O55042 (SYUA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-synuclein

Alternative name(s):
Non-A beta component of AD amyloid
Non-A4 component of amyloid precursor
Short name=NACP

Gene names

Name:	Snca
Synonyms:	Syn

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in the regulation of dopamine release and transport.
Subunit structure	Interacts with UCHL1 By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Phosphorylated, predominantly on serine residues. Phosphorylated on Tyr-125 upon osmotic stress By similarity. Ref.9 Ubiquitinated. The predominant conjugate is the diubiquitinated form By similarity.
Sequence similarities	Belongs to the synuclein family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Repeat
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	adult locomotory behavior Inferred from genetic interaction. Source: MGI cellular response to oxidative stress Inferred from mutant phenotype. Source: MGI dopamine biosynthetic process Inferred from mutant phenotype. Source: MGI fatty acid metabolic process Inferred from mutant phenotype. Source: MGI long-term synaptic potentiation Inferred from mutant phenotype. Source: MGI microglial cell activation Inferred from mutant phenotype. Source: MGI mitochondrial ATP synthesis coupled electron transport Inferred from mutant phenotype. Source: MGI mitochondrial membrane organization Inferred from mutant phenotype. Source: MGI negative regulation of neuron apoptosis Inferred from genetic interaction. Source: MGI neutral lipid metabolic process Inferred from mutant phenotype. Source: MGI phospholipid metabolic process Inferred from mutant phenotype. Source: MGI positive regulation of neurotransmitter secretion Inferred from direct assay. Source: MGI positive regulation of peptidyl-serine phosphorylation Inferred from mutant phenotype. Source: BHF-UCL regulation of acyl-CoA biosynthetic process Inferred from direct assay. Source: MGI regulation of dopamine secretion Inferred from genetic interaction. Source: MGI regulation of excitatory postsynaptic membrane potential Inferred from mutant phenotype. Source: MGI regulation of glutamate secretion Inferred from mutant phenotype. Source: MGI regulation of locomotion Inferred from mutant phenotype. Source: MGI regulation of long-term neuronal synaptic plasticity Inferred from mutant phenotype. Source: MGI regulation of macrophage activation Inferred from mutant phenotype. Source: MGI response to drug Inferred from mutant phenotype. Source: MGI synaptic transmission, dopaminergic Inferred from mutant phenotype. Source: MGI synaptic vesicle endocytosis Inferred from mutant phenotype. Source: UniProtKB
Cellular component	cytosol Inferred from direct assay. Source: UniProtKB mitochondrion Inferred from mutant phenotype. Source: GOC nucleus Inferred from direct assay. Source: UniProtKB plasma membrane Inferred from direct assay. Source: MGI synaptosome Inferred from direct assay. Source: MGI
Molecular function	arachidonic acid binding Inferred from direct assay. Source: MGI histone binding Inferred from direct assay. Source: UniProtKB identical protein binding Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O55042-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O55042-2)

