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O55055

- TRDMT_MOUSE

UniProt

O55055 - TRDMT_MOUSE

Protein

tRNA (cytosine(38)-C(5))-methyltransferase

Gene

Trdmt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (21 Feb 2002)
      Previous versions | rss
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    Functioni

    Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + cytosine(38) in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(38) in tRNA.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei79 – 791PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. RNA binding Source: UniProtKB-KW
    3. tRNA methyltransferase activity Source: MGI

    GO - Biological processi

    1. DNA methylation Source: InterPro
    2. response to amphetamine Source: Ensembl
    3. tRNA methylation Source: MGI

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BRENDAi2.1.1.37. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (cytosine(38)-C(5))-methyltransferase (EC:2.1.1.204)
    Alternative name(s):
    DNA (cytosine-5)-methyltransferase-like protein 2
    Short name:
    Dnmt2
    DNA methyltransferase homolog MmuIIP
    Short name:
    DNA MTase homolog MmuIIP
    Short name:
    M.MmuIIP
    Met-2
    Gene namesi
    Name:Trdmt1
    Synonyms:Dnmt2, Met2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1274787. Trdmt1.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 415415tRNA (cytosine(38)-C(5))-methyltransferasePRO_0000088041Add
    BLAST

    Proteomic databases

    PaxDbiO55055.
    PRIDEiO55055.

    PTM databases

    PhosphoSiteiO55055.

    Expressioni

    Tissue specificityi

    Highly expressed in thymus, testis, and at much lower levels in spleen, lung, brain, heart, kidney, liver, skeletal muscle and embryonic stem cells.1 Publication

    Gene expression databases

    ArrayExpressiO55055.
    BgeeiO55055.
    CleanExiMM_TRDMT1.
    GenevestigatoriO55055.

    Structurei

    3D structure databases

    ProteinModelPortaliO55055.
    SMRiO55055. Positions 2-391.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 396393SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0270.
    HOGENOMiHOG000265038.
    HOVERGENiHBG051385.
    InParanoidiO55055.
    KOiK15336.
    OMAiFGVPYSR.
    OrthoDBiEOG7PZRZ4.
    PhylomeDBiO55055.
    TreeFamiTF300024.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR025813. DNA/tRNA_C5-MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O55055-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPLRVLELY SGIGGMHHAL RESHIPAHVV AAIDVNTVAN EVYKHNFPHT    50
    HLLSKTIEGI SLEDFDKLSF NMILMSPPCQ PFTRIGLQGD MTDPRTTSFL 100
    YILDILPRLQ KLPKYILLEN VKGFEVSSTR GLLIQTIEAC GFQYQEFLLS 150
    PSSLGIPNSR LRYFLIAKLQ SEPFPFQAPG QILMEFPKIV TVEPQKYAVV 200
    EESQPRVQRT GPRICAESSS TQSSGKDTIL FKLETVEERD RKHQQDSDLS 250
    VQMLKDFLED GDTDEYLLPP KLLLRYALLL DIVKPTSRRS MCFTKGYGSY 300
    IEGTGSVLQA AEDAQIENIY KSLPDLPPEE KIAKLSMLKL RYFTPKEIAN 350
    LQGFPPEFGF PEKTTVKQRY RLLGNSLNVH VVAKLLTVLC EGFGNASESC 400
    HKMPLILDSN SKILS 415
    Length:415
    Mass (Da):46,794
    Last modified:February 21, 2002 - v2
    Checksum:i14FEF78C0D2F8BFB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti164 – 1641F → S in AAC40130. (PubMed:9592134)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF012129 mRNA. Translation: AAC53529.1.
    AF045889 mRNA. Translation: AAC40130.1.
    CCDSiCCDS15695.1.
    RefSeqiNP_034197.3. NM_010067.4.
    UniGeneiMm.6979.

    Genome annotation databases

    EnsembliENSMUST00000124488; ENSMUSP00000114572; ENSMUSG00000026723.
    GeneIDi13434.
    KEGGimmu:13434.
    UCSCiuc008ika.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF012129 mRNA. Translation: AAC53529.1 .
    AF045889 mRNA. Translation: AAC40130.1 .
    CCDSi CCDS15695.1.
    RefSeqi NP_034197.3. NM_010067.4.
    UniGenei Mm.6979.

    3D structure databases

    ProteinModelPortali O55055.
    SMRi O55055. Positions 2-391.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei O55055.

    Proteomic databases

    PaxDbi O55055.
    PRIDEi O55055.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000124488 ; ENSMUSP00000114572 ; ENSMUSG00000026723 .
    GeneIDi 13434.
    KEGGi mmu:13434.
    UCSCi uc008ika.2. mouse.

    Organism-specific databases

    CTDi 1787.
    MGIi MGI:1274787. Trdmt1.

    Phylogenomic databases

    eggNOGi COG0270.
    HOGENOMi HOG000265038.
    HOVERGENi HBG051385.
    InParanoidi O55055.
    KOi K15336.
    OMAi FGVPYSR.
    OrthoDBi EOG7PZRZ4.
    PhylomeDBi O55055.
    TreeFami TF300024.

    Enzyme and pathway databases

    BRENDAi 2.1.1.37. 3474.

    Miscellaneous databases

    NextBioi 283863.
    PROi O55055.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O55055.
    Bgeei O55055.
    CleanExi MM_TRDMT1.
    Genevestigatori O55055.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR025813. DNA/tRNA_C5-MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A candidate mammalian DNA methyltransferase related to pmt1p of fission yeast."
      Yoder J.A., Bestor T.H.
      Hum. Mol. Genet. 7:279-284(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells."
      Okano M., Xie S., Li E.
      Nucleic Acids Res. 26:2536-2540(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Embryonic stem cell.

    Entry informationi

    Entry nameiTRDMT_MOUSE
    AccessioniPrimary (citable) accession number: O55055
    Secondary accession number(s): O35212
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: February 21, 2002
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3