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Protein

Ribonuclease 4

Gene

Rnase4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This RNase has marked specificity towards the 3' side of uridine nucleotides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381SubstrateBy similarity
Active sitei40 – 401Proton acceptorBy similarity
Binding sitei93 – 931SubstrateBy similarity
Binding sitei110 – 1101SubstrateBy similarity
Active sitei144 – 1441Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 4 (EC:3.1.27.-)
Short name:
RNase 4
Alternative name(s):
RL3
Gene namesi
Name:Rnase4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi61823. Rnase4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828By similarityAdd
BLAST
Chaini29 – 147119Ribonuclease 4PRO_0000030887Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291Pyrrolidone carboxylic acidBy similarity
Disulfide bondi53 ↔ 109By similarity
Disulfide bondi67 ↔ 120By similarity
Disulfide bondi85 ↔ 135By similarity
Disulfide bondi92 ↔ 99By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiO55004.
PRIDEiO55004.

Expressioni

Gene expression databases

ExpressionAtlasiO55004. baseline and differential.
GenevisibleiO55004. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043003.

Structurei

3D structure databases

ProteinModelPortaliO55004.
SMRiO55004. Positions 29-146.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni68 – 725Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG42321.
GeneTreeiENSGT00390000007840.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiO55004.
KOiK16632.
OMAiSSRAPNC.
OrthoDBiEOG7J1826.
PhylomeDBiO55004.
TreeFamiTF333393.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55004-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIQRTQSLL LLLLLTLLGL GLVQPSYGQD RMYQRFLRQH VDPEGTGGSD
60 70 80 90 100
NYCNVMMQRR RMTSTQCKRF NTFIHEDIWN IRSICDTANI PCKNGNMNCH
110 120 130 140
EGIVRVTDCR ETGSSVPHNC RYRARASTRR VVIACEGTPE VPVHFDR
Length:147
Mass (Da):16,903
Last modified:June 1, 1998 - v1
Checksum:i110D54CF7C691BB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041066 mRNA. Translation: AAC23487.1.
BC070953 mRNA. Translation: AAH70953.1.
RefSeqiNP_064467.1. NM_020082.2.
XP_006251968.1. XM_006251906.2.
XP_006251969.1. XM_006251907.2.
UniGeneiRn.1742.

Genome annotation databases

EnsembliENSRNOT00000041495; ENSRNOP00000043003; ENSRNOG00000025625.
ENSRNOT00000090272; ENSRNOP00000069341; ENSRNOG00000025625.
GeneIDi56759.
KEGGirno:56759.
UCSCiRGD:61823. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041066 mRNA. Translation: AAC23487.1.
BC070953 mRNA. Translation: AAH70953.1.
RefSeqiNP_064467.1. NM_020082.2.
XP_006251968.1. XM_006251906.2.
XP_006251969.1. XM_006251907.2.
UniGeneiRn.1742.

3D structure databases

ProteinModelPortaliO55004.
SMRiO55004. Positions 29-146.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043003.

Proteomic databases

PaxDbiO55004.
PRIDEiO55004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000041495; ENSRNOP00000043003; ENSRNOG00000025625.
ENSRNOT00000090272; ENSRNOP00000069341; ENSRNOG00000025625.
GeneIDi56759.
KEGGirno:56759.
UCSCiRGD:61823. rat.

Organism-specific databases

CTDi6038.
RGDi61823. Rnase4.

Phylogenomic databases

eggNOGiNOG42321.
GeneTreeiENSGT00390000007840.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiO55004.
KOiK16632.
OMAiSSRAPNC.
OrthoDBiEOG7J1826.
PhylomeDBiO55004.
TreeFamiTF333393.

Miscellaneous databases

NextBioi611138.
PROiO55004.

Gene expression databases

ExpressionAtlasiO55004. baseline and differential.
GenevisibleiO55004. RN.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Ribonucleases from rat and bovine liver: purification, specificity and structural characterization."
    Zhao W., Kote-Jarai Z., van Santen Y., Hofsteenge J., Beintema J.J.
    Biochim. Biophys. Acta 1384:55-65(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiRNAS4_RAT
AccessioniPrimary (citable) accession number: O55004
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: July 22, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.