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Reviewed, UniProtKB/Swiss-Prot O54992 (MAPK5_MOUSE)

Last modified February 9, 2010. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    MAP kinase-activated protein kinase 5
      Short name=MAPK-activated protein kinase 5
      Short name=MAPKAP kinase 5
      Short name=MAPKAPK-5
    EC=2.7.11.1
Gene names
Name: Mapkapk5
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length473 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Mediates stress-induced small heat shock protein 27 phosphorylation By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

In vivo, p38 alpha and beta-dependent phosphorylation increases PRAK activity By similarity. In vitro, ERK also activates MAPKAPK5. Activated by stress-related extracellular stimuli; such as H2O2, arsenite, anisomycin TNF alpha and also PMA and the calcium ionophore A23187; but to a lesser extent. In vitro, activated by SQSTM1 By similarity. Ref.1

Subunit structure

Interacts with SQSTM1 By similarity.

Subcellular location

Cytoplasm Probable. Nucleus By similarity. Note: Also observed in the nucleus By similarity.

Tissue specificity

Expressed ubiquitously. Ref.1

Post-translational modification

Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12 By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological processprotein amino acid phosphorylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from direct assay. Source: MGI

nucleus

Inferred from direct assay. Source: MGI

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction. Source: IntAct

protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HSPB1P047921EBI-1202132,EBI-352682From a different organism.
Tp53P023401EBI-1202132,EBI-474016

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O54992-1)

Also known as: MK-5 type 1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O54992-2)

Also known as: MK-5 type 3;

The sequence of this isoform differs from the canonical sequence as follows:
     344-408: DLKVSLKPLHSVNNPILRKRKLLGTKPKDGIYIHDHENGTEDSNVALEKLRDVIAQCILPQAGKG → E
Isoform 3 (identifier: O54992-3)

Also known as: MK-5 type 4;

The sequence of this isoform differs from the canonical sequence as follows:
     13-161: Missing.
Isoform 4 (identifier: O54992-4)

Also known as: MK-5 type 5;

The sequence of this isoform differs from the canonical sequence as follows:
     13-161: Missing.
     407-408: Missing.
Isoform 5 (identifier: O54992-5)

Also known as: MK-5 type 2;

The sequence of this isoform differs from the canonical sequence as follows:
     407-408: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 473473MAP kinase-activated protein kinase 5
PRO_0000086297

Regions

Domain22 – 304283Protein kinase
Nucleotide binding28 – 369ATP By similarity
Coiled coil409 – 44032 Potential

Sites

Active site1481Proton acceptor By similarity
Binding site511ATP By similarity

Amino acid modifications

Modified residue1821Phosphothreonine By similarity
Modified residue2141Phosphothreonine By similarity

Natural variations

Alternative sequence13 – 161149Missing in isoform 3 and isoform 4.
VSP_011599
Alternative sequence344 – 40865DLKVS…QAGKG → E in isoform 2.
VSP_011600
Alternative sequence407 – 4082Missing in isoform 4 and isoform 5.
VSP_011598

Experimental info

Mutagenesis511K → E: No p38-/ERK-induced activation. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (MK-5 type 1) [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 45441A735075B247

FASTA47354,152
        10         20         30         40         50         60 
MSEDSDMEKA IKETSILEEY SINWTQKLGA GISGPVRVCV KKSTQERFAL KILLDRPKAR 

        70         80         90        100        110        120 
NEVRLHMMCA THPNIVQIIE VFANSVQFPH ESSPRARLLI VMEMMEGGEL FHRISQHRHF 

       130        140        150        160        170        180 
TEKQASQVTK QIALALQHCH LLNIAHRDLK PENLLFKDNS LDAPVKLCDF GFAKVDQGDL 

       190        200        210        220        230        240 
MTPQFTPYYV APQVLEAQRR HQKEKSGIIP TSPTPYTYNK SCDLWSLGVI IYVMLCGYPP 

       250        260        270        280        290        300 
FYSKHHSRTI PKDMRKKIMT GSFEFPEEEW SQISEMAKDV VRKLLKVKPE ERLTIEGVLD 

       310        320        330        340        350        360 
HPWLNSTEAL DNVLPSAQLM MDKAVVAGIQ QAHAEQLANM RIQDLKVSLK PLHSVNNPIL 

       370        380        390        400        410        420 
RKRKLLGTKP KDGIYIHDHE NGTEDSNVAL EKLRDVIAQC ILPQAGKGEN EDEKLNEVMQ 

       430        440        450        460        470 
EAWKYNRECK LLRDALQSFS WNGRGFTDKV DRLKLAEVVK QVIEEQTLPH EPQ 

« Hide

Isoform 2 (MK-5 type 3).

