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Protein

Phosphatidylinositol-binding clathrin assembly protein

Gene

Picalm

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.By similarity

GO - Molecular functioni

  • 1-phosphatidylinositol binding Source: UniProtKB
  • clathrin adaptor activity Source: Alzheimers_University_of_Toronto
  • clathrin binding Source: UniProtKB
  • identical protein binding Source: IntAct
  • SH3 domain binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol-binding clathrin assembly protein
Alternative name(s):
Clathrin assembly lymphoid myeloid leukemia protein
Short name:
rCALM
Gene namesi
Name:Picalm
Synonyms:Calm
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621054. Picalm.

Subcellular locationi

  • Membraneclathrin-coated pit By similarity
  • Golgi apparatus By similarity
  • Cytoplasmic vesicleclathrin-coated vesicle By similarity
  • Nucleus By similarity

  • Note: Colocalized with clathrin in the Golgi area. Interaction with FAM64A may target PICALM to the nucleus in some cells.By similarity

GO - Cellular componenti

  • clathrin-coated pit Source: UniProtKB
  • clathrin-coated vesicle Source: BHF-UCL
  • endosome Source: BHF-UCL
  • Golgi apparatus Source: UniProtKB-SubCell
  • neurofibrillary tangle Source: Alzheimers_University_of_Toronto
  • neuronal cell body Source: Alzheimers_University_of_Toronto
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: BHF-UCL
  • postsynaptic density Source: BHF-UCL
  • postsynaptic membrane Source: BHF-UCL
  • presynaptic membrane Source: BHF-UCL
  • synapse Source: RGD
  • synaptic vesicle Source: BHF-UCL
  • vesicle Source: Alzheimers_University_of_Toronto
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001870642 – 640Phosphatidylinositol-binding clathrin assembly proteinAdd BLAST639

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei16PhosphoserineBy similarity1
Modified residuei20PhosphoserineBy similarity1
Cross-linki238Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei303PhosphoserineBy similarity1
Modified residuei315PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PeptideAtlasiO55012.
PRIDEiO55012.

PTM databases

iPTMnetiO55012.
PhosphoSitePlusiO55012.

Expressioni

Tissue specificityi

Isoform 2 was found in most tissues examined. Isoform 1 has an overlapping expression pattern but is absent from lung, heart and pancreas. Both isoforms are widely expressed in the brain, higher levels are seen in hippocampus, dentate gyrus, medial habenula nucleus and cerebellar granule cells.1 Publication

Interactioni

Subunit structurei

Binds clathrin; involves primarily the C-terminal sequences, but the full-length protein is required for full binding capacity. Binds phosphatidylinositol 4,5- bisphosphate. Interacts with FAM64A; this interaction may change the subcellular location into the nucleus.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-915601,EBI-915601
VAMP2P630272EBI-915601,EBI-520113From a different organism.
VAMP3Q158362EBI-915601,EBI-722343From a different organism.
Vamp8O704044EBI-915601,EBI-1812572From a different organism.

GO - Molecular functioni

  • clathrin adaptor activity Source: Alzheimers_University_of_Toronto
  • clathrin binding Source: UniProtKB
  • identical protein binding Source: IntAct
  • SH3 domain binding Source: RGD

Protein-protein interaction databases

BioGridi250139. 1 interactor.
IntActiO55012. 4 interactors.
MINTiMINT-4575979.

Structurei

Secondary structure

1640
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 29Combined sources24
Beta strandi32 – 35Combined sources4
Helixi39 – 50Combined sources12
Helixi56 – 66Combined sources11
Helixi72 – 88Combined sources17
Helixi91 – 99Combined sources9
Helixi115 – 141Combined sources27
Turni145 – 147Combined sources3
Beta strandi151 – 154Combined sources4
Helixi155 – 158Combined sources4
Helixi161 – 179Combined sources19
Helixi185 – 187Combined sources3
Helixi191 – 220Combined sources30
Helixi222 – 224Combined sources3
Helixi227 – 256Combined sources30
Helixi261 – 263Combined sources3
Helixi267 – 269Combined sources3
Helixi275 – 285Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HF8X-ray2.00A1-289[»]
1HFAX-ray2.00A1-289[»]
1HG2X-ray2.00A1-289[»]
1HG5X-ray2.00A1-289[»]
3ZYKX-ray1.80A/B1-289[»]
3ZYLX-ray1.70A/B1-264[»]
3ZYMX-ray2.03A/B/C1-264[»]
ProteinModelPortaliO55012.
SMRiO55012.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO55012.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 145ENTHPROSITE-ProRule annotationCuratedAdd BLAST132

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni221 – 294Interaction with FAM64ABy similarityAdd BLAST74

Sequence similaritiesi

Belongs to the PICALM/SNAP91 family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG049391.
InParanoidiO55012.
KOiK20044.
PhylomeDBiO55012.

