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Protein

Rho guanine nucleotide exchange factor 7

Gene

Arhgef7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons (By similarity).By similarity

GO - Molecular functioni

  • guanyl-nucleotide exchange factor activity Source: RGD
  • protein kinase binding Source: RGD
  • Rho guanyl-nucleotide exchange factor activity Source: InterPro

GO - Biological processi

  • astrocyte cell migration Source: RGD
  • lamellipodium assembly Source: UniProtKB
  • positive regulation of apoptotic process Source: UniProtKB
  • regulation of Rho protein signal transduction Source: InterPro
  • small GTPase mediated signal transduction Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Neurogenesis

Enzyme and pathway databases

ReactomeiR-RNO-182971. EGFR downregulation.
R-RNO-193648. NRAGE signals death through JNK.
R-RNO-194840. Rho GTPase cycle.
R-RNO-3928664. Ephrin signaling.
R-RNO-416482. G alpha (12/13) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 7
Alternative name(s):
Beta-Pix
PAK-interacting exchange factor beta
Gene namesi
Name:Arhgef7
Synonyms:Pak3bp, Pixb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi620624. Arhgef7.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: UniProtKB-SubCell
  • cytoplasm Source: RGD
  • focal adhesion Source: RGD
  • growth cone Source: RGD
  • lamellipodium Source: UniProtKB
  • ruffle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 646646Rho guanine nucleotide exchange factor 7PRO_0000080923Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei7 – 71PhosphoserineCombined sources
Modified residuei71 – 711PhosphoserineCombined sources
Modified residuei79 – 791PhosphoserineCombined sources
Modified residuei340 – 3401PhosphoserineCombined sources
Modified residuei516 – 5161PhosphoserineCombined sources
Modified residuei560 – 5601PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated on Ser-516 by CaMK1; enhancement of GEF activity and downstream activation of RAC1. Phosphorylated by PTK2/FAK1; this promotes interaction with RAC1 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO55043.
PeptideAtlasiO55043.
PRIDEiO55043.

PTM databases

iPTMnetiO55043.
PhosphoSiteiO55043.

Expressioni

Gene expression databases

ExpressionAtlasiO55043. baseline and differential.
GenevisibleiO55043. RN.

Interactioni

Subunit structurei

Interacts with SCRIB; interaction is direct and may play a role in regulation of apoptosis (By similarity). Interacts with PAK kinases through the SH3 domain. Interacts with GIT1 and probably TGFB1I1. Interacts with ITCH and PARVB. Interacts with FRMPD4 (via N-terminus). Interacts with CaMK1. Interacts with PTK2/FAK1 and RAC1. Interacts with BIN2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
NAA10P412273EBI-3649585,EBI-747693From a different organism.
PAK2Q131778EBI-3649585,EBI-1045887From a different organism.

GO - Molecular functioni

  • protein kinase binding Source: RGD

Protein-protein interaction databases

BioGridi250377. 1 interaction.
DIPiDIP-41481N.
IntActiO55043. 8 interactions.
MINTiMINT-1536146.
STRINGi10116.ENSRNOP00000043761.

Structurei

Secondary structure

1
646
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 156Combined sources
Beta strandi32 – 387Combined sources
Beta strandi42 – 487Combined sources
Beta strandi51 – 566Combined sources
Helixi57 – 593Combined sources
Beta strandi60 – 623Combined sources
Helixi588 – 63649Combined sources
Beta strandi640 – 6456Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AK5X-ray1.85A/B5-66[»]
2DF6X-ray1.30A/B10-63[»]
2G6FX-ray0.92X10-63[»]
2P4RX-ray2.00A10-63[»]
2W6BX-ray2.80A588-638[»]
3L4FX-ray2.80A/B/C587-646[»]
3QJNX-ray2.71I/J/K/L/M/N/O/P640-646[»]
ProteinModelPortaliO55043.
SMRiO55043. Positions 6-65, 82-400.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO55043.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 6560SH3PROSITE-ProRule annotationAdd
BLAST
Domaini93 – 273181DHPROSITE-ProRule annotationAdd
BLAST
Domaini295 – 400106PHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG2070. Eukaryota.
ENOG410XNNP. LUCA.
GeneTreeiENSGT00760000118925.
HOGENOMiHOG000231381.
HOVERGENiHBG050569.
InParanoidiO55043.
KOiK13710.
PhylomeDBiO55043.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR032409. GEF_CC.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF16523. betaPIX_CC. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O55043-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDNANSQLV VRAKFNFQQT NEDELSFSKG DVIHVTRVEE GGWWEGTHNG
60 70 80 90 100
RTGWFPSNYV REIKPSEKPV SPKSGTLKSP PKGFDTTAIN KSYYNVVLQN
110 120 130 140 150
ILETEHEYSK ELQSVLSTYL WPLQTSEKLS SANTSYLMGN LEEISSFQQV
160 170 180 190 200
LVQSLEECTK SPEAQQRVGG CFLSLMPQMR TLYLAYCANH PSAVSVLTEH
210 220 230 240 250
SEDLGEFMET KGASSPGILV LTTGLSKPFM RLDKYPTLLK ELERHMEDYH
260 270 280 290 300
PDRQDIQKSM TAFKNLSAQC QEVRKRKELE LQILTEPIRS WEGDDIKTLG
310 320 330 340 350
SVTYMSQVTI QCAGSEEKNE RYLLLFPNLL LMLSASPRMS GFIYQGKLPT
360 370 380 390 400
TGMTITKLED SENHRNAFEI SGSMIERILV SCNNQQDLHE WVEHLQRQTK
410 420 430 440 450
VTSVSNPTIK PHSVPSHTLP SHPLTPSSKH ADSKPVALTP AYHTLPHPSH
460 470 480 490 500
HGTPHTTISW GPLEPPKTPK PWSLSCLRPA PPLRPSAALC YKEDLSRSPK
510 520 530 540 550
TMKKLLPKRK PERKPSDEEF AVRKSTAALE EDAQILKVIE AYCTSAKTRQ
560 570 580 590 600
TLNSSSRKES APQVLLPEEE KIIVEETKSN GQTVIEEKSL VDTVYALKDE
610 620 630 640
VQELRQDNKK MKKSLEEEQR ARKDLEKLVR KVLKNMNDPA WDETNL
Length:646
Mass (Da):73,140
Last modified:June 1, 1998 - v1
Checksum:i17CDE7DD96ADEA53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044673 mRNA. Translation: AAC39971.1.
RefSeqiNP_001106994.1. NM_001113522.1.
UniGeneiRn.146763.

