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Protein

Cytoplasmic protein NCK2

Gene

Nck2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling (By similarity).By similarity

GO - Molecular functioni

  • protein complex binding Source: MGI
  • scaffold protein binding Source: BHF-UCL

GO - Biological processi

  • actin filament organization Source: MGI
  • cell migration Source: MGI
  • dendritic spine development Source: MGI
  • ephrin receptor signaling pathway Source: MGI
  • immunological synapse formation Source: MGI
  • lamellipodium assembly Source: MGI
  • negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation Source: ParkinsonsUK-UCL
  • negative regulation of peptidyl-serine phosphorylation Source: MGI
  • negative regulation of PERK-mediated unfolded protein response Source: ParkinsonsUK-UCL
  • negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
  • positive regulation of actin filament polymerization Source: MGI
  • positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
  • positive regulation of T cell proliferation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of translation in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Biological processi

Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-186763. Downstream signal transduction.
R-MMU-373753. Nephrin interactions.
R-MMU-3928664. Ephrin signaling.
R-MMU-4420097. VEGFA-VEGFR2 Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic protein NCK2
Alternative name(s):
Growth factor receptor-bound protein 4
NCK adaptor protein 2
Short name:
Nck-2
SH2/SH3 adaptor protein NCK-beta
Gene namesi
Name:Nck2
Synonyms:Grb4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1306821. Nck2.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: MGI
  • neuronal postsynaptic density Source: MGI
  • synapse Source: MGI
  • vesicle membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 380379Cytoplasmic protein NCK2PRO_0000415287Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity
Modified residuei90 – 901PhosphoserineCombined sources
Modified residuei94 – 941PhosphoserineCombined sources
Modified residuei110 – 1101PhosphotyrosineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO55033.
MaxQBiO55033.
PaxDbiO55033.
PRIDEiO55033.

PTM databases

iPTMnetiO55033.
PhosphoSiteiO55033.

Expressioni

Gene expression databases

BgeeiO55033.
ExpressionAtlasiO55033. baseline and differential.
GenevisibleiO55033. MM.

Interactioni

Subunit structurei

Interacts with DOCK1, LIMS1 and TGFB1I1. Part of a complex containing PPP1R15B, PP1 and NCK2. Interacts with FASLG. Interacts with AXL. Interacts with PAK1, PKN2 and SOS1. Interacts (via SH2 domain) with EGFR. Interacts (via SH2 domain) with DDR1 (By similarity).By similarity

GO - Molecular functioni

  • protein complex binding Source: MGI
  • scaffold protein binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi201705. 8 interactions.
IntActiO55033. 4 interactions.
MINTiMINT-100584.
STRINGi10090.ENSMUSP00000083611.

Structurei

3D structure databases

ProteinModelPortaliO55033.
SMRiO55033. Positions 1-59, 114-170, 198-256, 284-380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 6160SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini111 – 17060SH3 2PROSITE-ProRule annotationAdd
BLAST
Domaini195 – 25763SH3 3PROSITE-ProRule annotationAdd
BLAST
Domaini285 – 37995SH2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 3 SH3 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG4226. Eukaryota.
ENOG410XRPF. LUCA.
GeneTreeiENSGT00820000126999.
HOGENOMiHOG000290684.
HOVERGENiHBG000719.
InParanoidiO55033.
KOiK19862.
OMAiQPALDCG.
OrthoDBiEOG7SV0VJ.
PhylomeDBiO55033.
TreeFamiTF351631.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR017304. NCK.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10663:SF159. PTHR10663:SF159. 1 hit.
PfamiPF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
PF14604. SH3_9. 1 hit.
[Graphical view]
PIRSFiPIRSF037874. Cytoplasmic_NCK. 1 hit.
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 3 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
PS50002. SH3. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55033-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEEVIVIAK WDYTAQQDQE LDIRKNERLW LLDDSKTWWR VRNAANRTGY
60 70 80 90 100
VPSNYVERKN SLKKGSLVKN LKDTLGLGKT RRKPSARDAS PTPSTDAEYP
110 120 130 140 150
ANGSGADRIY DLNIPAFVKF AYVAEREDEL SLVKGSRVTV MEKCSDGWWR
160 170 180 190 200
GSFNGQIGWF PSNYVLEEAD EAAAEAPSFL SLRRGTALSN GQGARVLHVV
210 220 230 240 250
QTLYPFSSVT EEELSFEKGE TMEVIEKPEN DPEWWKCKNA RGQVGLVPKN
260 270 280 290 300
YVVVLSDGPA LHPAHTPQIS YTGPSASGRF AGREWYYGNV TRHQAECALN
310 320 330 340 350
ERGVEGDFLI RDSESSPSDF SVSLKASGRN KHFKVQLVDS VYCIGQRRFH
360 370 380
SMDELVEHYK KAPIFTSEHG EKLYLVRALQ
Length:380
Mass (Da):42,879
Last modified:June 1, 1998 - v1
Checksum:i7B3404B976F0A54D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043260 mRNA. Translation: AAC06353.1.
AC107865 Genomic DNA. No translation available.
AC108914 Genomic DNA. No translation available.
CH466589 Genomic DNA. Translation: EDK96908.1.
BC011071 mRNA. Translation: AAH11071.1.
BC034255 mRNA. Translation: AAH34255.1.
CCDSiCCDS14924.1.
RefSeqiNP_035009.3. NM_010879.3.
XP_006495835.1. XM_006495772.2.
UniGeneiMm.389903.
Mm.483157.

