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Protein

Sperm-associated antigen 4 protein

Gene

Spag4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail.

GO - Molecular functioni

  • identical protein binding Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm-associated antigen 4 protein
Alternative name(s):
Outer dense fiber-associated protein SPAG4
SUN domain-containing protein 4
Gene namesi
Name:Spag4
Synonyms:Sun4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620151. Spag4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei137 – 15923HelicalSequence analysisAdd
BLAST
Transmembranei166 – 18823HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-KW
  • integral component of membrane Source: UniProtKB-KW
  • microtubule Source: RGD
  • motile cilium Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Sperm-associated antigen 4 proteinPRO_0000218918Add
BLAST

Proteomic databases

PaxDbiO55034.
PRIDEiO55034.

Expressioni

Tissue specificityi

Testis specific. Exclusively expressed in spermatids.

Developmental stagei

Exclusively expressed in spermatids. Not present in mature sperm. Localized with both the manchette and the axoneme in step 10-11 spermatids. Detected on manchette microtubules of step 12 spermatids. Associates with the tail in steps 18-19 spermatids and epididymal sperm.1 Publication

Interactioni

Subunit structurei

Homodimer. Interacts with ODF1. May associate with microtubules.1 Publication

GO - Molecular functioni

  • identical protein binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000058827.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini267 – 428162SUNPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili204 – 24138Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 SUN domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2687. Eukaryota.
ENOG410YM6S. LUCA.
HOGENOMiHOG000246956.
HOVERGENiHBG079205.
InParanoidiO55034.
PhylomeDBiO55034.

Family and domain databases

InterProiIPR027776. SPAG4/SUN4.
IPR012919. SUN_dom.
[Graphical view]
PANTHERiPTHR12911:SF16. PTHR12911:SF16. 1 hit.
PfamiPF07738. Sad1_UNC. 1 hit.
[Graphical view]
PROSITEiPS51469. SUN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O55034-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRNPRPGSA ASSHNHTPNF YSENSNSSHS ATSGDSNGRR SAGPELGEPD
60 70 80 90 100
GRMARGSSCG EPALSSGVPG GDTWAGSSRP KLAPRSHNGQ TACGAATVRG
110 120 130 140 150
GASEPSGSPA VLEEQLNLLP ILDLRQEMPP PPVSKSFLSL FFQVLSVFLS
160 170 180 190 200
LVADGLVCVY REICSIRFLF TAVSLLSIFL AALWWGLLYL IPPLENEPKE
210 220 230 240 250
MLTLSQYHHR VHSQGQQLQQ LQAELSKLHK EVTSVRAAHS ERVAKLVFQR
260 270 280 290 300
LNEDFVRKPD YALSSVGASI DLEKTSSDYE DRNTAYFWNR LSFWNYARPP
310 320 330 340 350
SVILEPDVFP GNCWAFEGEQ GQVVIRLPGH VQLSDITLQH PPPTVAHTGG
360 370 380 390 400
ASSAPRDFAV FGLQADDDET EVFLGKFIFE VQKSEIQTFH LQNDPPSAFP
410 420 430 440
KVKIQILSNW GHPRFTCLYR VRAHGVRISE SAEDNAMGVT GGPH
Length:444
Mass (Da):48,693
Last modified:May 1, 2000 - v2
Checksum:iDA05493E8F87C46B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043345 mRNA. Translation: AAC32053.2.
RefSeqiNP_113980.1. NM_031792.1.
UniGeneiRn.28620.

Genome annotation databases

GeneIDi83623.
KEGGirno:83623.
UCSCiRGD:620151. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043345 mRNA. Translation: AAC32053.2.
RefSeqiNP_113980.1. NM_031792.1.
UniGeneiRn.28620.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000058827.

Proteomic databases

PaxDbiO55034.
PRIDEiO55034.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83623.
KEGGirno:83623.
UCSCiRGD:620151. rat.

Organism-specific databases

CTDi6676.
RGDi620151. Spag4.

Phylogenomic databases

eggNOGiKOG2687. Eukaryota.
ENOG410YM6S. LUCA.
HOGENOMiHOG000246956.
HOVERGENiHBG079205.
InParanoidiO55034.
PhylomeDBiO55034.

Miscellaneous databases

PROiO55034.

Family and domain databases

InterProiIPR027776. SPAG4/SUN4.
IPR012919. SUN_dom.
[Graphical view]
PANTHERiPTHR12911:SF16. PTHR12911:SF16. 1 hit.
PfamiPF07738. Sad1_UNC. 1 hit.
[Graphical view]
PROSITEiPS51469. SUN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Spag4, a novel sperm protein, binds outer dense-fiber protein Odf1 and localizes to microtubules of manchette and axoneme."
    Shao X., Tarnasky H.A., Lee J.P., Oko R., van der Hoorn F.A.
    Dev. Biol. 211:109-123(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, DEVELOPMENTAL STAGE, HOMODIMERIZATION, INTERACTION WITH ODF1.
    Tissue: Testis.

Entry informationi

Entry nameiSPAG4_RAT
AccessioniPrimary (citable) accession number: O55034
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Although transmembrane domains are strongly predicted, they may rather represent hydrophobic globular domains associated with microtubules.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.