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O55052

- MIF_MERUN

UniProt

O55052 - MIF_MERUN

Protein

Macrophage migration inhibitory factor

Gene

MIF

Organism
Meriones unguiculatus (Mongolian jird) (Mongolian gerbil)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
  1. Functioni

    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity By similarity.By similarity

    Catalytic activityi

    Keto-phenylpyruvate = enol-phenylpyruvate.
    L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Proton acceptor; via imino nitrogenBy similarity
    Binding sitei33 – 331SubstrateBy similarity
    Binding sitei65 – 651Substrate; via amide nitrogenBy similarity
    Binding sitei98 – 981SubstrateBy similarity

    GO - Molecular functioni

    1. dopachrome isomerase activity Source: UniProtKB
    2. phenylpyruvate tautomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. inflammatory response Source: UniProtKB-KW
    2. innate immune response Source: UniProtKB-KW
    3. metabolic process Source: GOC

    Keywords - Molecular functioni

    Cytokine, Isomerase

    Keywords - Biological processi

    Immunity, Inflammatory response, Innate immunity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Macrophage migration inhibitory factor (EC:5.3.2.1)
    Short name:
    MIF
    Alternative name(s):
    L-dopachrome isomerase
    L-dopachrome tautomerase (EC:5.3.3.12)
    Phenylpyruvate tautomerase
    Gene namesi
    Name:MIF
    OrganismiMeriones unguiculatus (Mongolian jird) (Mongolian gerbil)
    Taxonomic identifieri10047 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeGerbillinaeMeriones

    Subcellular locationi

    Secreted By similarity. Cytoplasm By similarity
    Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. extracellular space Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 115114Macrophage migration inhibitory factorPRO_0000158065Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei78 – 781N6-acetyllysine; alternateBy similarity
    Modified residuei78 – 781N6-succinyllysine; alternateBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiO55052.

    Interactioni

    Subunit structurei

    Homotrimer. Interacts with BNIPL By similarity. Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO55052.
    SMRiO55052. Positions 2-115.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MIF family.Curated

    Phylogenomic databases

    HOVERGENiHBG003240.

    Family and domain databases

    InterProiIPR001398. Macrophage_inhib_fac.
    IPR019829. Macrophage_inhib_fac_CS.
    IPR014347. Tautomerase/MIF_sf.
    [Graphical view]
    PANTHERiPTHR11954. PTHR11954. 1 hit.
    PfamiPF01187. MIF. 1 hit.
    [Graphical view]
    ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF55331. SSF55331. 1 hit.
    PROSITEiPS01158. MIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O55052-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPMFIVNTNV PRSSVPEGLL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF    50
    SGSSDPCALC SLHSIGKIGG AQNRTYSKLL CGLLADRLRI SPDRIYINYY 100
    DMNAANVGWN GSTFA 115
    Length:115
    Mass (Da):12,449
    Last modified:January 23, 2007 - v3
    Checksum:iF3DD3EB8F6F1597D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF045740 mRNA. Translation: AAC02629.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF045740 mRNA. Translation: AAC02629.1 .

    3D structure databases

    ProteinModelPortali O55052.
    SMRi O55052. Positions 2-115.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O55052.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG003240.

    Family and domain databases

    InterProi IPR001398. Macrophage_inhib_fac.
    IPR019829. Macrophage_inhib_fac_CS.
    IPR014347. Tautomerase/MIF_sf.
    [Graphical view ]
    PANTHERi PTHR11954. PTHR11954. 1 hit.
    Pfami PF01187. MIF. 1 hit.
    [Graphical view ]
    ProDomi PD004816. Macrophage_inhib_fac. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF55331. SSF55331. 1 hit.
    PROSITEi PS01158. MIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "MIF protein levels change during Brugia malayi infections in the gerbil."
      Marson A., Pastrana D., Raghavan N., Scott A.L.
      Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiMIF_MERUN
    AccessioniPrimary (citable) accession number: O55052
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 70 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3