OMATCCFTRG.
OrthoDBEOG7PZRZ4.
PhylomeDBO55055.
TreeFamTF300024.

Enzyme and pathway databases

BRENDA2.1.1.37. 3474.

Gene expression databases

ArrayExpressO55055.
BgeeO55055.
CleanExMM_TRDMT1.
GenevestigatorO55055.

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR025813. DNA/tRNA_C5-MeTrfase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio283863.
PROO55055.
SOURCESearch...

Entry information

Entry nameTRDMT_MOUSE
AccessionPrimary (citable) accession number: O55055
Secondary accession number(s): O35212
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: February 21, 2002
Last modified: April 16, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot