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Protein

Phosphatidylinositol-binding clathrin assembly protein

Gene

Picalm

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.By similarity

GO - Molecular functioni

  • 1-phosphatidylinositol binding Source: UniProtKB
  • clathrin adaptor activity Source: Alzheimers_University_of_Toronto
  • clathrin binding Source: UniProtKB
  • identical protein binding Source: IntAct
  • SH3 domain binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol-binding clathrin assembly protein
Alternative name(s):
Clathrin assembly lymphoid myeloid leukemia protein
Short name:
rCALM
Gene namesi
Name:Picalm
Synonyms:Calm
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621054. Picalm.

Subcellular locationi

  • Membraneclathrin-coated pit By similarity
  • Golgi apparatus By similarity
  • Cytoplasmic vesicleclathrin-coated vesicle By similarity
  • Nucleus By similarity

  • Note: Colocalized with clathrin in the Golgi area. Interaction with FAM64A may target PICALM to the nucleus in some cells.By similarity

GO - Cellular componenti

  • clathrin-coated vesicle Source: BHF-UCL
  • coated pit Source: UniProtKB
  • endosome Source: BHF-UCL
  • Golgi apparatus Source: UniProtKB-SubCell
  • neurofibrillary tangle Source: Alzheimers_University_of_Toronto
  • neuronal cell body Source: Alzheimers_University_of_Toronto
  • neuronal postsynaptic density Source: BHF-UCL
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: BHF-UCL
  • postsynaptic membrane Source: BHF-UCL
  • presynaptic membrane Source: BHF-UCL
  • synapse Source: RGD
  • synaptic vesicle Source: BHF-UCL
  • vesicle Source: Alzheimers_University_of_Toronto
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 640639Phosphatidylinositol-binding clathrin assembly proteinPRO_0000187064Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei16 – 161PhosphoserineBy similarity
Cross-linki238 – 238Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiO55012.

PTM databases

iPTMnetiO55012.
PhosphoSiteiO55012.

Expressioni

Tissue specificityi

Isoform 2 was found in most tissues examined. Isoform 1 has an overlapping expression pattern but is absent from lung, heart and pancreas. Both isoforms are widely expressed in the brain, higher levels are seen in hippocampus, dentate gyrus, medial habenula nucleus and cerebellar granule cells.1 Publication

Interactioni

Subunit structurei

Binds clathrin; involves primarily the C-terminal sequences, but the full-length protein is required for full binding capacity. Binds phosphatidylinositol 4,5- bisphosphate. Interacts with FAM64A; this interaction may change the subcellular location into the nucleus.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-915601,EBI-915601
VAMP2P630272EBI-915601,EBI-520113From a different organism.
VAMP3Q158362EBI-915601,EBI-722343From a different organism.
Vamp8O704044EBI-915601,EBI-1812572From a different organism.

GO - Molecular functioni

  • clathrin adaptor activity Source: Alzheimers_University_of_Toronto
  • clathrin binding Source: UniProtKB
  • identical protein binding Source: IntAct
  • SH3 domain binding Source: RGD

Protein-protein interaction databases

BioGridi250139. 1 interaction.
IntActiO55012. 4 interactions.
MINTiMINT-4575979.

Structurei

Secondary structure

1
640
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 2924Combined sources
Beta strandi32 – 354Combined sources
Helixi39 – 5012Combined sources
Helixi56 – 6611Combined sources
Helixi72 – 8817Combined sources
Helixi91 – 999Combined sources
Helixi115 – 14127Combined sources
Turni145 – 1473Combined sources
Beta strandi151 – 1544Combined sources
Helixi155 – 1584Combined sources
Helixi161 – 17919Combined sources
Helixi185 – 1873Combined sources
Helixi191 – 22030Combined sources
Helixi222 – 2243Combined sources
Helixi227 – 25630Combined sources
Helixi261 – 2633Combined sources
Helixi267 – 2693Combined sources
Helixi275 – 28511Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HF8X-ray2.00A1-289[»]
1HFAX-ray2.00A1-289[»]
1HG2X-ray2.00A1-289[»]
1HG5X-ray2.00A1-289[»]
3ZYKX-ray1.80A/B1-289[»]
3ZYLX-ray1.70A/B1-264[»]
3ZYMX-ray2.03A/B/C1-264[»]
ProteinModelPortaliO55012.
SMRiO55012. Positions 19-281.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO55012.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 145132ENTHPROSITE-ProRule annotationCuratedAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni221 – 29474Interaction with FAM64ABy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the PICALM/SNAP91 family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG049391.
InParanoidiO55012.
PhylomeDBiO55012.

Family and domain databases

Gene3Di1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR011417. ANTH_dom.
IPR014712. Clathrin_AP_2.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR030412. PICALM.
[Graphical view]
PANTHERiPTHR22951:SF16. PTHR22951:SF16. 2 hits.
PfamiPF07651. ANTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: O55012-1) [UniParc]FASTAAdd to basket

Also known as: Long1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGQSLTDRI TAAQHSVTGS AVSKTVCKAT THEIMGPKKK HLDYLIQCTN
60 70 80 90 100
EMNVNIPQLA DSLFERTTNS SWVVVFKSLI TTHHLMVYGN ERFIQYLASR
110 120 130 140 150
NTLFNLSNFL DKSGLQGYDM STFIRRYSRY LNEKAVSYRQ VAFDFTKVKR
160 170 180 190 200
GADGVMRTMN TEKLLKTVPI IQNQMDALLD FNVNSNELTN GVINAAFMLL
210 220 230 240 250
FKDAIRLFAA YNEGIINLLE KYFDMKKNQC KEGLDIYKKF LTRMTRISEF
260 270 280 290 300
LKVAEQVGID RGDIPDLSQA PSSLLDALEQ HLASLEGKKI KDSTAASRAT
310 320 330 340 350
TLSNAVSSLA STGLSLTKVD EREKQAALEE EQARLKALKE QRLKELAKKP
360 370 380 390 400
HTSLTTAASP VSTSAGGIMT APAIDIFSTP SSSNSTSKLP NDLLDLQQPT
410 420 430 440 450
FHPSVHAMSA APQVASTWGD AVDDAIPSLN PFLTKSSGDV HLPISSDVST
460 470 480 490 500
FTTRTPTHEM FVGFSPSPVT QPHPSAGLNV DFESVFGNKS TNVAVDSGGG
510 520 530 540 550
LLKPTVASQN QSLPVAKLPP NKLVSDDLDS SLANLVGNLG IGNGTTKNDV
560 570 580 590 600
SCSQPGEKKL TGGSNWQPKV APTTAWSAAT MAPPVMAYPA TTPTGMIGYG
610 620 630 640
IPPQMGSVPV MTQPTLIYSQ PVMRPPNPFG PVPGAQIQFM
Length:640
Mass (Da):69,286
Last modified:June 1, 1998 - v1
Checksum:i7395A92C285FA10A
GO
Isoform 21 Publication (identifier: O55012-2) [UniParc]FASTAAdd to basket

Also known as: Short1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     420-462: Missing.

Show »
Length:597
Mass (Da):64,657
Checksum:i42CA941426B9E6A4
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei420 – 46243Missing in isoform 2. 1 PublicationVSP_050687Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041373 mRNA. Translation: AAB97078.1.
AF041374 mRNA. Translation: AAB97079.1.
RefSeqiNP_446006.1. NM_053554.2. [O55012-1]
UniGeneiRn.24871.

Genome annotation databases

GeneIDi89816.
KEGGirno:89816.
UCSCiRGD:621054. rat. [O55012-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041373 mRNA. Translation: AAB97078.1.
AF041374 mRNA. Translation: AAB97079.1.
RefSeqiNP_446006.1. NM_053554.2. [O55012-1]
UniGeneiRn.24871.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HF8X-ray2.00A1-289[»]
1HFAX-ray2.00A1-289[»]
1HG2X-ray2.00A1-289[»]
1HG5X-ray2.00A1-289[»]
3ZYKX-ray1.80A/B1-289[»]
3ZYLX-ray1.70A/B1-264[»]
3ZYMX-ray2.03A/B/C1-264[»]
ProteinModelPortaliO55012.
SMRiO55012. Positions 19-281.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250139. 1 interaction.
IntActiO55012. 4 interactions.
MINTiMINT-4575979.

PTM databases

iPTMnetiO55012.
PhosphoSiteiO55012.

Proteomic databases

PRIDEiO55012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi89816.
KEGGirno:89816.
UCSCiRGD:621054. rat. [O55012-1]

Organism-specific databases

CTDi8301.
RGDi621054. Picalm.

Phylogenomic databases

HOVERGENiHBG049391.
InParanoidiO55012.
PhylomeDBiO55012.

Miscellaneous databases

EvolutionaryTraceiO55012.
NextBioi617702.
PROiO55012.

Family and domain databases

Gene3Di1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR011417. ANTH_dom.
IPR014712. Clathrin_AP_2.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR030412. PICALM.
[Graphical view]
PANTHERiPTHR22951:SF16. PTHR22951:SF16. 2 hits.
PfamiPF07651. ANTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of clathrin assembly protein gene (rCALM) and its differential expression to AP180 in rat brain."
    Kim H.-L., Lee S.C.
    Exp. Mol. Med. 31:191-196(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Strain: Sprague-DawleyImported.
    Tissue: BrainImported.
  2. "Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes."
    Ford M.G., Pearse B.M., Higgins M.K., Vallis Y., Owen D.J., Gibson A., Hopkins C.R., Evans P.R., McMahon H.T.
    Science 291:1051-1055(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 19-281.

Entry informationi

Entry nameiPICAL_RAT
AccessioniPrimary (citable) accession number: O55012
Secondary accession number(s): O55011
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: June 1, 1998
Last modified: January 20, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.