The sequence of this isoform differs from the canonical sequence as follows:
     103-121: GEEGYPQEGILEDMPVDPG → VWLPVLCSVITLDTMSLHA
     122-140: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 140	140	Alpha-synuclein		PRO_0000184026
Regions
	Repeat	20 – 30	11	1
	Repeat	31 – 41	11	2
	Repeat	42 – 56	15	3; approximate
	Repeat	57 – 67	11	4
	Region	20 – 67	48	4 X 11 AA tandem repeats of [EGS]-K-T-K-[EQ]-[GQ]-V-X(4)
Amino acid modifications
	Modified residue	125	1	Phosphotyrosine By similarity
	Modified residue	125	1	Phosphotyrosine; by FYN By similarity
	Modified residue	129	1	Phosphoserine; by PLK2 Ref.9
Natural variations
	Alternative sequence	103 – 121	19	GEEGY…PVDPG → VWLPVLCSVITLDTMSLHA in isoform 2.		VSP_025018
	Alternative sequence	122 – 140	19	Missing in isoform 2.		VSP_025019
Experimental info
	Sequence conflict	58	1	K → T in AAC00521. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 1FFD19D7E15E636C	FASTA	140	14,485
	10 20 30 40 50 60 MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK 70 80 90 100 110 120 EQVTNVGGAV VTGVTAVAQK TVEGAGNIAA ATGFVKKDQM GKGEEGYPQE GILEDMPVDP 130 140 GSEAYEMPSE EGYQDYEPEA
	Isoform 2 [UniParc]. Checksum: 03CAFC4944C78D63	FASTA	121	12,345
	10 20 30 40 50 60 MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK 70 80 90 100 110 120 EQVTNVGGAV VTGVTAVAQK TVEGAGNIAA ATGFVKKDQM GKVWLPVLCS VITLDTMSLH A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression pattern of synucleins (non-Abeta component of Alzheimer's disease amyloid precursor protein/alpha-synuclein) during murine brain development." Hsu L.J., Mallory M., Xia Y., Veinbergs I., Hashimoto M., Yoshimoto M., Thal L.J., Saitoh T., Masliah E. J. Neurochem. 71:338-344(1998) [PubMed: 9648883] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The cDNA cloning and ontogeny of mouse alpha-synuclein." Hong L., Ko H.W., Gwag B.J., Joe E., Lee S., Kim Y.T., Suh Y.-H. NeuroReport 9:1239-1243(1998) [PubMed: 9601701] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: ICR.
[3]	"Genomic cloning of the mouse alpha-synuclein and analysis of the promoter." Fog J.U., Kallunki P. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1). Strain: C57BL/6J and Sv129/Ola. Tissue: Brain.
[4]	"Human and mouse alpha-synuclein genes: comparative genomic sequence analysis and identification of a novel gene regulatory element." Touchman J.W., Dehejia A., Chiba-Falek O., Cabin D.E., Schwartz J.R., Orrison B.M., Polymeropoulos M.H., Nussbaum R.L. Genome Res. 11:78-86(2001) [PubMed: 11156617] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[5]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J and NOD. Tissue: Liver and Spleen.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
[7]	"Role of alpha-synuclein in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced parkinsonism in mice." Schlueter O.M., Fornai F., Alessandri M.G., Takamori S., Geppert M., Jahn R., Suedhof T.C. Neuroscience 118:985-1002(2003) [PubMed: 12732244] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-54. Strain: 129/SvJ.
[8]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 61-96, MASS SPECTROMETRY. Tissue: Hippocampus.
[9]	"Polo-like kinase 2 (PLK2) phosphorylates alpha-synuclein at serine 129 in central nervous system." Inglis K.J., Chereau D., Brigham E.F., Chiou S.S., Schobel S., Frigon N.L., Yu M., Caccavello R.J., Nelson S., Motter R., Wright S., Chian D., Santiago P., Soriano F., Ramos C., Powell K., Goldstein J.M., Babcock M. , Yednock T., Bard F., Basi G.S., Sham H., Chilcote T.J., McConlogue L., Griswold-Prenner I., Anderson J.P. J. Biol. Chem. 284:2598-2602(2009) [PubMed: 19004816] [Abstract] Cited for: PHOSPHORYLATION AT SER-129.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF044672 mRNA. Translation: AAC00521.1. AF033261 mRNA. Translation: AAD11254.1. AF179273 mRNA. Translation: AAD56908.1. AF179272 , AF179268, AF179269, AF179270, AF179271 Genomic DNA. Translation: AAD56907.1. AF163865 Genomic DNA. Translation: AAG30304.1. AK014472 mRNA. Translation: BAB29375.1. AK156316 mRNA. Translation: BAE33670.1. BC046764 mRNA. Translation: AAH46764.1. AF277451 Genomic DNA. Translation: AAG44833.1.
IPI	IPI00115157. IPI00845803.
RefSeq	NP_001035916.1. NM_001042451.1. NP_033247.1. NM_009221.2.
UniGene	Mm.17484.
3D structure databases
ProteinModelPortal	O55042.
SMR	O55042. Positions 1-140.
ModBase	Search...
Protein-protein interaction databases
IntAct	O55042. 1 interaction.
MINT	MINT-2736846.
STRING	O55042.
PTM databases
PhosphoSite	O55042.
Proteomic databases
PRIDE	O55042.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000114268; ENSMUSP00000109907; ENSMUSG00000025889. ENSMUST00000163779; ENSMUSP00000126067; ENSMUSG00000025889.
GeneID	20617.
KEGG	mmu:20617.
UCSC	uc009cdn.1. mouse. uc009cdp.1. mouse.
Organism-specific databases
CTD	6622.
MGI	MGI:1277151. Snca.
Phylogenomic databases
eggNOG	roNOG16930.
GeneTree	ENSGT00390000016161.
HOGENOM	HBG715579.
HOVERGEN	HBG000481.
InParanoid	O55042.
OMA	PENEAYE.
OrthoDB	EOG4FFD3B.
PhylomeDB	O55042.
Gene expression databases
ArrayExpress	O55042.
Bgee	O55042.
CleanEx	MM_SNCA.
Genevestigator	O55042.
GermOnline	ENSMUSG00000025889. Mus musculus.
Family and domain databases
InterPro	IPR001058. Synuclein. IPR002460. Synuclein_alpha. [Graphical view]
Gene3D	G3DSA:1.10.287.700. Synuclein. 1 hit.
KO	K04528.
PANTHER	PTHR13820. Synuclein. 1 hit.
Pfam	PF01387. Synuclein. 1 hit. [Graphical view]
PRINTS	PR01212. ASYNUCLEIN. PR01211. SYNUCLEIN.
ProtoNet	Search...
Other
NextBio	298995.
PMAP-CutDB	O55042.
SOURCE	Search...

Entry information

Entry name

SYUA_MOUSE

Accession

Primary (citable) accession number: O55042
Secondary accession number(s): Q3U130 , Q9CXF8, Q9EQC3, Q9QUR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	April 27, 2001
Last modified:	December 14, 2011
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program