O55042 (SYUA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-synuclein

Alternative name(s):
Non-A beta component of AD amyloid
Non-A4 component of amyloid precursor
Short name=NACP

Gene names

Name:	Snca
Synonyms:	Syn

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in the regulation of dopamine release and transport.
Subunit structure	Interacts with UCHL1 By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Phosphorylated, predominantly on serine residues. Phosphorylated on Tyr-125 upon osmotic stress By similarity. Ref.9 Ubiquitinated. The predominant conjugate is the diubiquitinated form By similarity. Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure By similarity.
Sequence similarities	Belongs to the synuclein family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Copper Metal-binding
PTM	Acetylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	activation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from electronic annotation. Source: Compara adult locomotory behavior Inferred from genetic interaction PubMed 18333965. Source: MGI aging Inferred from electronic annotation. Source: Compara behavioral response to cocaine Inferred from electronic annotation. Source: Compara cellular response to copper ion Inferred from electronic annotation. Source: Compara cellular response to fibroblast growth factor stimulus Inferred from electronic annotation. Source: Compara cellular response to oxidative stress Inferred from mutant phenotype PubMed 16298531. Source: MGI dopamine biosynthetic process Inferred from mutant phenotype PubMed 10707987. Source: MGI fatty acid metabolic process Inferred from mutant phenotype PubMed 14507911PubMed 17250686. Source: MGI long-term synaptic potentiation Inferred from mutant phenotype PubMed 17689254. Source: MGI microglial cell activation Inferred from mutant phenotype PubMed 17035541. Source: MGI mitochondrial ATP synthesis coupled electron transport Inferred from mutant phenotype PubMed 16260631. Source: MGI mitochondrial membrane organization Inferred from mutant phenotype PubMed 16260631. Source: MGI negative regulation of dopamine metabolic process Inferred from electronic annotation. Source: Compara negative regulation of dopamine uptake involved in synaptic transmission Inferred from electronic annotation. Source: Compara negative regulation of exocytosis Inferred from electronic annotation. Source: Compara negative regulation of histone acetylation Inferred from electronic annotation. Source: Compara negative regulation of microtubule polymerization Inferred from electronic annotation. Source: Compara negative regulation of monooxygenase activity Inferred from electronic annotation. Source: Compara negative regulation of neuron apoptotic process Inferred from genetic interaction PubMed 16269331. Source: MGI negative regulation of norepinephrine uptake Inferred from electronic annotation. Source: Compara negative regulation of platelet-derived growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of protein phosphorylation Inferred from electronic annotation. Source: Compara negative regulation of serotonin uptake Inferred from electronic annotation. Source: Compara negative regulation of thrombin receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of transporter activity Inferred from electronic annotation. Source: Compara neutral lipid metabolic process Inferred from mutant phenotype PubMed 17250686. Source: MGI phospholipid metabolic process Inferred from mutant phenotype PubMed 15938614PubMed 16260631PubMed 16734431. Source: MGI positive regulation of endocytosis Inferred from electronic annotation. Source: Compara positive regulation of inositol phosphate biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of neurotransmitter secretion Inferred from direct assay PubMed 15510220. Source: MGI positive regulation of peptidyl-serine phosphorylation Inferred from mutant phenotype PubMed 21127069. Source: BHF-UCL positive regulation of protein serine/threonine kinase activity Inferred from electronic annotation. Source: Compara