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Protein

tRNA (cytosine(38)-C(5))-methyltransferase

Gene

Trdmt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).By similarity

Catalytic activityi

S-adenosyl-L-methionine + cytosine(38) in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(38) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei79 – 791PROSITE-ProRule annotation

GO - Molecular functioni

  • DNA binding Source: InterPro
  • RNA binding Source: UniProtKB-KW
  • tRNA methyltransferase activity Source: MGI

GO - Biological processi

  • DNA methylation Source: InterPro
  • response to amphetamine Source: Ensembl
  • tRNA methylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.37. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytosine(38)-C(5))-methyltransferase (EC:2.1.1.204)
Alternative name(s):
DNA (cytosine-5)-methyltransferase-like protein 2
Short name:
Dnmt2
DNA methyltransferase homolog MmuIIP
Short name:
DNA MTase homolog MmuIIP
Short name:
M.MmuIIP
Met-2
Gene namesi
Name:Trdmt1
Synonyms:Dnmt2, Met2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1274787. Trdmt1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415tRNA (cytosine(38)-C(5))-methyltransferasePRO_0000088041Add
BLAST

Proteomic databases

MaxQBiO55055.
PaxDbiO55055.
PRIDEiO55055.

PTM databases

PhosphoSiteiO55055.

Expressioni

Tissue specificityi

Highly expressed in thymus, testis, and at much lower levels in spleen, lung, brain, heart, kidney, liver, skeletal muscle and embryonic stem cells.1 Publication

Gene expression databases

BgeeiO55055.
CleanExiMM_TRDMT1.
ExpressionAtlasiO55055. baseline and differential.
GenevisibleiO55055. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000114572.

Structurei

3D structure databases

ProteinModelPortaliO55055.
SMRiO55055. Positions 2-391.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 396393SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0270.
HOGENOMiHOG000265038.
HOVERGENiHBG051385.
InParanoidiO55055.
KOiK15336.
OMAiPDSKRCC.
OrthoDBiEOG7PZRZ4.
PhylomeDBiO55055.
TreeFamiTF300024.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O55055-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPLRVLELY SGIGGMHHAL RESHIPAHVV AAIDVNTVAN EVYKHNFPHT
60 70 80 90 100
HLLSKTIEGI SLEDFDKLSF NMILMSPPCQ PFTRIGLQGD MTDPRTTSFL
110 120 130 140 150
YILDILPRLQ KLPKYILLEN VKGFEVSSTR GLLIQTIEAC GFQYQEFLLS
160 170 180 190 200
PSSLGIPNSR LRYFLIAKLQ SEPFPFQAPG QILMEFPKIV TVEPQKYAVV
210 220 230 240 250
EESQPRVQRT GPRICAESSS TQSSGKDTIL FKLETVEERD RKHQQDSDLS
260 270 280 290 300
VQMLKDFLED GDTDEYLLPP KLLLRYALLL DIVKPTSRRS MCFTKGYGSY
310 320 330 340 350
IEGTGSVLQA AEDAQIENIY KSLPDLPPEE KIAKLSMLKL RYFTPKEIAN
360 370 380 390 400
LQGFPPEFGF PEKTTVKQRY RLLGNSLNVH VVAKLLTVLC EGFGNASESC
410
HKMPLILDSN SKILS
Length:415
Mass (Da):46,794
Last modified:February 21, 2002 - v2
Checksum:i14FEF78C0D2F8BFB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1641F → S in AAC40130 (PubMed:9592134).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012129 mRNA. Translation: AAC53529.1.
AF045889 mRNA. Translation: AAC40130.1.
CCDSiCCDS15695.1.
RefSeqiNP_034197.3. NM_010067.4.
UniGeneiMm.6979.

Genome annotation databases

EnsembliENSMUST00000124488; ENSMUSP00000114572; ENSMUSG00000026723.
GeneIDi13434.
KEGGimmu:13434.
UCSCiuc008ika.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012129 mRNA. Translation: AAC53529.1.
AF045889 mRNA. Translation: AAC40130.1.
CCDSiCCDS15695.1.
RefSeqiNP_034197.3. NM_010067.4.
UniGeneiMm.6979.

3D structure databases

ProteinModelPortaliO55055.
SMRiO55055. Positions 2-391.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000114572.

PTM databases

PhosphoSiteiO55055.

Proteomic databases

MaxQBiO55055.
PaxDbiO55055.
PRIDEiO55055.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000124488; ENSMUSP00000114572; ENSMUSG00000026723.
GeneIDi13434.
KEGGimmu:13434.
UCSCiuc008ika.2. mouse.

Organism-specific databases

CTDi1787.
MGIiMGI:1274787. Trdmt1.

Phylogenomic databases

eggNOGiCOG0270.
HOGENOMiHOG000265038.
HOVERGENiHBG051385.
InParanoidiO55055.
KOiK15336.
OMAiPDSKRCC.
OrthoDBiEOG7PZRZ4.
PhylomeDBiO55055.
TreeFamiTF300024.

Enzyme and pathway databases

BRENDAi2.1.1.37. 3474.

Miscellaneous databases

NextBioi283863.
PROiO55055.
SOURCEiSearch...

Gene expression databases

BgeeiO55055.
CleanExiMM_TRDMT1.
ExpressionAtlasiO55055. baseline and differential.
GenevisibleiO55055. MM.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A candidate mammalian DNA methyltransferase related to pmt1p of fission yeast."
    Yoder J.A., Bestor T.H.
    Hum. Mol. Genet. 7:279-284(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells."
    Okano M., Xie S., Li E.
    Nucleic Acids Res. 26:2536-2540(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Embryonic stem cell.

Entry informationi

Entry nameiTRDMT_MOUSE
AccessioniPrimary (citable) accession number: O55055
Secondary accession number(s): O35212
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: February 21, 2002
Last modified: June 24, 2015
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.