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O55029

- COPB2_MOUSE

UniProt

O55029 - COPB2_MOUSE

Protein

Coatomer subunit beta'

Gene

Copb2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (29 Aug 2001)
      Previous versions | rss
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    Functioni

    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors By similarity.By similarity
    This coatomer complex protein, essential for Golgi budding and vesicular trafficking, is a selective binding protein (RACK) for protein kinase C, epsilon type. It binds to Golgi membranes in a GTP-dependent manner.

    GO - Molecular functioni

    1. structural molecule activity Source: InterPro

    GO - Biological processi

    1. intracellular protein transport Source: InterPro
    2. intra-Golgi vesicle-mediated transport Source: Ensembl

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_227904. COPI Mediated Transport.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Coatomer subunit beta'
    Alternative name(s):
    Beta'-coat protein
    Short name:
    Beta'-COP
    p102
    Gene namesi
    Name:Copb2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1354962. Copb2.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication. Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cytoplasmic vesicleCOPI-coated vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. Shows only a slight preference for the cis-Golgi apparatus, compared with the trans-Golgi.By similarity

    GO - Cellular componenti

    1. actin cytoskeleton Source: Ensembl
    2. COPI vesicle coat Source: Ensembl
    3. cytosol Source: UniProtKB-SubCell
    4. Golgi apparatus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 905904Coatomer subunit beta'PRO_0000050913Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei627 – 6271N6-acetyllysineBy similarity
    Modified residuei859 – 8591PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO55029.
    PaxDbiO55029.
    PRIDEiO55029.

    PTM databases

    PhosphoSiteiO55029.

    Expressioni

    Gene expression databases

    BgeeiO55029.
    GenevestigatoriO55029.

    Interactioni

    Subunit structurei

    Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Probably interacts with PEX11A. Interacts with SCYL1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi206128. 3 interactions.
    IntActiO55029. 6 interactions.
    MINTiMINT-1666570.
    STRINGi10090.ENSMUSP00000035033.

    Structurei

    3D structure databases

    ProteinModelPortaliO55029.
    SMRiO55029. Positions 3-777.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati13 – 5240WD 1Add
    BLAST
    Repeati55 – 9440WD 2Add
    BLAST
    Repeati97 – 13640WD 3Add
    BLAST
    Repeati140 – 18041WD 4Add
    BLAST
    Repeati183 – 22442WD 5Add
    BLAST
    Repeati227 – 26640WD 6Add
    BLAST
    Repeati350 – 38839WD 7Add
    BLAST
    Repeati390 – 42536WD 8Add
    BLAST
    Repeati746 – 78338WD 9Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili867 – 89125Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat COPB2 family.Curated
    Contains 9 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00740000115578.
    HOGENOMiHOG000163444.
    HOVERGENiHBG051076.
    InParanoidiO55029.
    KOiK17302.
    OMAiIAHNPNG.
    OrthoDBiEOG78PV85.
    PhylomeDBiO55029.
    TreeFamiTF300688.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR006692. Coatomer_WD-assoc_reg.
    IPR016453. COPB2.
    IPR020472. G-protein_beta_WD-40_rep.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF04053. Coatomer_WDAD. 1 hit.
    PF00400. WD40. 5 hits.
    [Graphical view]
    PIRSFiPIRSF005567. Coatomer_beta'_subunit. 1 hit.
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 6 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 3 hits.
    PROSITEiPS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O55029-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLRLDIKRK LTARSDRVKS VDLHPTEPWM LASLYNGSVC VWNHETQTLV    50
    KTFEVCDLPV RAAKFVARKN WVVTGADDMQ IRVFNYNTLE RVHMFEAHSD 100
    YIRCIAVHPT QPFILTSSDD MLIKLWDWDK KWSCSQVFEG HTHYVMQIVI 150
    NPKDNNQFAS ASLDRTIKVW QLGSSSPNFT LEGHEKGVNC IDYYSGGDKP 200
    YLISGADDRL VKIWDYQNKT CVQTLEGHAQ NVSCASFHPE LPIIITGSED 250
    GTVRIWHSST YRLESTLNYG MERVWCVASL RGSNNVALGY DEGSIIVKLG 300
    REEPAMSMDA NGKIIWAKHS EVQQANLKAM GDTEIKDGER LPLAVKDMGS 350
    CEIYPQTIQH NPNGRFVVVC GDGEYIIYTA MALRNKSFGS AQEFAWAHDS 400
    SEYAIRESNS IVKIFKNFKE KKSFKPDFGA ESIYGGFLLG VRSVNGLAFY 450
    DWENTELIRR IEIQPKHIFW SDSGELVCIA TEESFFILKY LSEKVLAAQE 500
    THEGVTEDGI EDAFEVLGEI QEIVKTGLWV GDCFIYTSSV NRLNYYVGGE 550
    IVTIAHLDRT MYLLGYIPKD NRLYLGDKEL NIVSYSLLVS VLEYQTAVMR 600
    RDFSMADKVL PTIPKEQRTR VAHFLEKQGF KQQALTVSTD PEHRFELALQ 650
    LGELKIAYQL AVEAESEQKW KQLAELAISK CQFSLAQECL HHAQDYGGLL 700
    LLATASGNAS MVNKLAEGAE RDGKNNVAFM SYFLQGKLDA CLELLIRTGR 750
    LPEAAFLART YLPSQVSRVV KLWRENLSKV NQKAAESLAD PTEYENLFPG 800
    LKEAFVVEEW VKETHADLWP AKQYPLVTPN EERNVMEEAK GFQPSRPTAQ 850
    QEPDGKPASS PVIMASQTTH KEEKSLLELE VDLDNLELED IDTTDINLDE 900
    DILDD 905
    Length:905
    Mass (Da):102,449
    Last modified:August 29, 2001 - v2
    Checksum:i5845082CAB10FD12
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti684 – 6841S → H in AAB97760. 1 PublicationCurated
    Sequence conflicti733 – 7331F → L in AAB97760. 1 PublicationCurated
    Sequence conflicti839 – 8391A → P in AAB97760. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK088064 mRNA. Translation: BAC40125.1.
    AK153352 mRNA. Translation: BAE31926.1.
    AF043120 mRNA. Translation: AAB97760.1.
    BC006675 mRNA. Translation: AAH06675.1.
    CCDSiCCDS40734.1.
    RefSeqiNP_056642.1. NM_015827.2.
    UniGeneiMm.400464.