Checksum: 7EEAF061669A3F61
Show »

FASTA40947,103
Isoform 3 (MK-5 type 4).

Checksum: 25BF2CB86528174D
Show »

FASTA32437,042
Isoform 4 (MK-5 type 5).

Checksum: BADC2B6FEB4AEA4A
Show »

FASTA32236,856
Isoform 5 (MK-5 type 2).

Checksum: AC171E324E40A3D4
Show »

FASTA47153,967

References

« Hide 'large scale' references
[1]"MAPKAPK5, a novel mitogen-activated protein kinase (MAPK)-activated protein kinase, is a substrate of the extracellular-regulated kinase (ERK) and p38 kinase."
Ni H., Wang X.S., Diener K., Yao Z.
Biochem. Biophys. Res. Commun. 243:492-496(1998) [PubMed: 9480836] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, MUTAGENESIS OF LYS-51, ENZYME REGULATION.
Tissue: Spleen.
[2]"Novel splice variants of MK5 from mouse heart."
Benoit M.-J., Moise N., Mamarbachi A.M., Allen B.G.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3; 4 AND 5).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N-3.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF039840 mRNA. Translation: AAC40047.1.
AY533679 mRNA. Translation: AAS22330.1.
AY533680 mRNA. Translation: AAS22331.2.
AY533681 mRNA. Translation: AAS22332.1.
AY533682 mRNA. Translation: AAS22333.1.
BC019184 mRNA. Translation: AAH19184.1.
IPIIPI00120068.
IPI00462486.
IPI00462487.
IPI00462488.
IPI00850634.
PIRJC5952.
RefSeqNP_034895.1.
XP_001479292.1.
UniGeneMm.272206

3D structure databases

SMRO54992. Positions 11-362.
ModBaseSearch...

Protein-protein interaction databases

IntActO54992. 2 interactions.
STRINGO54992.

PTM databases

PhosphoSiteO54992.

Proteomic databases

PRIDEO54992.

Genome annotation databases

EnsemblENSMUST00000031410; ENSMUSP00000031410; ENSMUSG00000029454; Mus musculus. [Genome view]
ENSMUST00000111786; ENSMUSP00000107416; ENSMUSG00000029454; Mus musculus. [Genome view]
ENSMUST00000111787; ENSMUSP00000107417; ENSMUSG00000029454; Mus musculus. [Genome view]
GeneID17165.
KEGGmmu:100047833.
mmu:17165.
UCSCuc008zjq.1. mouse.
uc008zjr.1. mouse.
uc008zjs.1. mouse.

Organism-specific databases

CTD17165.
MGIMGI:1333110. Mapkapk5.

Phylogenomic databases

eggNOGroNOG12773.
HOGENOMHBG755340.
HOVERGENO54992.
InParanoidO54992.
OMAEYSINWT.

Enzyme and pathway databases

BRENDA2.7.11.1. 244.

Gene expression databases

ArrayExpressO54992.
BgeeO54992.
CleanExMM_MAPKAPK5.
GenevestigatorO54992.
GermOnlineENSMUSG00000029454. Mus musculus.

Family and domain databases

InterProIPR020636. Ca/CaM-dep_prot_kinase-like.
IPR011009. Kinase-like_dom.
IPR020646. MAP_kin-act_prot_kinase.
IPR000719. Prot_kinase_cat_dom.
IPR017442. Se/Thr_prot_kinase-like_dom.
IPR008271. Ser/Thr_prot_kinase_AS.
IPR002290. Ser/Thr_prot_kinase_dom.
[Graphical view]
PANTHERPTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit.
PTHR22982:SF22. MAP_kin-act_prot_kinase. 1 hit.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio291446.
SOURCESearch...

Entry information

Entry nameMAPK5_MOUSE
AccessionPrimary (citable) accession number: O54992
Secondary accession number(s): Q6QME4 expand/collapse secondary AC list , Q6QME5, Q6QME6, Q6QME7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: June 1, 1998
Last modified: February 9, 2010
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents