Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

Gene

Bnip3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2 (By similarity). Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane may play a critical role in the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix (By similarity). The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix (By similarity). Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Gene namesi
Name:Bnip3
Synonyms:Nip3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:109326. Bnip3.

Subcellular locationi

  • Mitochondrion
  • Mitochondrion outer membrane By similarity; Single-pass membrane protein By similarity

  • Note: Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei157 – 177HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000649651 – 187BCL2/adenovirus E1B 19 kDa protein-interacting protein 3Add BLAST187

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48PhosphoserineBy similarity1
Modified residuei60PhosphoserineBy similarity1
Modified residuei77PhosphoserineCombined sources1
Modified residuei79PhosphoserineCombined sources1
Modified residuei85PhosphoserineCombined sources1
Modified residuei88PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO55003.
PaxDbiO55003.
PeptideAtlasiO55003.
PRIDEiO55003.

PTM databases

iPTMnetiO55003.
PhosphoSitePlusiO55003.

Expressioni

Gene expression databases

BgeeiENSMUSG00000078566.
CleanExiMM_BNIP3.
GenevisibleiO55003. MM.

Interactioni

Subunit structurei

Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Interacts (via BH3 domain) with SPATA18 (via coiled-coil domains). Interacts with BOK; promotes BOK oligomerization.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198377. 2 interactors.
STRINGi10090.ENSMUSP00000101718.

Structurei

3D structure databases

ProteinModelPortaliO55003.
SMRiO55003.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi93 – 118BH3Add BLAST26

Sequence similaritiesi

Belongs to the NIP3 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHYW. Eukaryota.
ENOG4111KXP. LUCA.
GeneTreeiENSGT00390000013415.
HOGENOMiHOG000232139.
HOVERGENiHBG050707.
InParanoidiO55003.
KOiK15464.
OMAiHPKRSAT.
OrthoDBiEOG091G0REJ.
PhylomeDBiO55003.
TreeFamiTF315424.

Family and domain databases

InterProiIPR010548. BNIP3.
[Graphical view]
PfamiPF06553. BNIP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O55003-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSGEENLQ GSWVELHFSN GNGSSVPASV SIYNGDMEKI LLDAQHESGR
60 70 80 90 100
SSSKSSHCDS PPRSQTPQDT NRAEIDSHSF GEKNSTLSEE DYIERRREVE
110 120 130 140 150
SILKKNSDWI WDWSSRPENI PPKEFLFKHP KRTATLSMRN TSVMKKGGIF
160 170 180
SADFLKVFLP SLLLSHLLAI GLGIYIGRRL TTSTSTF
Length:187
Mass (Da):20,978
Last modified:June 1, 1998 - v1
Checksum:i901BCFACF43EE989
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041054 mRNA. Translation: AAD02922.1.
AK014223 mRNA. Translation: BAB29214.1.
AK075943 mRNA. Translation: BAC36072.1.
AK152610 mRNA. Translation: BAE31356.1.
BC046603 mRNA. Translation: AAH46603.1.
CCDSiCCDS40168.1.
RefSeqiNP_033890.1. NM_009760.4.
UniGeneiMm.378890.

Genome annotation databases

EnsembliENSMUST00000106112; ENSMUSP00000101718; ENSMUSG00000078566.
GeneIDi12176.
KEGGimmu:12176.
UCSCiuc009kfg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041054 mRNA. Translation: AAD02922.1.
AK014223 mRNA. Translation: BAB29214.1.
AK075943 mRNA. Translation: BAC36072.1.
AK152610 mRNA. Translation: BAE31356.1.
BC046603 mRNA. Translation: AAH46603.1.
CCDSiCCDS40168.1.
RefSeqiNP_033890.1. NM_009760.4.
UniGeneiMm.378890.

3D structure databases

ProteinModelPortaliO55003.
SMRiO55003.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198377. 2 interactors.
STRINGi10090.ENSMUSP00000101718.

PTM databases

iPTMnetiO55003.
PhosphoSitePlusiO55003.

Proteomic databases

MaxQBiO55003.
PaxDbiO55003.
PeptideAtlasiO55003.
PRIDEiO55003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000106112; ENSMUSP00000101718; ENSMUSG00000078566.
GeneIDi12176.
KEGGimmu:12176.
UCSCiuc009kfg.1. mouse.

Organism-specific databases

CTDi664.
MGIiMGI:109326. Bnip3.

Phylogenomic databases

eggNOGiENOG410IHYW. Eukaryota.
ENOG4111KXP. LUCA.
GeneTreeiENSGT00390000013415.
HOGENOMiHOG000232139.
HOVERGENiHBG050707.
InParanoidiO55003.
KOiK15464.
OMAiHPKRSAT.
OrthoDBiEOG091G0REJ.
PhylomeDBiO55003.
TreeFamiTF315424.

Miscellaneous databases

PROiO55003.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000078566.
CleanExiMM_BNIP3.
GenevisibleiO55003. MM.

Family and domain databases

InterProiIPR010548. BNIP3.
[Graphical view]
PfamiPF06553. BNIP3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBNIP3_MOUSE
AccessioniPrimary (citable) accession number: O55003
Secondary accession number(s): Q544Y4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.