Family and domain databases

Gene3Di1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR011417. ANTH_dom.
IPR014712. Clathrin_AP_2.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR030412. PICALM.
[Graphical view]
PANTHERiPTHR22951:SF16. PTHR22951:SF16. 2 hits.
PfamiPF07651. ANTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: O55012-1) [UniParc]FASTAAdd to basket
Also known as: Long1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGQSLTDRI TAAQHSVTGS AVSKTVCKAT THEIMGPKKK HLDYLIQCTN
60 70 80 90 100
EMNVNIPQLA DSLFERTTNS SWVVVFKSLI TTHHLMVYGN ERFIQYLASR
110 120 130 140 150
NTLFNLSNFL DKSGLQGYDM STFIRRYSRY LNEKAVSYRQ VAFDFTKVKR
160 170 180 190 200
GADGVMRTMN TEKLLKTVPI IQNQMDALLD FNVNSNELTN GVINAAFMLL
210 220 230 240 250
FKDAIRLFAA YNEGIINLLE KYFDMKKNQC KEGLDIYKKF LTRMTRISEF
260 270 280 290 300
LKVAEQVGID RGDIPDLSQA PSSLLDALEQ HLASLEGKKI KDSTAASRAT
310 320 330 340 350
TLSNAVSSLA STGLSLTKVD EREKQAALEE EQARLKALKE QRLKELAKKP
360 370 380 390 400
HTSLTTAASP VSTSAGGIMT APAIDIFSTP SSSNSTSKLP NDLLDLQQPT
410 420 430 440 450
FHPSVHAMSA APQVASTWGD AVDDAIPSLN PFLTKSSGDV HLPISSDVST
460 470 480 490 500
FTTRTPTHEM FVGFSPSPVT QPHPSAGLNV DFESVFGNKS TNVAVDSGGG
510 520 530 540 550
LLKPTVASQN QSLPVAKLPP NKLVSDDLDS SLANLVGNLG IGNGTTKNDV
560 570 580 590 600
SCSQPGEKKL TGGSNWQPKV APTTAWSAAT MAPPVMAYPA TTPTGMIGYG
610 620 630 640
IPPQMGSVPV MTQPTLIYSQ PVMRPPNPFG PVPGAQIQFM
Length:640
Mass (Da):69,286
Last modified:June 1, 1998 - v1
Checksum:i7395A92C285FA10A
GO
Isoform 21 Publication (identifier: O55012-2) [UniParc]FASTAAdd to basket
Also known as: Short1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     420-462: Missing.

Show »
Length:597
Mass (Da):64,657
Checksum:i42CA941426B9E6A4
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050687420 – 462Missing in isoform 2. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041373 mRNA. Translation: AAB97078.1.
AF041374 mRNA. Translation: AAB97079.1.
RefSeqiNP_446006.1. NM_053554.2. [O55012-1]
UniGeneiRn.24871.

Genome annotation databases

GeneIDi89816.
KEGGirno:89816.
UCSCiRGD:621054. rat. [O55012-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041373 mRNA. Translation: AAB97078.1.
AF041374 mRNA. Translation: AAB97079.1.
RefSeqiNP_446006.1. NM_053554.2. [O55012-1]
UniGeneiRn.24871.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HF8X-ray2.00A1-289[»]
1HFAX-ray2.00A1-289[»]
1HG2X-ray2.00A1-289[»]
1HG5X-ray2.00A1-289[»]
3ZYKX-ray1.80A/B1-289[»]
3ZYLX-ray1.70A/B1-264[»]
3ZYMX-ray2.03A/B/C1-264[»]
ProteinModelPortaliO55012.
SMRiO55012.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250139. 1 interactor.
IntActiO55012. 4 interactors.
MINTiMINT-4575979.

PTM databases

iPTMnetiO55012.
PhosphoSitePlusiO55012.

Proteomic databases

PeptideAtlasiO55012.
PRIDEiO55012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi89816.
KEGGirno:89816.
UCSCiRGD:621054. rat. [O55012-1]

Organism-specific databases

CTDi8301.
RGDi621054. Picalm.

Phylogenomic databases

HOVERGENiHBG049391.
InParanoidiO55012.
KOiK20044.
PhylomeDBiO55012.

Miscellaneous databases

EvolutionaryTraceiO55012.
PROiO55012.

Family and domain databases

Gene3Di1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR011417. ANTH_dom.
IPR014712. Clathrin_AP_2.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR030412. PICALM.
[Graphical view]
PANTHERiPTHR22951:SF16. PTHR22951:SF16. 2 hits.
PfamiPF07651. ANTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPICAL_RAT
AccessioniPrimary (citable) accession number: O55012
Secondary accession number(s): O55011
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.