positive regulation of receptor recycling Inferred from electronic annotation. Source: Compara positive regulation of release of sequestered calcium ion into cytosol Inferred from electronic annotation. Source: Compara protein destabilization Inferred from electronic annotation. Source: Compara receptor internalization Inferred from electronic annotation. Source: Compara regulation of acyl-CoA biosynthetic process Inferred from direct assay PubMed 16734431. Source: MGI regulation of dopamine secretion Inferred from genetic interaction PubMed 18333965. Source: MGI regulation of excitatory postsynaptic membrane potential Inferred from mutant phenotype PubMed 17610578. Source: MGI regulation of glutamate secretion Inferred from mutant phenotype PubMed 17689254. Source: MGI regulation of locomotion Inferred from mutant phenotype PubMed 10707987. Source: MGI regulation of long-term neuronal synaptic plasticity Inferred from mutant phenotype PubMed 15510220. Source: MGI regulation of macrophage activation Inferred from mutant phenotype PubMed 17035541. Source: MGI regulation of phospholipase activity Inferred from electronic annotation. Source: Compara response to drug Inferred from mutant phenotype PubMed 10707987. Source: MGI response to interferon-gamma Inferred from electronic annotation. Source: Compara response to interleukin-1 Inferred from electronic annotation. Source: Compara response to iron(II) ion Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from electronic annotation. Source: Compara response to magnesium ion Inferred from electronic annotation. Source: Compara synapse organization Inferred from genetic interaction PubMed 20974939. Source: MGI synaptic transmission, dopaminergic Inferred from mutant phenotype PubMed 10707987PubMed 12388586. Source: MGI synaptic vesicle endocytosis Inferred from mutant phenotype PubMed 18980610. Source: UniProtKB
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara actin cytoskeleton Inferred from electronic annotation. Source: Compara cell cortex Inferred from electronic annotation. Source: Compara cytoskeleton Inferred from direct assay PubMed 17077307. Source: MGI cytosol Inferred from direct assay PubMed 18980610. Source: UniProtKB fibril Inferred from electronic annotation. Source: Compara growth cone Inferred from electronic annotation. Source: Compara inclusion body Inferred from electronic annotation. Source: Compara mitochondrion Inferred from electronic annotation. Source: Compara nuclear outer membrane Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay PubMed 16959795. Source: UniProtKB perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara plasma membrane Inferred from direct assay PubMed 17475220. Source: MGI platelet alpha granule membrane Inferred from electronic annotation. Source: Compara ribosome Inferred from electronic annotation. Source: Compara rough endoplasmic reticulum Inferred from electronic annotation. Source: Compara synapse Inferred from direct assay PubMed 10964942. Source: MGI synaptic vesicle Inferred from electronic annotation. Source: Compara terminal bouton Inferred from electronic annotation. Source: Compara
Molecular_function	arachidonic acid binding Inferred from direct assay PubMed 16734431. Source: MGI calcium ion binding Inferred from electronic annotation. Source: Compara copper ion binding Inferred from sequence or structural similarity. Source: UniProtKB cysteine-type endopeptidase inhibitor activity involved in apoptotic process Inferred from electronic annotation. Source: Compara ferrous iron binding Inferred from electronic annotation. Source: Compara histone binding Inferred from direct assay PubMed 16959795. Source: UniProtKB identical protein binding Inferred from direct assay PubMed 10978144. Source: MGI magnesium ion binding Inferred from electronic annotation. Source: Compara oxidoreductase activity Inferred from electronic annotation. Source: Compara phospholipid binding Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O55042-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O55042-2)

