Reviewed, UniProtKB/Swiss-Prot O55023 (IMPA1_MOUSE)

Names and origin

Protein names

Recommended name:
    Inositol monophosphatase
    EC=3.1.3.25
Alternative name(s):
    Inositol-1(or 4)-monophosphatase
      Short name=IMPase
      Short name=IMP
    Lithium-sensitive myo-inositol monophosphatase A1

Gene names

Name:

Impa1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Can use myo-inositol monophosphates, myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates By similarity.
Catalytic activity	Myo-inositol phosphate + H2O = myo-inositol + phosphate.
Cofactor	Magnesium.
Enzyme regulation	Inhibited by Li+.
Pathway	Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Mostly expressed in brain, small intestine, testis, kidney, and spleen (at protein level). Ref.3
Induction	By lithium Li+ in hippocamp. Ref.2
Sequence similarities	Belongs to the inositol monophosphatase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Lithium Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	inositol metabolic process Traceable author statement. Source: MGI
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inositol-1(or 4)-monophosphatase activity Inferred from electronic annotation. Source: EC lithium ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Inositol monophosphatase		PRO_0000142514
Regions
	Region	92 – 95	4	Substrate binding By similarity
	Region	194 – 196	3	Substrate binding By similarity
Sites
	Metal binding	70	1	Magnesium 1 By similarity
	Metal binding	90	1	Magnesium 1 By similarity
	Metal binding	90	1	Magnesium 2 By similarity
	Metal binding	92	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	93	1	Magnesium 2 By similarity
	Metal binding	220	1	Magnesium 2 By similarity
	Binding site	70	1	Substrate By similarity
	Binding site	213	1	Substrate By similarity
	Binding site	220	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O55023-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: EDBDB7BD24748E9D	FASTA	277	30,436
	10 20 30 40 50 60 MADPWQECMD YAVILARQAG EMIREALKNE MDVMIKSSPA DLVTVTDQKV EKMLMSSIKE 70 80 90 100 110 120 KYPCHSFIGE ESVAAGEKTV FTESPTWFID PIDGTTNFVH RFPFVAVSIG FLVNKEMEFG 130 140 150 160 170 180 IVYSCVEDKM YTGRKGKGAF CNGQKLQVSQ QEDITKSLLV TELGSSRKPE TLRIVLSNME 190 200 210 220 230 240 KLCSIPIHGI RSVGTAAVNM CLVATGGADA YYEMGIHCWD MAGAGIIVTE AGGVLMDVTG 250 260 270 GPFDLMSRRI IAANSITLAK RIAKEIEIIP LQRDDES

References

[1]	Parthasarathy L., Parthasarathy R., Vadnal R.E. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The effect of lithium on expression of genes for inositol biosynthetic enzymes in mouse hippocampus; a comparison with the yeast model." Shamir A., Shaltiel G., Greenberg M.L., Belmaker R.H., Agam G. Brain Res. Mol. Brain Res. 115:104-110(2003) [PubMed: 12877981] [Abstract] Cited for: INDUCTION.
[3]	"Spatial expression patterns and biochemical properties distinguish a second myo-inositol monophosphatase IMPA2 from IMPA1." Ohnishi T., Ohba H., Seo K.-C., Im J., Sato Y., Iwayama Y., Furuichi T., Chung S.-K., Yoshikawa T. J. Biol. Chem. 282:637-646(2007) [PubMed: 17068342] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF042730 mRNA. Translation: AAB97469.1.
IPI	IPI00115085.
UniGene	Mm.183042
3D structure databases
HSSP	HSSP built from PDB template 1IMF based on UniProtKB P29218.
SMR	O55023. Positions 3-276.
ModBase	Search...
Protein-protein interaction databases
STRING	O55023.
PTM databases
PhosphoSite	O55023.
Proteomic databases
PRIDE	O55023.
Genome annotation databases
Ensembl	ENSMUST00000065938; ENSMUSP00000068174; ENSMUSG00000027531; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:1933158. Impa1.
Phylogenomic databases
HOGENOM	O55023.
HOVERGEN	O55023.
Enzyme and pathway databases
BRENDA	3.1.3.25. 244.
Gene expression databases
ArrayExpress	O55023.
Bgee	O55023.
CleanEx	MM_IMPA1.
Genevestigator	O55023.
GermOnline	ENSMUSG00000027531. Mus musculus.
Family and domain databases
InterPro	IPR020583. Inositol_monoP_metal-BS. IPR020552. Inositol_monoPase_Li-sen. IPR000760. Inositol_monophosphatase. IPR020550. Inositol_monophosphatase_CS. [Graphical view]
PANTHER	PTHR20854. Inositol_P. 1 hit.
Pfam	PF00459. Inositol_P. 1 hit. [Graphical view]
PRINTS	PR00377. IMPHPHTASES. PR00378. LIIMPHPHTASE.
ProDom	PD023420. Inositol_P. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00629. IMP_1. 1 hit. PS00630. IMP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

IMPA1_MOUSE

Accession

Primary (citable) accession number: O55023

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	October 13, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)