Genome annotation databases

EnsembliENSRNOT00000017947; ENSRNOP00000017947; ENSRNOG00000012934.
GeneIDi114559.
KEGGirno:114559.
UCSCiRGD:620624. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044673 mRNA. Translation: AAC39971.1.
RefSeqiNP_001106994.1. NM_001113522.1.
UniGeneiRn.146763.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AK5X-ray1.85A/B5-66[»]
2DF6X-ray1.30A/B10-63[»]
2G6FX-ray0.92X10-63[»]
2P4RX-ray2.00A10-63[»]
2W6BX-ray2.80A588-638[»]
3L4FX-ray2.80A/B/C587-646[»]
3QJNX-ray2.71I/J/K/L/M/N/O/P640-646[»]
ProteinModelPortaliO55043.
SMRiO55043. Positions 6-65, 82-400.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250377. 1 interaction.
DIPiDIP-41481N.
IntActiO55043. 8 interactions.
MINTiMINT-1536146.
STRINGi10116.ENSRNOP00000043761.

PTM databases

iPTMnetiO55043.
PhosphoSiteiO55043.

Proteomic databases

PaxDbiO55043.
PeptideAtlasiO55043.
PRIDEiO55043.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017947; ENSRNOP00000017947; ENSRNOG00000012934.
GeneIDi114559.
KEGGirno:114559.
UCSCiRGD:620624. rat.

Organism-specific databases

CTDi8874.
RGDi620624. Arhgef7.

Phylogenomic databases

eggNOGiKOG2070. Eukaryota.
ENOG410XNNP. LUCA.
GeneTreeiENSGT00760000118925.
HOGENOMiHOG000231381.
HOVERGENiHBG050569.
InParanoidiO55043.
KOiK13710.
PhylomeDBiO55043.

Enzyme and pathway databases

ReactomeiR-RNO-182971. EGFR downregulation.
R-RNO-193648. NRAGE signals death through JNK.
R-RNO-194840. Rho GTPase cycle.
R-RNO-3928664. Ephrin signaling.
R-RNO-416482. G alpha (12/13) signalling events.

Miscellaneous databases

EvolutionaryTraceiO55043.
PROiO55043.

Gene expression databases

ExpressionAtlasiO55043. baseline and differential.
GenevisibleiO55043. RN.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR032409. GEF_CC.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF16523. betaPIX_CC. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "PAK kinases are directly coupled to the PIX family of nucleotide exchange factors."
    Manser E., Loo T.-H., Koh C.-G., Zhao Z.-S., Chen X.-Q., Tan L., Tan I., Leung T., Lim L.
    Mol. Cell 1:183-192(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The GIT family of ADP-ribosylation factor GTPase-activating proteins. Functional diversity of GIT2 through alternative splicing."
    Premont R.T., Claing A., Vitale N., Perry S.J., Lefkowitz R.J.
    J. Biol. Chem. 275:22373-22380(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GIT1.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7; SER-71; SER-79; SER-340; SER-516 AND SER-560, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 5-66.
  5. "Crystal structure of the SH3 domain of betaPIX in complex with a high affinity peptide from PAK2."
    Hoelz A., Janz J.M., Lawrie S.D., Corwin B., Lee A., Sakmar T.P.
    J. Mol. Biol. 358:509-522(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (0.92 ANGSTROMS) OF 10-63 IN COMPLEX WITH PAK2, SUBUNIT.
  6. "A novel interaction between atrophin-interacting protein 4 and beta-p21-activated kinase-interactive exchange factor is mediated by an SH3 domain."
    Janz J.M., Sakmar T.P., Min K.C.
    J. Biol. Chem. 282:28893-28903(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 10-63 IN COMPLEX WITH ITCH.

Entry informationi

Entry nameiARHG7_RAT
AccessioniPrimary (citable) accession number: O55043
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: July 6, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.