O55004 (RNAS4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 4
Short name=RNase 4
EC=3.1.27.-

Alternative name(s):
RL3

Gene names

Name:

Rnase4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This RNase has marked specificity towards the 3' side of uridine nucleotides.
Subcellular location	Secreted.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	By similarity
	Chain	29 – 147	119	Ribonuclease 4		PRO_0000030887
Regions
	Region	68 – 72	5	Substrate binding By similarity
Sites
	Active site	40	1	Proton acceptor By similarity
	Active site	144	1	Proton donor By similarity
	Binding site	38	1	Substrate By similarity
	Binding site	93	1	Substrate By similarity
	Binding site	110	1	Substrate By similarity
Amino acid modifications
	Modified residue	29	1	Pyrrolidone carboxylic acid By similarity
	Disulfide bond	53 ↔ 109		By similarity
	Disulfide bond	67 ↔ 120		By similarity
	Disulfide bond	85 ↔ 135		By similarity
	Disulfide bond	92 ↔ 99		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O55004 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 110D54CF7C691BB3	FASTA	147	16,903
	10 20 30 40 50 60 MDIQRTQSLL LLLLLTLLGL GLVQPSYGQD RMYQRFLRQH VDPEGTGGSD NYCNVMMQRR 70 80 90 100 110 120 RMTSTQCKRF NTFIHEDIWN IRSICDTANI PCKNGNMNCH EGIVRVTDCR ETGSSVPHNC 130 140 RYRARASTRR VVIACEGTPE VPVHFDR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ribonucleases from rat and bovine liver: purification, specificity and structural characterization." Zhao W., Kote-Jarai Z., van Santen Y., Hofsteenge J., Beintema J.J. Biochim. Biophys. Acta 1384:55-65(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF041066 mRNA. Translation: AAC23487.1. BC070953 mRNA. Translation: AAH70953.1.
IPI	IPI00194925.
RefSeq	NP_064467.1. NM_020082.2.
UniGene	Rn.1742.
3D structure databases
ProteinModelPortal	O55004.
SMR	O55004. Positions 29-146.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000043003.
Proteomic databases
PaxDb	O55004.
PRIDE	O55004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000041495; ENSRNOP00000043003; ENSRNOG00000025625.
GeneID	56759.
KEGG	rno:56759.
UCSC	RGD:61823. rat.
Organism-specific databases
CTD	6038.
RGD	61823. Rnase4.
Phylogenomic databases
eggNOG	NOG42321.
GeneTree	ENSGT00390000007840.
HOGENOM	HOG000276883.
HOVERGEN	HBG008396.
InParanoid	O55004.
KO	K16632.
OMA	SSRAPNC.
OrthoDB	EOG4TMR3F.
Gene expression databases
Genevestigator	O55004.
GermOnline	ENSRNOG00000025625. Rattus norvegicus.
Family and domain databases
Gene3D	3.10.130.10. 1 hit.
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
PANTHER	PTHR11437. PTHR11437. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	611138.

Entry information

Entry name

RNAS4_RAT

Accession

Primary (citable) accession number: O55004

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program