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O55023

- IMPA1_MOUSE

UniProt

O55023 - IMPA1_MOUSE

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Protein

Inositol monophosphatase 1

Gene

Impa1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Has broad substrate specificity and can use myo-inositol monophosphates, myo-inositol 1,3-diphosphate, myo-inositol 1,4-diphosphate, scyllo-inositol-phosphate, D-galactose 1-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates.By similarity

Catalytic activityi

Myo-inositol phosphate + H2O = myo-inositol + phosphate.By similarity
Alpha-D-galactose 1-phosphate + H2O = D-galactose + phosphate.By similarity

Cofactori

Magnesium.1 Publication

Enzyme regulationi

Inhibited by Li+, Ca2+ and Mn2+, but also by Mg2+ at concentrations above 3 mM.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Magnesium 1; catalytic1 Publication
Binding sitei70 – 701SubstrateCurated
Metal bindingi90 – 901Magnesium 1; catalytic1 Publication
Metal bindingi90 – 901Magnesium 21 Publication
Metal bindingi92 – 921Magnesium 1; via carbonyl oxygen; catalytic1 Publication
Metal bindingi93 – 931Magnesium 21 Publication
Binding sitei213 – 2131SubstrateBy similarity
Metal bindingi220 – 2201Magnesium 21 Publication
Binding sitei220 – 2201SubstrateBy similarity

GO - Molecular functioni

  1. inositol monophosphate 1-phosphatase activity Source: MGI
  2. inositol monophosphate 3-phosphatase activity Source: UniProtKB-EC
  3. inositol monophosphate 4-phosphatase activity Source: UniProtKB-EC
  4. inositol monophosphate phosphatase activity Source: UniProtKB
  5. lithium ion binding Source: UniProtKB
  6. magnesium ion binding Source: UniProtKB
  7. manganese ion binding Source: UniProtKB
  8. protein homodimerization activity Source: MGI

GO - Biological processi

  1. inositol biosynthetic process Source: UniProtKB-UniPathway
  2. inositol metabolic process Source: MGI
  3. inositol phosphate dephosphorylation Source: UniProtKB
  4. phosphatidylinositol phosphorylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Lithium, Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00823; UER00788.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol monophosphatase 1 (EC:3.1.3.25By similarity)
Short name:
IMP 1
Short name:
IMPase 1
Alternative name(s):
D-galactose 1-phosphate phosphataseBy similarity (EC:3.1.3.94By similarity)
Inositol-1(or 4)-monophosphatase 1
Lithium-sensitive myo-inositol monophosphatase A1
Gene namesi
Name:Impa1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1933158. Impa1.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 277277Inositol monophosphatase 1PRO_0000142514Add
BLAST

Proteomic databases

MaxQBiO55023.
PaxDbiO55023.
PRIDEiO55023.

PTM databases

PhosphoSiteiO55023.

Expressioni

Tissue specificityi

Mostly expressed in brain, small intestine, testis, kidney, and spleen (at protein level).1 Publication

Inductioni

By lithium Li+ in hippocamp.1 Publication

Gene expression databases

CleanExiMM_IMPA1.
GenevestigatoriO55023.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiO55023. 1 interaction.

Structurei

Secondary structure

1
277
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 2623
Beta strandi34 – 385
Helixi45 – 6117
Beta strandi65 – 695
Helixi70 – 745
Beta strandi86 – 938
Helixi95 – 1006
Beta strandi106 – 1138
Beta strandi116 – 1249
Turni125 – 1284
Beta strandi129 – 1346
Turni135 – 1373
Beta strandi138 – 1414
Helixi154 – 1563
Beta strandi158 – 1603
Helixi169 – 18315
Turni184 – 1863
Beta strandi188 – 1925
Helixi196 – 2049
Beta strandi207 – 2159
Helixi218 – 23013
Beta strandi234 – 2363
Beta strandi240 – 2423
Beta strandi247 – 2548
Helixi256 – 26510

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AS5X-ray2.43A/B/C/D1-277[»]
ProteinModelPortaliO55023.
SMRiO55023. Positions 3-276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni90 – 956Substrate bindingCurated
Regioni194 – 1963Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the inositol monophosphatase family.Curated

Phylogenomic databases

eggNOGiCOG0483.
HOGENOMiHOG000282238.
HOVERGENiHBG052123.
InParanoidiO55023.
PhylomeDBiO55023.

Family and domain databases

InterProiIPR020583. Inositol_monoP_metal-BS.
IPR020552. Inositol_monoPase_Li-sen.
IPR000760. Inositol_monophosphatase.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 1 hit.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
PRINTSiPR00377. IMPHPHTASES.
PR00378. LIIMPHPHTASE.
PROSITEiPS00629. IMP_1. 1 hit.
PS00630. IMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O55023-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADPWQECMD YAVILARQAG EMIREALKNE MDVMIKSSPA DLVTVTDQKV
60 70 80 90 100
EKMLMSSIKE KYPCHSFIGE ESVAAGEKTV FTESPTWFID PIDGTTNFVH
110 120 130 140 150
RFPFVAVSIG FLVNKEMEFG IVYSCVEDKM YTGRKGKGAF CNGQKLQVSQ
160 170 180 190 200
QEDITKSLLV TELGSSRKPE TLRIVLSNME KLCSIPIHGI RSVGTAAVNM
210 220 230 240 250
CLVATGGADA YYEMGIHCWD MAGAGIIVTE AGGVLMDVTG GPFDLMSRRI
260 270
IAANSITLAK RIAKEIEIIP LQRDDES
Length:277
Mass (Da):30,436
Last modified:June 1, 1998 - v1
Checksum:iEDBDB7BD24748E9D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF042730 mRNA. Translation: AAB97469.1.
CCDSiCCDS17240.1.
UniGeneiMm.183042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF042730 mRNA. Translation: AAB97469.1 .
CCDSi CCDS17240.1.
UniGenei Mm.183042.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4AS5 X-ray 2.43 A/B/C/D 1-277 [» ]
ProteinModelPortali O55023.
SMRi O55023. Positions 3-276.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O55023. 1 interaction.

PTM databases

PhosphoSitei O55023.

Proteomic databases

MaxQBi O55023.
PaxDbi O55023.
PRIDEi O55023.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:1933158. Impa1.

Phylogenomic databases

eggNOGi COG0483.
HOGENOMi HOG000282238.
HOVERGENi HBG052123.
InParanoidi O55023.
PhylomeDBi O55023.

Enzyme and pathway databases

UniPathwayi UPA00823 ; UER00788 .

Miscellaneous databases

ChiTaRSi IMPA1. mouse.
PROi O55023.
SOURCEi Search...

Gene expression databases

CleanExi MM_IMPA1.
Genevestigatori O55023.

Family and domain databases

InterProi IPR020583. Inositol_monoP_metal-BS.
IPR020552. Inositol_monoPase_Li-sen.
IPR000760. Inositol_monophosphatase.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view ]
PANTHERi PTHR20854. PTHR20854. 1 hit.
Pfami PF00459. Inositol_P. 1 hit.
[Graphical view ]
PRINTSi PR00377. IMPHPHTASES.
PR00378. LIIMPHPHTASE.
PROSITEi PS00629. IMP_1. 1 hit.
PS00630. IMP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Parthasarathy L., Parthasarathy R., Vadnal R.E.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The effect of lithium on expression of genes for inositol biosynthetic enzymes in mouse hippocampus; a comparison with the yeast model."
    Shamir A., Shaltiel G., Greenberg M.L., Belmaker R.H., Agam G.
    Brain Res. Mol. Brain Res. 115:104-110(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  3. "Spatial expression patterns and biochemical properties distinguish a second myo-inositol monophosphatase IMPA2 from IMPA1."
    Ohnishi T., Ohba H., Seo K.-C., Im J., Sato Y., Iwayama Y., Furuichi T., Chung S.-K., Yoshikawa T.
    J. Biol. Chem. 282:637-646(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "Cloning, expression, purification, crystallization and X-ray analysis of inositol monophosphatase from Mus musculus and Homo sapiens."
    Singh N., Halliday A.C., Knight M., Lack N.A., Lowe E., Churchill G.C.
    Acta Crystallogr. F 68:1149-1152(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.43 ANGSTROMS) IN COMPLEX WITH MAGNESIUM AND PHOSPHATE, SUBUNIT, COFACTOR.

Entry informationi

Entry nameiIMPA1_MOUSE
AccessioniPrimary (citable) accession number: O55023
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3