The sequence of this isoform differs from the canonical sequence as follows:
     103-121: GEEGYPQEGILEDMPVDPG → VWLPVLCSVITLDTMSLHA
     122-140: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 140	140	Alpha-synuclein		PRO_0000184026
Regions
	Repeat	20 – 30	11	1
	Repeat	31 – 41	11	2
	Repeat	42 – 56	15	3; approximate
	Repeat	57 – 67	11	4
	Region	20 – 67	48	4 X 11 AA tandem repeats of [EGS]-K-T-K-[EQ]-[GQ]-V-X(4)
Sites
	Metal binding	2	1	Copper By similarity
	Metal binding	50	1	Copper By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	125	1	Phosphotyrosine; by FYN By similarity
	Modified residue	129	1	Phosphoserine; by PLK2 Ref.9
Natural variations
	Alternative sequence	103 – 121	19	GEEGY…PVDPG → VWLPVLCSVITLDTMSLHA in isoform 2.		VSP_025018
	Alternative sequence	122 – 140	19	Missing in isoform 2.		VSP_025019
Experimental info
	Sequence conflict	58	1	K → T in AAC00521. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 1FFD19D7E15E636C	FASTA	140	14,485
	10 20 30 40 50 60 MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK 70 80 90 100 110 120 EQVTNVGGAV VTGVTAVAQK TVEGAGNIAA ATGFVKKDQM GKGEEGYPQE GILEDMPVDP 130 140 GSEAYEMPSE EGYQDYEPEA
	Isoform 2 [UniParc]. Checksum: 03CAFC4944C78D63	FASTA	121	12,345
	10 20 30 40 50 60 MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK 70 80 90 100 110 120 EQVTNVGGAV VTGVTAVAQK TVEGAGNIAA ATGFVKKDQM GKVWLPVLCS VITLDTMSLH A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression pattern of synucleins (non-Abeta component of Alzheimer's disease amyloid precursor protein/alpha-synuclein) during murine brain development." Hsu L.J., Mallory M., Xia Y., Veinbergs I., Hashimoto M., Yoshimoto M., Thal L.J., Saitoh T., Masliah E. J. Neurochem. 71:338-344(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The cDNA cloning and ontogeny of mouse alpha-synuclein." Hong L., Ko H.W., Gwag B.J., Joe E., Lee S., Kim Y.T., Suh Y.-H. NeuroReport 9:1239-1243(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: ICR.
[3]	"Genomic cloning of the mouse alpha-synuclein and analysis of the promoter." Fog J.U., Kallunki P. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1). Strain: C57BL/6J and Sv129/Ola. Tissue: Brain.
[4]	"Human and mouse alpha-synuclein genes: comparative genomic sequence analysis and identification of a novel gene regulatory element." Touchman J.W., Dehejia A., Chiba-Falek O., Cabin D.E., Schwartz J.R., Orrison B.M., Polymeropoulos M.H., Nussbaum R.L. Genome Res. 11:78-86(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[5]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J and NOD. Tissue: Liver and Spleen.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
[7]	"Role of alpha-synuclein in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced parkinsonism in mice." Schlueter O.M., Fornai F., Alessandri M.G., Takamori S., Geppert M., Jahn R., Suedhof T.C. Neuroscience 118:985-1002(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-54. Strain: 129/SvJ.
[8]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 61-96, MASS SPECTROMETRY. Tissue: Hippocampus.
[9]	"Polo-like kinase 2 (PLK2) phosphorylates alpha-synuclein at serine 129 in central nervous system." Inglis K.J., Chereau D., Brigham E.F., Chiou S.S., Schobel S., Frigon N.L., Yu M., Caccavello R.J., Nelson S., Motter R., Wright S., Chian D., Santiago P., Soriano F., Ramos C., Powell K., Goldstein J.M., Babcock M. , Yednock T., Bard F., Basi G.S., Sham H., Chilcote T.J., McConlogue L., Griswold-Prenner I., Anderson J.P. J. Biol. Chem. 284:2598-2602(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-129.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF044672 mRNA. Translation: AAC00521.1. AF033261 mRNA. Translation: AAD11254.1. AF179273 mRNA. Translation: AAD56908.1. AF179272 , AF179268, AF179269, AF179270, AF179271 Genomic DNA. Translation: AAD56907.1. AF163865 Genomic DNA. Translation: AAG30304.1. AK014472 mRNA. Translation: BAB29375.1. AK156316 mRNA. Translation: BAE33670.1. BC046764 mRNA. Translation: AAH46764.1. AF277451 Genomic DNA. Translation: AAG44833.1.
IPI	IPI00115157. IPI00845803.
RefSeq	NP_001035916.1. NM_001042451.1. NP_033247.1. NM_009221.2.
UniGene	Mm.17484.
3D structure databases
ProteinModelPortal	O55042.
SMR	O55042. Positions 1-140.
ModBase	Search...
Protein-protein interaction databases
IntAct	O55042. 2 interactions.
MINT	MINT-2736846.
PTM databases
PhosphoSite	O55042.
Proteomic databases
PaxDb	O55042.
PRIDE	O55042.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000114268; ENSMUSP00000109907; ENSMUSG00000025889. ENSMUST00000163779; ENSMUSP00000126067; ENSMUSG00000025889.
GeneID	20617.
KEGG	mmu:20617.
UCSC	uc009cdn.1. mouse. uc009cdp.1. mouse.
Organism-specific databases
CTD	6622.
MGI	MGI:1277151. Snca.
Phylogenomic databases
eggNOG	NOG44393.
GeneTree	ENSGT00390000016161.
HOGENOM	HOG000008691.
HOVERGEN	HBG000481.
InParanoid	O55042.
KO	K04528.
OMA	MPPEEEY.
OrthoDB	EOG4FFD3B.
Gene expression databases
Bgee	O55042.
CleanEx	MM_SNCA.
Genevestigator	O55042.
GermOnline	ENSMUSG00000025889. Mus musculus.
Family and domain databases
Gene3D	1.10.287.700. 1 hit.
InterPro	IPR001058. Synuclein. IPR002460. Synuclein_alpha. [Graphical view]
PANTHER	PTHR13820. PTHR13820. 1 hit.
Pfam	PF01387. Synuclein. 1 hit. [Graphical view]
PRINTS	PR01212. ASYNUCLEIN. PR01211. SYNUCLEIN.
ProtoNet	Search...
Other
NextBio	298995.
PMAP-CutDB	O55042.
SOURCE	Search...

Entry information

Entry name

SYUA_MOUSE

Accession

Primary (citable) accession number: O55042
Secondary accession number(s): Q3U130 , Q9CXF8, Q9EQC3, Q9QUR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	April 27, 2001
Last modified:	May 1, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program