    Genome annotation databases

    EnsembliENSMUST00000035033; ENSMUSP00000035033; ENSMUSG00000032458.
    GeneIDi50797.
    KEGGimmu:50797.
    UCSCiuc009rdm.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK088064 mRNA. Translation: BAC40125.1 .
    AK153352 mRNA. Translation: BAE31926.1 .
    AF043120 mRNA. Translation: AAB97760.1 .
    BC006675 mRNA. Translation: AAH06675.1 .
    CCDSi CCDS40734.1.
    RefSeqi NP_056642.1. NM_015827.2.
    UniGenei Mm.400464.

    3D structure databases

    ProteinModelPortali O55029.
    SMRi O55029. Positions 3-777.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 206128. 3 interactions.
    IntActi O55029. 6 interactions.
    MINTi MINT-1666570.
    STRINGi 10090.ENSMUSP00000035033.

    PTM databases

    PhosphoSitei O55029.

    Proteomic databases

    MaxQBi O55029.
    PaxDbi O55029.
    PRIDEi O55029.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000035033 ; ENSMUSP00000035033 ; ENSMUSG00000032458 .
    GeneIDi 50797.
    KEGGi mmu:50797.
    UCSCi uc009rdm.1. mouse.

    Organism-specific databases

    CTDi 9276.
    MGIi MGI:1354962. Copb2.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00740000115578.
    HOGENOMi HOG000163444.
    HOVERGENi HBG051076.
    InParanoidi O55029.
    KOi K17302.
    OMAi IAHNPNG.
    OrthoDBi EOG78PV85.
    PhylomeDBi O55029.
    TreeFami TF300688.

    Enzyme and pathway databases

    Reactomei REACT_227904. COPI Mediated Transport.

    Miscellaneous databases

    NextBioi 307805.
    PROi O55029.
    SOURCEi Search...

    Gene expression databases

    Bgeei O55029.
    Genevestigatori O55029.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR006692. Coatomer_WD-assoc_reg.
    IPR016453. COPB2.
    IPR020472. G-protein_beta_WD-40_rep.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF04053. Coatomer_WDAD. 1 hit.
    PF00400. WD40. 5 hits.
    [Graphical view ]
    PIRSFi PIRSF005567. Coatomer_beta'_subunit. 1 hit.
    PRINTSi PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 6 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 3 hits.
    PROSITEi PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Bone marrow and Thymus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. Tarsounas M., Moens P.B., Pearlman R.E.
      Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 456-905.
      Tissue: Testis.
    4. Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCOPB2_MOUSE
    AccessioniPrimary (citable) accession number: O55029
    Secondary accession number(s): Q3U5Z9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: August 29, 2001
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3