Genome annotation databases

EnsembliENSMUST00000086421; ENSMUSP00000083611; ENSMUSG00000066877.
GeneIDi17974.
KEGGimmu:17974.
UCSCiuc007avo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043260 mRNA. Translation: AAC06353.1.
AC107865 Genomic DNA. No translation available.
AC108914 Genomic DNA. No translation available.
CH466589 Genomic DNA. Translation: EDK96908.1.
BC011071 mRNA. Translation: AAH11071.1.
BC034255 mRNA. Translation: AAH34255.1.
CCDSiCCDS14924.1.
RefSeqiNP_035009.3. NM_010879.3.
XP_006495835.1. XM_006495772.2.
UniGeneiMm.389903.
Mm.483157.

3D structure databases

ProteinModelPortaliO55033.
SMRiO55033. Positions 1-59, 114-170, 198-256, 284-380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201705. 8 interactions.
IntActiO55033. 4 interactions.
MINTiMINT-100584.
STRINGi10090.ENSMUSP00000083611.

PTM databases

iPTMnetiO55033.
PhosphoSiteiO55033.

Proteomic databases

EPDiO55033.
MaxQBiO55033.
PaxDbiO55033.
PRIDEiO55033.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000086421; ENSMUSP00000083611; ENSMUSG00000066877.
GeneIDi17974.
KEGGimmu:17974.
UCSCiuc007avo.2. mouse.

Organism-specific databases

CTDi8440.
MGIiMGI:1306821. Nck2.

Phylogenomic databases

eggNOGiKOG4226. Eukaryota.
ENOG410XRPF. LUCA.
GeneTreeiENSGT00820000126999.
HOGENOMiHOG000290684.
HOVERGENiHBG000719.
InParanoidiO55033.
KOiK19862.
OMAiQPALDCG.
OrthoDBiEOG7SV0VJ.
PhylomeDBiO55033.
TreeFamiTF351631.

Enzyme and pathway databases

ReactomeiR-MMU-186763. Downstream signal transduction.
R-MMU-373753. Nephrin interactions.
R-MMU-3928664. Ephrin signaling.
R-MMU-4420097. VEGFA-VEGFR2 Pathway.

Miscellaneous databases

NextBioi292929.
PROiO55033.
SOURCEiSearch...

Gene expression databases

BgeeiO55033.
ExpressionAtlasiO55033. baseline and differential.
GenevisibleiO55033. MM.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR017304. NCK.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10663:SF159. PTHR10663:SF159. 1 hit.
PfamiPF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
PF14604. SH3_9. 1 hit.
[Graphical view]
PIRSFiPIRSF037874. Cytoplasmic_NCK. 1 hit.
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 3 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
PS50002. SH3. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. She H.Y., Chen M., Li W.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  6. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
    Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
    J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Mast cell.
  7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90; SER-94 AND TYR-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiNCK2_MOUSE
AccessioniPrimary (citable) accession number: O55033
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: June 1, 1998
Last modified: May 11, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.