O55022 (PGRC1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Membrane-associated progesterone receptor component 1

Gene names

Name:	Pgrmc1
Synonyms:	Pgrmc

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for progesterone By similarity.
Subcellular location	Microsome membrane; Single-pass membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.
Domain	The cytochrome b5 heme-binding domain lacks the conserved iron-binding His residues at positions 107 and 131 By similarity.
Sequence similarities	Belongs to the cytochrome b5 family. MAPR subfamily. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Ligand	Lipid-binding Steroid-binding
Molecular function	Receptor
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	axon guidance Inferred from electronic annotation. Source: Compara
Cellular_component	cell surface Inferred from electronic annotation. Source: Compara endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleolus Inferred from electronic annotation. Source: Compara
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro steroid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 195	194	Membrane-associated progesterone receptor component 1		PRO_0000121740
Regions
	Topological domain	2 – 24	23	Lumenal Potential
	Transmembrane	25 – 43	19	Helical; Potential
	Topological domain	44 – 195	152	Cytoplasmic Potential
	Domain	72 – 171	100	Cytochrome b5 heme-binding
Amino acid modifications
	Modified residue	54	1	Phosphoserine By similarity
	Modified residue	178	1	Phosphothreonine By similarity
	Modified residue	180	1	Phosphotyrosine Ref.6 Ref.8
	Modified residue	181	1	Phosphoserine Ref.4 Ref.5 Ref.7 Ref.8 Ref.9 Ref.10
Experimental info
	Sequence conflict	87	1	P → S in AAB97466. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O55022 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 1FFD17AFAE6C81FC	FASTA	195	21,694
	10 20 30 40 50 60 MAAEDVVATG ADPSELEGGG LLHEIFTSPL NLLLLGLCIF LLYKIVRGDQ PGASGDNDDD 70 80 90 100 110 120 EPPPLPRLKR RDFTPAELRR FDGVQDPRIL MAINGKVFDV TKGRKFYGPE GPYGVFAGRD 130 140 150 160 170 180 ASRGLATFCL DKEALKDEYD DLSDLTPAQQ ETLSDWDSQF TFKYHHVGKL LKEGEEPTVY 190 SDDEEPKDET ARKNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The expression of a putative membrane associated progesterone receptor component in the mouse testis and epididymis." Kwon S., Lunn R.M., O'Brien D.A., Bell D.A., Eddy E.M. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 72-88 AND 106-119, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[4]	"Phosphoproteome analysis of mouse liver using immobilized metal affinity purification and linear ion trap mass spectrometry." Jin W.-H., Dai J., Zhou H., Xia Q.-C., Zou H.-F., Zeng R. Rapid Commun. Mass Spectrom. 18:2169-2176(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-181.
[5]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-181, MASS SPECTROMETRY. Tissue: Liver.
[6]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-180, MASS SPECTROMETRY. Tissue: Brain cortex.
[7]	"Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS." Lee J., Xu Y., Chen Y., Sprung R., Kim S.C., Xie S., Zhao Y. Mol. Cell. Proteomics 6:669-676(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-181, MASS SPECTROMETRY. Tissue: Liver.
[8]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-180 AND SER-181, MASS SPECTROMETRY. Tissue: Liver.
[9]	"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis." Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H. J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-181, MASS SPECTROMETRY. Tissue: Liver.
[10]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-181, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF042491 mRNA. Translation: AAB97466.1. BC006016 mRNA. Translation: AAH06016.1.
IPI	IPI00319973.
RefSeq	NP_058063.2. NM_016783.3.
UniGene	Mm.9052.
3D structure databases
ProteinModelPortal	O55022.
SMR	O55022. Positions 72-171.
ModBase	Search...
Protein-protein interaction databases
IntAct	O55022. 8 interactions.
PTM databases
PhosphoSite	O55022.
Proteomic databases
PaxDb	O55022.
PRIDE	O55022.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000073339; ENSMUSP00000073061; ENSMUSG00000006373.
GeneID	53328.
KEGG	mmu:53328.
Organism-specific databases
CTD	10857.
MGI	MGI:1858305. Pgrmc1.
Phylogenomic databases
eggNOG	NOG291734.
HOGENOM	HOG000187840.
HOVERGEN	HBG059971.
InParanoid	O55022.
OrthoDB	EOG4SXNDQ.
Gene expression databases
ArrayExpress	O55022.
Bgee	O55022.
CleanEx	MM_PGRMC1.
Genevestigator	O55022.
GermOnline	ENSMUSG00000006373. Mus musculus.
Family and domain databases
Gene3D	3.10.120.10. 1 hit.
InterPro	IPR001199. Cyt_B5-like_heme/steroid-bd. [Graphical view]
Pfam	PF00173. Cyt-b5. 1 hit. [Graphical view]
SUPFAM	SSF55856. Cyt_B5. 1 hit.
PROSITE	PS00191. CYTOCHROME_B5_1. False negative. PS50255. CYTOCHROME_B5_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	310145.
SOURCE	Search...

Entry information

Entry name

PGRC1_MOUSE

Accession

Primary (citable) accession number: O55022
Secondary accession number(s): Q99JN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 107 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program